NafoA.00879.b

actin 7 isoform 1

CENTER ID: NafoA.00879.b
ORGANISM: Naegleria fowleri ATCC 30863
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIB

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
NafoA.00879.b.B1.GE40391 Full length(NafoA.00879.b) 1 363
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
External Resources
RESOURCE REFERENCE ID
EuPathDB: AmoebaDB:NF0121260
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLEPSRNFKF EKKQMCDYQC IVMDNGSFMT KAGFAGDPSP LCVFPTIVGR PLQQRSNMSY GMKKTPCFVG DEPSRKGPVQ WDCPLSRGVI HNFQDMEIIW RHTFVNELRV EDPFVVLLSE TPLHPKANRE KTTQIMFESF QVRALFVCNQ AVLALFAHSG RTTGMVLDSG ESVSHFVPVY EGHALKHAVT RVEMGGNEVT LQLCKLLGER GFSFRTSGVE KELVREIKQK LCDVMPSLLD FELEMKKHDK EDSFISNNPR STMGKSYELP DGRILTIGSE RFRCTEILFQ PSLLDSEMVS EKSCSSPEGL HEALVHSISK CDLECRREFY SNIILSGGNT LFPGMPERIQ QELVQMAPPS HAD
NT Sequence
ATGTTGGAAC CATCAAGGAA CTTTAAATTT GAAAAAAAAC AAATGTGTGA CTACCAGTGC ATCGTCATGG ACAATGGAAG CTTCATGACC AAAGCCGGAT TCGCTGGAGA CCCATCTCCT CTTTGTGTCT TTCCAACCAT TGTCGGAAGA CCTCTCCAAC AACGATCGAA CATGTCGTAT GGAATGAAAA AAACACCTTG CTTTGTTGGA GATGAACCAA GTCGAAAGGG TCCGGTACAA TGGGATTGTC CTCTTTCTCG TGGCGTTATT CACAATTTCC AAGATATGGA AATCATTTGG CGACACACCT TTGTCAACGA GTTACGAGTG GAAGATCCGT TTGTGGTGCT TCTCTCCGAA ACTCCTCTCC ATCCAAAAGC GAATCGAGAG AAAACCACTC AAATCATGTT CGAGTCCTTT CAGGTGAGAG CTCTCTTTGT TTGCAATCAG GCTGTTCTTG CATTGTTTGC ACATTCGGGA CGGACCACAG GAATGGTGTT GGATTCCGGA GAGAGTGTTT CCCATTTCGT TCCGGTCTAT GAAGGTCATG CTTTGAAACA TGCCGTGACA CGAGTGGAGA TGGGTGGCAA TGAGGTGACT CTTCAGTTAT GCAAGTTATT AGGGGAACGA GGTTTTTCCT TTCGAACCAG TGGTGTAGAA AAGGAACTTG TGAGGGAAAT CAAGCAAAAA TTGTGTGATG TGATGCCCTC CTTGTTGGAT TTTGAATTGG AAATGAAGAA GCACGACAAG GAGGACAGTT TCATTTCAAA TAATCCTCGT TCAACAATGG GAAAATCATA TGAATTACCG GATGGAAGAA TTCTCACGAT TGGAAGTGAA AGATTTCGTT GCACTGAAAT ATTATTTCAA CCTTCTCTCT TGGATTCAGA AATGGTGAGT GAAAAAAGCT GCAGTTCACC TGAAGGTTTG CATGAAGCCC TTGTTCATTC CATTTCCAAA TGTGATCTTG AATGTAGAAG AGAGTTCTAT TCGAATATCA TCTTATCTGG TGGAAATACA CTGTTTCCAG GAATGCCAGA GAGAATACAG CAAGAATTGG TTCAAATGGC TCCTCCTTCC CATGCAGAT
Details for NafoA.00879.b.B1.GE40391
HARVESTED ON: 7/15/2016
SEQUENCED ON: 7/18/2016
EXPECTED MW: 42kDa
OBSERVED MW: 42kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Good (10-50)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHML EPSRNFKFEK KQMCDYQCIV MDNGSFMTKA GFAGDPSPLC VFPTIVGRPL QQRSNMSYGM KKTPCFVGDE PSRKGPVQWD CPLSRGVIHN FQDMEIIWRH TFVNELRVED PFVVLLSETP LHPKANREKT TQIMFESFQV RALFVCNQAV LALFAHSGRT TGMVLDSGES VSHFVPVYEG HALKHAVTRV EMGGNEVTLQ LCKLLGERGF SFRTSGVEKE LVREIKQKLC DVMPSLLDFE LEMKKHDKED SFISNNPRST MGKSYELPDG RILTIGSERF RCTEILFQPS LLDSEMVSEK SCSSPEGLHE ALVHSISKCD LECRREFYSN IILSGGNTLF PGMPERIQQE LVQMAPPSHA D
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgttgg aaccatcaag gaactttaaa tttgaaaaaa aacaaatgtg tgactaccag tgcatcgtca tggacaatgg aagcttcatg accaaagccg gattcgctgg agacccatct cctctttgtg tctttccaac cattgtcgga agacctctcc aacaacgatc gaacatgtcg tatggaatga aaaaaacacc ttgctttgtt ggagatgaac caagtcgaaa gggtccggta caatgggatt gtcctctttc tcgtggcgtt attcacaatt tccaagatat ggaaatcatt tggcgacaca cctttgtcaa cgagttacga gtggaagatc cgtttgtggt gcttctctcc gaaactcctc tccatccaaa agcgaatcga gagaaaacca ctcaaatcat gttcgagtcc tttcaggtga gagctctctt tgtttgcaat caggctgttc ttgcattgtt tgcacattcg ggacggacca caggaatggt gttggattcc ggagagagtg tttcccattt cgttccggtc tatgaaggtc atgctttgaa acatgccgtg acacgagtgg agatgggtgg caatgaggtg actcttcagt tatgcaagtt attaggggaa cgaggttttt cctttcgaac cagtggtgta gaaaaggaac ttgtgaggga aatcaagcaa aaattgtgtg atgtgatgcc ctccttgttg gattttgaat tggaaatgaa gaagcacgac aaggaggaca gtttcatttc aaataatcct cgttcaacaa tgggaaaatc atatgaatta ccggatggaa gaattctcac gattggaagt gaaagatttc gttgcactga aatattattt caaccttctc tcttggattc agaaatggtg agtgaaaaaa gctgcagttc acctgaaggt ttgcatgaag cccttgttca ttccatttcc aaatgtgatc ttgaatgtag aagagagttc tattcgaata tcatcttatc tggtggaaat acactgtttc caggaatgcc agagagaata cagcaagaat tggttcaaat ggctcctcct tcccatgcag attgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat