NafoA.01088.e
heterotrimeric G-protein alpha subunit Galpha7
| CENTER ID: | NafoA.01088.e |
| ORGANISM: | Naegleria fowleri ATCC 30863 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| NafoA.01088.e.B1.GE41661 | full length | 1 | 321 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Structures
6NE6
DEPOSITED: 12/17/2018
DETERMINATION: XRay
CLONE: NafoA.01088.e.B1.GE41661
PROTEIN: NafoA.01088.e.B1.PW38434
Publications by SSGCID
Naegleria fowleri: Protein structures to facilitate drug discovery for the deadly, pathogenic free-living amoeba
Abendroth J, Barrett LK, Dranow DM, Edwards TE, Myler PJ, Phan IQ, Staker B, Subramanian S, Tran N, Van Voorhis WC, Craig JC, Smith C, Chun IKH, Kyle D, Barrett KF, Morris JC, Tillery L, Rice CA, Goldstein J, Lassner JW, Xu L, Young RM, Osterhout B, Leahy JW, Manetsch R
PLoS ONE - 2021
volume 16, issue 3, pages e0241738
PMID: 33760815; PMCID: PMC7990177
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| EuPathDB: | AmoebaDB:NF0037180 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKKILLVGAG DSGKSTIFKQ MRLIYNNGFT TEEKTKIKPM IYENILRNMM VLIQATKSLN LPISTEENRQ RAEKIEGLEQ KILLTTDKVW DSNLAQDIAE LWKEPAILQA MARRNEFQIE DSASYYFDNL DRIGKDDYMP SEEDVVRARI KTTGLIEQPF TFKNLPFCMI DVGGQRTERK KWIHHFQGVD SILFVCSLSE FDQKCYEDDS TNRMKESLSL FGDYINSKWF ADTLVYLLFN KHDLFVSKIK NGSRLADTFE EYKGPENDPD AAKEFIKDMY KEKDTENRLR DVFFVTALDK ANLKERLEQI TSDILSAEND L
NT Sequence
atgaagaaga ttttgttggt aggagcaggt gatagcggaa agagtaccat ctttaaacag atgagactca tttacaacaa tggcttcacg acagaagaga agaccaagat taaaccaatg atctatgaaa acattttgcg taatatgatg gtcctcattc aagcaaccaa atctctcaac cttcctattt caactgaaga gaacagacaa cgagcagaaa agattgaagg attggaacaa aagattttgt tgacaacaga caaagtctgg gattccaact tggctcaaga cattgccgag ttgtggaagg aaccagcaat tctccaagca atggcaagaa gaaatgaatt ccaaattgaa gattctgcct catactactt tgataattta gatagaatcg gaaaggatga ctatatgcca agcgaagaag atgtagttag agcaagaatc aagactacag gtttgattga acaacctttc acattcaaga atcttccatt ctgtatgatt gacgtgggag gacaaagaac agaaagaaaa aagtggattc accattttca aggtgttgac agcattctct ttgtttgctc attgagtgag ttcgatcaaa agtgttacga agacgacagc acaaacagaa tgaaggaaag tttgtctctc tttggagact acatcaactc caagtggttt gctgatacac tagtttactt actttttaac aagcatgatc tgtttgtgtc caagattaag aacggttctc gtttggctga tacatttgag gaatataagg gacctgaaaa cgatcctgat gctgctaaag aatttatcaa agatatgtac aaagaaaagg acactgaaaa cagattgcga gatgtgttct ttgtgactgc attagacaag gcaaatttga aagagcgttt agaacagatt acatctgaca tcctttcagc agagaatgat ctttaa
Details for NafoA.01088.e.B1.GE41661
| HARVESTED ON: | 6/23/2017 |
| SEQUENCED ON: | 6/28/2017 |
| EXPECTED MW: | 38kDa |
| OBSERVED MW: | 38kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 98 |
| PERCENT COVERAGE: | 96 |
Validated AA Sequence
MAHHHHHHXX XILLVGAGDS GKSTIFKQMR LIYNNGFTTE EKTKIKPMIY ENILRNMMVL IQATKSLNLP ISTEENRQRA EKIEGLEQKI LLTTDKVWDS NLAQDIAELW KEPAILQAMA RRNEFQIEDS ASYYFDNLDR IGKDDYMPSE EDVVRARIKT TGLIEQPFTF KNLPFCMIDV GGQRTERKKW IHHFQGVDSI LFVCSLSEFD QKCYEDDSTN RMKESLSLFG DYINSKWFAD TLVYLLLNKH DLFVSKIKNG SRLADTFEEY KGPENDPDAA KEFIKDMYKE KDTENRLRDV FFVTALDKAN LKERLEQ
Validated NT Sequence
tctgttctaa acgctctttc aaatttgcct tgtctaatgc agtcacaaag aacacatctc gcaatctgtt ttcagtgtcc ttttctttgt acatatcttt gataaattct ttagcagcat caggatcgtt ttcaggtccc ttatattcct caaatgtatc agccaaacga gaaccgttct taatcttgga cacaaacaga tcatgcttgt taagaagtaa gtaaactagt gtatcagcaa accacttgga gttgatgtag tctccaaaga gagacaaact ttccttcatt ctgtttgtgc tgtcgtcttc gtaacacttt tgatcgaact cactcaatga gcaaacaaag agaatgctgt caacaccttg aaaatggtga atccactttt ttctttctgt tctttgtcct cccacgtcaa tcatacagaa tggaagattc ttgaatgtga aaggttgttc aatcaaacct gtagtcttga ttcttgctct aactacatct tcttcgcttg gcatatagtc atcctttccg attctatcta aattatcaaa gtagtatgag gcagaatctt caatttggaa ttcatttctt cttgccattg cttggagaat tgctggttcc ttccacaact cggcaatgtc ttgagccaag ttggaatccc agactttgtc tgttgtcaac aaaatctttt gttccaatcc ttcaatcttt tctgctcgtt gtctgttctc ttcagttgaa ataggaaggt tgagagattt ggttgcttga atgaggacca tcatattacg caaaatgttt tcatagatca ttggtttaat cttggtcttc tcttctgtcg tgaagccatt gttgtaaatg agtctcatct gtttaaagat ggtactcttt ccgctatcac ctgctcctac caacaaaatc nnnnnnatat ggtggtggtg gtggtgagcc atggtatat
Expected Protein Sequence
MAHHHHHHMK KILLVGAGDS GKSTIFKQMR LIYNNGFTTE EKTKIKPMIY ENILRNMMVL IQATKSLNLP ISTEENRQRA EKIEGLEQKI LLTTDKVWDS NLAQDIAELW KEPAILQAMA RRNEFQIEDS ASYYFDNLDR IGKDDYMPSE EDVVRARIKT TGLIEQPFTF KNLPFCMIDV GGQRTERKKW IHHFQGVDSI LFVCSLSEFD QKCYEDDSTN RMKESLSLFG DYINSKWFAD TLVYLLFNKH DLFVSKIKNG SRLADTFEEY KGPENDPDAA KEFIKDMYKE KDTENRLRDV FFVTALDKAN LKERLEQITS DILSAENDL
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaga agattttgtt ggtaggagca ggtgatagcg gaaagagtac catctttaaa cagatgagac tcatttacaa caatggcttc acgacagaag agaagaccaa gattaaacca atgatctatg aaaacatttt gcgtaatatg atggtcctca ttcaagcaac caaatctctc aaccttccta tttcaactga agagaacaga caacgagcag aaaagattga aggattggaa caaaagattt tgttgacaac agacaaagtc tgggattcca acttggctca agacattgcc gagttgtgga aggaaccagc aattctccaa gcaatggcaa gaagaaatga attccaaatt gaagattctg cctcatacta ctttgataat ttagatagaa tcggaaagga tgactatatg ccaagcgaag aagatgtagt tagagcaaga atcaagacta caggtttgat tgaacaacct ttcacattca agaatcttcc attctgtatg attgacgtgg gaggacaaag aacagaaaga aaaaagtgga ttcaccattt tcaaggtgtt gacagcattc tctttgtttg ctcattgagt gagttcgatc aaaagtgtta cgaagacgac agcacaaaca gaatgaagga aagtttgtct ctctttggag actacatcaa ctccaagtgg tttgctgata cactagttta cttacttttt aacaagcatg atctgtttgt gtccaagatt aagaacggtt ctcgtttggc tgatacattt gaggaatata agggacctga aaacgatcct gatgctgcta aagaatttat caaagatatg tacaaagaaa aggacactga aaacagattg cgagatgtgt tctttgtgac tgcattagac aaggcaaatt tgaaagagcg tttagaacag attacatctg acatcctttc agcagagaat gatctttgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t