NafoA.01238.a
Serine--tRNA ligase
| CENTER ID: | NafoA.01238.a |
| ORGANISM: | Naegleria fowleri ATCC 30863 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIB |
Ordering Clones & Proteins
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| NafoA.01238.a.B1.GE40480 | Full length(NafoA.01238.a) | 1 | 477 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| NafoA.01238.a.B1.PW38511 | Full length(NafoA.01238.a) | 1 | 477 |
Structures
6BLJ
DEPOSITED: 11/10/2017
DETERMINATION: XRay
CLONE: NafoA.01238.a.B1.GE40480
PROTEIN: NafoA.01238.a.B1.PW37973
Publications by SSGCID
Naegleria fowleri: Protein structures to facilitate drug discovery for the deadly, pathogenic free-living amoeba
Abendroth J, Barrett LK, Dranow DM, Edwards TE, Myler PJ, Phan IQ, Staker B, Subramanian S, Tran N, Van Voorhis WC, Craig JC, Smith C, Chun IKH, Kyle D, Barrett KF, Morris JC, Tillery L, Rice CA, Goldstein J, Lassner JW, Xu L, Young RM, Osterhout B, Leahy JW, Manetsch R
PLoS ONE - 2021
volume 16, issue 3, pages e0241738
PMID: 33760815; PMCID: PMC7990177
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| EuPathDB: | AmoebaDB:NF0119870 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLDINLFREY KGGNPEIIRE SQRRRFADVT LVDKVIELDE VWRATIGKLN HIKSFTGIIS KEVGNRMKNK VPLGDDLELP KEVTDDVYAL FTKEALEQGS LAKLNTNQLK KLSTYITEVH IKNSEEEVKQ KEKERDDVLL QIGNIVHETV VVSDNEDNNG IVRMVGNPRP KVDPETGYKC LKHIDIMRKL GGLATEEGTQ VGGGRGYFLL GDLVRMNLAL QNYAIDFLAK KGYMPIYTPF FMTKEQMKKV AQLSQFDEEL YTVTGEGEDK YLIATSEQPI AAFHLEKRFD ESELPIKYCG MSTCFRKEVG AHGKDTLGIF RVHQFEKIEQ FVVTSPKDNK SWEMFDEMIG NSEAFYQSLG IPYRVVNIVS GALNNAAAKK FDLEAWFPGA DEGNEYRELV SCSNCTDYQT RRLEVKYGKS KKQGSEVEFC HMLNSTLTAT SRTLCCIVEN YQTPEGVNVP EVLQPYMGGT KFIKFKN
NT Sequence
ATGCTCGATA TTAATTTATT CAGAGAATAC AAGGGCGGTA ATCCAGAGAT TATTAGAGAG TCTCAACGAA GAAGATTTGC CGATGTTACT CTTGTTGATA AAGTCATTGA ATTGGACGAA GTATGGAGAG CTACCATTGG TAAATTGAAT CATATCAAGA GTTTTACTGG TATTATCAGC AAGGAAGTTG GTAATCGTAT GAAAAACAAG GTTCCACTCG GTGATGATTT GGAATTACCA AAAGAAGTCA CTGATGATGT TTATGCCCTC TTCACAAAGG AGGCCCTCGA ACAAGGATCT TTGGCAAAAC TCAATACCAA TCAACTGAAA AAGCTCTCTA CATACATTAC CGAAGTTCAT ATTAAGAATT CTGAAGAGGA GGTGAAGCAA AAAGAAAAGG AGAGAGATGA TGTTTTACTT CAAATTGGTA ATATTGTTCA TGAGACTGTT GTTGTCAGTG ATAATGAAGA TAATAATGGT ATTGTGAGAA TGGTCGGTAA TCCACGACCA AAAGTTGATC CAGAGACTGG ATACAAATGT CTCAAGCATA TTGATATCAT GAGAAAATTA GGAGGATTAG CCACAGAAGA AGGTACTCAA GTCGGTGGTG GAAGAGGTTA CTTTTTACTT GGAGATTTGG TCAGAATGAA TTTGGCTCTT CAAAATTATG CCATTGACTT TTTGGCCAAG AAGGGATACA TGCCAATTTA CACACCATTC TTCATGACTA AGGAACAAAT GAAGAAGGTA GCTCAATTGT CTCAATTTGA TGAAGAATTA TATACTGTCA CAGGAGAAGG TGAAGACAAG TATTTGATTG CCACCTCTGA ACAACCAATT GCTGCTTTCC ACTTGGAGAA GCGTTTTGAT GAGAGTGAGC TTCCAATCAA GTACTGCGGT ATGTCCACAT GTTTCAGAAA AGAAGTTGGT GCTCACGGTA AGGACACCTT GGGTATTTTC CGTGTTCACC AATTCGAAAA GATTGAACAA TTTGTTGTCA CTTCTCCAAA GGACAATAAG AGTTGGGAAA TGTTCGATGA AATGATTGGA AACTCTGAAG CTTTCTATCA AAGTCTTGGA ATTCCATACA GAGTGGTAAA TATTGTCAGC GGTGCTTTGA ATAATGCTGC TGCCAAGAAA TTCGACTTGG AAGCTTGGTT CCCAGGAGCT GATGAAGGTA ACGAATACAG AGAATTGGTT TCTTGCTCCA ACTGTACCGA CTACCAAACT AGAAGACTCG AGGTCAAGTA TGGTAAGAGC AAGAAGCAAG GTAGTGAGGT TGAATTTTGC CACATGTTGA ACTCTACATT GACTGCTACC TCTAGAACAC TTTGTTGTAT CGTTGAAAAC TATCAAACAC CAGAAGGAGT GAATGTTCCT GAAGTTTTGC AACCTTACAT GGGTGGCACT AAATTCATCA AGTTCAAGAA T
Details for NafoA.01238.a.B1.GE40480
| HARVESTED ON: | 8/10/2016 |
| SEQUENCED ON: | 8/11/2016 |
| EXPECTED MW: | 55kDa |
| OBSERVED MW: | 55kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHML DINLFREYKG GNPEIIRESQ RRRFADVTLV DKVIELDEVW RATIGKLNHI KSFTGIISKE VGNRMKNKVP LGDDLELPKE VTDDVYALFT KEALEQGSLA KLNTNQLKKL STYITEVHIK NSEEEVKQKE KERDDVLLQI GNIVHETVVV SDNEDNNGIV RMVGNPRPKV DPETGYKCLK HIDIMRKLGG LATEEGTQVG GGRGYFLLGD LVRMNLALQN YAIDFLAKKG YMPIYTPFFM TKEQMKKVAQ LSQFDEELYT VTGEGEDKYL IATSEQPIAA FHLEKRFDES ELPIKYCGMS TCFRKEVGAH GKDTLGIFRV HQFEKIEQFV VTSPKDNKSW EMFDEMIGNS EAFYQSLGIP YRVVNIVSGA LNNAAAKKFD LEAWFPGADE GNEYRELVSC SNCTDYQTRR LEVKYGKSKK QGSEVEFCHM LNSTLTATSR TLCCIVENYQ TPEGVNVPEV LQPYMGGTKF IKFKN
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgctcg atattaattt attcagagaa tacaagggcg gtaatccaga gattattaga gagtctcaac gaagaagatt tgccgatgtt actcttgttg ataaagtcat tgaattggac gaagtatgga gagctaccat tggtaaattg aatcatatca agagttttac tggtattatc agcaaggaag ttggtaatcg tatgaaaaac aaggttccac tcggtgatga tttggaatta ccaaaagaag tcactgatga tgtttatgcc ctcttcacaa aggaggccct cgaacaagga tctttggcaa aactcaatac caatcaactg aaaaagctct ctacatacat taccgaagtt catattaaga attctgaaga ggaggtgaag caaaaagaaa aggagagaga tgatgtttta cttcaaattg gtaatattgt tcatgagact gttgttgtca gtgataatga agataataat ggtattgtga gaatggtcgg taatccacga ccaaaagttg atccagagac tggatacaaa tgtctcaagc atattgatat catgagaaaa ttaggaggat tagccacaga agaaggtact