NafoA.01523.a
protein arginine n-methyltransferase
| CENTER ID: | NafoA.01523.a |
| ORGANISM: | Naegleria fowleri ATCC 30863 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| NafoA.01523.a.B1.GE40538 | Full length(NafoA.01523.a) | 1 | 328 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| NafoA.01523.a.B1.PW37976 | Full length(NafoA.01523.a) | 1 | 328 |
Structures
6CU3
DEPOSITED: 3/23/2018
DETERMINATION: XRay
CLONE: NafoA.01523.a.B1.GE40538
PROTEIN: NafoA.01523.a.B1.PW37976
6CU5
DEPOSITED: 3/23/2018
DETERMINATION: XRay
CLONE: NafoA.01523.a.B1.GE40538
PROTEIN: NafoA.01523.a.B1.PW37976
Publications by SSGCID
Naegleria fowleri: Protein structures to facilitate drug discovery for the deadly, pathogenic free-living amoeba
Abendroth J, Barrett LK, Dranow DM, Edwards TE, Myler PJ, Phan IQ, Staker B, Subramanian S, Tran N, Van Voorhis WC, Craig JC, Smith C, Chun IKH, Kyle D, Barrett KF, Morris JC, Tillery L, Rice CA, Goldstein J, Lassner JW, Xu L, Young RM, Osterhout B, Leahy JW, Manetsch R
PLoS ONE - 2021
volume 16, issue 3, pages e0241738
PMID: 33760815; PMCID: PMC7990177
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| EuPathDB: | AmoebaDB:NF0022020 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNDFHHDFQQ QQPTTGIHEE MLKDGIRTNA YKNAILQNKH LFKDKVVLDI GCGTGILCLF AAKAGAKRVI GIDMSDIIDK ARQIVSDNGY SHVIELIKGK VEDIAQLPFG IEKVDIIISE WMGYFLLYES MLQTVLSARD RWLRPGGYLF PDKCTMYICG IEDSEYKRDK IDFWDNVYGF NFSAIKADAL REPLVDFVES QQIITTQSKF LEIDLNTIQP EDLKQITTSF EFTSQYQEYC QAFVAWFDCV FSRGPHKPVE FSTGPFTEGT HWKQTVFYLE NDLPLKPNDV IKGTITISQN KSNHRDLDIS MKYTVNGGAV ISQDYIMR
NT Sequence
ATGAACGATT TTCATCATGA TTTTCAACAA CAACAACCAA CAACAGGAAT TCATGAGGAG ATGCTCAAGG ATGGTATAAG AACCAATGCC TACAAGAATG CAATTCTCCA AAACAAACAT CTTTTCAAAG ATAAGGTTGT TCTTGACATT GGATGCGGAA CAGGTATTTT GTGCCTCTTT GCAGCAAAGG CTGGTGCCAA ACGAGTGATT GGTATTGATA TGAGTGATAT CATTGATAAA GCAAGACAAA TTGTTTCCGA TAATGGTTAT TCTCATGTGA TTGAGTTAAT CAAGGGTAAA GTTGAGGACA TTGCTCAATT ACCTTTCGGA ATTGAGAAAG TAGATATCAT CATCAGTGAA TGGATGGGAT ACTTTTTATT GTACGAGAGT ATGTTACAGA CTGTTCTCAG TGCTCGTGAT AGATGGTTGA GACCAGGTGG CTATCTTTTC CCTGACAAAT GCACCATGTA CATTTGTGGT ATTGAAGATT CAGAATATAA GAGGGATAAG ATTGATTTCT GGGACAATGT CTATGGATTT AATTTCAGTG CTATTAAAGC AGATGCATTG AGAGAACCTT TGGTTGACTT TGTTGAGTCA CAACAAATCA TTACTACTCA ATCCAAGTTT TTAGAGATTG ATTTGAACAC TATTCAACCT GAAGATTTGA AGCAAATTAC TACCAGCTTT GAATTTACAT CACAATATCA AGAATATTGC CAAGCATTTG TTGCTTGGTT TGATTGTGTC TTTTCTCGAG GTCCTCACAA ACCTGTTGAA TTCTCCACTG GACCATTTAC TGAAGGCACT CACTGGAAAC AAACAGTTTT CTATTTAGAA AATGATTTAC CATTGAAACC TAACGATGTG ATTAAGGGTA CCATCACCAT TTCTCAGAAC AAGAGCAATC ATCGTGACTT GGATATTTCC ATGAAGTATA CAGTGAATGG TGGAGCCGTA ATCTCTCAAG ACTATATTAT GAGA
Details for NafoA.01523.a.B1.GE40538
| HARVESTED ON: | 9/1/2016 |
| SEQUENCED ON: | 9/8/2016 |
| EXPECTED MW: | 39kDa |
| OBSERVED MW: | 39kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 97 |
Validated AA Sequence
MAHHHHHMND FHHDFQQQQP TTGIHEEMLK DGIRTNAYKN AILQNKHLFK DKVVLDIGCG TGILCLFAAK AGAKRVIGID MSDIIDKARQ IVSDNGYSHV IELIKGKVED IAQLPFGIEK VDIIISEWMG YFLLYESMLQ TVLSARDRWL RPGGYLFPDK CTMYICGIED SEYKRDKIDF WDNVYGFNFS AIKADALREP LVDFVESQQI ITTQSKFLEI DLNTIQPEDL KQITTSFEFT SQYQEYCQAF VAWFDCVFSR GPHKPVEFST GPFTEGTHWK QTVFYLENDL PLKPNDVIKG TITISQNKSN HRDLDISMKY TVNGGA
Validated NT Sequence
atggctcacc accaccacca tatgaacgat tttcatcatg attttcaaca acaacaacca acaacaggaa ttcatgagga gatgctcaag gatggtataa gaaccaatgc ctacaagaat gcaattctcc aaaacaaaca tcttttcaaa gataaggttg ttcttgacat tggatgcgga acaggtattt tgtgcctctt tgcagcaaag gctggtgcca aacgagtgat tggtattgat atgagtgata tcattgataa agcaagacaa attgtttccg ataatggtta ttctcatgtg attgagttaa tcaagggtaa agttgaggac attgctcaat tacctttcgg aattgagaaa gtagatatca tcatcagtga atggatggga tactttttat tgtacgagag tatgttacag actgttctca gtgctcgtga tagatggttg agaccaggtg gctatctttt ccctgacaaa tgcaccatgt acatttgtgg tattgaagat tcagaatata agagggataa gattgatttc tgggacaatg tctatggatt taatttcagt gctattaaag cagatgcatt gagagaacct ttggttgact ttgttgagtc acaacaaatc attactactc aatccaagtt tttagagatt gatttgaaca ctattcaacc tgaagatttg aagcaaatta ctaccagctt tgaatttaca tcacaatatc aagaatattg ccaagcattt gttgcttggt ttgattgtgt cttttctcga ggtcctcaca aacctgttga attctccact ggaccattta ctgaaggcac tcactggaaa caaacagttt tctatttaga aaatgattta ccattgaaac ctaacgatgt gattaagggt accatcacca tttctcagaa caagagcaat catcgtgact tggatatttc catgaagtat acagtgaatg gtggagccgt a
Expected Protein Sequence
MAHHHHHHMN DFHHDFQQQQ PTTGIHEEML KDGIRTNAYK NAILQNKHLF KDKVVLDIGC GTGILCLFAA KAGAKRVIGI DMSDIIDKAR QIVSDNGYSH VIELIKGKVE DIAQLPFGIE KVDIIISEWM GYFLLYESML QTVLSARDRW LRPGGYLFPD KCTMYICGIE DSEYKRDKID FWDNVYGFNF SAIKADALRE PLVDFVESQQ IITTQSKFLE IDLNTIQPED LKQITTSFEF TSQYQEYCQA FVAWFDCVFS RGPHKPVEFS TGPFTEGTHW KQTVFYLEND LPLKPNDVIK GTITISQNKS NHRDLDISMK YTVNGGAVIS QDYIMR
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaacg attttcatca tgattttcaa caacaacaac caacaacagg aattcatgag gagatgctca aggatggtat aagaaccaat gcctacaaga atgcaattct ccaaaacaaa catcttttca aagataaggt tgttcttgac attggatgcg gaacaggtat tttgtgcctc tttgcagcaa aggctggtgc caaacgagtg attggtattg atatgagtga tatcattgat aaagcaagac aaattgtttc cgataatggt tattctcatg tgattgagtt aatcaagggt aaagttgagg acattgctca attacctttc ggaattgaga aagtagatat catcatcagt gaatggatgg gatacttttt attgtacgag agtatgttac agactgttct cagtgctcgt gatagatggt tgagaccagg tggctatctt ttccctgaca aatgcaccat gtacatttgt ggtattgaag attcagaata taagagggat aagattgatt tctgggacaa tgtctatgga tttaatttca gtgctattaa agcagatgca ttgagagaac ctttggttga ctttgttgag tcacaacaaa tcattactac tcaatccaag tttttagaga ttgatttgaa cactattcaa cctgaagatt tgaagcaaat tactaccagc tttgaattta catcacaata tcaagaatat tgccaagcat ttgttgcttg gtttgattgt gtcttttctc gaggtcctca caaacctgtt gaattctcca ctggaccatt tactgaaggc actcactgga aacaaacagt tttctattta gaaaatgatt taccattgaa acctaacgat gtgattaagg gtaccatcac catttctcag aacaagagca atcatcgtga cttggatatt tccatgaagt atacagtgaa tggtggagcc gtaatctctc aagactatat tatgagatga gtaagatagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaattcttga agacgaaagg gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg atcagcggtc cagtgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatctcg atcccgcgaa at
Details for NafoA.01523.a.B1.PW37976
| PURIFICATION DATe: | 11/15/2016 |
| CONCENTRATION: | 5.5mg/ml |
| OBSERVED MW: | 36kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| VIAL COUNT (approx.): | 18 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 97 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHMND FHHDFQQQQP TTGIHEEMLK DGIRTNAYKN AILQNKHLFK DKVVLDIGCG TGILCLFAAK AGAKRVIGID MSDIIDKARQ IVSDNGYSHV IELIKGKVED IAQLPFGIEK VDIIISEWMG YFLLYESMLQ TVLSARDRWL RPGGYLFPDK CTMYICGIED SEYKRDKIDF WDNVYGFNFS AIKADALREP LVDFVESQQI ITTQSKFLEI DLNTIQPEDL KQITTSFEFT SQYQEYCQAF VAWFDCVFSR GPHKPVEFST GPFTEGTHWK QTVFYLENDL PLKPNDVIKG TITISQNKSN HRDLDISMKY TVNGGA
Validated NT Sequence
atggctcacc accaccacca tatgaacgat tttcatcatg attttcaaca acaacaacca acaacaggaa ttcatgagga gatgctcaag gatggtataa gaaccaatgc ctacaagaat gcaattctcc aaaacaaaca tcttttcaaa gataaggttg ttcttgacat tggatgcgga acaggtattt tgtgcctctt tgcagcaaag gctggtgcca aacgagtgat tggtattgat atgagtgata tcattgataa agcaagacaa attgtttccg ataatggtta ttctcatgtg attgagttaa tcaagggtaa agttgaggac attgctcaat tacctttcgg aattgagaaa gtagatatca tcatcagtga atggatggga tactttttat tgtacgagag tatgttacag actgttctca gtgctcgtga tagatggttg agaccaggtg gctatctttt ccctgacaaa tgcaccatgt acatttgtgg tattgaagat tcagaatata agagggataa gattgatttc tgggacaatg tctatggatt taatttcagt gctattaaag cagatgcatt gagagaacct ttggttgact ttgttgagtc acaacaaatc attactactc aatccaagtt tttagagatt gatttgaaca ctattcaacc tgaagatttg aagcaaatta ctaccagctt tgaatttaca tcacaatatc aagaatattg ccaagcattt gttgcttggt ttgattgtgt cttttctcga ggtcctcaca aacctgttga attctccact ggaccattta ctgaaggcac tcactggaaa caaacagttt tctatttaga aaatgattta ccattgaaac ctaacgatgt gattaagggt accatcacca tttctcagaa caagagcaat catcgtgact tggatatttc catgaagtat acagtgaatg gtggagccgt a
Expressed Protein Sequence
MAHHHHHHMN DFHHDFQQQQ PTTGIHEEML KDGIRTNAYK NAILQNKHLF KDKVVLDIGC GTGILCLFAA KAGAKRVIGI DMSDIIDKAR QIVSDNGYSH VIELIKGKVE DIAQLPFGIE KVDIIISEWM GYFLLYESML QTVLSARDRW LRPGGYLFPD KCTMYICGIE DSEYKRDKID FWDNVYGFNF SAIKADALRE PLVDFVESQQ IITTQSKFLE IDLNTIQPED LKQITTSFEF TSQYQEYCQA FVAWFDCVFS RGPHKPVEFS TGPFTEGTHW KQTVFYLEND LPLKPNDVIK GTITISQNKS NHRDLDISMK YTVNGGAVIS QDYIMR
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAACG ATTTTCATCA TGATTTTCAA CAACAACAAC CAACAACAGG AATTCATGAG GAGATGCTCA AGGATGGTAT AAGAACCAAT GCCTACAAGA ATGCAATTCT CCAAAACAAA CATCTTTTCA AAGATAAGGT TGTTCTTGAC ATTGGATGCG GAACAGGTAT TTTGTGCCTC TTTGCAGCAA AGGCTGGTGC CAAACGAGTG ATTGGTATTG ATATGAGTGA TATCATTGAT AAAGCAAGAC AAATTGTTTC CGATAATGGT TATTCTCATG TGATTGAGTT AATCAAGGGT AAAGTTGAGG ACATTGCTCA ATTACCTTTC GGAATTGAGA AAGTAGATAT CATCATCAGT GAATGGATGG GATACTTTTT ATTGTACGAG AGTATGTTAC AGACTGTTCT CAGTGCTCGT GATAGATGGT TGAGACCAGG TGGCTATCTT TTCCCTGACA AATGCACCAT GTACATTTGT GGTATTGAAG ATTCAGAATA TAAGAGGGAT AAGATTGATT TCTGGGACAA TGTCTATGGA TTTAATTTCA GTGCTATTAA AGCAGATGCA TTGAGAGAAC CTTTGGTTGA CTTTGTTGAG TCACAACAAA TCATTACTAC TCAATCCAAG TTTTTAGAGA TTGATTTGAA CACTATTCAA CCTGAAGATT TGAAGCAAAT TACTACCAGC TTTGAATTTA CATCACAATA TCAAGAATAT TGCCAAGCAT TTGTTGCTTG GTTTGATTGT GTCTTTTCTC GAGGTCCTCA CAAACCTGTT GAATTCTCCA CTGGACCATT TACTGAAGGC ACTCACTGGA AACAAACAGT TTTCTATTTA GAAAATGATT TACCATTGAA ACCTAACGAT GTGATTAAGG GTACCATCAC CATTTCTCAG AACAAGAGCA ATCATCGTGA CTTGGATATT TCCATGAAGT ATACAGTGAA TGGTGGAGCC GTAATCTCTC AAGACTATAT TATGAGATGA GTAAGATAGG ATCCGGCTGC TAACAAAGCC CGAAAGGAAG CTGAGTTGGC TGCTGCCACC GCTGAGCAAT AACTAGCATA ACCCCTTGGG GCCTCTAAAC GGGTCTTGAG GGGTTTTTTG CTGAAAGGAG GAACTATATC CGGATATCCA CAGGACGGGT GTGGTCGCCA TGATCGCGTA GTCGATAGTG GCTCCAAGTA GCGAAGCGAG CAGGACTGGG CGGCGGCCAA AGCGGTCGGA CAGTGCTCCG AGAACGGGTG CGCATAGAAA TTGCATCAAC GCATATAGCG CTAGCAGCAC GCCATAGTGA CTGGCGATGC TGTCGGAATG GACGATATCC CGCAAGAGGC CCGGCAGTAC CGGCATAACC AAGCCTATGC CTACAGCATC CAGGGTGACG GTGCCGAGGA TGACGATGAG CGCATTGTTA GATTTCATAC ACGGTGCCTG ACTGCGTTAG CAATTTAACT GTGATAAACT ACCGCATTAA AGCTTATCGA TGATAAGCTG TCAAACATGA GAATTCTTGA AGACGAAAGG GCCTCGTGAT ACGCCTATTT TTATAGGTTA ATGTCATGAT AATAATGGTT TCTTAGACGT CAGGTGGCAC TTTTCGGGGA AATGTGCGCG GAACCCCTAT TTGTTTATTT TTCTAAATAC ATTCAAATAT GTATCCGCTC ATGAGACAAT AACCCTGATA AATGCTTCAA TAATATTGAA AAAGGAAGAG TATGAGTATT CAACATTTCC GTGTCGCCCT TATTCCCTTT TTTGCGGCAT TTTGCCTTCC TGTTTTTGCT CACCCAGAAA CGCTGGTGAA AGTAAAAGAT GCTGAAGATC AGTTGGGTGC ACGAGTGGGT TACATCGAAC TGGATCTCAA CAGCGGTAAG ATCCTTGAGA GTTTTCGCCC CGAAGAACGT TTTCCAATGA TGAGCACTTT TAAAGTTCTG CTATGTGGCG CGGTATTATC CCGTGTTGAC GCCGGGCAAG AGCAACTCGG TCGCCGCATA CACTATTCTC AGAATGACTT GGTTGAGTAC TCACCAGTCA CAGAAAAGCA TCTTACGGAT GGCATGACAG TAAGAGAATT ATGCAGTGCT GCCATAACCA TGAGTGATAA CACTGCGGCC AACTTACTTC TGACAACGAT CGGAGGACCG AAGGAGCTAA CCGCTTTTTT GCACAACATG GGGGATCATG TAACTCGCCT TGATCGTTGG GAACCGGAGC TGAATGAAGC CATACCAAAC GACGAGCGTG ACACCACGAT GCCTGCAGCA ATGGCAACAA CGTTGCGCAA ACTATTAACT GGCGAACTAC TTACTCTAGC TTCCCGGCAA CAATTAATAG ACTGGATGGA GGCGGATAAA GTTGCAGGAC CACTTCTGCG CTCGGCCCTT CCGGCTGGCT GGTTTATTGC TGATAAATCT GGAGCCGGTG AGCGTGGGTC TCGCGGTATC ATTGCAGCAC TGGGGCCAGA TGGTAAGCCC TCCCGTATCG TAGTTATCTA CACGACGGGG AGTCAGGCAA CTATGGATGA ACGAAATAGA CAGATCGCTG AGATAGGTGC CTCACTGATT AAGCATTGGT AACTGTCAGA CCAAGTTTAC TCATATATAC TTTAGATTGA TTTAAAACTT CATTTTTAAT TTAAAAGGAT CTAGGTGAAG ATCCTTTTTG ATAATCTCAT GACCAAAATC CCTTAACGTG AGTTTTCGTT CCACTGAGCG TCAGACCCCG TAGAAAAGAT CAAAGGATCT TCTTGAGATC CTTTTTTTCT GCGCGTAATC TGCTGCTTGC AAACAAAAAA ACCACCGCTA CCAGCGGTGG TTTGTTTGCC GGATCAAGAG CTACCAACTC TTTTTCCGAA GGTAACTGGC TTCAGCAGAG CGCAGATACC AAATACTGTC CTTCTAGTGT AGCCGTAGTT AGGCCACCAC TTCAAGAACT CTGTAGCACC GCCTACATAC CTCGCTCTGC TAATCCTGTT ACCAGTGGCT GCTGCCAGTG GCGATAAGTC GTGTCTTACC GGGTTGGACT CAAGACGATA GTTACCGGAT AAGGCGCAGC GGTCGGGCTG AACGGGGGGT TCGTGCACAC AGCCCAGCTT GGAGCGAACG ACCTACACCG AACTGAGATA CCTACAGCGT GAGCTATGAG AAAGCGCCAC GCTTCCCGAA GGGAGAAAGG CGGACAGGTA TCCGGTAAGC GGCAGGGTCG GAACAGGAGA GCGCACGAGG GAGCTTCCAG GGGGAAACGC CTGGTATCTT TATAGTCCTG TCGGGTTTCG CCACCTCTGA CTTGAGCGTC GATTTTTGTG ATGCTCGTCA GGGGGGCGGA GCCTATGGAA AAACGCCAGC AACGCGGCCT TTTTACGGTT CCTGGCCTTT TGCTGGCCTT TTGCTCACAT GTTCTTTCCT GCGTTATCCC CTGATTCTGT GGATAACCGT ATTACCGCCT TTGAGTGAGC TGATACCGCT CGCCGCAGCC GAACGACCGA GCGCAGCGAG TCAGTGAGCG AGGAAGCGGA AGAGCGCCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC GGTATTTCAC ACCGCATATA TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT TAAGCCAGTA TACACTCCGC TATCGCTACG TGACTGGGTC ATGGCTGCGC CCCGACACCC GCCAACACCC GCTGACGCGC CCTGACGGGC TTGTCTGCTC CCGGCATCCG CTTACAGACA AGCTGTGACC GTCTCCGGGA GCTGCATGTG TCAGAGGTTT TCACCGTCAT CACCGAAACG CGCGAGGCAG CTGCGGTAAA GCTCATCAGC GTGGTCGTGA AGCGATTCAC AGATGTCTGC CTGTTCATCC GCGTCCAGCT CGTTGAGTTT CTCCAGAAGC GTTAATGTCT GGCTTCTGAT AAAGCGGGCC ATGTTAAGGG CGGTTTTTTC CTGTTTGGTC ACTGATGCCT CCGTGTAAGG GGGATTTCTG TTCATGGGGG TAATGATACC GATGAAACGA GAGAGGATGC TCACGATACG GGTTACTGAT GATGAACATG CCCGGTTACT GGAACGTTGT GAGGGTAAAC AACTGGCGGT ATGGATGCGG CGGGACCAGA GAAAAATCAC TCAGGGTCAA TGCCAGCGCT TCGTTAATAC AGATGTAGGT GTTCCACAGG GTAGCCAGCA GCATCCTGCG ATGCAGATCC GGAACATAAT GGTGCAGGGC GCTGACTTCC GCGTTTCCAG ACTTTACGAA ACACGGAAAC CGAAGACCAT TCATGTTGTT GCTCAGGTCG CAGACGTTTT GCAGCAGCAG TCGCTTCACG TTCGCTCGCG TATCGGTGAT TCATTCTGCT AACCAGTAAG GCAACCCCGC CAGCCTAGCC GGGTCCTCAA CGACAGGAGC ACGATCATGC GCACCCGTGG CCAGGACCCA ACGCTGCCCG AGATGCGCCG CGTGCGGCTG CTGGAGATGG CGGACGCGAT GGATATGTTC TGCCAAGGGT TGGTTTGCGC ATTCACAGTT CTCCGCAAGA ATTGATTGGC TCCAATTCTT GGAGTGGTGA ATCCGTTAGC GAGGTGCCGC CGGCTTCCAT TCAGGTCGAG GTGGCCCGGC TCCATGCACC GCGACGCAAC GCGGGGAGGC AGACAAGGTA TAGGGCGGCG CCTACAATCC ATGCCAACCC GTTCCATGTG CTCGCCGAGG CGGCATAAAT CGCCGTGACG ATCAGCGGTC CAGTGATCGA AGTTAGGCTG GTAAGAGCCG CGAGCGATCC TTGAAGCTGT CCCTGATGGT CGTCATCTAC CTGCCTGGAC AGCATGGCCT GCAACGCGGG CATCCCGATG CCGCCGGAAG CGAGAAGAAT CATAATGGGG AAGGCCATCC AGCCTCGCGT CGCGAACGCC AGCAAGACGT AGCCCAGCGC GTCGGCCGCC ATGCCGGCGA TAATGGCCTG CTTCTCGCCG AAACGTTTGG TGGCGGGACC AGTGACGAAG GCTTGAGCGA GGGCGTGCAA GATTCCGAAT ACCGCAAGCG ACAGGCCGAT CATCGTCGCG CTCCAGCGAA AGCGGTCCTC GCCGAAAATG ACCCAGAGCG CTGCCGGCAC CTGTCCTACG AGTTGCATGA TAAAGAAGAC AGTCATAAGT GCGGCGACGA TAGTCATGCC CCGCGCCCAC CGGAAGGAGC TGACTGGGTT GAAGGCTCTC AAGGGCATCG GTCGACGCTC TCCCTTATGC GACTCCTGCA TTAGGAAGCA GCCCAGTAGT AGGTTGAGGC CGTTGAGCAC CGCCGCCGCA AGGAATGGTG CATGCAAGGA GATGGCGCCC AACAGTCCCC CGGCCACGGG GCCTGCCACC ATACCCACGC CGAAACAAGC GCTCATGAGC CCGAAGTGGC GAGCCCGATC TTCCCCATCG GTGATGTCGG CGATATAGGC GCCAGCAACC GCACCTGTGG CGCCGGTGAT GCCGGCCACG ATGCGTCCGG CGTAGAGGAT CGAGATCTCG ATCCCGCGAA AT