NafoA.00888.a

adenylosuccinate synthetase isozyme 2-like

CENTER ID:	NafoA.00888.a
ORGANISM:	Naegleria fowleri ATCC 30863
ASSOCIATED DISEASE:
CURRENT STATUS:	diffraction
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIB

Ordering Clones & Proteins

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Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
NafoA.00888.a.B1.GE40392	Full length(NafoA.00888.a)		1	460

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
NafoA.00888.a.B1.PS38134	Full length(NafoA.00888.a)		1	460

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	AmoebaDB:NF0105300

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSQTMNFNNK YTQPKGTVRA VIGGQWGDEG KGKLVDLMAR QHQIVCRYNG GSNAGHSIVD SNGHRYATNL LPSGVLTPGC INLIGNGVVL EVPKLLSEID NLKEKNKKHK GDDSATIDQE FQDIEKRLLI SDRAHLVFGF HKAIDGFQEG VLQETGSQIG TTKRGIGPTY TTKAARSGLR SGDLLTDFAV FEKKYMTLLK TLKSQYASCV ELQNYDAQGE LALYRDVYTP RVHPMIIDTI TYLHKAIYVD GLDILLEGAN AVMLDIDFGT YPFVTSSSPC IGGACTGLGI PPRAISETYG VIKAYCTRVG AGPFPTELKD EVGDTIRKVG GEFGTTTGRP RSCGWLDVVA VKYGCMINGF TALCLTKLDV LDQLEEVKIG VAYKLDGTEI DTVPYSADDM ARVEVVYETF PGWKSDISKV RTFEELPENA QKFVKRIQEL IGVPIKWIGT GAGRDSMICL

NT Sequence

ATGAGCCAAA CAATGAACTT CAATAACAAG TACACCCAGC CAAAAGGAAC TGTTCGAGCA GTGATTGGAG GACAATGGGG TGATGAAGGT AAGGGAAAGT TGGTCGATTT GATGGCCCGA CAGCATCAAA TTGTATGCCG ATACAATGGT GGAAGTAATG CTGGCCATTC CATTGTTGAT TCCAATGGCC ACAGATATGC CACCAATTTA CTCCCTTCTG GCGTTCTCAC TCCCGGATGC ATCAATCTCA TTGGTAATGG TGTTGTTTTG GAGGTACCAA AACTCTTATC TGAAATTGAC AATTTGAAAG AAAAGAACAA GAAACACAAA GGTGATGATT CTGCCACTAT TGACCAAGAG TTCCAAGATA TTGAGAAGCG TTTATTAATT TCCGACCGTG CACATCTTGT TTTCGGTTTC CACAAGGCCA TTGATGGCTT CCAAGAAGGA GTTTTACAAG AAACTGGATC ACAAATTGGA ACGACAAAGC GAGGAATTGG TCCAACCTAC ACCACCAAAG CAGCTCGAAG TGGGTTGCGT AGCGGTGATT TGTTGACCGA TTTTGCAGTT TTCGAGAAAA AGTACATGAC CTTATTGAAG ACATTGAAGA GTCAGTATGC ATCATGTGTC GAATTGCAAA ATTATGATGC ACAAGGAGAA TTGGCTCTCT ACCGAGATGT TTACACTCCC CGAGTTCATC CAATGATTAT TGATACCATT ACTTACCTTC ATAAGGCCAT CTATGTCGAT GGACTTGACA TTTTACTTGA GGGAGCGAAC GCAGTCATGT TGGATATTGA CTTTGGAACT TATCCATTTG TGACATCTTC TTCACCTTGC ATTGGAGGCG CTTGTACTGG TTTGGGCATT CCACCTCGTG CCATTTCTGA AACTTATGGT GTCATTAAGG CATACTGCAC ACGTGTCGGT GCAGGTCCAT TCCCAACTGA GTTGAAGGAT GAAGTTGGAG ACACCATTAG AAAGGTTGGT GGTGAATTTG GTACCACCAC TGGAAGACCA AGAAGTTGCG GATGGCTCGA TGTTGTTGCT GTCAAGTACG GTTGCATGAT TAATGGTTTT ACTGCATTGT GCTTGACCAA ATTGGATGTT TTGGATCAAT TGGAGGAAGT TAAAATCGGT GTTGCCTACA AGTTAGATGG CACTGAGATT GACACTGTTC CATATAGCGC TGATGATATG GCCAGAGTGG AAGTTGTCTA TGAGACCTTC CCAGGCTGGA AGAGTGATAT CTCCAAGGTT CGCACCTTTG AAGAATTGCC TGAAAATGCA CAAAAGTTTG TGAAGCGAAT TCAAGAATTG ATTGGTGTAC CAATTAAATG GATTGGTACA GGAGCTGGAC GTGACTCGAT GATTTGCCTT

Details for NafoA.00888.a.B1.GE40392

HARVESTED ON:	7/15/2016
SEQUENCED ON:	7/18/2016
EXPECTED MW:	51kDa
OBSERVED MW:	51kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMS QTMNFNNKYT QPKGTVRAVI GGQWGDEGKG KLVDLMARQH QIVCRYNGGS NAGHSIVDSN GHRYATNLLP SGVLTPGCIN LIGNGVVLEV PKLLSEIDNL KEKNKKHKGD DSATIDQEFQ DIEKRLLISD RAHLVFGFHK AIDGFQEGVL QETGSQIGTT KRGIGPTYTT KAARSGLRSG DLLTDFAVFE KKYMTLLKTL KSQYASCVEL QNYDAQGELA LYRDVYTPRV HPMIIDTITY LHKAIYVDGL DILLEGANAV MLDIDFGTYP FVTSSSPCIG GACTGLGIPP RAISETYGVI KAYCTRVGAG PFPTELKDEV GDTIRKVGGE FGTTTGRPRS CGWLDVVAVK YGCMINGFTA LCLTKLDVLD QLEEVKIGVA YKLDGTEIDT VPYSADDMAR VEVVYETFPG WKSDISKVRT FEELPENAQK FVKRIQELIG VPIKWIGTGA GRDSMICL

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagcc aaacaatgaa cttcaataac aagtacaccc agccaaaagg aactgttcga gcagtgattg gaggacaatg gggtgatgaa ggtaagggaa agttggtcga tttgatggcc cgacagcatc aaattgtatg ccgatacaat ggtggaagta atgctggcca ttccattgtt gattccaatg gccacagata tgccaccaat ttactccctt ctggcgttct cactcccgga tgcatcaatc tcattggtaa tggtgttgtt ttggaggtac caaaactctt atctgaaatt gacaatttga aagaaaagaa caagaaacac aaaggtgatg attctgccac tattgaccaa gagttccaag atattgagaa gcgtttatta atttccgacc gtgcacatct tgttttcggt ttccacaagg ccattgatgg cttccaagaa ggagttttac aagaaactgg atcacaaatt ggaacgacaa agcgaggaat tggtccaacc tacaccacca aagcagctcg aagtgggttg cgtagcggtg atttgttgac cgattttgca gttttcgaga aaaagtacat gaccttattg aagacattga agagtcagta tgcatcatgt gtcgaattgc aaaattatga tgcacaagga gaattggctc tctaccgaga tgtttacact ccccgagttc atccaatgat tattgatacc attacttacc ttcataaggc catctatgtc gatggacttg acattttact tgagggagcg aacgcagtca tgttggatat tgactttgga acttatccat ttgtgacatc ttcttcacct tgcattggag gcgcttgtac tggtttgggc attccacctc gtgccatttc tgaaacttat ggtgtcatta aggcatactg cacacgtgtc ggtgcaggtc cattcccaac tgagttgaag gatgaagttg gagacaccat tagaaaggtt ggtggtgaat ttggtaccac cactggaaga ccaagaagtt gcggatggct cgatgttgtt gctgtcaagt acggttgcat gattaatggt tttactgcat tgtgcttgac caaattggat gttttggatc aattggagga agttaaaatc ggtgttgcct acaagttaga tggcactgag attgacactg ttccatatag cgctgatgat atggccagag tggaagttgt ctatgagacc ttcccaggct ggaagagtga tatctccaag gttcgcacct ttgaagaatt gcctgaaaat gcacaaaagt ttgtgaagcg aattcaagaa ttgattggtg taccaattaa atggattggt acaggagctg gacgtgactc gatgatttgc ctttgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat

Details for NafoA.00888.a.B1.PS38134

PURIFICATION DATe:	12/1/2016
CONCENTRATION:	49.85mg/ml
OBSERVED MW:	47kDa
EXPRESSION LEVEL:	High Expression
PROTEIN PURIFICATION BUFFER:	SEC Buffer
EXPRESSION HOST:	BL 21 (DE3) Rosetta
VIAL COUNT (approx.):	7
VIAL VOLUME:	200µl
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Protocol Notes

notes unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAGCC AAACAATGAA CTTCAATAAC AAGTACACCC AGCCAAAAGG AACTGTTCGA GCAGTGATTG GAGGACAATG GGGTGATGAA GGTAAGGGAA AGTTGGTCGA TTTGATGGCC CGACAGCATC AAATTGTATG CCGATACAAT GGTGGAAGTA ATGCTGGCCA TTCCATTGTT GATTCCAATG GCCACAGATA TGCCACCAAT TTACTCCCTT CTGGCGTTCT CACTCCCGGA TGCATCAATC TCATTGGTAA TGGTGTTGTT TTGGAGGTAC CAAAACTCTT ATCTGAAATT GACAATTTGA AAGAAAAGAA CAAGAAACAC AAAGGTGATG ATTCTGCCAC TATTGACCAA GAGTTCCAAG ATATTGAGAA GCGTTTATTA ATTTCCGACC GTGCACATCT TGTTTTCGGT TTCCACAAGG CCATTGATGG CTTCCAAGAA GGAGTTTTAC AAGAAACTGG ATCACAAATT GGAACGACAA AGCGAGGAAT TGGTCCAACC TACACCACCA AAGCAGCTCG AAGTGGGTTG CGTAGCGGTG ATTTGTTGAC CGATTTTGCA GTTTTCGAGA AAAAGTACAT GACCTTATTG AAGACATTGA AGAGTCAGTA TGCATCATGT GTCGAATTGC AAAATTATGA TGCACAAGGA GAATTGGCTC TCTACCGAGA TGTTTACACT CCCCGAGTTC ATCCAATGAT TATTGATACC ATTACTTACC TTCATAAGGC CATCTATGTC GATGGACTTG ACATTTTACT TGAGGGAGCG AACGCAGTCA TGTTGGATAT TGACTTTGGA ACTTATCCAT TTGTGACATC TTCTTCACCT TGCATTGGAG GCGCTTGTAC TGGTTTGGGC ATTCCACCTC GTGCCATTTC TGAAACTTAT GGTGTCATTA AGGCATACTG CACACGTGTC GGTGCAGGTC CATTCCCAAC TGAGTTGAAG GATGAAGTTG GAGACACCAT TAGAAAGGTT GGTGGTGAAT TTGGTACCAC CACTGGAAGA CCAAGAAGTT GCGGATGGCT CGATGTTGTT GCTGTCAAGT ACGGTTGCAT GATTAATGGT TTTACTGCAT TGTGCTTGAC CAAATTGGAT GTTTTGGATC AATTGGAGGA AGTTAAAATC GGTGTTGCCT ACAAGTTAGA TGGCACTGAG ATTGACACTG TTCCATATAG CGCTGATGAT ATGGCCAGAG TGGAAGTTGT CTATGAGACC TTCCCAGGCT GGAAGAGTGA TATCTCCAAG GTTCGCACCT TTGAAGAATT GCCTGAAAAT GCACAAAAGT TTGTGAAGCG AATTCAAGAA TTGATTGGTG TACCAATTAA ATGGATTGGT ACAGGAGCTG GACGTGACTC GATGATTTGC CTTTGAGTAA GATAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAAT TCTTGAAGAC GAAAGGGCCT CGTGATACGC CTATTTTTAT AGGTTAATGT CATGATAATA ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT GTTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GCAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCTGATGC GGTATTTTCT CCTTACGCAT CTGTGCGGTA TTTCACACCG CATATATGGT GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATACA CTCCGCTATC GCTACGTGAC TGGGTCATGG CTGCGCCCCG ACACCCGCCA ACACCCGCTG ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG CATCCGCTTA CAGACAAGCT GTGACCGTCT CCGGGAGCTG CATGTGTCAG AGGTTTTCAC CGTCATCACC GAAACGCGCG AGGCAGCTGC GGTAAAGCTC ATCAGCGTGG TCGTGAAGCG ATTCACAGAT GTCTGCCTGT TCATCCGCGT CCAGCTCGTT GAGTTTCTCC AGAAGCGTTA ATGTCTGGCT TCTGATAAAG CGGGCCATGT TAAGGGCGGT TTTTTCCTGT TTGGTCACTG ATGCCTCCGT GTAAGGGGGA TTTCTGTTCA TGGGGGTAAT GATACCGATG AAACGAGAGA GGATGCTCAC GATACGGGTT ACTGATGATG AACATGCCCG GTTACTGGAA CGTTGTGAGG GTAAACAACT GGCGGTATGG ATGCGGCGGG ACCAGAGAAA AATCACTCAG GGTCAATGCC AGCGCTTCGT TAATACAGAT GTAGGTGTTC CACAGGGTAG CCAGCAGCAT CCTGCGATGC AGATCCGGAA CATAATGGTG CAGGGCGCTG ACTTCCGCGT TTCCAGACTT TACGAAACAC GGAAACCGAA GACCATTCAT GTTGTTGCTC AGGTCGCAGA CGTTTTGCAG CAGCAGTCGC TTCACGTTCG CTCGCGTATC GGTGATTCAT TCTGCTAACC AGTAAGGCAA CCCCGCCAGC CTAGCCGGGT CCTCAACGAC AGGAGCACGA TCATGCGCAC CCGTGGCCAG GACCCAACGC TGCCCGAGAT GCGCCGCGTG CGGCTGCTGG AGATGGCGGA CGCGATGGAT ATGTTCTGCC AAGGGTTGGT TTGCGCATTC ACAGTTCTCC GCAAGAATTG ATTGGCTCCA ATTCTTGGAG TGGTGAATCC GTTAGCGAGG TGCCGCCGGC TTCCATTCAG GTCGAGGTGG CCCGGCTCCA TGCACCGCGA CGCAACGCGG GGAGGCAGAC AAGGTATAGG GCGGCGCCTA CAATCCATGC CAACCCGTTC CATGTGCTCG CCGAGGCGGC ATAAATCGCC GTGACGATCA GCGGTCCAGT GATCGAAGTT AGGCTGGTAA GAGCCGCGAG CGATCCTTGA AGCTGTCCCT GATGGTCGTC ATCTACCTGC CTGGACAGCA TGGCCTGCAA CGCGGGCATC CCGATGCCGC CGGAAGCGAG AAGAATCATA ATGGGGAAGG CCATCCAGCC TCGCGTCGCG AACGCCAGCA AGACGTAGCC CAGCGCGTCG GCCGCCATGC CGGCGATAAT GGCCTGCTTC TCGCCGAAAC GTTTGGTGGC GGGACCAGTG ACGAAGGCTT GAGCGAGGGC GTGCAAGATT CCGAATACCG CAAGCGACAG GCCGATCATC GTCGCGCTCC AGCGAAAGCG GTCCTCGCCG AAAATGACCC AGAGCGCTGC CGGCACCTGT CCTACGAGTT GCATGATAAA GAAGACAGTC ATAAGTGCGG CGACGATAGT CATGCCCCGC GCCCACCGGA AGGAGCTGAC TGGGTTGAAG GCTCTCAAGG GCATCGGTCG ACGCTCTCCC TTATGCGACT CCTGCATTAG GAAGCAGCCC AGTAGTAGGT TGAGGCCGTT GAGCACCGCC GCCGCAAGGA ATGGTGCATG CAAGGAGATG GCGCCCAACA GTCCCCCGGC CACGGGGCCT GCCACCATAC CCACGCCGAA ACAAGCGCTC ATGAGCCCGA AGTGGCGAGC CCGATCTTCC CCATCGGTGA TGTCGGCGAT ATAGGCGCCA GCAACCGCAC CTGTGGCGCC GGTGATGCCG GCCACGATGC GTCCGGCGTA GAGGATCGAG ATCTCGATCC CGCGAAAT