NafoA.01088.a

heterotrimeric G-protein alpha subunit

CENTER ID: NafoA.01088.a
ORGANISM: Naegleria fowleri ATCC 30863
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIB

Ordering Clones & Proteins

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Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
NafoA.01088.a.D11.PD38384 full length 1 343

External Resources

RESOURCE REFERENCE ID
EuPathDB: AmoebaDB:NF0104800

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MCFGCCESEP EIKRHIKLIL LGTAQSGKST FFKQLRVINN KPFSEKERLS MTSVVRENLM EACYSILKAM HVLNIPLASQ ENEEIASQIM TLHKESPANL SEILTTETTQ YGNMGDKICK IWKDPGMKQA LMKRYEFSIN DSADYFLDNC ERICKTDYIA TQEDIIKART KTVGITEVEF EAEGCVWKFT DVGGQREERR KWVHAFVDTT AVIFITSLND FDMMLEEDKT TNRMHESMLL FREVCDLDCF KEIPIIILFN KDDLFREKIQ KSDLRIAFSD YSGGHQYETA RDFIINKFKS LNTRNNLMYC HVTTATNTNN ISMVFNDVKN ILLQRDLQDL NII
NT Sequence
atgtgctttg gttgttgtga gagtgaaccc gaaataaaac gccatatcaa actgattcta ttgggaacgg ctcaaagcgg aaagagtacc tttttcaagc aattgagggt gatcaataac aaacccttct cggagaagga acgtctttca atgacgagtg tcgttcggga aaatcttatg gaagcttgtt actctatttt gaaggctatg catgtgttga atattcctct agcgagtcaa gaaaatgagg aaattgcaag tcaaatcatg acccttcaca aggaatctcc agcaaacctt tcagaaattc tcacaactga aaccactcag tatggaaaca tgggagacaa gatttgcaag atttggaagg atcctggcat gaagcaagct cttatgaaac gatatgaatt ttcaattaat gactcagcag attattttct ggacaattgt gagaggattt gtaaaacaga ttatattgcc actcaagaag acatcattaa agctcgaaca aaaactgttg gaattactga ggttgaattt gaggcagaag gatgtgtatg gaaattcaca gacgttggag gacagagaga agaacgaaga aaatgggtcc atgcatttgt tgacaccact gctgtcatat ttattacttc cttgaacgat tttgatatga tgttggaaga ggataagacc acgaaccgta tgcatgagag tatgttgctg ttccgagagg tgtgtgactt ggactgcttt aaggaaattc caatcatcat attattcaat aaggacgacc tattcagaga aaagattcaa aagagcgatt tgagaattgc attcagtgat tattcaggag gacatcaata tgaaacggca agggatttta tcattaacaa gttcaaatcg ctcaatacaa gaaataatct catgtactgt catgtcacta cagcaaccaa tacgaacaat atcagtatgg ttttcaatga tgtaaagaac attctccttc aacgtgactt gcaagatttg aacattattt ag
Details for NafoA.01088.a.D11.PD38384
PURIFICATION DATe: 8/12/2019
CONCENTRATION: 20mg/ml
OBSERVED MW: 51kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20mM tris pH 7.0, 200mM NaCl, 1mM TCEP
EXPRESSION HOST: BL21 DE3
VIAL COUNT (approx.): 87
VIAL VOLUME: 200µl
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MGHHHHHHSG EVKPEVKPET HINLKVSDGS SEIFFKIKKT TPLRRLMEAF AKRQGKEMDS LRFLYDGIRI QADQTPEDLD MEDNDIIEAH REQIGGSMCF GCCESEPEIK RHIKLILLGT AQSGKSTFFK QLRVINNKPF SEKERLSMTS VVRENLMEAC YSILKAMHVL NIPLASQENE EIASQIMTLH KESPANLSEI LTTETTQYGN MGDKICKIWK DPGMKQALMK RYEFSINDSA DYFLDNCERI CKTDYIATQE DIIKARTKTV GITEVEFEAE GCVWKFTDVG GQREERRKWV HAFVDTTAVI FITSLNDFDM MLEEDKTTNR MHESMLLFRE VCDLDCFKEI PIIILFNKDD LFREKIQKSD LRIAFSDYSG GHQYETARDF IINKFKSLNT RNNLMYCHVT TATNTNNISM VFNDVKNILL QRDLQDLNII
Full NT Sequence (Expression Vector + Insert)
TGGCGAATGG GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TTAACAAAAT ATTAACGTTT ACAATTTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AATTAATTCT TAGAAAAACT CATCGAGCAT CAAATGAAAC TGCAATTTAT TCATATCAGG ATTATCAATA CCATATTTTT GAAAAAGCCG TTTCTGTAAT GAAGGAGAAA ACTCACCGAG GCAGTTCCAT AGGATGGCAA GATCCTGGTA TCGGTCTGCG ATTCCGACTC GTCCAACATC AATACAACCT ATTAATTTCC CCTCGTCAAA AATAAGGTTA TCAAGTGAGA AATCACCATG AGTGACGACT GAATCCGGTG AGAATGGCAA AAGTTTATGC ATTTCTTTCC AGACTTGTTC AACAGGCCAG CCATTACGCT CGTCATCAAA ATCACTCGCA TCAACCAAAC CGTTATTCAT TCGTGATTGC GCCTGAGCGA GACGAAATAC GCGATCGCTG TTAAAAGGAC AATTACAAAC AGGAATCGAA TGCAACCGGC GCAGGAACAC TGCCAGCGCA TCAACAATAT TTTCACCTGA ATCAGGATAT TCTTCTAATA CCTGGAATGC TGTTTTCCCG GGGATCGCAG TGGTGAGTAA CCATGCATCA TCAGGAGTAC GGATAAAATG CTTGATGGTC GGAAGAGGCA TAAATTCCGT CAGCCAGTTT AGTCTGACCA TCTCATCTGT AACATCATTG GCAACGCTAC CTTTGCCATG TTTCAGAAAC AACTCTGGCG CATCGGGCTT CCCATACAAT CGATAGATTG TCGCACCTGA TTGCCCGACA TTATCGCGAG CCCATTTATA CCCATATAAA TCAGCATCCA TGTTGGAATT TAATCGCGGC CTAGAGCAAG ACGTTTCCCG TTGAATATGG CTCATAACAC CCCTTGTATT ACTGTTTATG TAAGCAGACA GTTTTATTGT TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGAGATC CCGGTGCCTA ATGAGTGAGC TAACTTACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGCATT AATGAATCGG CCAACGCGCG GGGAGAGGCG GTTTGCGTAT TGGGCGCCAG GGTGGTTTTT CTTTTCACCA GTGAGACGGG CAACAGCTGA TTGCCCTTCA CCGCCTGGCC CTGAGAGAGT TGCAGCAAGC GGTCCACGCT GGTTTGCCCC AGCAGGCGAA AATCCTGTTT GATGGTGGTT AACGGCGGGA TATAACATGA GCTGTCTTCG GTATCGTCGT ATCCCACTAC CGAGATATCC GCACCAACGC GCAGCCCGGA CTCGGTAATG GCGCGCATTG CGCCCAGCGC CATCTGATCG TTGGCAACCA GCATCGCAGT GGGAACGATG CCCTCATTCA GCATTTGCAT GGTTTGTTGA AAACCGGACA TGGCACTCCA GTCGCCTTCC CGTTCCGCTA TCGGCTGAAT TTGATTGCGA GTGAGATATT TATGCCAGCC AGCCAGACGC AGACGCGCCG AGACAGAACT TAATGGGCCC GCTAACAGCG CGATTTGCTG GTGACCCAAT GCGACCAGAT GCTCCACGCC CAGTCGCGTA CCGTCTTCAT GGGAGAAAAT AATACTGTTG ATGGGTGTCT GGTCAGAGAC