NafoA.00859.a

26s protease regulatory subunit 6b homolog

CENTER ID:	NafoA.00859.a
ORGANISM:	Naegleria fowleri ATCC 30863
ASSOCIATED DISEASE:
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
NafoA.00859.a.B1.GE40389	Full length(NafoA.00859.a)		1	431

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	AmoebaDB:NF0022530

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MDELGLGKKK QSPGGADQMD VDEVSAQAQA TEQQDELSVL LKPLQELSEQ DLYKKLKHIE SELEFHDIQE EYVKYEQKNL KRELLRAKEE VKRIKSVPLV IGQFLEMIDQ NTGIISSTTG SSSHVRILST IDRELLKPNA SVALHRHSSA LVEVLPPEAD SSIQLVGANE RPDVTYADIG GLDIQKQEIR EAVELPLTHF GLYEQIGIDP PRGVLLYGPP GTGKTMLAKA VAHHTTAAFI RVVGSEFVQK YLGEGPRMVR DVFRLARENA PAIVFIDEVD SIATKRFDAQ TGADREVQRI LLELLTQMDG FEQTTNVKVI MATNRWDTLD PALLRPGRLD RKIEFPLPDR RQKRLVFQVC TSKMNLAEDV DLEDYVSRPD KISCAEIAAI CQEAGMQAVR KNRYVVTAKD FEKGYKTHVR KTDGDHEYLL R

NT Sequence

ATGGACGAAT TAGGCCTCGG AAAGAAGAAA CAATCACCAG GCGGTGCTGA TCAAATGGAT GTTGATGAAG TCAGCGCTCA AGCACAAGCT ACCGAACAAC AGGATGAATT GAGCGTGCTT CTCAAACCTC TTCAAGAACT TTCCGAACAA GATCTCTACA AAAAGCTTAA ACATATTGAG AGTGAGCTTG AATTTCATGA TATCCAAGAA GAATATGTCA AGTACGAACA AAAGAACTTG AAAAGAGAAT TATTAAGAGC CAAAGAGGAG GTGAAACGAA TCAAATCAGT ACCTCTCGTA ATTGGACAAT TCTTGGAAAT GATTGATCAA AATACTGGAA TTATTTCTTC AACAACTGGC TCATCGAGTC ATGTTCGAAT CTTGAGCACG ATTGACAGAG AATTATTGAA GCCCAATGCT TCAGTTGCCC TACACAGACA TTCAAGTGCT TTGGTAGAGG TTTTACCCCC TGAAGCAGAC AGTAGTATTC AATTGGTTGG AGCTAATGAA CGACCTGATG TTACCTACGC AGATATTGGA GGTTTAGATA TTCAAAAACA AGAAATTAGA GAGGCCGTTG AGCTTCCACT GACACACTTT GGATTATATG AACAAATTGG TATCGATCCA CCAAGAGGTG TTTTATTGTA TGGACCTCCT GGTACTGGAA AGACCATGTT GGCCAAAGCA GTTGCCCACC ATACCACTGC TGCTTTCATT AGAGTAGTTG GTTCAGAATT TGTACAAAAG TATCTCGGTG AAGGTCCAAG AATGGTTCGT GACGTGTTCA GATTGGCTCG TGAAAATGCA CCTGCTATTG TTTTCATTGA CGAAGTCGAT TCAATTGCTA CCAAACGTTT TGATGCTCAA ACAGGAGCCG ATCGTGAGGT ACAACGTATT TTGCTCGAGT TGTTGACACA AATGGATGGT TTCGAACAAA CTACCAATGT CAAAGTGATC ATGGCTACCA ATAGATGGGA TACTTTGGAT CCTGCTTTGC TTCGTCCAGG TCGTTTGGAT AGAAAGATTG AGTTCCCACT ACCAGACCGT CGACAAAAAC GTTTGGTATT CCAAGTTTGT ACCAGTAAGA TGAATTTAGC AGAAGACGTT GATTTGGAAG ATTATGTTTC CCGTCCTGAC AAGATATCAT GTGCTGAGAT TGCAGCGATT TGTCAAGAGG CAGGTATGCA AGCTGTGAGA AAGAACAGAT ATGTCGTCAC GGCCAAAGAT TTTGAAAAGG GCTACAAGAC CCACGTGAGA AAGACTGATG GCGATCATGA ATACTTGTTA CGT

Details for NafoA.00859.a.B1.GE40389

HARVESTED ON:	7/15/2016
SEQUENCED ON:	7/18/2016
EXPECTED MW:	50kDa
OBSERVED MW:	50kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Insoluble
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMD ELGLGKKKQS PGGADQMDVD EVSAQAQATE QQDELSVLLK PLQELSEQDL YKKLKHIESE LEFHDIQEEY VKYEQKNLKR ELLRAKEEVK RIKSVPLVIG QFLEMIDQNT GIISSTTGSS SHVRILSTID RELLKPNASV ALHRHSSALV EVLPPEADSS IQLVGANERP DVTYADIGGL DIQKQEIREA VELPLTHFGL YEQIGIDPPR GVLLYGPPGT GKTMLAKAVA HHTTAAFIRV VGSEFVQKYL GEGPRMVRDV FRLARENAPA IVFIDEVDSI ATKRFDAQTG ADREVQRILL ELLTQMDGFE QTTNVKVIMA TNRWDTLDPA LLRPGRLDRK IEFPLPDRRQ KRLVFQVCTS KMNLAEDVDL EDYVSRPDKI SCAEIAAICQ EAGMQAVRKN RYVVTAKDFE KGYKTHVRKT DGDHEYLLR

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggacg aattaggcct cggaaagaag aaacaatcac caggcggtgc tgatcaaatg gatgttgatg aagtcagcgc tcaagcacaa gctaccgaac aacaggatga attgagcgtg cttctcaaac ctcttcaaga actttccgaa caagatctct acaaaaagct taaacatatt gagagtgagc ttgaatttca tgatatccaa gaagaatatg tcaagtacga acaaaagaac ttgaaaagag aattattaag agccaaagag gaggtgaaac gaatcaaatc agtacctctc gtaattggac aattcttgga aatgattgat caaaatactg gaattatttc ttcaacaact ggctcatcga gtcatgttcg aatcttgagc acgattgaca gagaattatt gaagcccaat gcttcagttg ccctacacag acattcaagt gctttggtag aggttttacc ccctgaagca gacagtagta ttcaattggt tggagctaat gaacgacctg atgttaccta cgcagatatt ggaggtttag atattcaaaa acaagaaatt agagaggccg ttgagcttcc actgacacac tttggattat atgaacaaat tggtatcgat ccaccaagag gtgttttatt gtatggacct cctggtactg gaaagaccat gttggccaaa gcagttgccc accataccac tgctgctttc attagagtag ttggttcaga atttgtacaa aagtatctcg gtgaaggtcc aagaatggtt cgtgacgtgt tcagattggc tcgtgaaaat gcacctgcta ttgttttcat tgacgaagtc gattcaattg ctaccaaacg ttttgatgct caaacaggag ccgatcgtga ggtacaacgt attttgctcg agttgttgac acaaatggat ggtttcgaac aaactaccaa tgtcaaagtg atcatggcta ccaatagatg ggatactttg gatcctgctt tgcttcgtcc aggtcgtttg gatagaaaga ttgagttccc actaccagac cgtcgacaaa aacgtttggt attccaagtt tgtaccagta agatgaattt agcagaagac gttgatttgg aagattatgt ttcccgtcct gacaagatat catgtgctga gattgcagcg atttgtcaag aggcaggtat gcaagctgtg agaaagaaca gatatgtcgt cacggccaaa gattttgaaa agggctacaa gacccacgtg agaaagactg atggcgatca tgaatacttg ttacgttgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t