caagtcggtg gtggaagagg ttacttttta cttggagatt tggtcagaat gaatttggct cttcaaaatt atgccattga ctttttggcc aagaagggat acatgccaat ttacacacca ttcttcatga ctaaggaaca aatgaagaag gtagctcaat tgtctcaatt tgatgaagaa ttatatactg tcacaggaga aggtgaagac aagtatttga ttgccacctc tgaacaacca attgctgctt tccacttgga gaagcgtttt gatgagagtg agcttccaat caagtactgc ggtatgtcca catgtttcag aaaagaagtt ggtgctcacg gtaaggacac cttgggtatt ttccgtgttc accaattcga aaagattgaa caatttgttg tcacttctcc aaaggacaat aagagttggg aaatgttcga tgaaatgatt ggaaactctg aagctttcta tcaaagtctt ggaattccat acagagtggt aaatattgtc agcggtgctt tgaataatgc tgctgccaag aaattcgact tggaagcttg gttcccagga gctgatgaag gtaacgaata cagagaattg gtttcttgct ccaactgtac cgactaccaa actagaagac tcgaggtcaa gtatggtaag agcaagaagc aaggtagtga ggttgaattt tgccacatgt tgaactctac attgactgct acctctagaa cactttgttg tatcgttgaa aactatcaaa caccagaagg agtgaatgtt cctgaagttt tgcaacctta catgggtggc actaaattca tcaagttcaa gaattgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat
Details for NafoA.01238.a.B1.PW38511
| PURIFICATION DATe: | 7/23/2018 |
| CONCENTRATION: | 10.7mg/ml |
| OBSERVED MW: | 55kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7, 500 mM NaCl, 5% Glycerol , 2 mM DTT, 0.025% Azide |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| VIAL COUNT (approx.): | 8 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHML DINLFREYKG GNPEIIRESQ RRRFADVTLV DKVIELDEVW RATIGKLNHI KSFTGIISKE VGNRMKNKVP LGDDLELPKE VTDDVYALFT KEALEQGSLA KLNTNQLKKL STYITEVHIK NSEEEVKQKE KERDDVLLQI GNIVHETVVV SDNEDNNGIV RMVGNPRPKV DPETGYKCLK HIDIMRKLGG LATEEGTQVG GGRGYFLLGD LVRMNLALQN YAIDFLAKKG YMPIYTPFFM TKEQMKKVAQ LSQFDEELYT VTGEGEDKYL IATSEQPIAA FHLEKRFDES ELPIKYCGMS TCFRKEVGAH GKDTLGIFRV HQFEKIEQFV VTSPKDNKSW EMFDEMIGNS EAFYQSLGIP YRVVNIVSGA LNNAAAKKFD LEAWFPGADE GNEYRELVSC SNCTDYQTRR LEVKYGKSKK QGSEVEFCHM LNSTLTATSR TLCCIVENYQ TPEGVNVPEV LQPYMGGTKF IKFKN
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGCTCG ATATTAATTT ATTCAGAGAA TACAAGGGCG GTAATCCAGA GATTATTAGA GAGTCTCAAC GAAGAAGATT TGCCGATGTT ACTCTTGTTG ATAAAGTCAT TGAATTGGAC GAAGTATGGA GAGCTACCAT TGGTAAATTG AATCATATCA AGAGTTTTAC TGGTATTATC AGCAAGGAAG TTGGTAATCG TATGAAAAAC AAGGTTCCAC TCGGTGATGA TTTGGAATTA CCAAAAGAAG TCACTGATGA TGTTTATGCC CTCTTCACAA AGGAGGCCCT CGAACAAGGA TCTTTGGCAA AACTCAATAC CAATCAACTG AAAAAGCTCT CTACATACAT TACCGAAGTT CATATTAAGA ATTCTGAAGA GGAGGTGAAG CAAAAAGAAA AGGAGAGAGA TGATGTTTTA CTTCAAATTG GTAATATTGT TCATGAGACT GTTGTTGTCA GTGATAATGA AGATAATAAT GGTATTGTGA GAATGGTCGG TAATCCACGA CCAAAAGTTG ATCCAGAGAC TGGATACAAA TGTCTCAAGC ATATTGATAT CATGAGAAAA TTAGGAGGAT TAGCCACAGA AGAAGGTACT CAAGTCGGTG GTGGAAGAGG TTACTTTTTA CTTGGAGATT TGGTCAGAAT GAATTTGGCT CTTCAAAATT ATGCCATTGA CTTTTTGGCC AAGAAGGGAT ACATGCCAAT TTACACACCA TTCTTCATGA CTAAGGAACA AATGAAGAAG GTAGCTCAAT TGTCTCAATT TGATGAAGAA TTATATACTG TCACAGGAGA AGGTGAAGAC AAGTATTTGA TTGCCACCTC TGAACAACCA ATTGCTGCTT TCCACTTGGA GAAGCGTTTT GATGAGAGTG AGCTTCCAAT CAAGTACTGC GGTATGTCCA CATGTTTCAG AAAAGAAGTT GGTGCTCACG GTAAGGACAC CTTGGGTATT TTCCGTGTTC ACCAATTCGA AAAGATTGAA CAATTTGTTG TCACTTCTCC AAAGGACAAT AAGAGTTGGG AAATGTTCGA TGAAATGATT GGAAACTCTG AAGCTTTCTA TCAAAGTCTT GGAATTCCAT ACAGAGTGGT AAATATTGTC AGCGGTGCTT TGAATAATGC TGCTGCCAAG AAATTCGACT TGGAAGCTTG GTTCCCAGGA GCTGATGAAG GTAACGAATA CAGAGAATTG GTTTCTTGCT CCAACTGTAC CGACTACCAA ACTAGAAGAC TCGAGGTCAA GTATGGTAAG AGCAAGAAGC AAGGTAGTGA GGTTGAATTT TGCCACATGT TGAACTCTAC ATTGACTGCT ACCTCTAGAA CACTTTGTTG TATCGTTGAA AACTATCAAA CACCAGAAGG AGTGAATGTT CCTGAAGTTT TGCAACCTTA CATGGGTGGC ACTAAATTCA TCAAGTTCAA GAATTGAGTA AGATAGGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATATCCACAG GACGGGTGTG GTCGCCATGA TCGCGTAGTC GATAGTGGCT CCAAGTAGCG AAGCGAGCAG GACTGGGCGG CGGCCAAAGC GGTCGGACAG TGCTCCGAGA ACGGGTGCGC ATAGAAATTG CATCAACGCA TATAGCGCTA GCAGCACGCC ATAGTGACTG GCGATGCTGT CGGAATGGAC GATATCCCGC AAGAGGCCCG GCAGTACCGG CATAACCAAG CCTATGCCTA CAGCATCCAG GGTGACGGTG CCGAGGATGA CGATGAGCGC ATTGTTAGAT TTCATACACG GTGCCTGACT GCGTTAGCAA TTTAACTGTG ATAAACTACC GCATTAAAGC TTATCGATGA TAAGCTGTCA AACATGAGAA TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGC GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGCCATG CCGGCGATAA TGGCCTGCTT CTCGCCGAAA CGTTTGGTGG CGGGACCAGT GACGAAGGCT TGAGCGAGGG CGTGCAAGAT TCCGAATACC GCAAGCGACA GGCCGATCAT CGTCGCGCTC CAGCGAAAGC GGTCCTCGCC GAAAATGACC CAGAGCGCTG CCGGCACCTG TCCTACGAGT TGCATGATAA AGAAGACAGT CATAAGTGCG GCGACGATAG TCATGCCCCG CGCCCACCGG AAGGAGCTGA CTGGGTTGAA GGCTCTCAAG GGCATCGGTC GACGCTCTCC CTTATGCGAC TCCTGCATTA GGAAGCAGCC CAGTAGTAGG TTGAGGCCGT TGAGCACCGC CGCCGCAAGG AATGGTGCAT GCAAGGAGAT GGCGCCCAAC AGTCCCCCGG CCACGGGGCC TGCCACCATA CCCACGCCGA AACAAGCGCT CATGAGCCCG AAGTGGCGAG CCCGATCTTC CCCATCGGTG ATGTCGGCGA TATAGGCGCC AGCAACCGCA CCTGTGGCGC CGGTGATGCC GGCCACGATG CGTCCGGCGT AGAGGATCGA GATCTCGATC CCGCGAAAT