ATCAAGAAAT AACGCCGGAA CATTAGTGCA GGCAGCTTCC ACAGCAATGG CATCCTGGTC ATCCAGCGGA TAGTTAATGA TCAGCCCACT GACGCGTTGC GCGAGAAGAT TGTGCACCGC CGCTTTACAG GCTTCGACGC CGCTTCGTTC TACCATCGAC ACCACCACGC TGGCACCCAG TTGATCGGCG CGAGATTTAA TCGCCGCGAC AATTTGCGAC GGCGCGTGCA GGGCCAGACT GGAGGTGGCA ACGCCAATCA GCAACGACTG TTTGCCCGCC AGTTGTTGTG CCACGCGGTT GGGAATGTAA TTCAGCTCCG CCATCGCCGC TTCCACTTTT TCCCGCGTTT TCGCAGAAAC GTGGCTGGCC TGGTTCACCA CGCGGGAAAC GGTCTGATAA GAGACACCGG CATACTCTGC GACATCGTAT AACGTTACTG GTTTCACATT CACCACCCTG AATTGACTCT CTTCCGGGCG CTATCATGCC ATACCGCGAA AGGTTTTGCG CCATTCGATG GTGTCCGGGA TCTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AATTAATACG ACTCACTATA GGGGAATTGT GAGCGGATAA CAATTCCCCT CTAGAAATAA TTTTGTTTAA CTTTAAGAAG GAGATATACC ATGGGCCACC ACCACCACCA CCATAGCGGC GAAGTAAAAC CGGAAGTGAA GCCGGAGACC CACATCAACC TGAAGGTTAG CGACGGTAGC AGCGAAATCT TCTTCAAGAT TAAGAAGACC ACCCCGCTGC GTCGCCTGAT GGAAGCATTC GCTAAACGCC AGGGCAAGGA AATGGATTCC CTGCGCTTTC TGTACGACGG TATCCGTATT CAGGCAGACC AGACTCCGGA AGATCTGGAC ATGGAAGATA ACGACATTAT CGAAGCACAC CGTGAACAAA TCGGTGGATC CATGTGCTTC GGTTGCTGCG AGAGCGAACC GGAGATTAAA CGTCACATTA AGCTGATCCT GCTGGGTACC GCGCAGAGCG GCAAAAGCAC CTTCTTTAAG CAACTGCGTG TGATCAACAA CAAACCGTTT AGCGAAAAGG AGCGTCTGAG CATGACCAGC GTGGTTCGTG AAAACCTGAT GGAGGCGTGC TACAGCATTC TGAAAGCGAT GCACGTTCTG AACATCCCGC TGGCGAGCCA GGAAAACGAG GAAATTGCGA GCCAAATCAT GACCCTGCAC AAGGAGAGCC CGGCGAACCT GAGCGAAATT CTGACCACCG AGACCACCCA GTATGGTAAC ATGGGCGACA AGATTTGCAA AATCTGGAAG GACCCGGGCA TGAAACAAGC GCTGATGAAG CGTTACGAGT TCAGCATCAA CGACAGCGCG GATTATTTTC TGGACAACTG CGAACGTATT TGCAAAACCG ACTACATCGC GACCCAGGAA GACATCATTA AAGCGCGTAC CAAGACCGTG GGTATCACCG AAGTTGAGTT CGAAGCGGAG GGCTGCGTGT GGAAATTTAC CGACGTTGGT GGCCAACGTG AGGAACGTCG TAAGTGGGTG CACGCGTTCG TTGATACCAC CGCGGTGATT TTCATCACCA GCCTGAACGA CTTTGATATG ATGCTGGAGG AAGATAAGAC CACCAACCGT ATGCACGAAA GCATGCTGCT GTTCCGTGAG GTTTGCGACC TGGATTGCTT TAAAGAAATC CCGATCATTA TCCTGTTCAA CAAGGACGAC CTGTTCCGTG AGAAAATTCA GAAGAGCGAC CTGCGTATCG CGTTCAGCGA TTACAGCGGT GGCCACCAAT ATGAGACCGC GCGTGACTTC ATTATCAACA AGTTTAAAAG CCTGAACACC CGTAACAACC TGATGTATTG CCACGTGACC ACCGCGACCA ACACCAACAA CATTAGCATG GTGTTTAACG ATGTTAAAAA CATCCTGCTG CAGCGTGACC TGCAAGATCT GAACATTATC TGATAAGCTT GCGGCCGCAC TCGAGCACCA CCACCACCAC CACTGAGATC CGGCTGCTAA CAAAGCCCGA AAGGAAGCTG AGTTGGCTGC TGCCACCGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG TTTTTTGCTG AAAGGAGGAA CTATATCCGG AT