NafoA.01534.b

adp-ribosylation factor-like protein 1-like

CENTER ID:	NafoA.01534.b
ORGANISM:	Naegleria fowleri ATCC 30863
ASSOCIATED DISEASE:
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
NafoA.01534.b.B1.GE40540	Full length(NafoA.01534.b)		1	182

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	AmoebaDB:NF0071690

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MGNLIASIFG GLFNMEQEVR ILILGLDNAG KTTILYRLQV GEAVTTIPTI GFNVETITYK NIKFQVWDLG GQTSIRPYWR CYYANTDAII YVVDSSDRDR MGIAKEELFA MLDEKELKDA ILLIMANKQD AKNAMTVEQV SEQLNLSKIK NRTWTIFKTS ALNGIGINEA LEWLSENIKN RK

NT Sequence

ATGGGCAATT TAATAGCAAG TATCTTTGGT GGCTTATTTA ATATGGAACA AGAAGTCCGT ATTTTGATTT TAGGACTTGA TAACGCCGGA AAAACCACAA TTTTATATCG GTTACAAGTC GGAGAGGCAG TAACAACAAT ACCTACGATC GGATTTAACG TAGAAACCAT TACATATAAA AATATTAAAT TTCAGGTGTG GGATTTAGGA GGTCAAACAT CCATTAGACC CTATTGGCGA TGCTATTATG CAAATACGGA TGCTATAATT TATGTGGTAG ATTCAAGTGA TAGAGATAGA ATGGGTATTG CAAAAGAAGA GCTATTTGCA ATGCTGGATG AAAAGGAGCT CAAAGACGCT ATTCTTTTAA TTATGGCAAA CAAACAGGAT GCTAAAAACG CAATGACTGT GGAACAAGTT TCCGAACAAC TCAATTTAAG CAAGATAAAA AACAGAACAT GGACAATATT TAAAACATCA GCACTGAATG GCATTGGTAT AAACGAAGCT TTAGAGTGGC TCAGTGAAAA TATAAAGAAT AGAAAA

Details for NafoA.01534.b.B1.GE40540

HARVESTED ON:	9/1/2016
SEQUENCED ON:	9/8/2016
EXPECTED MW:	22kDa
OBSERVED MW:	22kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	100

Validated AA Sequence

MAHHHHHHMG NLIASIFGGL FNMEQEVRIL ILGLDNAGKT TILYRLQVGE AVTTIPTIGF NVETITYKNI KFQVWDLGGQ TSIRPYWRCY YANTDAIIYV VDSSDRDRMG IAKEELFAML DEKELKDAIL LIMANKQDAK NAMTVEQVSE QLNLSKIKNR TWTIFKTSAL NGIGINEALE WLSENIKNRK

Validated NT Sequence

atggctcacc accaccacca ccatatgggc aatttaatag caagtatctt tggtggctta tttaatatgg aacaagaagt ccgtattttg attttaggac ttgataacgc cggaaaaacc acaattttat atcggttaca agtcggagag gcagtaacaa caatacctac gatcggattt aacgtagaaa ccattacata taaaaatatt aaatttcagg tgtgggattt aggaggtcaa acatccatta gaccctattg gcgatgctat tatgcaaata cggatgctat aatttatgtg gtagattcaa gtgatagaga tagaatgggt attgcaaaag aagagctatt tgcaatgctg gatgaaaagg agctcaaaga cgctattctt ttaattatgg caaacaaaca ggatgctaaa aacgcaatga ctgtggaaca agtttccgaa caactcaatt taagcaagat aaaaaacaga acatggacaa tatttaaaac atcagcactg aatggcattg gtataaacga agctttagag tggctcagtg aaaatataaa gaatagaaaa taagtgagta agat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgggca atttaatagc aagtatcttt ggtggcttat ttaatatgga acaagaagtc cgtattttga ttttaggact tgataacgcc ggaaaaacca caattttata tcggttacaa gtcggagagg cagtaacaac aatacctacg atcggattta acgtagaaac cattacatat aaaaatatta aatttcaggt gtgggattta ggaggtcaaa catccattag accctattgg cgatgctatt atgcaaatac ggatgctata atttatgtgg tagattcaag tgatagagat agaatgggta ttgcaaaaga agagctattt gcaatgctgg atgaaaagga gctcaaagac gctattcttt taattatggc aaacaaacag gatgctaaaa acgcaatgac tgtggaacaa gtttccgaac aactcaattt aagcaagata aaaaacagaa catggacaat atttaaaaca tcagcactga atggcattgg tataaacgaa gctttagagt ggctcagtga aaatataaag aatagaaaat gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat