NafoA.01145.a

26s proteasome regulatory subunit 4 homolog b-like,regulatory particle aaa-atpase 2a isoform 2

CENTER ID:	NafoA.01145.a
ORGANISM:	Naegleria fowleri ATCC 30863
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
NafoA.01145.a.B2.GE40619	D1-D2 nucleotide binding domains		54	453

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	AmoebaDB:NF0052280

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MGNVPSGGQN PFNQLPGAGS QKKKDDKKKK KEKKKFEQPT PTRIGRKKRK KGPQIAEKLP EVTPLSKCKL RLLRLERIQD YLTMEKEFVT NQAKLLSAQE KSKDMEQERN MVDELRGSPM AVGTLEEIID DNHAIVSSSV GPEFYVSILS IVDKDQLEPG CSVLMHNRYL AVVGILQDEA DPMVSVMKVD KAPLESYSQI GGLEEQIQEI KEAVEFPLTH PELYEDIGIK PPKGVILYGE PGTGKTLLAK AVAHHTSATF LRVVGSELIQ KYLGEGPKLV RELFRVAEEL APSIVFIDEI DAIGTKRFDS TSGGEREIQR TMLELLNQLD GFDSRGDVKV IMATNRIESL DTALIRPGRI DRKIKFPFPD IKTKRKIFEI HTRKMNKAAD VNLDEFISSK DELSGADIKA VCTEAGLLAL RERRLQVTQD DFTKAKEKVL YKKQQGIPEG LYI

NT Sequence

ATGGGTAACG TACCAAGCGG AGGACAAAAT CCGTTCAATC AACTGCCAGG AGCAGGTAGT CAAAAGAAAA AGGACGATAA GAAGAAAAAG AAGGAAAAGA AAAAGTTTGA GCAGCCAACA CCAACCCGTA TTGGAAGAAA GAAGAGGAAA AAGGGTCCAC AAATTGCAGA GAAATTACCT GAAGTTACTC CTTTGTCAAA ATGTAAACTT AGATTATTGC GATTGGAAAG AATACAAGAT TATCTTACAA TGGAGAAGGA ATTTGTAACC AATCAAGCCA AACTTTTATC TGCACAAGAA AAATCCAAGG ACATGGAACA AGAACGTAAC ATGGTGGATG AATTGAGAGG ATCTCCAATG GCTGTCGGAA CTCTCGAAGA AATTATTGAC GACAATCATG CCATTGTATC GAGCAGTGTT GGCCCTGAAT TTTACGTTTC AATCCTTTCG ATTGTAGACA AGGATCAGTT GGAACCAGGA TGCAGTGTCT TGATGCACAA TAGATACCTT GCCGTTGTTG GTATTCTTCA AGATGAAGCC GATCCAATGG TCTCTGTTAT GAAAGTCGAT AAGGCTCCAC TTGAATCATA TTCACAAATC GGAGGTTTGG AAGAGCAAAT TCAAGAAATT AAGGAAGCTG TCGAGTTCCC ATTGACTCAC CCTGAACTTT ATGAGGATAT TGGTATTAAA CCACCAAAGG GAGTGATTCT TTATGGAGAA CCAGGTACAG GAAAGACATT ACTTGCAAAA GCTGTTGCTC ACCACACTAG TGCTACATTC TTGCGTGTGG TTGGATCAGA GTTGATTCAA AAGTATCTTG GAGAGGGTCC TAAACTCGTT CGTGAACTGT TCAGAGTTGC AGAAGAATTA GCACCAAGTA TTGTATTTAT TGACGAAATT GATGCTATCG GTACAAAGAG ATTTGACTCT ACTTCTGGAG GTGAACGTGA GATTCAAAGA ACTATGCTTG AATTATTGAA TCAATTGGAT GGTTTTGATT CAAGAGGAGA TGTCAAAGTA ATTATGGCTA CAAATAGAAT TGAGTCTTTG GATACAGCCT TGATCAGACC AGGTAGAATT GACCGTAAGA TAAAGTTCCC ATTCCCTGAC ATTAAGACAA AGAGAAAGAT CTTTGAAATT CATACACGTA AGATGAACAA GGCTGCAGAT GTAAATTTGG ATGAATTTAT CTCATCAAAG GATGAACTTT CTGGTGCTGA CATTAAGGCT GTGTGCACAG AAGCAGGTTT ACTTGCTCTC AGAGAACGTA GACTTCAAGT GACACAAGAC GACTTCACCA AAGCCAAAGA AAAGGTGCTG TATAAAAAGC AACAAGGCAT TCCAGAAGGT CTCTACATT

Details for NafoA.01145.a.B2.GE40619

HARVESTED ON:	10/18/2016
SEQUENCED ON:	10/19/2016
EXPECTED MW:	46kDa
OBSERVED MW:	46kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	98

Validated AA Sequence

MAHHHHHHMQ IAEKLPEVTP LSKCKLRLLR LERIQDYLTM EKEFVTNQAK LLSAQEKSKD MEQERNMVDE LRGSPMAVGT LEEIIDDNHA IVSSSVGPEF YVSILSIVDK DQLEPGCSVL MHNRYLAVVG ILQDEADPMV SVMKVDKAPL ESYSQIGGLE EQIQEIKEAV EFPLTHPELY EDIGIKPPKG VILYGEPGTG KTLLAKAVAH HTSATFLRVV GSELIQKYLG EGPKLVRELF RVAEELAPSI VFIDEIDAIG TKRFDSTSGG EREIQRTMLE LLNQLDGFDS RGDVKVIMAT NRIESLDTAL IRPGRIDRKI KFPFPDIKTK RKIFEIHTRK MNKAADVNLD EFISSKDELS GADIKAVCTE AGLLALRERR LQVTQDDFTK AKEKVLYKKQ

Validated NT Sequence

ttgttgcttt ttatacagca ccttttcttt ggctttggtg aagtcgtctt gtgtcacttg aagtctacgt tctctgagag caagtaaacc tgcttctgtg cacacagcct taatgtcagc accagaaagt tcatcctttg atgagataaa ttcatccaaa tttacatctg cagccttgtt catcttacgt gtatgaattt caaagatctt tctctttgtc ttaatgtcag ggaatgggaa ctttatctta cggtcaattc tacctggtct gatcaaggct gtatccaaag actcaattct atttgtagcc ataattactt tgacatctcc tcttgaatca aaaccatcca attgattcaa taattcaagc atagttcttt gaatctcacg ttcacctcca gaagtagagt caaatctctt tgtaccgata gcatcaattt cgtcaataaa tacaatactt ggtgctaatt cttctgcaac tctgaacagt tcacgaacga gtttaggacc ctctccaaga tacttttgaa tcaactctga tccaaccaca cgcaagaatg tagcactagt gtggtgagca acagcttttg caagtaatgt ctttcctgta cctggttctc cataaagaat cactcccttt ggtggtttaa taccaatatc ctcataaagt tcagggtgag tcaatgggaa ctcgacagct tccttaattt cttgaatttg ctcttccaaa cctccgattt gtgaatatga ttcaagagga gccttatcga ctttcataac agagaccatt ggatcggctt catcttgaag aataccaaca acggcaaggt atctattgtg catcaagaca ctgcatcctg gttccaactg atccttgtct acaatcgaaa ggattgaaac gtaaaattca gggccaacac tgctcgatac aatggcatga ttgtcgtcaa taatttcttc gagagttccg acagccattg gagatcctct caattcatcc accatgttac gttcttgttc catgtccttg gatttttctt gtgcagataa aagtttggct tgattggtta caaattcctt ctccattgta agataatctt gtattctttc caatcgcaat aatctaagtt tacattttga caaaggagta acttcaggta atttctctgc aatttgcata tggtggtggt ggtggtgagc cat

Expected Protein Sequence

MAHHHHHHQI AEKLPEVTPL SKCKLRLLRL ERIQDYLTME KEFVTNQAKL LSAQEKSKDM EQERNMVDEL RGSPMAVGTL EEIIDDNHAI VSSSVGPEFY VSILSIVDKD QLEPGCSVLM HNRYLAVVGI LQDEADPMVS VMKVDKAPLE SYSQIGGLEE QIQEIKEAVE FPLTHPELYE DIGIKPPKGV ILYGEPGTGK TLLAKAVAHH TSATFLRVVG SELIQKYLGE GPKLVRELFR VAEELAPSIV FIDEIDAIGT KRFDSTSGGE REIQRTMLEL LNQLDGFDSR GDVKVIMATN RIESLDTALI RPGRIDRKIK FPFPDIKTKR KIFEIHTRKM NKAADVNLDE FISSKDELSG ADIKAVCTEA GLLALRERRL QVTQDDFTKA KEKVLYKKQQ GIPEGLYI

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catcaaattg cagagaaatt acctgaagtt actcctttgt caaaatgtaa acttagatta ttgcgattgg aaagaataca agattatctt acaatggaga aggaatttgt aaccaatcaa gccaaacttt tatctgcaca agaaaaatcc aaggacatgg aacaagaacg taacatggtg gatgaattga gaggatctcc aatggctgtc ggaactctcg aagaaattat tgacgacaat catgccattg tatcgagcag tgttggccct gaattttacg tttcaatcct ttcgattgta gacaaggatc agttggaacc aggatgcagt gtcttgatgc acaatagata ccttgccgtt gttggtattc ttcaagatga agccgatcca atggtctctg ttatgaaagt cgataaggct ccacttgaat catattcaca aatcggaggt ttggaagagc aaattcaaga aattaaggaa gctgtcgagt tcccattgac tcaccctgaa ctttatgagg atattggtat taaaccacca aagggagtga ttctttatgg agaaccaggt acaggaaaga cattacttgc aaaagctgtt gctcaccaca ctagtgctac attcttgcgt gtggttggat cagagttgat tcaaaagtat cttggagagg gtcctaaact cgttcgtgaa ctgttcagag ttgcagaaga attagcacca agtattgtat ttattgacga aattgatgct atcggtacaa agagatttga ctctacttct ggaggtgaac gtgagattca aagaactatg cttgaattat tgaatcaatt ggatggtttt gattcaagag gagatgtcaa agtaattatg gctacaaata gaattgagtc tttggataca gccttgatca gaccaggtag aattgaccgt aagataaagt tcccattccc tgacattaag acaaagagaa agatctttga aattcataca cgtaagatga acaaggctgc agatgtaaat ttggatgaat ttatctcatc aaaggatgaa ctttctggtg ctgacattaa ggctgtgtgc acagaagcag gtttacttgc tctcagagaa cgtagacttc aagtgacaca agacgacttc accaaagcca aagaaaaggt gctgtataaa aagcaacaag gcattccaga aggtctctac atttgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat