SSGCID: Target Page

NafoA.01088.b

heterotrimeric G-protein alpha subunit Galpha3

CENTER ID:	NafoA.01088.b
ORGANISM:	Naegleria fowleri ATCC 30863
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
NafoA.01088.b.B1.GE41656	full length		1	355
NafoA.01088.b.B2.GE41657	Tier 0 construct		30	355

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
NafoA.01088.b.B1.PW38360	full length		1	355
NafoA.01088.b.B2.PW38361	Tier 0 construct		30	355

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	AmoebaDB:NF0055440

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MGQACLGGDA GKQMDQRVTD LLESDRLKDS EIKLLLLGAG ESGKSTLFKQ IRIIHDGGYN DHELLGFVDT IHSNVLQSMQ ALIQASHDLN IPIENPENRQ KAAKILELQN DSLLNINKVY NEAFANDLKD LWEDAGIQGV YKRRNEFQLL DSTEYFYTHL KRISQKDYIP NQQDVLHCRI KTTGVVQLRF EIDGLKMLLI DVGGQRNERK KWIHCFDDVT AIIFVASLSE FDQKCYEDDS TNRLDESILL FDEIVNSHFF EKTPVILFLN KSDLFAKKLQ ERVFKEVFPD FEGDDTNLRQ CADYIKEKFL QRIEDKDEKK IDVRITCATD TEAIKTVFDN VKEAIVKNTV IGRSD

NT Sequence

atgggacaag cttgtctagg aggagatgct ggtaaacaaa tggaccagag agttactgat ttactcgaga gcgaccgatt aaaggatagc gaaattaaat tgttgttact gggagctgga gaatcaggaa aatcaactct cttcaagcaa atcagaatca tacacgacgg aggttacaat gaccacgaat tactcggttt cgtggacacc attcactcta acgttctcca atccatgcaa gcactcattc aggcctctca cgatctcaac attcccattg agaatccaga gaatagacaa aaggcagcca aaattcttga attacaaaat gattctctct tgaatatcaa caaggtgtat aatgaggcct ttgcaaatga tttgaaggat ttgtgggaag atgctggaat tcaaggagtt tacaagcgta gaaatgaatt tcaattgttg gattctactg aatactttta tacacacttg aagagaattt ctcagaaaga ttacattcca aaccaacaag acgttctcca ctgtagaata aaaactacag gtgtggtaca actgaggttc gagattgatg gactcaaaat gcttctgatc gacgtcggag gacaacgtaa cgaaagaaag aaatggattc actgtttcga tgatgtcaca gctattattt tcgttgccag tttgagtgaa tttgatcaaa agtgttatga agacgatagc actaatcgtt tggatgaatc catcctcttg tttgatgaaa ttgtaaactc tcacttcttt gagaagactc ctgttatttt gtttttgaac aagagtgact tgtttgctaa gaagttacaa gaacgtgtat tcaaggaagt tttccctgat tttgaaggag atgatactaa tttgagacaa tgtgctgact acataaaaga aaaattcctc caacgtatag aagataagga tgaaaagaag attgatgtca ggataacatg cgcaactgat acagaagcaa tcaagactgt atttgacaat gtcaaggaag ctattgtcaa gaacactgtt attggtcgat ctgattaa

Details for NafoA.01088.b.B1.GE41656

HARVESTED ON:	6/23/2017
SEQUENCED ON:	6/28/2017
EXPECTED MW:	42kDa
OBSERVED MW:	42kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	72

Validated AA Sequence

MQALIQASHD LNIPIENPEN RQKAAKILEL QNDSLLNINK VYNEAFANDL KDLWEDAGIQ GVYKRRNEFQ LLDSTEYFYT HLKRISQKDY IPNQQDVLHC RIKTTGVVQL RFEIDGLKML LIDVGGQRNE RKKWIHCFDD VTAIIFVASL SEFDQKCYED DSTNRLDESI LLFDEIVNSH FFEKTPVILF LNKSDLFAKK LQERVFKEVF PDFEGDDTNL RQCADYIKEK FLQRIEDKDE KKIDVRITCA TDTEAIKTVF DNV

Validated NT Sequence

tgacattgtc aaatacagtc ttgattgctt ctgtatcagt tgcgcatgtt atcctgacat caatcttctt ttcatcctta tcttctatac gttggaggaa tttttctttt atgtagtcag cacattgtct caaattagta tcatctcctt caaaatcagg gaaaacttcc ttgaatacac gttcttgtaa cttcttagca aacaagtcac tcttgttcaa aaacaaaata acaggagtct tctcaaagaa gtgagagttt acaatttcat caaacaagag gatggattca tccaaacgat tagtgctatc gtcttcataa cacttttgat caaattcact caaactggca acgaaaataa tagctgtgac atcatcgaaa cagtgaatcc atttctttct ttcgttacgt tgtcctccga cgtcgatcag aagcattttg agtccatcaa tctcgaacct cagttgtacc acacctgtag tttttattct acagtggaga acgtcttgtt ggtttggaat gtaatctttc tgagaaattc tcttcaagtg tgtataaaag tattcagtag aatccaacaa ttgaaattca tttctacgct tgtaaactcc ttgaattcca gcatcttccc acaaatcctt caaatcattt gcaaaggcct cattatacac cttgttgata ttcaagagag aatcattttg taattcaaga attttggctg ccttttgtct attctctgga ttctcaatgg gaatgttgag atcgtgagag gcctgaatga gtgcttgcat ggattggaga acgttagagt gaatggtgtc cacgaaaccg agtaattcnn ngtcattgta acctccgtcg tgtatgattc tgatnngntt gaagagagtt nnantttcct gattctccag ctgccagtaa caacaattta atttcgctat cctttaatcg gtcgctcnng agtaaatcag taactctctg gtccnnnnnt ttaccagcat ctnnnnctan nnaagcttgt cccatatggn nnnnnnggtg gtgagc

Expected Protein Sequence

MAHHHHHHMG QACLGGDAGK QMDQRVTDLL ESDRLKDSEI KLLLLGAGES GKSTLFKQIR IIHDGGYNDH ELLGFVDTIH SNVLQSMQAL IQASHDLNIP IENPENRQKA AKILELQNDS LLNINKVYNE AFANDLKDLW EDAGIQGVYK RRNEFQLLDS TEYFYTHLKR ISQKDYIPNQ QDVLHCRIKT TGVVQLRFEI DGLKMLLIDV GGQRNERKKW IHCFDDVTAI IFVASLSEFD QKCYEDDSTN RLDESILLFD EIVNSHFFEK TPVILFLNKS DLFAKKLQER VFKEVFPDFE GDDTNLRQCA DYIKEKFLQR IEDKDEKKID VRITCATDTE AIKTVFDNVK EAIVKNTVIG RSD

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgggac aagcttgtct aggaggagat gctggtaaac aaatggacca gagagttact gatttactcg agagcgaccg attaaaggat agcgaaatta aattgttgtt actgggagct ggagaatcag gaaaatcaac tctcttcaag caaatcagaa tcatacacga cggaggttac aatgaccacg aattactcgg tttcgtggac accattcact ctaacgttct ccaatccatg caagcactca ttcaggcctc tcacgatctc aacattccca ttgagaatcc agagaataga caaaaggcag ccaaaattct tgaattacaa aatgattctc tcttgaatat caacaaggtg tataatgagg cctttgcaaa tgatttgaag gatttgtggg aagatgctgg aattcaagga gtttacaagc gtagaaatga atttcaattg ttggattcta ctgaatactt ttatacacac ttgaagagaa tttctcagaa agattacatt ccaaaccaac aagacgttct ccactgtaga ataaaaacta caggtgtggt acaactgagg ttcgagattg atggactcaa aatgcttctg atcgacgtcg gaggacaacg taacgaaaga aagaaatgga ttcactgttt cgatgatgtc acagctatta ttttcgttgc cagtttgagt gaatttgatc aaaagtgtta tgaagacgat agcactaatc gtttggatga atccatcctc ttgtttgatg aaattgtaaa ctctcacttc tttgagaaga ctcctgttat tttgtttttg aacaagagtg acttgtttgc taagaagtta caagaacgtg tattcaagga agttttccct gattttgaag gagatgatac taatttgaga caatgtgctg actacataaa agaaaaattc ctccaacgta tagaagataa ggatgaaaag aagattgatg tcaggataac atgcgcaact gatacagaag caatcaagac tgtatttgac aatgtcaagg aagctattgt caagaacact gttattggtc gatctgattg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat

Details for NafoA.01088.b.B2.GE41657

HARVESTED ON:	6/23/2017
SEQUENCED ON:	6/28/2017
EXPECTED MW:	39kDa
OBSERVED MW:	39kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	96

Validated AA Sequence

MAHHHHHHMS EIKLLLLGAG ESGKSTLFKQ IRIIHDGGYN DHELLGFVDT IHSNVLQSMQ ALIQASHDLN IPIENPENRQ KAAKILELQN DSLLNINKVY NEAFANDLKD LWEDAGIQGV YKRRNEFQLL DSTEYFYTHL KRISQKDYIP NQQDVLHCRI KTTGVVQLRF EIDGLKMLLI DVGGQRNERK KWIHCFDDVT AIIFVASLSE FDQKCYEDDS TNRLDESILL FDEIVNSHFF EKTPVILFLN KSDLFAKKLQ ERVFKEVFPD FEGDDTNLRQ CADYIKEKFL QRIEDKDEKK IDVRITCATD TEAIKTVFDN VK

Validated NT Sequence

tccttgacat tgtcaaatac agtcttgatt gcttctgtat cagttgcgca tgttatcctg acatcaatct tcttttcatc cttatcttct atacgttgga ggaatttttc ttttatgtag tcagcacatt gtctcaaatt agtatcatct ccttcaaaat cagggaaaac ttccttgaat acacgttctt gtaacttctt agcaaacaag tcactcttgt tcaaaaacaa aataacagga gtcttctcaa agaagtgaga gtttacaatt tcatcaaaca agaggatgga ttcatccaaa cgattagtgc tatcgtcttc ataacacttt tgatcaaatt cactcaaact ggcaacgaaa ataatagctg tgacatcatc gaaacagtga atccatttct ttctttcgtt acgttgtcct ccgacgtcga tcagaagcat tttgagtcca tcaatctcga acctcagttg taccacacct gtagttttta ttctacagtg gagaacgtct tgttggtttg gaatgtaatc tttctgagaa attctcttca agtgtgtata aaagtattca gtagaatcca acaattgaaa ttcatttcta cgcttgtaaa ctccttgaat tccagcatct tcccacaaat ccttcaaatc atttgcaaag gcctcattat acaccttgtt gatattcaag agagaatcat tttgtaattc aagaattttg gctgcctttt gtctattctc tggattctca atgggaatgt tgagatcgtg agaggcctga atgagtgctt gcatggattg gagaacgtta gagtgaatgg tgtccacgaa accgagtaat tcgtggtcat tgtaacctcc gtcgtgtatg attctgattt gcttgaagag agttgatttt cctgattctc cagctcccag taacaacaat ttaatttcgc tcatatggtg gtggtggtgg tgagccat

Expected Protein Sequence

MAHHHHHHSE IKLLLLGAGE SGKSTLFKQI RIIHDGGYND HELLGFVDTI HSNVLQSMQA LIQASHDLNI PIENPENRQK AAKILELQND SLLNINKVYN EAFANDLKDL WEDAGIQGVY KRRNEFQLLD STEYFYTHLK RISQKDYIPN QQDVLHCRIK TTGVVQLRFE IDGLKMLLID VGGQRNERKK WIHCFDDVTA IIFVASLSEF DQKCYEDDST NRLDESILLF DEIVNSHFFE KTPVILFLNK SDLFAKKLQE RVFKEVFPDF EGDDTNLRQC ADYIKEKFLQ RIEDKDEKKI DVRITCATDT EAIKTVFDNV KEAIVKNTVI GRSD

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catagcgaaa ttaaattgtt gttactggga gctggagaat caggaaaatc aactctcttc aagcaaatca gaatcataca cgacggaggt tacaatgacc acgaattact cggtttcgtg gacaccattc actctaacgt tctccaatcc atgcaagcac tcattcaggc ctctcacgat ctcaacattc ccattgagaa tccagagaat agacaaaagg cagccaaaat tcttgaatta caaaatgatt ctctcttgaa tatcaacaag gtgtataatg aggcctttgc aaatgatttg aaggatttgt gggaagatgc tggaattcaa ggagtttaca agcgtagaaa tgaatttcaa ttgttggatt ctactgaata cttttataca cacttgaaga gaatttctca gaaagattac attccaaacc aacaagacgt tctccactgt agaataaaaa ctacaggtgt ggtacaactg aggttcgaga ttgatggact caaaatgctt ctgatcgacg tcggaggaca acgtaacgaa agaaagaaat ggattcactg tttcgatgat gtcacagcta ttattttcgt tgccagtttg agtgaatttg atcaaaagtg ttatgaagac gatagcacta atcgtttgga tgaatccatc ctcttgtttg atgaaattgt aaactctcac ttctttgaga agactcctgt tattttgttt ttgaacaaga gtgacttgtt tgctaagaag ttacaagaac gtgtattcaa ggaagttttc cctgattttg aaggagatga tactaatttg agacaatgtg ctgactacat aaaagaaaaa ttcctccaac gtatagaaga taaggatgaa aagaagattg atgtcaggat aacatgcgca actgatacag aagcaatcaa gactgtattt gacaatgtca aggaagctat tgtcaagaac actgttattg gtcgatctga ttgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat

Details for NafoA.01088.b.B1.PW38360

PURIFICATION DATe:	9/26/2017
CONCENTRATION:	62.11mg/ml
OBSERVED MW:	40kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 8.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide, 50uM GDP
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	2
VIAL VOLUME:	200µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	72

Protocol Notes

Added 50uM GDP and pH 8 to all buffers

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGGAC AAGCTTGTCT AGGAGGAGAT GCTGGTAAAC AAATGGACCA GAGAGTTACT GATTTACTCG AGAGCGACCG ATTAAAGGAT AGCGAAATTA AATTGTTGTT ACTGGGAGCT GGAGAATCAG GAAAATCAAC TCTCTTCAAG CAAATCAGAA TCATACACGA CGGAGGTTAC AATGACCACG AATTACTCGG TTTCGTGGAC ACCATTCACT CTAACGTTCT CCAATCCATG CAAGCACTCA TTCAGGCCTC TCACGATCTC AACATTCCCA TTGAGAATCC AGAGAATAGA CAAAAGGCAG CCAAAATTCT TGAATTACAA AATGATTCTC TCTTGAATAT CAACAAGGTG TATAATGAGG CCTTTGCAAA TGATTTGAAG GATTTGTGGG AAGATGCTGG AATTCAAGGA GTTTACAAGC GTAGAAATGA ATTTCAATTG TTGGATTCTA CTGAATACTT TTATACACAC TTGAAGAGAA TTTCTCAGAA AGATTACATT CCAAACCAAC AAGACGTTCT CCACTGTAGA ATAAAAACTA CAGGTGTGGT ACAACTGAGG TTCGAGATTG ATGGACTCAA AATGCTTCTG ATCGACGTCG GAGGACAACG TAACGAAAGA AAGAAATGGA TTCACTGTTT CGATGATGTC ACAGCTATTA TTTTCGTTGC CAGTTTGAGT GAATTTGATC AAAAGTGTTA TGAAGACGAT AGCACTAATC GTTTGGATGA ATCCATCCTC TTGTTTGATG AAATTGTAAA CTCTCACTTC TTTGAGAAGA CTCCTGTTAT TTTGTTTTTG AACAAGAGTG ACTTGTTTGC TAAGAAGTTA CAAGAACGTG TATTCAAGGA AGTTTTCCCT GATTTTGAAG GAGATGATAC TAATTTGAGA CAATGTGCTG ACTACATAAA AGAAAAATTC CTCCAACGTA TAGAAGATAA GGATGAAAAG AAGATTGATG TCAGGATAAC ATGCGCAACT GATACAGAAG CAATCAAGAC TGTATTTGAC AATGTCAAGG AAGCTATTGT CAAGAACACT GTTATTGGTC GATCTGATTG AGTAAGATAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAATTCTTG AAGACGAAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTGTTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGCAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAT ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGT ATACACTCCG CTATCGCTAC GTGACTGGGT CATGGCTGCG CCCCGACACC CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC GCGCGAGGCA GCTGCGGTAA AGCTCATCAG CGTGGTCGTG AAGCGATTCA CAGATGTCTG CCTGTTCATC CGCGTCCAGC TCGTTGAGTT TCTCCAGAAG CGTTAATGTC TGGCTTCTGA TAAAGCGGGC CATGTTAAGG GCGGTTTTTT CCTGTTTGGT CACTGATGCC TCCGTGTAAG GGGGATTTCT GTTCATGGGG GTAATGATAC CGATGAAACG AGAGAGGATG CTCACGATAC GGGTTACTGA TGATGAACAT GCCCGGTTAC TGGAACGTTG TGAGGGTAAA CAACTGGCGG TATGGATGCG GCGGGACCAG AGAAAAATCA CTCAGGGTCA ATGCCAGCGC TTCGTTAATA CAGATGTAGG TGTTCCACAG GGTAGCCAGC AGCATCCTGC GATGCAGATC CGGAACATAA TGGTGCAGGG CGCTGACTTC CGCGTTTCCA GACTTTACGA AACACGGAAA CCGAAGACCA TTCATGTTGT TGCTCAGGTC GCAGACGTTT TGCAGCAGCA GTCGCTTCAC GTTCGCTCGC GTATCGGTGA TTCATTCTGC TAACCAGTAA GGCAACCCCG CCAGCCTAGC CGGGTCCTCA ACGACAGGAG CACGATCATG CGCACCCGTG GCCAGGACCC AACGCTGCCC GAGATGCGCC GCGTGCGGCT GCTGGAGATG GCGGACGCGA TGGATATGTT CTGCCAAGGG TTGGTTTGCG CATTCACAGT TCTCCGCAAG AATTGATTGG CTCCAATTCT TGGAGTGGTG AATCCGTTAG CGAGGTGCCG CCGGCTTCCA TTCAGGTCGA GGTGGCCCGG CTCCATGCAC CGCGACGCAA CGCGGGGAGG CAGACAAGGT ATAGGGCGGC GCCTACAATC CATGCCAACC CGTTCCATGT GCTCGCCGAG GCGGCATAAA TCGCCGTGAC GATCAGCGGT CCAGTGATCG AAGTTAGGCT GGTAAGAGCC GCGAGCGATC CTTGAAGCTG TCCCTGATGG TCGTCATCTA CCTGCCTGGA CAGCATGGCC TGCAACGCGG GCATCCCGAT GCCGCCGGAA GCGAGAAGAA TCATAATGGG GAAGGCCATC CAGCCTCGCG TCGCGAACGC CAGCAAGACG TAGCCCAGCG CGTCGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AAT

Details for NafoA.01088.b.B2.PW38361

PURIFICATION DATe:	9/26/2017
CONCENTRATION:	60.34mg/ml
OBSERVED MW:	37kDa
EXPRESSION LEVEL:	High Expression
PROTEIN PURIFICATION BUFFER:	25 mM HEPES pH 8.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide, 50uM GDP
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	4
VIAL VOLUME:	11µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	96

Protocol Notes

Added 50uM GDP and pH 8 to all buffers

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATAGCGAAA TTAAATTGTT GTTACTGGGA GCTGGAGAAT CAGGAAAATC AACTCTCTTC AAGCAAATCA GAATCATACA CGACGGAGGT TACAATGACC ACGAATTACT CGGTTTCGTG GACACCATTC ACTCTAACGT TCTCCAATCC ATGCAAGCAC TCATTCAGGC CTCTCACGAT CTCAACATTC CCATTGAGAA TCCAGAGAAT AGACAAAAGG CAGCCAAAAT TCTTGAATTA CAAAATGATT CTCTCTTGAA TATCAACAAG GTGTATAATG AGGCCTTTGC AAATGATTTG AAGGATTTGT GGGAAGATGC TGGAATTCAA GGAGTTTACA AGCGTAGAAA TGAATTTCAA TTGTTGGATT CTACTGAATA CTTTTATACA CACTTGAAGA GAATTTCTCA GAAAGATTAC ATTCCAAACC AACAAGACGT TCTCCACTGT AGAATAAAAA CTACAGGTGT GGTACAACTG AGGTTCGAGA TTGATGGACT CAAAATGCTT CTGATCGACG TCGGAGGACA ACGTAACGAA AGAAAGAAAT GGATTCACTG TTTCGATGAT GTCACAGCTA TTATTTTCGT TGCCAGTTTG AGTGAATTTG ATCAAAAGTG TTATGAAGAC GATAGCACTA ATCGTTTGGA TGAATCCATC CTCTTGTTTG ATGAAATTGT AAACTCTCAC TTCTTTGAGA AGACTCCTGT TATTTTGTTT TTGAACAAGA GTGACTTGTT TGCTAAGAAG TTACAAGAAC GTGTATTCAA GGAAGTTTTC CCTGATTTTG AAGGAGATGA TACTAATTTG AGACAATGTG CTGACTACAT AAAAGAAAAA TTCCTCCAAC GTATAGAAGA TAAGGATGAA AAGAAGATTG ATGTCAGGAT AACATGCGCA ACTGATACAG AAGCAATCAA GACTGTATTT GACAATGTCA AGGAAGCTAT TGTCAAGAAC ACTGTTATTG GTCGATCTGA TTGAGTAAGA TAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAATTC TTGAAGACGA AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTGT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGC AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGCCAGGA CCCAACGCTG CCCGAGATGC GCCGCGTGCG GCTGCTGGAG ATGGCGGACG CGATGGATAT GTTCTGCCAA GGGTTGGTTT GCGCATTCAC AGTTCTCCGC AAGAATTGAT TGGCTCCAAT TCTTGGAGTG GTGAATCCGT TAGCGAGGTG CCGCCGGCTT CCATTCAGGT CGAGGTGGCC CGGCTCCATG CACCGCGACG CAACGCGGGG AGGCAGACAA GGTATAGGGC GGCGCCTACA ATCCATGCCA ACCCGTTCCA TGTGCTCGCC GAGGCGGCAT AAATCGCCGT GACGATCAGC GGTCCAGTGA TCGAAGTTAG GCTGGTAAGA GCCGCGAGCG ATCCTTGAAG CTGTCCCTGA TGGTCGTCAT CTACCTGCCT GGACAGCATG GCCTGCAACG CGGGCATCCC GATGCCGCCG GAAGCGAGAA GAATCATAAT GGGGAAGGCC ATCCAGCCTC GCGTCGCGAA CGCCAGCAAG ACGTAGCCCA GCGCGTCGGC CGCCATGCCG GCGATAATGG CCTGCTTCTC GCCGAAACGT TTGGTGGCGG GACCAGTGAC GAAGGCTTGA GCGAGGGCGT GCAAGATTCC GAATACCGCA AGCGACAGGC CGATCATCGT CGCGCTCCAG CGAAAGCGGT CCTCGCCGAA AATGACCCAG AGCGCTGCCG GCACCTGTCC TACGAGTTGC ATGATAAAGA AGACAGTCAT AAGTGCGGCG ACGATAGTCA TGCCCCGCGC CCACCGGAAG GAGCTGACTG GGTTGAAGGC TCTCAAGGGC ATCGGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAAT