NafoA.00879.a

actin-like protein

CENTER ID:	NafoA.00879.a
ORGANISM:	Naegleria fowleri ATCC 30863
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
NafoA.00879.a.B1.GE40390	Full length(NafoA.00879.a)		1	422

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	AmoebaDB:NF0002440

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSYSNDVIAR QNPVLVIDNG TGFTKMGYAG NSEPNYIIPT CISHNPKSLD KGLEDLDFYI GYEAQEKQGG NYDLVYPMRH GLIEDWSKME MLWEQCFFNY LKCEPEDHYL MLTEPPLNPP ENREYTAEIM FETFNVSGLY IAVQAVLALC ASWTSSKSKK LGLGGTLSGT VIDSGDGVTH VIPVVDGYVI GSSIKHIPLA GRDVTEFLMR FIMDREKNIP SEDIKLAVQK IKEQHCYVCS DLAKEFNKFD TNPAENILKY SQENKKYGKW EIDVAYERFL APEVFFNPEI FSADFSTPLP DVVDHCIQSS PIDARRVLYR NIVLSGGSTM FEHFDRRLQR DLKKKLDDRY EKAVSMTSSG KEKPKPLECN VIAHSHQRFA VWFGGSMLGT MAEFKRVCYT KAQYDEMGPS IARRSQVFQS FE

NT Sequence

ATGAGCTACT CAAACGACGT GATTGCCAGA CAAAACCCAG TCCTCGTCAT TGACAATGGT ACTGGTTTTA CCAAGATGGG CTATGCAGGT AATAGTGAGC CAAACTACAT CATCCCAACC TGTATTTCAC ACAATCCAAA ATCTCTTGAT AAAGGTCTGG AGGATTTGGA CTTTTACATT GGATACGAAG CACAAGAAAA GCAAGGAGGA AACTATGACT TGGTTTATCC AATGAGACAC GGTTTGATCG AAGATTGGAG CAAGATGGAA ATGTTGTGGG AACAATGCTT CTTCAACTAT TTGAAGTGTG AGCCTGAGGA TCACTACTTG ATGTTAACAG AACCTCCTCT CAATCCTCCA GAAAACCGTG AATATACTGC TGAAATCATG TTCGAAACAT TCAACGTGTC CGGATTGTAC ATTGCCGTTC AAGCTGTTTT GGCTTTGTGT GCATCATGGA CATCTTCCAA GTCAAAGAAG CTTGGTTTGG GTGGTACTTT GTCTGGTACT GTCATTGACT CTGGTGATGG TGTTACTCAC GTCATTCCTG TTGTAGATGG TTATGTCATT GGTTCTAGTA TTAAGCACAT TCCATTGGCC GGTAGAGATG TCACCGAGTT CTTGATGCGT TTCATCATGG ACCGTGAAAA GAACATTCCT TCCGAAGACA TCAAGTTGGC TGTTCAAAAG ATCAAGGAAC AACATTGTTA CGTATGCTCT GATTTGGCAA AGGAGTTTAA CAAGTTTGAC ACAAACCCAG CTGAAAACAT TCTCAAATAT TCACAAGAAA ATAAGAAGTA TGGTAAATGG GAAATTGATG TTGCCTATGA GAGATTCTTG GCTCCTGAAG TCTTCTTCAA TCCAGAAATT TTCTCTGCTG ACTTCAGTAC ACCTCTCCCT GATGTTGTTG ACCACTGCAT TCAATCTTCA CCAATTGATG CTCGTAGAGT GTTGTACAGG AATATTGTCC TTTCCGGTGG TTCTACCATG TTTGAACATT TTGACAGACG TTTGCAACGT GACTTGAAGA AGAAATTGGA TGATCGTTAT GAAAAGGCTG TCTCAATGAC CTCATCTGGT AAAGAGAAAC CAAAGCCATT AGAATGTAAC GTTATTGCTC ACTCACACCA ACGTTTTGCT GTTTGGTTTG GTGGTTCCAT GTTGGGTACT ATGGCTGAAT TTAAGAGAGT TTGTTACACA AAGGCTCAAT ACGATGAGAT GGGTCCATCC ATTGCTCGTA GATCTCAAGT CTTCCAAAGC TTTGAA

Details for NafoA.00879.a.B1.GE40390

HARVESTED ON:	7/15/2016
SEQUENCED ON:	7/18/2016
EXPECTED MW:	49kDa
OBSERVED MW:	49kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMS YSNDVIARQN PVLVIDNGTG FTKMGYAGNS EPNYIIPTCI SHNPKSLDKG LEDLDFYIGY EAQEKQGGNY DLVYPMRHGL IEDWSKMEML WEQCFFNYLK CEPEDHYLML TEPPLNPPEN REYTAEIMFE TFNVSGLYIA VQAVLALCAS WTSSKSKKLG LGGTLSGTVI DSGDGVTHVI PVVDGYVIGS SIKHIPLAGR DVTEFLMRFI MDREKNIPSE DIKLAVQKIK EQHCYVCSDL AKEFNKFDTN PAENILKYSQ ENKKYGKWEI DVAYERFLAP EVFFNPEIFS ADFSTPLPDV VDHCIQSSPI DARRVLYRNI VLSGGSTMFE HFDRRLQRDL KKKLDDRYEK AVSMTSSGKE KPKPLECNVI AHSHQRFAVW FGGSMLGTMA EFKRVCYTKA QYDEMGPSIA RRSQVFQSFE

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagct actcaaacga cgtgattgcc agacaaaacc cagtcctcgt cattgacaat ggtactggtt ttaccaagat gggctatgca ggtaatagtg agccaaacta catcatccca acctgtattt cacacaatcc aaaatctctt gataaaggtc tggaggattt ggacttttac attggatacg aagcacaaga aaagcaagga ggaaactatg acttggttta tccaatgaga cacggtttga tcgaagattg gagcaagatg gaaatgttgt gggaacaatg cttcttcaac tatttgaagt gtgagcctga ggatcactac ttgatgttaa cagaacctcc tctcaatcct ccagaaaacc gtgaatatac tgctgaaatc atgttcgaaa cattcaacgt gtccggattg tacattgccg ttcaagctgt tttggctttg tgtgcatcat ggacatcttc caagtcaaag aagcttggtt tgggtggtac tttgtctggt actgtcattg actctggtga tggtgttact cacgtcattc ctgttgtaga tggttatgtc attggttcta gtattaagca cattccattg gccggtagag atgtcaccga gttcttgatg cgtttcatca tggaccgtga aaagaacatt ccttccgaag acatcaagtt ggctgttcaa aagatcaagg aacaacattg ttacgtatgc tctgatttgg caaaggagtt taacaagttt gacacaaacc cagctgaaaa cattctcaaa tattcacaag aaaataagaa gtatggtaaa tgggaaattg atgttgccta tgagagattc ttggctcctg aagtcttctt caatccagaa attttctctg ctgacttcag tacacctctc cctgatgttg ttgaccactg cattcaatct tcaccaattg atgctcgtag agtgttgtac aggaatattg tcctttccgg tggttctacc atgtttgaac attttgacag acgtttgcaa cgtgacttga agaagaaatt ggatgatcgt tatgaaaagg ctgtctcaat gacctcatct ggtaaagaga aaccaaagcc attagaatgt aacgttattg ctcactcaca ccaacgtttt gctgtttggt ttggtggttc catgttgggt actatggctg aatttaagag agtttgttac acaaaggctc aatacgatga gatgggtcca tccattgctc gtagatctca agtcttccaa agctttgaat gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat