NafoA.01037.a

ras-related protein rab-2a-like

CENTER ID: NafoA.01037.a
ORGANISM: Naegleria fowleri ATCC 30863
ASSOCIATED DISEASE:
CURRENT STATUS: purified
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
NafoA.01037.a.B11.GE40612 Full length(NafoA.01037.a) 1 215
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
NafoA.01037.a.B11.PS38435 Full length(NafoA.01037.a) 1 215

External Resources

RESOURCE REFERENCE ID
EuPathDB: AmoebaDB:NF0117540

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSYSYLFKYI IIGDTGVGKS CLLLQFTDKR FQPVHDLTIG VEFGARMINI DGKQIKLQIW DTAGQESFRS ITRSYYRGAA GALLVYDITR RETFHHLTTW LEDARRHSNS TMTIMLVGNK CDLDSRRAVS YEEGKKFAQQ HDLIFLETSA KNDENVEEAF NYTARIIYEK IEKNIIDVTN EMNGVKVGNA PTATTNLLDK PGNSSEQGRG KTCCG
NT Sequence
ATGTCCTATT CGTACTTGTT CAAATACATC ATCATTGGTG ATACAGGTGT TGGAAAGTCA TGCCTTTTAC TTCAGTTTAC CGATAAACGT TTTCAACCGG TGCACGACTT GACGATTGGA GTTGAGTTTG GAGCCAGAAT GATCAATATT GATGGAAAAC AAATCAAATT ACAAATATGG GATACTGCTG GACAAGAATC ATTCAGAAGT ATTACTCGTT CATATTACCG TGGAGCTGCT GGTGCTCTTC TCGTCTATGA CATTACGAGG CGTGAGACAT TCCATCATTT GACAACATGG CTCGAAGATG CCAGACGACA CTCCAACTCG ACAATGACCA TCATGTTGGT TGGAAACAAG TGCGATCTGG ACTCACGACG TGCTGTTTCA TATGAGGAGG GCAAAAAGTT TGCTCAACAA CATGACTTGA TCTTCTTGGA AACAAGTGCC AAGAATGATG AAAATGTCGA GGAAGCTTTC AATTACACTG CACGAATTAT TTATGAAAAA ATTGAGAAAA ATATTATTGA TGTTACCAAT GAAATGAATG GAGTGAAGGT TGGAAATGCC CCAACAGCAA CAACCAATCT TTTGGATAAG CCTGGAAACT CATCTGAACA AGGAAGGGGC AAAACTTGCT GTGGA
Details for NafoA.01037.a.B11.GE40612
HARVESTED ON: 10/18/2016
SEQUENCED ON: 10/19/2016
EXPECTED MW: 25kDa
OBSERVED MW: 25kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHHM SYSYLFKYII IGDTGVGKSC LLLQFTDKRF QPVHDLTIGV EFGARMINID GKQIKLQIWD TAGQESFRSI TRSYYRGAAG ALLVYDITRR ETFHHLTTWL EDARRHSNST MTIMLVGNKC DLDSRRAVSY EEGKKFAQQH DLIFLETSAK NDENVEEAFN YTARIIYEKI EKNIIDVTNE MNGVKVGNAP TATTNLLDKP GNSSEQGRGK TCCG
Validated NT Sequence
atcttactca cttatccaca gcaagttttg ccccttcctt gttcagatga gtttccaggc ttatccaaaa gattggttgt tgctgttggg gcatttccaa ccttcactcc attcatttca ttggtaacat caataatatt tttctcaatt ttttcataaa taattcgtgc agtgtaattg aaagcttcct cgacattttc atcattcttg gcacttgttt ccaagaagat caagtcatgt tgttgagcaa actttttgcc ctcctcatat gaaacagcac gtcgtgagtc cagatcgcac ttgtttccaa ccaacatgat ggtcattgtc gagttggagt gtcgtctggc atcttcgagc catgttgtca aatgatggaa tgtctcacgc ctcgtaatgt catagacgag aagagcacca gcagctccac ggtaatatga acgagtaata cttctgaatg attcttgtcc agcagtatcc catatttgta atttgatttg ttttccatca atattgatca ttctggctcc aaactcaact ccaatcgtca agtcgtgcac cggttgaaaa cgtttatcgg taaactgaag taaaaggcat gactttccaa cacctgtatc accaatgatg atgtatttga acaagtacga ataggacata tggtggtggt ggtggtggtg agccat
Expected Protein Sequence
MAHHHHHHMS YSYLFKYIII GDTGVGKSCL LLQFTDKRFQ PVHDLTIGVE FGARMINIDG KQIKLQIWDT AGQESFRSIT RSYYRGAAGA LLVYDITRRE TFHHLTTWLE DARRHSNSTM TIMLVGNKCD LDSRRAVSYE EGKKFAQQHD LIFLETSAKN DENVEEAFNY TARIIYEKIE KNIIDVTNEM NGVKVGNAPT ATTNLLDKPG NSSEQGRGKT CCG
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgtcct attcgtactt gttcaaatac atcatcattg gtgatacagg tgttggaaag tcatgccttt tacttcagtt taccgataaa cgttttcaac cggtgcacga cttgacgatt ggagttgagt ttggagccag aatgatcaat attgatggaa aacaaatcaa attacaaata tgggatactg ctggacaaga atcattcaga agtattactc gttcatatta ccgtggagct gctggtgctc ttctcgtcta tgacattacg aggcgtgaga cattccatca tttgacaaca tggctcgaag atgccagacg acactccaac tcgacaatga ccatcatgtt ggttggaaac aagtgcgatc tggactcacg acgtgctgtt tcatatgagg agggcaaaaa gtttgctcaa caacatgact tgatcttctt ggaaacaagt gccaagaatg atgaaaatgt cgaggaagct ttcaattaca ctgcacgaat tatttatgaa aaaattgaga aaaatattat tgatgttacc aatgaaatga atggagtgaa ggttggaaat gccccaacag caacaaccaa tcttttggat aagcctggaa actcatctga acaaggaagg ggcaaaactt gctgtggatg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat
Details for NafoA.01037.a.B11.PS38435
PURIFICATION DATe: 4/24/2018
CONCENTRATION: 19.58mg/ml
OBSERVED MW: 25kDa
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES pH 7.0, 300 mM NaCl, 5% glycerol, 1 mM TCEP
EXPRESSION HOST: BL 21 (DE3) Rosetta
VIAL COUNT (approx.): 4
VIAL VOLUME: 200µl
PERCENT IDENTITY: 99
PERCENT COVERAGE: 100
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHHM SYSYLFKYII IGDTGVGKSC LLLQFTDKRF QPVHDLTIGV EFGARMINID GKQIKLQIWD TAGQESFRSI TRSYYRGAAG ALLVYDITRR ETFHHLTTWL EDARRHSNST MTIMLVGNKC DLDSRRAVSY EEGKKFAQQH DLIFLETSAK NDENVEEAFN YTARIIYEKI EKNIIDVTNE MNGVKVGNAP TATTNLLDKP GNSSEQGRGK TCCG
Validated NT Sequence
atcttactca cttatccaca gcaagttttg ccccttcctt gttcagatga gtttccaggc ttatccaaaa gattggttgt tgctgttggg gcatttccaa ccttcactcc attcatttca ttggtaacat caataatatt tttctcaatt ttttcataaa taattcgtgc agtgtaattg aaagcttcct cgacattttc atcattcttg gcacttgttt ccaagaagat caagtcatgt tgttgagcaa actttttgcc ctcctcatat gaaacagcac gtcgtgagtc cagatcgcac ttgtttccaa ccaacatgat ggtcattgtc gagttggagt gtcgtctggc atcttcgagc catgttgtca aatgatggaa tgtctcacgc ctcgtaatgt catagacgag aagagcacca gcagctccac ggtaatatga acgagtaata cttctgaatg attcttgtcc agcagtatcc catatttgta atttgatttg ttttccatca atattgatca ttctggctcc aaactcaact ccaatcgtca agtcgtgcac cggttgaaaa cgtttatcgg taaactgaag taaaaggcat gactttccaa cacctgtatc accaatgatg atgtatttga acaagtacga ataggacata tggtggtggt ggtggtggtg agccat
Expressed Protein Sequence
MAHHHHHHMS YSYLFKYIII GDTGVGKSCL LLQFTDKRFQ PVHDLTIGVE FGARMINIDG KQIKLQIWDT AGQESFRSIT RSYYRGAAGA LLVYDITRRE TFHHLTTWLE DARRHSNSTM TIMLVGNKCD LDSRRAVSYE EGKKFAQQHD LIFLETSAKN DENVEEAFNY TARIIYEKIE KNIIDVTNEM NGVKVGNAPT ATTNLLDKPG NSSEQGRGKT CCG
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGTCCT ATTCGTACTT GTTCAAATAC ATCATCATTG GTGATACAGG TGTTGGAAAG TCATGCCTTT TACTTCAGTT TACCGATAAA CGTTTTCAAC CGGTGCACGA CTTGACGATT GGAGTTGAGT TTGGAGCCAG AATGATCAAT ATTGATGGAA AACAAATCAA ATTACAAATA TGGGATACTG CTGGACAAGA ATCATTCAGA AGTATTACTC GTTCATATTA CCGTGGAGCT GCTGGTGCTC TTCTCGTCTA TGACATTACG AGGCGTGAGA CATTCCATCA TTTGACAACA TGGCTCGAAG ATGCCAGACG ACACTCCAAC TCGACAATGA CCATCATGTT GGTTGGAAAC AAGTGCGATC TGGACTCACG ACGTGCTGTT TCATATGAGG AGGGCAAAAA GTTTGCTCAA CAACATGACT TGATCTTCTT GGAAACAAGT GCCAAGAATG ATGAAAATGT CGAGGAAGCT TTCAATTACA CTGCACGAAT TATTTATGAA AAAATTGAGA AAAATATTAT TGATGTTACC AATGAAATGA ATGGAGTGAA GGTTGGAAAT GCCCCAACAG CAACAACCAA TCTTTTGGAT AAGCCTGGAA ACTCATCTGA ACAAGGAAGG GGCAAAACTT GCTGTGGATG AGTAAGATAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAATTCTTG AAGACGAAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTGTTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGCAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAT ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGT ATACACTCCG CTATCGCTAC GTGACTGGGT CATGGCTGCG CCCCGACACC CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC GCGCGAGGCA GCTGCGGTAA AGCTCATCAG CGTGGTCGTG AAGCGATTCA CAGATGTCTG CCTGTTCATC CGCGTCCAGC TCGTTGAGTT TCTCCAGAAG CGTTAATGTC TGGCTTCTGA TAAAGCGGGC CATGTTAAGG GCGGTTTTTT CCTGTTTGGT CACTGATGCC TCCGTGTAAG GGGGATTTCT GTTCATGGGG GTAATGATAC CGATGAAACG AGAGAGGATG CTCACGATAC GGGTTACTGA TGATGAACAT GCCCGGTTAC TGGAACGTTG TGAGGGTAAA CAACTGGCGG TATGGATGCG GCGGGACCAG AGAAAAATCA CTCAGGGTCA ATGCCAGCGC TTCGTTAATA CAGATGTAGG TGTTCCACAG GGTAGCCAGC AGCATCCTGC GATGCAGATC CGGAACATAA TGGTGCAGGG CGCTGACTTC CGCGTTTCCA GACTTTACGA AACACGGAAA CCGAAGACCA TTCATGTTGT TGCTCAGGTC GCAGACGTTT TGCAGCAGCA GTCGCTTCAC GTTCGCTCGC GTATCGGTGA TTCATTCTGC TAACCAGTAA GGCAACCCCG CCAGCCTAGC CGGGTCCTCA ACGACAGGAG CACGATCATG CGCACCCGTG GCCAGGACCC AACGCTGCCC GAGATGCGCC GCGTGCGGCT GCTGGAGATG GCGGACGCGA TGGATATGTT CTGCCAAGGG TTGGTTTGCG CATTCACAGT TCTCCGCAAG AATTGATTGG CTCCAATTCT TGGAGTGGTG AATCCGTTAG CGAGGTGCCG CCGGCTTCCA TTCAGGTCGA GGTGGCCCGG CTCCATGCAC CGCGACGCAA CGCGGGGAGG CAGACAAGGT ATAGGGCGGC GCCTACAATC CATGCCAACC CGTTCCATGT GCTCGCCGAG GCGGCATAAA TCGCCGTGAC GATCAGCGGT CCAGTGATCG AAGTTAGGCT GGTAAGAGCC GCGAGCGATC CTTGAAGCTG TCCCTGATGG TCGTCATCTA CCTGCCTGGA CAGCATGGCC TGCAACGCGG GCATCCCGAT GCCGCCGGAA GCGAGAAGAA TCATAATGGG GAAGGCCATC CAGCCTCGCG TCGCGAACGC CAGCAAGACG TAGCCCAGCG CGTCGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AAT