NafoA.01013.a
phosphoglycerate mutase
| CENTER ID: | NafoA.01013.a |
| ORGANISM: | Naegleria fowleri ATCC 30863 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
Clicking the button will add the material to a virtual cart.
You may order multiple materials at a time at no cost to you, as this contract is funded
by NIAID. When you are ready to place your order, click the "Place Order" link which will
appear in the top right corner of the page after you place your first item in your cart.
Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| NafoA.01013.a.B11.GE40610 | Full length(NafoA.01013.a) | 1 | 250 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| NafoA.01013.a.B11.PS38236 | Full length(NafoA.01013.a) | 1 | 250 |
Structures
5VVE
DEPOSITED: 5/19/2017
DETERMINATION: XRay
CLONE: NafoA.01013.a.B11.GE40610
PROTEIN: NafoA.01013.a.B11.PS38236
Publications by SSGCID
Naegleria fowleri: Protein structures to facilitate drug discovery for the deadly, pathogenic free-living amoeba
Abendroth J, Barrett LK, Dranow DM, Edwards TE, Myler PJ, Phan IQ, Staker B, Subramanian S, Tran N, Van Voorhis WC, Craig JC, Smith C, Chun IKH, Kyle D, Barrett KF, Morris JC, Tillery L, Rice CA, Goldstein J, Lassner JW, Xu L, Young RM, Osterhout B, Leahy JW, Manetsch R
PLoS ONE - 2021
volume 16, issue 3, pages e0241738
PMID: 33760815; PMCID: PMC7990177
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| EuPathDB: | AmoebaDB:NF0048460 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MVYKLVLIRH GESEWNKENK FTGWYDCGLS ETGLKEAKEA GEILLKEGYE FDLCYTSFLK RAIKTLWIAL ETMDAMYLPV VKHWRLNERH YGALQGLNKT QTAEKHGEEQ VKIWRRSYDI PPPALEVTDE RYPGHERKYQ GLTQEELPKT ESLKLTVDRV LPYWNDVIAP SVKEGKRVLI AAHGNSLRAL VKYLDNISEE EIVELNIPTG VPLVYELDEN LKPIKHYYLG DQELIQQKIN SVASQASKKK
NT Sequence
ATGGTCTACA AATTGGTATT AATCAGACAC GGAGAATCCG AGTGGAACAA GGAAAACAAA TTTACTGGAT GGTACGATTG TGGATTGAGC GAAACCGGAC TCAAGGAAGC CAAAGAAGCT GGTGAAATTC TTCTCAAAGA AGGCTATGAA TTCGATTTGT GTTATACAAG CTTTTTGAAA AGAGCCATCA AAACACTATG GATTGCATTA GAAACTATGG ATGCCATGTA TCTTCCTGTT GTCAAACATT GGAGATTGAA TGAAAGACAC TATGGTGCTC TTCAAGGCTT GAACAAGACT CAAACTGCTG AGAAACACGG TGAAGAGCAA GTCAAGATTT GGAGAAGATC CTATGACATT CCTCCACCTG CTCTTGAAGT TACTGATGAG AGATATCCTG GCCATGAAAG AAAATATCAA GGATTAACTC AAGAGGAATT ACCAAAAACT GAGTCACTTA AATTGACTGT TGACAGAGTG CTCCCATATT GGAATGATGT TATTGCACCA AGCGTCAAGG AAGGCAAGAG AGTATTGATT GCTGCTCACG GTAATTCATT ACGTGCATTG GTGAAGTATT TGGATAACAT TTCTGAAGAA GAAATTGTTG AATTGAATAT TCCAACTGGT GTCCCATTGG TCTATGAATT GGATGAAAAC TTGAAGCCAA TTAAGCATTA TTACTTGGGT GATCAAGAAT TAATTCAACA AAAGATTAAC AGTGTTGCAA GTCAAGCCAG CAAGAAGAAG
Details for NafoA.01013.a.B11.GE40610
| HARVESTED ON: | 10/18/2016 |
| SEQUENCED ON: | 10/19/2016 |
| EXPECTED MW: | 30kDa |
| OBSERVED MW: | 30kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Many (50-100) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMV YKLVLIRHGE SEWNKENKFT GWYDCGLSET GLKEAKEAGE ILLKEGYEFD LCYTSFLKRA IKTLWIALET MDAMYLPVVK HWRLNERHYG ALQGLNKTQT AEKHGEEQVK IWRRSYDIPP PALEVTDERY PGHERKYQGL TQEELPKTES LKLTVDRVLP YWNDVIAPSV KEGKRVLIAA HGNSLRALVK YLDNISEEEI VELNIPTGVP LVYELDENLK PIKHYYLGDQ ELIQQKINSV ASQASKKK
Validated NT Sequence
atcttactca cttacttctt cttgctggct tgacttgcaa cactgttaat cttttgttga attaattctt gatcacccaa gtaataatgc ttaattggct tcaagttttc atccaattca tagaccaatg ggacaccagt tggaatattc aattcaacaa tttcttcttc agaaatgtta tccaaatact tcaccaatgc acgtaatgaa ttaccgtgag cagcaatcaa tactctcttg ccttccttga cgcttggtgc aataacatca ttccaatatg ggagcactct gtcaacagtc aatttaagtg actcagtttt tggtaattcc tcttgagtta atccttgata ttttctttca tggccaggat atctctcatc agtaacttca agagcaggtg gaggaatgtc ataggatctt ctccaaatct tgacttgctc ttcaccgtgt ttctcagcag tttgagtctt gttcaagcct tgaagagcac catagtgtct ttcattcaat ctccaatgtt tgacaacagg aagatacatg gcatccatag tttctaatgc aatccatagt gttttgatgg ctcttttcaa aaagcttgta taacacaaat cgaattcata gccttctttg agaagaattt caccagcttc tttggcttcc ttgagtccgg tttcgctcaa tccacaatcg taccatccag taaatttgtt ttccttgttc cactcggatt ctccgtgtct gattaatacc aatttgtaga ccatatggtg gtggtggtgg tgagccat
Expected Protein Sequence
MAHHHHHHMV YKLVLIRHGE SEWNKENKFT GWYDCGLSET GLKEAKEAGE ILLKEGYEFD LCYTSFLKRA IKTLWIALET MDAMYLPVVK HWRLNERHYG ALQGLNKTQT AEKHGEEQVK IWRRSYDIPP PALEVTDERY PGHERKYQGL TQEELPKTES LKLTVDRVLP YWNDVIAPSV KEGKRVLIAA HGNSLRALVK YLDNISEEEI VELNIPTGVP LVYELDENLK PIKHYYLGDQ ELIQQKINSV ASQASKKK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggtct acaaattggt attaatcaga cacggagaat ccgagtggaa caaggaaaac aaatttactg gatggtacga ttgtggattg agcgaaaccg gactcaagga agccaaagaa gctggtgaaa ttcttctcaa agaaggctat gaattcgatt tgtgttatac aagctttttg aaaagagcca tcaaaacact atggattgca ttagaaacta tggatgccat gtatcttcct gttgtcaaac attggagatt gaatgaaaga cactatggtg ctcttcaagg cttgaacaag actcaaactg ctgagaaaca cggtgaagag caagtcaaga tttggagaag atcctatgac attcctccac ctgctcttga agttactgat gagagatatc ctggccatga aagaaaatat caaggattaa ctcaagagga attaccaaaa actgagtcac ttaaattgac tgttgacaga gtgctcccat attggaatga tgttattgca ccaagcgtca aggaaggcaa gagagtattg attgctgctc acggtaattc attacgtgca ttggtgaagt atttggataa catttctgaa gaagaaattg ttgaattgaa tattccaact ggtgtcccat tggtctatga attggatgaa aacttgaagc caattaagca ttattacttg ggtgatcaag aattaattca acaaaagatt aacagtgttg caagtcaagc cagcaagaag aagtgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat
Details for NafoA.01013.a.B11.PS38236
| PURIFICATION DATe: | 3/23/2017 |
| CONCENTRATION: | 28.7mg/ml |
| OBSERVED MW: | 30kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | SEC Buffer: 20mM HEPES pH 7.0, 300mM NaCl,55 Glycerol, 1mM TCEP |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| VIAL COUNT (approx.): | 7 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 100 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMV YKLVLIRHGE SEWNKENKFT GWYDCGLSET GLKEAKEAGE ILLKEGYEFD LCYTSFLKRA IKTLWIALET MDAMYLPVVK HWRLNERHYG ALQGLNKTQT AEKHGEEQVK IWRRSYDIPP PALEVTDERY PGHERKYQGL TQEELPKTES LKLTVDRVLP YWNDVIAPSV KEGKRVLIAA HGNSLRALVK YLDNISEEEI VELNIPTGVP LVYELDENLK PIKHYYLGDQ ELIQQKINSV ASQASKKK
Validated NT Sequence
atcttactca cttacttctt cttgctggct tgacttgcaa cactgttaat cttttgttga attaattctt gatcacccaa gtaataatgc ttaattggct tcaagttttc atccaattca tagaccaatg ggacaccagt tggaatattc aattcaacaa tttcttcttc agaaatgtta tccaaatact tcaccaatgc acgtaatgaa ttaccgtgag cagcaatcaa tactctcttg ccttccttga cgcttggtgc aataacatca ttccaatatg ggagcactct gtcaacagtc aatttaagtg actcagtttt tggtaattcc tcttgagtta atccttgata ttttctttca tggccaggat atctctcatc agtaacttca agagcaggtg gaggaatgtc ataggatctt ctccaaatct tgacttgctc ttcaccgtgt ttctcagcag tttgagtctt gttcaagcct tgaagagcac catagtgtct ttcattcaat ctccaatgtt tgacaacagg aagatacatg gcatccatag tttctaatgc aatccatagt gttttgatgg ctcttttcaa aaagcttgta taacacaaat cgaattcata gccttctttg agaagaattt caccagcttc tttggcttcc ttgagtccgg tttcgctcaa tccacaatcg taccatccag taaatttgtt ttccttgttc cactcggatt ctccgtgtct gattaatacc aatttgtaga ccatatggtg gtggtggtgg tgagccat
Expressed Protein Sequence
MAHHHHHHMV YKLVLIRHGE SEWNKENKFT GWYDCGLSET GLKEAKEAGE ILLKEGYEFD LCYTSFLKRA IKTLWIALET MDAMYLPVVK HWRLNERHYG ALQGLNKTQT AEKHGEEQVK IWRRSYDIPP PALEVTDERY PGHERKYQGL TQEELPKTES LKLTVDRVLP YWNDVIAPSV KEGKRVLIAA HGNSLRALVK YLDNISEEEI VELNIPTGVP LVYELDENLK PIKHYYLGDQ ELIQQKINSV ASQASKKK
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGTCT ACAAATTGGT ATTAATCAGA CACGGAGAAT CCGAGTGGAA CAAGGAAAAC AAATTTACTG GATGGTACGA TTGTGGATTG AGCGAAACCG GACTCAAGGA AGCCAAAGAA GCTGGTGAAA TTCTTCTCAA AGAAGGCTAT GAATTCGATT TGTGTTATAC AAGCTTTTTG AAAAGAGCCA TCAAAACACT ATGGATTGCA TTAGAAACTA TGGATGCCAT GTATCTTCCT GTTGTCAAAC ATTGGAGATT GAATGAAAGA CACTATGGTG CTCTTCAAGG CTTGAACAAG ACTCAAACTG CTGAGAAACA CGGTGAAGAG CAAGTCAAGA TTTGGAGAAG ATCCTATGAC ATTCCTCCAC CTGCTCTTGA AGTTACTGAT GAGAGATATC CTGGCCATGA AAGAAAATAT CAAGGATTAA CTCAAGAGGA ATTACCAAAA ACTGAGTCAC TTAAATTGAC TGTTGACAGA GTGCTCCCAT ATTGGAATGA TGTTATTGCA CCAAGCGTCA AGGAAGGCAA GAGAGTATTG ATTGCTGCTC ACGGTAATTC ATTACGTGCA TTGGTGAAGT ATTTGGATAA CATTTCTGAA GAAGAAATTG TTGAATTGAA TATTCCAACT GGTGTCCCAT TGGTCTATGA ATTGGATGAA AACTTGAAGC CAATTAAGCA TTATTACTTG GGTGATCAAG AATTAATTCA ACAAAAGATT AACAGTGTTG CAAGTCAAGC CAGCAAGAAG AAGTGAGTAA GATAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAAT TCTTGAAGAC GAAAGGGCCT CGTGATACGC CTATTTTTAT AGGTTAATGT CATGATAATA ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT GTTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GCAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCTGATGC GGTATTTTCT CCTTACGCAT CTGTGCGGTA TTTCACACCG CATATATGGT GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATACA CTCCGCTATC GCTACGTGAC TGGGTCATGG CTGCGCCCCG ACACCCGCCA ACACCCGCTG ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG CATCCGCTTA CAGACAAGCT GTGACCGTCT CCGGGAGCTG CATGTGTCAG AGGTTTTCAC CGTCATCACC GAAACGCGCG AGGCAGCTGC GGTAAAGCTC ATCAGCGTGG TCGTGAAGCG ATTCACAGAT GTCTGCCTGT TCATCCGCGT CCAGCTCGTT GAGTTTCTCC AGAAGCGTTA ATGTCTGGCT TCTGATAAAG CGGGCCATGT TAAGGGCGGT TTTTTCCTGT TTGGTCACTG ATGCCTCCGT GTAAGGGGGA TTTCTGTTCA TGGGGGTAAT GATACCGATG AAACGAGAGA GGATGCTCAC GATACGGGTT ACTGATGATG AACATGCCCG GTTACTGGAA CGTTGTGAGG GTAAACAACT GGCGGTATGG ATGCGGCGGG ACCAGAGAAA AATCACTCAG GGTCAATGCC AGCGCTTCGT TAATACAGAT GTAGGTGTTC CACAGGGTAG CCAGCAGCAT CCTGCGATGC AGATCCGGAA CATAATGGTG CAGGGCGCTG ACTTCCGCGT TTCCAGACTT TACGAAACAC GGAAACCGAA GACCATTCAT GTTGTTGCTC AGGTCGCAGA CGTTTTGCAG CAGCAGTCGC TTCACGTTCG CTCGCGTATC GGTGATTCAT TCTGCTAACC AGTAAGGCAA CCCCGCCAGC CTAGCCGGGT CCTCAACGAC AGGAGCACGA TCATGCGCAC CCGTGGCCAG GACCCAACGC TGCCCGAGAT GCGCCGCGTG CGGCTGCTGG AGATGGCGGA CGCGATGGAT ATGTTCTGCC AAGGGTTGGT TTGCGCATTC ACAGTTCTCC GCAAGAATTG ATTGGCTCCA ATTCTTGGAG TGGTGAATCC GTTAGCGAGG TGCCGCCGGC TTCCATTCAG GTCGAGGTGG CCCGGCTCCA TGCACCGCGA CGCAACGCGG GGAGGCAGAC AAGGTATAGG GCGGCGCCTA CAATCCATGC CAACCCGTTC CATGTGCTCG CCGAGGCGGC ATAAATCGCC GTGACGATCA GCGGTCCAGT GATCGAAGTT AGGCTGGTAA GAGCCGCGAG CGATCCTTGA AGCTGTCCCT GATGGTCGTC ATCTACCTGC CTGGACAGCA TGGCCTGCAA CGCGGGCATC CCGATGCCGC CGGAAGCGAG AAGAATCATA ATGGGGAAGG CCATCCAGCC TCGCGTCGCG AACGCCAGCA AGACGTAGCC CAGCGCGTCG GCCGCCATGC CGGCGATAAT GGCCTGCTTC TCGCCGAAAC GTTTGGTGGC GGGACCAGTG ACGAAGGCTT GAGCGAGGGC GTGCAAGATT CCGAATACCG CAAGCGACAG GCCGATCATC GTCGCGCTCC AGCGAAAGCG GTCCTCGCCG AAAATGACCC AGAGCGCTGC CGGCACCTGT CCTACGAGTT GCATGATAAA GAAGACAGTC ATAAGTGCGG CGACGATAGT CATGCCCCGC GCCCACCGGA AGGAGCTGAC TGGGTTGAAG GCTCTCAAGG GCATCGGTCG ACGCTCTCCC TTATGCGACT CCTGCATTAG GAAGCAGCCC AGTAGTAGGT TGAGGCCGTT GAGCACCGCC GCCGCAAGGA ATGGTGCATG CAAGGAGATG GCGCCCAACA GTCCCCCGGC CACGGGGCCT GCCACCATAC CCACGCCGAA ACAAGCGCTC ATGAGCCCGA AGTGGCGAGC CCGATCTTCC CCATCGGTGA TGTCGGCGAT ATAGGCGCCA GCAACCGCAC CTGTGGCGCC GGTGATGCCG GCCACGATGC GTCCGGCGTA GAGGATCGAG ATCTCGATCC CGCGAAAT