NafoA.01385.a
rab gdp dissociation inhibitor alpha
| CENTER ID: | NafoA.01385.a |
| ORGANISM: | Naegleria fowleri ATCC 30863 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIB |
Ordering Clones & Proteins
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| NafoA.01385.a.B1.GE40623 | Full length(NafoA.01385.a) | 1 | 444 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| NafoA.01385.a.B1.PS38253 | Full length(NafoA.01385.a) | 1 | 444 |
Structures
6C87
DEPOSITED: 1/24/2018
DETERMINATION: XRay
CLONE: NafoA.01385.a.B1.GE40623
PROTEIN: NafoA.01385.a.B1.PS38253
Publications by SSGCID
Naegleria fowleri: Protein structures to facilitate drug discovery for the deadly, pathogenic free-living amoeba
Abendroth J, Barrett LK, Dranow DM, Edwards TE, Myler PJ, Phan IQ, Staker B, Subramanian S, Tran N, Van Voorhis WC, Craig JC, Smith C, Chun IKH, Kyle D, Barrett KF, Morris JC, Tillery L, Rice CA, Goldstein J, Lassner JW, Xu L, Young RM, Osterhout B, Leahy JW, Manetsch R
PLoS ONE - 2021
volume 16, issue 3, pages e0241738
PMID: 33760815; PMCID: PMC7990177
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| EuPathDB: | AmoebaDB:NF0049320 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MNEEYDVVVL GTGLTECVIS GLLSVSGKKV LHMDRNPYYG GESASLNLDQ MFEKFRGQDA KPPASLGRSR DYNIDLIPKF LMANGKLVKI LRMTGVTRYN MEFALVEGSF VYHKGEIHKV PITPTEVAKT PLLGFFEKLK AKKLVSYLYD YDQNNPKTHQ GFDCSKDTMD KIYKYYGVSE DTTDFLGHAV ALYTDDSYMT TVPALEVIER MRLYEDSLNM YGKSPYVYPM YGLGELPQVF ARLCAVYGGT YMLDKKVDRI VYDDNGHVVG VESGGEVAKC KMVVGDPSYF PEKVRKTGKV IRVICILSHP VKSTENAKSS QIIFPQKQTG RKHDIYCCVT SFTHHVAPNG KYIAIVSTTV ESDNPENEVK VVLDLLNPID EKFVYILDTY APLEDGKKDG VFISKSYDAT THFESCAVDI VDIYERITGE KFDWNKKPVE PQEQ
NT Sequence
ATGAATGAAG AATATGATGT GGTCGTTTTA GGTACTGGTT TAACCGAATG TGTAATTTCA GGTCTTCTCT CCGTGAGTGG TAAGAAGGTC CTCCACATGG ATCGTAACCC TTACTATGGA GGTGAATCTG CTTCTCTCAA CCTGGATCAA ATGTTTGAAA AGTTTAGAGG ACAAGATGCC AAGCCTCCTG CCTCTCTCGG TCGTAGCAGA GATTATAATA TTGATTTAAT TCCAAAATTC TTGATGGCCA ATGGTAAACT CGTAAAGATC TTGAGAATGA CTGGTGTTAC TCGTTATAAT ATGGAATTTG CTTTGGTGGA AGGAAGCTTT GTCTATCACA AGGGAGAAAT CCACAAGGTG CCAATTACTC CAACCGAAGT TGCTAAAACA CCCTTGTTGG GCTTCTTTGA GAAATTGAAG GCTAAAAAGC TTGTTTCCTA TTTGTACGAT TATGATCAAA ACAATCCAAA GACTCATCAA GGGTTTGATT GTTCCAAAGA CACGATGGAT AAGATCTACA AGTATTATGG TGTGAGTGAG GACACTACTG ACTTTTTGGG ACATGCTGTT GCCTTGTACA CAGATGATTC CTACATGACT ACTGTTCCTG CTCTTGAAGT TATTGAGAGA ATGAGATTGT ACGAAGATTC ACTCAATATG TACGGAAAGT CTCCATACGT TTATCCAATG TATGGTTTGG GTGAATTGCC ACAAGTTTTT GCTCGTTTGT GTGCTGTCTA TGGAGGTACA TACATGTTGG ATAAGAAGGT TGATCGAATT GTCTACGATG ACAATGGTCA TGTTGTTGGT GTTGAATCGG GAGGTGAAGT TGCTAAATGT AAGATGGTTG TCGGAGATCC AAGCTACTTC CCTGAGAAGG TTCGCAAGAC TGGAAAGGTT ATTCGTGTCA TTTGCATTCT TTCTCATCCT GTGAAGAGCA CTGAGAATGC CAAGTCTTCA CAAATTATTT TCCCACAAAA GCAAACTGGA AGAAAGCATG ATATTTATTG TTGTGTGACA TCATTCACTC ACCACGTTGC TCCAAATGGC AAATATATTG CCATTGTTTC TACAACAGTT GAGTCAGACA ATCCAGAGAA CGAAGTTAAA GTTGTTCTCG ATCTTTTGAA CCCAATTGAT GAGAAGTTTG TTTACATTCT CGACACTTAT GCTCCATTAG AGGACGGAAA GAAGGATGGT GTTTTCATTT CTAAATCCTA TGATGCCACT ACTCACTTTG AATCATGTGC TGTTGACATT GTTGATATCT ATGAGAGAAT TACTGGTGAG AAGTTTGATT GGAATAAGAA GCCTGTTGAA CCACAAGAAC AA
Details for NafoA.01385.a.B1.GE40623
| HARVESTED ON: | 10/18/2016 |
| SEQUENCED ON: | 10/19/2016 |
| EXPECTED MW: | 51kDa |
| OBSERVED MW: | 48kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Many (50-100) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMN EEYDVVVLGT GLTECVISGL LSVSGKKVLH MDRNPYYGGE SASLNLDQMF EKFRGQDAKP PASLGRSRDY NIDLIPKFLM ANGKLVKILR MTGVTRYNME FALVEGSFVY HKGEIHKVPI TPTEVAKTPL LGFFEKLKAK KLVSYLYDYD QNNPKTHQGF DCSKDTMDKI YKYYGVSEDT TDFLGHAVAL YTDDSYMTTV PALEVIERMR LYEDSLNMYG KSPYVYPMYG LGELPQVFAR LCAVYGGTYM LDKKVDRIVY DDNGHVVGVE SGGEVAKCKM VVGDPSYFPE KVRKTGKVIR VICILSHPVK STENAKSSQI IFPQKQTGRK HDIYCCVTSF THHVAPNGKY IAIVSTTVES DNPENEVKVV LDLLNPIDEK FVYILDTYAP LEDGKKDGVF ISKSYDATTH FESCAVDIVD IYERITGEKF DWNKKPVEPQ EQ
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaatg aagaatatga tgtggtcgtt ttaggtactg gtttaaccga atgtgtaatt tcaggtcttc tctccgtgag tggtaagaag gtcctccaca tggatcgtaa cccttactat ggaggtgaat ctgcttctct caacctggat caaatgtttg aaaagtttag aggacaagat gccaagcctc ctgcctctct cggtcgtagc agagattata atattgattt aattccaaaa ttcttgatgg ccaatggtaa actcgtaaag atcttgagaa tgactggtgt tactcgttat aatatggaat ttgctttggt ggaaggaagc tttgtctatc acaagggaga aatccacaag gtgccaatta ctccaaccga agttgctaaa acacccttgt tgggcttctt tgagaaattg aaggctaaaa agcttgtttc ctatttgtac gattatgatc aaaacaatcc aaagactcat caagggtttg attgttccaa agacacgatg gataagatct acaagtatta tggtgtgagt gaggacacta ctgacttttt gggacatgct gttgccttgt acacagatga ttcctacatg actactgttc ctgctcttga agttattgag agaatgagat tgtacgaaga ttcactcaat atgtacggaa agtctccata cgtttatcca atgtatggtt tgggtgaatt gccacaagtt tttgctcgtt tgtgtgctgt ctatggaggt acatacatgt tggataagaa ggttgatcga attgtctacg atgacaatgg tcatgttgtt ggtgttgaat cgggaggtga agttgctaaa tgtaagatgg ttgtcggaga tccaagctac ttccctgaga aggttcgcaa gactggaaag gttattcgtg tcatttgcat tctttctcat cctgtgaaga gcactgagaa tgccaagtct tcacaaatta ttttcccaca aaagcaaact ggaagaaagc atgatattta ttgttgtgtg acatcattca ctcaccacgt tgctccaaat ggcaaatata ttgccattgt ttctacaaca gttgagtcag acaatccaga gaacgaagtt aaagttgttc tcgatctttt gaacccaatt gatgagaagt ttgtttacat tctcgacact tatgctccat tagaggacgg aaagaaggat ggtgttttca tttctaaatc ctatgatgcc actactcact ttgaatcatg tgctgttgac attgttgata tctatgagag aattactggt gagaagtttg attggaataa gaagcctgtt gaaccacaag aacaatgagt aagataggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgaggcagct gcggtaaagc tcatcagcgt ggtcgtgaag cgattcacag atgtctgcct gttcatccgc gtccagctcg ttgagtttct ccagaagcgt taatgtctgg cttctgataa agcgggccat gttaagggcg gttttttcct gtttggtcac tgatgcctcc gtgtaagggg gatttctgtt catgggggta atgataccga tgaaacgaga gaggatgctc acgatacggg ttactgatga tgaacatgcc cggttactgg aacgttgtga gggtaaacaa ctggcggtat ggatgcggcg ggaccagaga aaaatcactc agggtcaatg ccagcgcttc gttaatacag atgtaggtgt tccacagggt agccagcagc atcctgcgat gcagatccgg aacataatgg tgcagggcgc tgacttccgc gtttccagac tttacgaaac acggaaaccg aagaccattc atgttgttgc tcaggtcgca gacgttttgc agcagcagtc gcttcacgtt cgctcgcgta tcggtgattc attctgctaa ccagtaaggc aaccccgcca gcctagccgg gtcctcaacg acaggagcac gatcatgcgc acccgtggcc aggacccaac gctgcccgag atgcgccgcg tgcggctgct ggagatggcg gacgcgatgg atatgttctg ccaagggttg gtttgcgcat tcacagttct ccgcaagaat tgattggctc caattcttgg agtggtgaat ccgttagcga ggtgccgccg gcttccattc aggtcgaggt ggcccggctc catgcaccgc gacgcaacgc ggggaggcag acaaggtata gggcggcgcc tacaatccat gccaacccgt tccatgtgct cgccgaggcg gcataaatcg ccgtgacgat cagcggtcca gtgatcgaag ttaggctggt aagagccgcg agcgatcctt gaagctgtcc ctgatggtcg tcatctacct gcctggacag catggcctgc aacgcgggca tcccgatgcc gccggaagcg agaagaatca taatggggaa ggccatccag cctcgcgtcg cgaacgccag caagacgtag cccagcgcgt cggccgccat gccggcgata atggcctgct tctcgccgaa acgtttggtg gcgggaccag tgacgaaggc ttgagcgagg gcgtgcaaga ttccgaatac cgcaagcgac aggccgatca tcgtcgcgct ccagcgaaag cggtcctcgc cgaaaatgac ccagagcgct gccggcacct gtcctacgag ttgcatgata aagaagacag tcataagtgc ggcgacgata gtcatgcccc gcgcccaccg gaaggagctg actgggttga aggctctcaa gggcatcggt cgacgctctc ccttatgcga ctcctgcatt aggaagcagc ccagtagtag gttgaggccg ttgagcaccg ccgccgcaag gaatggtgca tgcaaggaga tggcgcccaa cagtcccccg gccacggggc ctgccaccat acccacgccg aaacaagcgc tcatgagccc gaagtggcga gcccgatctt ccccatcggt gatgtcggcg atataggcgc cagcaaccgc acctgtggcg ccggtgatgc cggccacgat gcgtccggcg tagaggatcg agatctcgat cccgcgaaat
Details for NafoA.01385.a.B1.PS38253
| PURIFICATION DATe: | 4/6/2017 |
| CONCENTRATION: | 7.18mg/ml |
| OBSERVED MW: | 51kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | SEC: 20 mM HEPES pH 7.0, 300 mM NaCl, 5% glycerol, 1 mM TCEP |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| VIAL COUNT (approx.): | 5 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHMN EEYDVVVLGT GLTECVISGL LSVSGKKVLH MDRNPYYGGE SASLNLDQMF EKFRGQDAKP PASLGRSRDY NIDLIPKFLM ANGKLVKILR MTGVTRYNME FALVEGSFVY HKGEIHKVPI TPTEVAKTPL LGFFEKLKAK KLVSYLYDYD QNNPKTHQGF DCSKDTMDKI YKYYGVSEDT TDFLGHAVAL YTDDSYMTTV PALEVIERMR LYEDSLNMYG KSPYVYPMYG LGELPQVFAR LCAVYGGTYM LDKKVDRIVY DDNGHVVGVE SGGEVAKCKM VVGDPSYFPE KVRKTGKVIR VICILSHPVK STENAKSSQI IFPQKQTGRK HDIYCCVTSF THHVAPNGKY IAIVSTTVES DNPENEVKVV LDLLNPIDEK FVYILDTYAP LEDGKKDGVF ISKSYDATTH FESCAVDIVD IYERITGEKF DWNKKPVEPQ EQ
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAATG AAGAATATGA TGTGGTCGTT TTAGGTACTG GTTTAACCGA ATGTGTAATT TCAGGTCTTC TCTCCGTGAG TGGTAAGAAG GTCCTCCACA TGGATCGTAA CCCTTACTAT GGAGGTGAAT CTGCTTCTCT CAACCTGGAT CAAATGTTTG AAAAGTTTAG AGGACAAGAT GCCAAGCCTC CTGCCTCTCT CGGTCGTAGC AGAGATTATA ATATTGATTT AATTCCAAAA TTCTTGATGG CCAATGGTAA ACTCGTAAAG ATCTTGAGAA TGACTGGTGT TACTCGTTAT AATATGGAAT TTGCTTTGGT GGAAGGAAGC TTTGTCTATC ACAAGGGAGA AATCCACAAG GTGCCAATTA CTCCAACCGA AGTTGCTAAA ACACCCTTGT TGGGCTTCTT TGAGAAATTG AAGGCTAAAA AGCTTGTTTC CTATTTGTAC GATTATGATC AAAACAATCC AAAGACTCAT CAAGGGTTTG ATTGTTCCAA AGACACGATG GATAAGATCT ACAAGTATTA TGGTGTGAGT GAGGACACTA CTGACTTTTT GGGACATGCT GTTGCCTTGT ACACAGATGA TTCCTACATG ACTACTGTTC CTGCTCTTGA AGTTATTGAG AGAATGAGAT TGTACGAAGA TTCACTCAAT ATGTACGGAA AGTCTCCATA CGTTTATCCA ATGTATGGTT TGGGTGAATT GCCACAAGTT TTTGCTCGTT TGTGTGCTGT CTATGGAGGT ACATACATGT TGGATAAGAA GGTTGATCGA ATTGTCTACG ATGACAATGG TCATGTTGTT GGTGTTGAAT CGGGAGGTGA AGTTGCTAAA TGTAAGATGG TTGTCGGAGA TCCAAGCTAC TTCCCTGAGA AGGTTCGCAA GACTGGAAAG GTTATTCGTG TCATTTGCAT TCTTTCTCAT CCTGTGAAGA GCACTGAGAA TGCCAAGTCT TCACAAATTA TTTTCCCACA AAAGCAAACT GGAAGAAAGC ATGATATTTA TTGTTGTGTG ACATCATTCA CTCACCACGT TGCTCCAAAT GGCAAATATA TTGCCATTGT TTCTACAACA GTTGAGTCAG ACAATCCAGA GAACGAAGTT AAAGTTGTTC TCGATCTTTT GAACCCAATT GATGAGAAGT TTGTTTACAT TCTCGACACT TATGCTCCAT TAGAGGACGG AAAGAAGGAT GGTGTTTTCA TTTCTAAATC CTATGATGCC ACTACTCACT TTGAATCATG TGCTGTTGAC ATTGTTGATA TCTATGAGAG AATTACTGGT GAGAAGTTTG ATTGGAATAA GAAGCCTGTT GAACCACAAG AACAATGAGT AAGATAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA ATTCTTGAAG ACGAAAGGGC CTCGTGATAC GCCTATTTTT ATAGGTTAAT GTCATGATAA TAATGGTTTC TTAGACGTCA GGTGGCACTT TTCGGGGAAA TGTGCGCGGA ACCCCTATTT GTTTATTTTT CTAAATACAT TCAAATATGT ATCCGCTCAT GAGACAATAA CCCTGATAAA TGCTTCAATA ATATTGAAAA AGGAAGAGTA TGAGTATTCA ACATTTCCGT GTCGCCCTTA TTCCCTTTTT TGCGGCATTT TGCCTTCCTG TTTTTGCTCA CCCAGAAACG CTGGTGAAAG TAAAAGATGC TGAAGATCAG TTGGGTGCAC GAGTGGGTTA CATCGAACTG GATCTCAACA GCGGTAAGAT CCTTGAGAGT TTTCGCCCCG AAGAACGTTT TCCAATGATG AGCACTTTTA AAGTTCTGCT ATGTGGCGCG GTATTATCCC GTGTTGACGC CGGGCAAGAG CAACTCGGTC GCCGCATACA CTATTCTCAG AATGACTTGG TTGAGTACTC ACCAGTCACA GAAAAGCATC TTACGGATGG CATGACAGTA AGAGAATTAT GCAGTGCTGC CATAACCATG AGTGATAACA CTGCGGCCAA CTTACTTCTG ACAACGATCG GAGGACCGAA GGAGCTAACC GCTTTTTTGC ACAACATGGG GGATCATGTA ACTCGCCTTG ATCGTTGGGA ACCGGAGCTG AATGAAGCCA TACCAAACGA CGAGCGTGAC ACCACGATGC CTGCAGCAAT GGCAACAACG TTGCGCAAAC TATTAACTGG CGAACTACTT ACTCTAGCTT CCCGGCAACA ATTAATAGAC TGGATGGAGG CGGATAAAGT TGCAGGACCA CTTCTGCGCT CGGCCCTTCC GGCTGGCTGG TTTATTGCTG ATAAATCTGG AGCCGGTGAG CGTGGGTCTC GCGGTATCAT TGCAGCACTG GGGCCAGATG GTAAGCCCTC CCGTATCGTA GTTATCTACA CGACGGGGAG TCAGGCAACT ATGGATGAAC GAAATAGACA GATCGCTGAG ATAGGTGCCT CACTGATTAA GCATTGGTAA CTGTCAGACC AAGTTTACTC ATATATACTT TAGATTGATT TAAAACTTCA TTTTTAATTT AAAAGGATCT AGGTGAAGAT CCTTTTTGAT AATCTCATGA CCAAAATCCC TTAACGTGAG TTTTCGTTCC ACTGAGCGTC AGACCCCGTA GAAAAGATCA AAGGATCTTC TTGAGATCCT TTTTTTCTGC GCGTAATCTG CTGCTTGCAA ACAAAAAAAC CACCGCTACC AGCGGTGGTT TGTTTGCCGG ATCAAGAGCT ACCAACTCTT TTTCCGAAGG TAACTGGCTT CAGCAGAGCG CAGATACCAA ATACTGTCCT TCTAGTGTAG CCGTAGTTAG GCCACCACTT CAAGAACTCT GTAGCACCGC CTACATACCT CGCTCTGCTA ATCCTGTTAC CAGTGGCTGC TGCCAGTGGC GATAAGTCGT GTCTTACCGG GTTGGACTCA AGACGATAGT TACCGGATAA GGCGCAGCGG TCGGGCTGAA CGGGGGGTTC GTGCACACAG CCCAGCTTGG AGCGAACGAC CTACACCGAA CTGAGATACC TACAGCGTGA GCTATGAGAA AGCGCCACGC TTCCCGAAGG GAGAAAGGCG GACAGGTATC CGGTAAGCGG CAGGGTCGGA ACAGGAGAGC GCACGAGGGA GCTTCCAGGG GGAAACGCCT GGTATCTTTA TAGTCCTGTC GGGTTTCGCC ACCTCTGACT TGAGCGTCGA TTTTTGTGAT GCTCGTCAGG GGGGCGGAGC CTATGGAAAA ACGCCAGCAA CGCGGCCTTT TTACGGTTCC TGGCCTTTTG CTGGCCTTTT GCTCACATGT TCTTTCCTGC GTTATCCCCT GATTCTGTGG ATAACCGTAT TACCGCCTTT GAGTGAGCTG ATACCGCTCG CCGCAGCCGA ACGACCGAGC GCAGCGAGTC AGTGAGCGAG GAAGCGGAAG AGCGCCTGAT GCGGTATTTT CTCCTTACGC ATCTGTGCGG TATTTCACAC CGCATATATG GTGCACTCTC AGTACAATCT GCTCTGATGC CGCATAGTTA AGCCAGTATA CACTCCGCTA TCGCTACGTG ACTGGGTCAT GGCTGCGCCC CGACACCCGC CAACACCCGC TGACGCGCCC TGACGGGCTT GTCTGCTCCC GGCATCCGCT TACAGACAAG CTGTGACCGT CTCCGGGAGC TGCATGTGTC AGAGGTTTTC ACCGTCATCA CCGAAACGCG CGAGGCAGCT GCGGTAAAGC TCATCAGCGT GGTCGTGAAG CGATTCACAG ATGTCTGCCT GTTCATCCGC GTCCAGCTCG TTGAGTTTCT CCAGAAGCGT TAATGTCTGG CTTCTGATAA AGCGGGCCAT GTTAAGGGCG GTTTTTTCCT GTTTGGTCAC TGATGCCTCC GTGTAAGGGG GATTTCTGTT CATGGGGGTA ATGATACCGA TGAAACGAGA GAGGATGCTC ACGATACGGG TTACTGATGA TGAACATGCC CGGTTACTGG AACGTTGTGA GGGTAAACAA CTGGCGGTAT GGATGCGGCG GGACCAGAGA AAAATCACTC AGGGTCAATG CCAGCGCTTC GTTAATACAG ATGTAGGTGT TCCACAGGGT AGCCAGCAGC ATCCTGCGAT GCAGATCCGG AACATAATGG TGCAGGGCGC TGACTTCCGC GTTTCCAGAC TTTACGAAAC ACGGAAACCG AAGACCATTC ATGTTGTTGC TCAGGTCGCA GACGTTTTGC AGCAGCAGTC GCTTCACGTT CGCTCGCGTA TCGGTGATTC ATTCTGCTAA CCAGTAAGGC AACCCCGCCA GCCTAGCCGG GTCCTCAACG ACAGGAGCAC GATCATGCGC ACCCGTGGCC AGGACCCAAC GCTGCCCGAG ATGCGCCGCG TGCGGCTGCT GGAGATGGCG GACGCGATGG ATATGTTCTG CCAAGGGTTG GTTTGCGCAT TCACAGTTCT CCGCAAGAAT TGATTGGCTC CAATTCTTGG AGTGGTGAAT CCGTTAGCGA GGTGCCGCCG GCTTCCATTC AGGTCGAGGT GGCCCGGCTC CATGCACCGC GACGCAACGC GGGGAGGCAG ACAAGGTATA GGGCGGCGCC TACAATCCAT GCCAACCCGT TCCATGTGCT CGCCGAGGCG GCATAAATCG CCGTGACGAT CAGCGGTCCA GTGATCGAAG TTAGGCTGGT AAGAGCCGCG AGCGATCCTT GAAGCTGTCC CTGATGGTCG TCATCTACCT GCCTGGACAG CATGGCCTGC AACGCGGGCA TCCCGATGCC GCCGGAAGCG AGAAGAATCA TAATGGGGAA GGCCATCCAG CCTCGCGTCG CGAACGCCAG CAAGACGTAG CCCAGCGCGT CGGCCGCCAT GCCGGCGATA ATGGCCTGCT TCTCGCCGAA ACGTTTGGTG GCGGGACCAG TGACGAAGGC TTGAGCGAGG GCGTGCAAGA TTCCGAATAC CGCAAGCGAC AGGCCGATCA TCGTCGCGCT CCAGCGAAAG CGGTCCTCGC CGAAAATGAC CCAGAGCGCT GCCGGCACCT GTCCTACGAG TTGCATGATA AAGAAGACAG TCATAAGTGC GGCGACGATA GTCATGCCCC GCGCCCACCG GAAGGAGCTG ACTGGGTTGA AGGCTCTCAA GGGCATCGGT CGACGCTCTC CCTTATGCGA CTCCTGCATT AGGAAGCAGC CCAGTAGTAG GTTGAGGCCG TTGAGCACCG CCGCCGCAAG GAATGGTGCA TGCAAGGAGA TGGCGCCCAA CAGTCCCCCG GCCACGGGGC CTGCCACCAT ACCCACGCCG AAACAAGCGC TCATGAGCCC GAAGTGGCGA GCCCGATCTT CCCCATCGGT GATGTCGGCG ATATAGGCGC CAGCAACCGC ACCTGTGGCG CCGGTGATGC CGGCCACGAT GCGTCCGGCG TAGAGGATCG AGATCTCGAT CCCGCGAAAT