NafoA.01088.c

heterotrimeric G-protein alpha subunit Galpha5

CENTER ID: NafoA.01088.c
ORGANISM: Naegleria fowleri ATCC 30863
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIB

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
NafoA.01088.c.B2.GE41658 Tier 0 construct 60 394
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
NafoA.01088.c.B2.PW38410 Tier 0 construct 60 394

External Resources

RESOURCE REFERENCE ID
EuPathDB: AmoebaDB:NF0047520

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MGACFGKEGS SSSNNNNSKS QNNNSHTARV HNNNSSHLDN DTAADMDMEE NANPNSGNYD VKLLLLGSGE SGKSTFFKQV RIISQRPFSE IERRRVKNTV HFNVVSSMAV MCQKLKDLNE HDQLSEELQQ CSERVLALSK NRSFMTSTTA ASKGGERNFY TEEIANDIEK LWNNELIQRV FKTHRNQVHV FDGAEYFFER LDRIKRSDYV PSEEDLLHCR QKTIGIVEVS FEFEGLKFSL FDVGGQRSER KKWVNCFEGV SAVVYVSSLA DYDLKMYEDD SLNRMDDSLN VFEGLCNGYF KETPIIIVLN KTDIFKKKLQ VHGIDTCFSD YKGDLSNYEE SVQFIRQKFL ALNKFDEQRI WMHECCATNT EDVRVVFDKI KHQVADWKKK HSNK
NT Sequence
atgggcgcct gttttggaaa agaaggctct tcctcctcta acaataataa ctctaaatct caaaacaaca actctcacac cgcacgtgtt cacaacaaca actcttctca tctcgacaat gacactgctg ctgacatgga catggaagaa aatgccaatc ccaattctgg taattacgat gtcaaattat tgttattagg aagtggtgaa tctggtaaaa gtaccttctt caaacaagtg agaattattt cacaaagacc attctctgaa attgaaagaa gacgtgtgaa aaacactgtt catttcaatg ttgtttcatc catggctgtc atgtgtcaaa aattaaaaga tttaaatgaa catgaccaat taagtgaaga attacaacaa tgttcagaac gtgtattagc attgagtaag aatcgatcat tcatgacatc cacaacagct gccagtaagg gtggtgaacg taatttctat accgaagaaa ttgccaatga tattgaaaag ttatggaaca atgaactcat acaacgagtc tttaaaactc atcgaaatca agttcatgtt tttgatggag cagagtattt ctttgagaga ttggatcgaa tcaaacgttc tgattatgtt ccaagtgaag aagatttact tcattgtcgt caaaagacca ttggaattgt ggaagtgtct tttgaatttg aaggattgaa attttcctta tttgatgtgg gaggtcaacg atccgaacgt aagaaatggg tcaattgttt tgaaggagtg agtgcagtgg tctatgtcag ttcattggct gattatgatt tgaaaatgta tgaagatgat tctctgaatc gaatggatga ctctctcaac gtctttgaag gtctctgtaa tggatatttc aaagagactc caattattat tgtcttgaat aagacagata ttttcaaaaa aaagttacaa gtgcatggca ttgacacttg cttcagtgat tataagggtg atctttccaa ttatgaagaa tcggttcaat tcattcgtca gaagtttttg gctctcaaca aatttgatga acagagaatt tggatgcatg aatgttgtgc gacaaataca gaagatgtac gtgtggtgtt tgataagatt aaacatcaag tggctgattg gaaaaagaaa cattccaata aataa
Details for NafoA.01088.c.B2.GE41658
HARVESTED ON: 6/23/2017
SEQUENCED ON: 6/28/2017
EXPECTED MW: 40kDa
OBSERVED MW: 40kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Moderate Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 96
Validated AA Sequence
MAHHHHHHMD VKLLLLGSGE SGKSTFFKQV RIISQRPFSE IERRRVKNTV HFNVVSSMAV MCQKLKDLNE HDQLSEELQQ CSERVLALSK NRSFMTSTTA ASKGGERNFY TEEIANDIEK LWNNELIQRV FKTHRNQVHV FDGAEYFFER LDRIKRSDYV PSEEDLLHCR QKTIGIVEVS FEFEGLKFSL FDVGGQRSER KKWVNCFEGV SAVVYVSSLA DYDLKMYEDD SLNRMDDSLN VFEGLCNGYF KETPIIIVLN KTDIFKKKLQ VHGIDTCFSD YKGDLSNYEE SVQFIRQKFL ALNKFDEQRI WMHECCATNT EDVRVVFDKI KH
Validated NT Sequence
gatgtttaat cttatcaaac accacacgta catcttctgt atttgtcgca caacattcat gcatccaaat tctctgttca tcaaatttgt tgagagccaa aaacttctga cgaatgaatt gaaccgattc ttcataattg gaaagatcac ccttataatc actgaaacaa gtgtcaatgc catgcacttg taactttttt ttgaaaatat ctgtcttatt caagacaata ataattggag tctctttgaa atatccatta cagagacctt caaagacgtt gagagagtca tccattcgat tcagagaatc atcttcatac attttcaaat cataatcagc caatgaactg acatagacca ctgcactcac tccttcaaaa caattgaccc atttcttacg ttcggatcgt tgacctccca catcaaataa ggaaaatttc aatccttcaa attcaaaaga cacttccaca attccaatgg tcttttgacg acaatgaagt aaatcttctt cacttggaac ataatcagaa cgtttgattc gatccaatct ctcaaagaaa tactctgctc catcaaaaac atgaacttga tttcgatgag ttttaaagac tcgttgtatg agttcattgt tccataactt ttcaatatca ttggcaattt cttcggtata gaaattacgt tcaccaccct tactggcagc tgttgtggat gtcatgaatg atcgattctt actcaatgct aatacacgtt ctgaacattg ttgtaattct tcacttaatt ggtcatgttc atttaaatct tttaattttt gacacatgac agccatggat gaaacaacat tgaaatgaac agtgtttttc acacgtcttc tttcaatttc agagaatggt ctttgtgaaa taattctcac ttgtttgaag aaggtacttt taccagattc accacttcct aataacaata atttgacatc catatggtgg tggtggtggt gagccat
Expected Protein Sequence
MAHHHHHHDV KLLLLGSGES GKSTFFKQVR IISQRPFSEI ERRRVKNTVH FNVVSSMAVM CQKLKDLNEH DQLSEELQQC SERVLALSKN RSFMTSTTAA SKGGERNFYT EEIANDIEKL WNNELIQRVF KTHRNQVHVF DGAEYFFERL DRIKRSDYVP SEEDLLHCRQ KTIGIVEVSF EFEGLKFSLF DVGGQRSERK KWVNCFEGVS AVVYVSSLAD YDLKMYEDDS LNRMDDSLNV FEGLCNGYFK ETPIIIVLNK TDIFKKKLQV HGIDTCFSDY KGDLSNYEES VQFIRQKFLA LNKFDEQRIW MHECCATNTE DVRVVFDKIK HQVADWKKKH SNK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgatgtca aattattgtt attaggaagt ggtgaatctg gtaaaagtac cttcttcaaa caagtgagaa ttatttcaca aagaccattc tctgaaattg aaagaagacg tgtgaaaaac actgttcatt tcaatgttgt ttcatccatg gctgtcatgt gtcaaaaatt aaaagattta aatgaacatg accaattaag tgaagaatta caacaatgtt cagaacgtgt attagcattg agtaagaatc gatcattcat gacatccaca acagctgcca gtaagggtgg tgaacgtaat ttctataccg aagaaattgc caatgatatt gaaaagttat ggaacaatga actcatacaa cgagtcttta aaactcatcg aaatcaagtt catgtttttg atggagcaga gtatttcttt gagagattgg atcgaatcaa acgttctgat tatgttccaa gtgaagaaga tttacttcat tgtcgtcaaa agaccattgg aattgtggaa gtgtcttttg aatttgaagg attgaaattt tccttatttg atgtgggagg tcaacgatcc gaacgtaaga aatgggtcaa ttgttttgaa ggagtgagtg cagtggtcta tgtcagttca ttggctgatt atgatttgaa aatgtatgaa gatgattctc tgaatcgaat ggatgactct ctcaacgtct ttgaaggtct ctgtaatgga tatttcaaag agactccaat tattattgtc ttgaataaga cagatatttt caaaaaaaag ttacaagtgc atggcattga cacttgcttc agtgattata agggtgatct ttccaattat gaagaatcgg ttcaattcat tcgtcagaag tttttggctc tcaacaaatt tgatgaacag agaatttgga tgcatgaatg ttgtgcgaca aatacagaag atgtacgtgt ggtgtttgat aagattaaac atcaagtggc tgattggaaa aagaaacatt ccaataaatg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat
Details for NafoA.01088.c.B2.PW38410
PURIFICATION DATe: 1/8/2018
CONCENTRATION: 16.95mg/ml
OBSERVED MW: 39kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 25 mM HEPES pH 7.5, 500 mM NaCl, 5% Glycerol , 2 mM DTT, 0.025% Azide, 5uM GDP, 10mM NaF, 30uM AlCl3, 10mM MgCl3
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 8
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 96
Protocol Notes
Repeat purification, Dual pellet purification 43379 and 43380
Validated AA Sequence
MAHHHHHHMD VKLLLLGSGE SGKSTFFKQV RIISQRPFSE IERRRVKNTV HFNVVSSMAV MCQKLKDLNE HDQLSEELQQ CSERVLALSK NRSFMTSTTA ASKGGERNFY TEEIANDIEK LWNNELIQRV FKTHRNQVHV FDGAEYFFER LDRIKRSDYV PSEEDLLHCR QKTIGIVEVS FEFEGLKFSL FDVGGQRSER KKWVNCFEGV SAVVYVSSLA DYDLKMYEDD SLNRMDDSLN VFEGLCNGYF KETPIIIVLN KTDIFKKKLQ VHGIDTCFSD YKGDLSNYEE SVQFIRQKFL ALNKFDEQRI WMHECCATNT EDVRVVFDKI KH
Validated NT Sequence
gatgtttaat cttatcaaac accacacgta catcttctgt atttgtcgca caacattcat gcatccaaat tctctgttca tcaaatttgt tgagagccaa aaacttctga cgaatgaatt gaaccgattc ttcataattg gaaagatcac ccttataatc actgaaacaa gtgtcaatgc catgcacttg taactttttt ttgaaaatat ctgtcttatt caagacaata ataattggag tctctttgaa atatccatta cagagacctt caaagacgtt gagagagtca tccattcgat tcagagaatc atcttcatac attttcaaat cataatcagc caatgaactg acatagacca ctgcactcac tccttcaaaa caattgaccc atttcttacg ttcggatcgt tgacctccca catcaaataa ggaaaatttc aatccttcaa attcaaaaga cacttccaca attccaatgg tcttttgacg acaatgaagt aaatcttctt cacttggaac ataatcagaa cgtttgattc gatccaatct ctcaaagaaa tactctgctc catcaaaaac atgaacttga tttcgatgag ttttaaagac tcgttgtatg agttcattgt tccataactt ttcaatatca ttggcaattt cttcggtata gaaattacgt tcaccaccct tactggcagc tgttgtggat gtcatgaatg atcgattctt actcaatgct aatacacgtt ctgaacattg ttgtaattct tcacttaatt ggtcatgttc atttaaatct tttaattttt gacacatgac agccatggat gaaacaacat tgaaatgaac agtgtttttc acacgtcttc tttcaatttc agagaatggt ctttgtgaaa taattctcac ttgtttgaag aaggtacttt taccagattc accacttcct aataacaata atttgacatc catatggtgg tggtggtggt gagccat
Expressed Protein Sequence
MAHHHHHHDV KLLLLGSGES GKSTFFKQVR IISQRPFSEI ERRRVKNTVH FNVVSSMAVM CQKLKDLNEH DQLSEELQQC SERVLALSKN RSFMTSTTAA SKGGERNFYT EEIANDIEKL WNNELIQRVF KTHRNQVHVF DGAEYFFERL DRIKRSDYVP SEEDLLHCRQ KTIGIVEVSF EFEGLKFSLF DVGGQRSERK KWVNCFEGVS AVVYVSSLAD YDLKMYEDDS LNRMDDSLNV FEGLCNGYFK ETPIIIVLNK TDIFKKKLQV HGIDTCFSDY KGDLSNYEES VQFIRQKFLA LNKFDEQRIW MHECCATNTE DVRVVFDKIK HQVADWKKKH SNK
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATGATGTCA AATTATTGTT ATTAGGAAGT GGTGAATCTG GTAAAAGTAC CTTCTTCAAA CAAGTGAGAA TTATTTCACA AAGACCATTC TCTGAAATTG AAAGAAGACG TGTGAAAAAC ACTGTTCATT TCAATGTTGT TTCATCCATG GCTGTCATGT GTCAAAAATT AAAAGATTTA AATGAACATG ACCAATTAAG TGAAGAATTA CAACAATGTT CAGAACGTGT ATTAGCATTG AGTAAGAATC GATCATTCAT GACATCCACA ACAGCTGCCA GTAAGGGTGG TGAACGTAAT TTCTATACCG AAGAAATTGC CAATGATATT GAAAAGTTAT GGAACAATGA ACTCATACAA CGAGTCTTTA AAACTCATCG AAATCAAGTT CATGTTTTTG ATGGAGCAGA GTATTTCTTT GAGAGATTGG ATCGAATCAA ACGTTCTGAT TATGTTCCAA GTGAAGAAGA TTTACTTCAT TGTCGTCAAA AGACCATTGG AATTGTGGAA GTGTCTTTTG AATTTGAAGG ATTGAAATTT TCCTTATTTG ATGTGGGAGG TCAACGATCC GAACGTAAGA AATGGGTCAA TTGTTTTGAA GGAGTGAGTG CAGTGGTCTA TGTCAGTTCA TTGGCTGATT ATGATTTGAA AATGTATGAA GATGATTCTC TGAATCGAAT GGATGACTCT CTCAACGTCT TTGAAGGTCT CTGTAATGGA TATTTCAAAG AGACTCCAAT TATTATTGTC TTGAATAAGA CAGATATTTT CAAAAAAAAG TTACAAGTGC ATGGCATTGA CACTTGCTTC AGTGATTATA AGGGTGATCT TTCCAATTAT GAAGAATCGG TTCAATTCAT TCGTCAGAAG TTTTTGGCTC TCAACAAATT TGATGAACAG AGAATTTGGA TGCATGAATG TTGTGCGACA AATACAGAAG ATGTACGTGT GGTGTTTGAT AAGATTAAAC ATCAAGTGGC TGATTGGAAA AAGAAACATT CCAATAAATG AGTAAGATAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAATTCTTG AAGACGAAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTGTTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGCAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAT ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGT ATACACTCCG CTATCGCTAC GTGACTGGGT CATGGCTGCG CCCCGACACC CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC GCGCGAGGCA GCTGCGGTAA AGCTCATCAG CGTGGTCGTG AAGCGATTCA CAGATGTCTG CCTGTTCATC CGCGTCCAGC TCGTTGAGTT TCTCCAGAAG CGTTAATGTC TGGCTTCTGA TAAAGCGGGC CATGTTAAGG GCGGTTTTTT CCTGTTTGGT CACTGATGCC TCCGTGTAAG GGGGATTTCT GTTCATGGGG GTAATGATAC CGATGAAACG AGAGAGGATG CTCACGATAC GGGTTACTGA TGATGAACAT GCCCGGTTAC TGGAACGTTG TGAGGGTAAA CAACTGGCGG TATGGATGCG GCGGGACCAG AGAAAAATCA CTCAGGGTCA ATGCCAGCGC TTCGTTAATA CAGATGTAGG TGTTCCACAG GGTAGCCAGC AGCATCCTGC GATGCAGATC CGGAACATAA TGGTGCAGGG CGCTGACTTC CGCGTTTCCA GACTTTACGA AACACGGAAA CCGAAGACCA TTCATGTTGT TGCTCAGGTC GCAGACGTTT TGCAGCAGCA GTCGCTTCAC GTTCGCTCGC GTATCGGTGA TTCATTCTGC TAACCAGTAA GGCAACCCCG CCAGCCTAGC CGGGTCCTCA ACGACAGGAG CACGATCATG CGCACCCGTG GCCAGGACCC AACGCTGCCC GAGATGCGCC GCGTGCGGCT GCTGGAGATG GCGGACGCGA TGGATATGTT CTGCCAAGGG TTGGTTTGCG CATTCACAGT TCTCCGCAAG AATTGATTGG CTCCAATTCT TGGAGTGGTG AATCCGTTAG CGAGGTGCCG CCGGCTTCCA TTCAGGTCGA GGTGGCCCGG CTCCATGCAC CGCGACGCAA CGCGGGGAGG CAGACAAGGT ATAGGGCGGC GCCTACAATC CATGCCAACC CGTTCCATGT GCTCGCCGAG GCGGCATAAA TCGCCGTGAC GATCAGCGGT CCAGTGATCG AAGTTAGGCT GGTAAGAGCC GCGAGCGATC CTTGAAGCTG TCCCTGATGG TCGTCATCTA CCTGCCTGGA CAGCATGGCC TGCAACGCGG GCATCCCGAT GCCGCCGGAA GCGAGAAGAA TCATAATGGG GAAGGCCATC CAGCCTCGCG TCGCGAACGC CAGCAAGACG TAGCCCAGCG CGTCGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AAT