NafoA.00855.a
glyceraldehyde-3-phosphate dehydrogenase
| CENTER ID: | NafoA.00855.a |
| ORGANISM: | Naegleria fowleri ATCC 30863 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| NafoA.00855.a.B1.GE40388 | Full length(NafoA.00855.a) | 1 | 333 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| NafoA.00855.a.B1.PS38303 | Full length(NafoA.00855.a) | 1 | 333 |
Structures
6NLX
DEPOSITED: 1/9/2019
DETERMINATION: XRay
CLONE: NafoA.00855.a.B1.GE40388
PROTEIN: NafoA.00855.a.B1.PS38303
Publications by SSGCID
Naegleria fowleri: Protein structures to facilitate drug discovery for the deadly, pathogenic free-living amoeba
Abendroth J, Barrett LK, Dranow DM, Edwards TE, Myler PJ, Phan IQ, Staker B, Subramanian S, Tran N, Van Voorhis WC, Craig JC, Smith C, Chun IKH, Kyle D, Barrett KF, Morris JC, Tillery L, Rice CA, Goldstein J, Lassner JW, Xu L, Young RM, Osterhout B, Leahy JW, Manetsch R
PLoS ONE - 2021
volume 16, issue 3, pages e0241738
PMID: 33760815; PMCID: PMC7990177
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| EuPathDB: | AmoebaDB:NF0055660 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MVKVGINGFG RIGRLVFRAS LERTDVEVVA INDIMMTPDY MIYMIKYDSV HGKFNGKLEY TENSIKVNGR EIHVFCEREP EKLPWGQYGV EYVVESTGVF TKLDTASKHL KGGAKRVVIS APADTPTFVM GVNNHEFKPE MTVINNASCT TNCLAPIAAV LHENFGIVEG LMTTVHALTA TQPTVDAPSK KDWRGGRAAG YNIIPSSTGA AKAVGLVIPS LNGKLTGMAF RVPTADVSVV DLTCRLEKPA TKKQIDEAMK KASESERFKG ILKYTEEEVV SSDFIHDSAS STYDSKASIS LNDHFVKVVA WYDNEWGYSN RVLDLIISTS KVQ
NT Sequence
ATGGTCAAGG TTGGAATCAA CGGCTTTGGT AGAATTGGTA GACTTGTCTT CCGTGCTTCA TTGGAAAGAA CCGATGTTGA AGTGGTTGCC ATCAACGATA TTATGATGAC TCCTGATTAC ATGATCTACA TGATCAAGTA TGATTCTGTC CACGGTAAAT TCAATGGTAA ACTCGAATAC ACTGAAAACT CAATCAAGGT GAACGGACGT GAAATTCATG TCTTCTGTGA GAGAGAACCA GAGAAGTTGC CTTGGGGCCA ATACGGAGTT GAATATGTTG TTGAATCCAC TGGTGTCTTT ACCAAATTGG ACACTGCTTC AAAGCACTTG AAGGGTGGTG CTAAGAGAGT TGTGATTTCT GCTCCAGCTG ATACTCCAAC TTTTGTTATG GGAGTCAATA ATCATGAATT CAAGCCAGAA ATGACTGTGA TCAACAATGC TTCCTGCACA ACCAATTGCT TGGCTCCAAT TGCTGCAGTC TTGCACGAAA ACTTTGGAAT TGTTGAAGGC TTGATGACCA CTGTCCACGC CTTGACTGCC ACCCAACCAA CAGTTGATGC TCCATCCAAG AAGGATTGGA GAGGTGGTCG TGCTGCTGGA TACAACATTA TTCCATCATC CACTGGTGCT GCTAAGGCTG TTGGTTTGGT CATTCCATCA TTGAACGGAA AGTTGACTGG TATGGCATTC AGAGTCCCAA CTGCTGATGT TTCTGTTGTC GATTTGACTT GCAGATTGGA GAAGCCTGCT ACCAAGAAGC AAATTGATGA AGCAATGAAG AAGGCTTCCG AGTCCGAGAG ATTCAAGGGT ATTTTGAAGT ACACTGAAGA GGAAGTTGTT TCAAGTGACT TTATTCATGA TAGTGCTTCA TCTACTTATG ACAGCAAGGC CAGTATCTCA TTGAACGATC ACTTTGTTAA GGTTGTTGCT TGGTATGACA ATGAATGGGG TTACTCTAAC AGAGTTTTGG ACTTGATTAT TTCAACTTCA AAGGTCCAA
Details for NafoA.00855.a.B1.GE40388
| HARVESTED ON: | 7/15/2016 |
| SEQUENCED ON: | 7/18/2016 |
| EXPECTED MW: | 38kDa |
| OBSERVED MW: | 38kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Low Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMV KVGINGFGRI GRLVFRASLE RTDVEVVAIN DIMMTPDYMI YMIKYDSVHG KFNGKLEYTE NSIKVNGREI HVFCEREPEK LPWGQYGVEY VVESTGVFTK LDTASKHLKG GAKRVVISAP ADTPTFVMGV NNHEFKPEMT VINNASCTTN CLAPIAAVLH ENFGIVEGLM TTVHALTATQ PTVDAPSKKD WRGGRAAGYN IIPSSTGAAK AVGLVIPSLN GKLTGMAFRV PTADVSVVDL TCRLEKPATK KQIDEAMKKA SESERFKGIL KYTEEEVVSS DFIHDSASST YDSKASISLN DHFVKVVAWY DNEWGYSNRV LDLIXXTSKV Q
Validated NT Sequence
ttactcactt attggacctt tgaagttnnn nnaatcaagt ccaaaactct gttagagtaa ccccattcat tgtcatacca agcaacaacc ttaacaaagt gatcgttcaa tgagatactg gccttgctgt cataagtaga tgaagcacta tcatgaataa agtcacttga aacaacttcc tcttcagtgt acttcaaaat acccttgaat ctctcggact cggaagcctt cttcattgct tcatcaattt gcttcttggt agcaggcttc tccaatctgc aagtcaaatc gacaacagaa acatcagcag ttgggactct gaatgccata ccagtcaact ttccgttcaa tgatggaatg accaaaccaa cagccttagc agcaccagtg gatgatggaa taatgttgta tccagcagca cgaccacctc tccaatcctt cttggatgga gcatcaactg ttggttgggt ggcagtcaag gcgtggacag tggtcatcaa gccttcaaca attccaaagt tttcgtgcaa gactgcagca attggagcca agcaattggt tgtgcaggaa gcattgttga tcacagtcat ttctggcttg aattcatgat tattgactcc cataacaaaa gttggagtat cagctggagc agaaatcaca actctcttag caccaccctt caagtgcttt gaagcagtgt ccaatttggt aaagacacca gtggattcaa caacatattc aactccgtat tggccccaag gcaacttctc tggttctctc tcacagaaga catgaatttc acgtccgttc accttgattg agttttcagt gtattcgagt ttaccattga atttaccgtg gacagaatca tacttgatca tgtagatcat gtaatcagga gtcatcataa tatcgttgat ggcaaccact tcaacatcgg ttctttccaa tgaagcacgg aagacaagtc taccaattct accaaagccg ttgattccaa ccttgaccat atggtggtgg tggtggtgag ccat
Expected Protein Sequence
MAHHHHHHMV KVGINGFGRI GRLVFRASLE RTDVEVVAIN DIMMTPDYMI YMIKYDSVHG KFNGKLEYTE NSIKVNGREI HVFCEREPEK LPWGQYGVEY VVESTGVFTK LDTASKHLKG GAKRVVISAP ADTPTFVMGV NNHEFKPEMT VINNASCTTN CLAPIAAVLH ENFGIVEGLM TTVHALTATQ PTVDAPSKKD WRGGRAAGYN IIPSSTGAAK AVGLVIPSLN GKLTGMAFRV PTADVSVVDL TCRLEKPATK KQIDEAMKKA SESERFKGIL KYTEEEVVSS DFIHDSASST YDSKASISLN DHFVKVVAWY DNEWGYSNRV LDLIISTSKV Q
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggtca aggttggaat caacggcttt ggtagaattg gtagacttgt cttccgtgct tcattggaaa gaaccgatgt tgaagtggtt gccatcaacg atattatgat gactcctgat tacatgatct acatgatcaa gtatgattct gtccacggta aattcaatgg taaactcgaa tacactgaaa actcaatcaa ggtgaacgga cgtgaaattc atgtcttctg tgagagagaa ccagagaagt tgccttgggg ccaatacgga gttgaatatg ttgttgaatc cactggtgtc tttaccaaat tggacactgc ttcaaagcac ttgaagggtg gtgctaagag agttgtgatt tctgctccag ctgatactcc aacttttgtt atgggagtca ataatcatga attcaagcca gaaatgactg tgatcaacaa tgcttcctgc acaaccaatt gcttggctcc aattgctgca gtcttgcacg aaaactttgg aattgttgaa ggcttgatga ccactgtcca cgccttgact gccacccaac caacagttga tgctccatcc aagaaggatt ggagaggtgg tcgtgctgct ggatacaaca ttattccatc atccactggt gctgctaagg ctgttggttt ggtcattcca tcattgaacg gaaagttgac tggtatggca ttcagagtcc caactgctga tgtttctgtt gtcgatttga cttgcagatt ggagaagcct gctaccaaga agcaaattga tgaagcaatg aagaaggctt ccgagtccga gagattcaag ggtattttga agtacactga agaggaagtt gtttcaagtg actttattca tgatagtgct tcatctactt atgacagcaa ggccagtatc tcattgaacg atcactttgt taaggttgtt gcttggtatg acaatgaatg gggttactct aacagagttt tggacttgat tatttcaact tcaaaggtcc aatgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat
Details for NafoA.00855.a.B1.PS38303
| PURIFICATION DATe: | 6/7/2017 |
| CONCENTRATION: | 42.8mg/ml |
| OBSERVED MW: | 37kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | SEC: 20 mM HEPES pH 7.0, 300 mM NaCl, 5% glycerol, 1 mM TCEP |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| VIAL COUNT (approx.): | 11 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMV KVGINGFGRI GRLVFRASLE RTDVEVVAIN DIMMTPDYMI YMIKYDSVHG KFNGKLEYTE NSIKVNGREI HVFCEREPEK LPWGQYGVEY VVESTGVFTK LDTASKHLKG GAKRVVISAP ADTPTFVMGV NNHEFKPEMT VINNASCTTN CLAPIAAVLH ENFGIVEGLM TTVHALTATQ PTVDAPSKKD WRGGRAAGYN IIPSSTGAAK AVGLVIPSLN GKLTGMAFRV PTADVSVVDL TCRLEKPATK KQIDEAMKKA SESERFKGIL KYTEEEVVSS DFIHDSASST YDSKASISLN DHFVKVVAWY DNEWGYSNRV LDLIXXTSKV Q
Validated NT Sequence
ttactcactt attggacctt tgaagttnnn nnaatcaagt ccaaaactct gttagagtaa ccccattcat tgtcatacca agcaacaacc ttaacaaagt gatcgttcaa tgagatactg gccttgctgt cataagtaga tgaagcacta tcatgaataa agtcacttga aacaacttcc tcttcagtgt acttcaaaat acccttgaat ctctcggact cggaagcctt cttcattgct tcatcaattt gcttcttggt agcaggcttc tccaatctgc aagtcaaatc gacaacagaa acatcagcag ttgggactct gaatgccata ccagtcaact ttccgttcaa tgatggaatg accaaaccaa cagccttagc agcaccagtg gatgatggaa taatgttgta tccagcagca cgaccacctc tccaatcctt cttggatgga gcatcaactg ttggttgggt ggcagtcaag gcgtggacag tggtcatcaa gccttcaaca attccaaagt tttcgtgcaa gactgcagca attggagcca agcaattggt tgtgcaggaa gcattgttga tcacagtcat ttctggcttg aattcatgat tattgactcc cataacaaaa gttggagtat cagctggagc agaaatcaca actctcttag caccaccctt caagtgcttt gaagcagtgt ccaatttggt aaagacacca gtggattcaa caacatattc aactccgtat tggccccaag gcaacttctc tggttctctc tcacagaaga catgaatttc acgtccgttc accttgattg agttttcagt gtattcgagt ttaccattga atttaccgtg gacagaatca tacttgatca tgtagatcat gtaatcagga gtcatcataa tatcgttgat ggcaaccact tcaacatcgg ttctttccaa tgaagcacgg aagacaagtc taccaattct accaaagccg ttgattccaa ccttgaccat atggtggtgg tggtggtgag ccat
Expressed Protein Sequence
MAHHHHHHMV KVGINGFGRI GRLVFRASLE RTDVEVVAIN DIMMTPDYMI YMIKYDSVHG KFNGKLEYTE NSIKVNGREI HVFCEREPEK LPWGQYGVEY VVESTGVFTK LDTASKHLKG GAKRVVISAP ADTPTFVMGV NNHEFKPEMT VINNASCTTN CLAPIAAVLH ENFGIVEGLM TTVHALTATQ PTVDAPSKKD WRGGRAAGYN IIPSSTGAAK AVGLVIPSLN GKLTGMAFRV PTADVSVVDL TCRLEKPATK KQIDEAMKKA SESERFKGIL KYTEEEVVSS DFIHDSASST YDSKASISLN DHFVKVVAWY DNEWGYSNRV LDLIISTSKV Q
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGTCA AGGTTGGAAT CAACGGCTTT GGTAGAATTG GTAGACTTGT CTTCCGTGCT TCATTGGAAA GAACCGATGT TGAAGTGGTT GCCATCAACG ATATTATGAT GACTCCTGAT TACATGATCT ACATGATCAA GTATGATTCT GTCCACGGTA AATTCAATGG TAAACTCGAA TACACTGAAA ACTCAATCAA GGTGAACGGA CGTGAAATTC ATGTCTTCTG TGAGAGAGAA CCAGAGAAGT TGCCTTGGGG CCAATACGGA GTTGAATATG TTGTTGAATC CACTGGTGTC TTTACCAAAT TGGACACTGC TTCAAAGCAC TTGAAGGGTG GTGCTAAGAG AGTTGTGATT TCTGCTCCAG CTGATACTCC AACTTTTGTT ATGGGAGTCA ATAATCATGA ATTCAAGCCA GAAATGACTG TGATCAACAA TGCTTCCTGC ACAACCAATT GCTTGGCTCC AATTGCTGCA GTCTTGCACG AAAACTTTGG AATTGTTGAA GGCTTGATGA CCACTGTCCA CGCCTTGACT GCCACCCAAC CAACAGTTGA TGCTCCATCC AAGAAGGATT GGAGAGGTGG TCGTGCTGCT GGATACAACA TTATTCCATC ATCCACTGGT GCTGCTAAGG CTGTTGGTTT GGTCATTCCA TCATTGAACG GAAAGTTGAC TGGTATGGCA TTCAGAGTCC CAACTGCTGA TGTTTCTGTT GTCGATTTGA CTTGCAGATT GGAGAAGCCT GCTACCAAGA AGCAAATTGA TGAAGCAATG AAGAAGGCTT CCGAGTCCGA GAGATTCAAG GGTATTTTGA AGTACACTGA AGAGGAAGTT GTTTCAAGTG ACTTTATTCA TGATAGTGCT TCATCTACTT ATGACAGCAA GGCCAGTATC TCATTGAACG ATCACTTTGT TAAGGTTGTT GCTTGGTATG ACAATGAATG GGGTTACTCT AACAGAGTTT TGGACTTGAT TATTTCAACT TCAAAGGTCC AATGAGTAAG ATAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAATT CTTGAAGACG AAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTG TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG CAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCTGATGCG GTATTTTCTC CTTACGCATC TGTGCGGTAT TTCACACCGC ATATATGGTG CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGTATACAC TCCGCTATCG CTACGTGACT GGGTCATGGC TGCGCCCCGA CACCCGCCAA CACCCGCTGA CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC ATCCGCTTAC AGACAAGCTG TGACCGTCTC CGGGAGCTGC ATGTGTCAGA GGTTTTCACC GTCATCACCG AAACGCGCGA GGCAGCTGCG GTAAAGCTCA TCAGCGTGGT CGTGAAGCGA TTCACAGATG TCTGCCTGTT CATCCGCGTC CAGCTCGTTG AGTTTCTCCA GAAGCGTTAA TGTCTGGCTT CTGATAAAGC GGGCCATGTT AAGGGCGGTT TTTTCCTGTT TGGTCACTGA TGCCTCCGTG TAAGGGGGAT TTCTGTTCAT GGGGGTAATG ATACCGATGA AACGAGAGAG GATGCTCACG ATACGGGTTA CTGATGATGA ACATGCCCGG TTACTGGAAC GTTGTGAGGG TAAACAACTG GCGGTATGGA TGCGGCGGGA CCAGAGAAAA ATCACTCAGG GTCAATGCCA GCGCTTCGTT AATACAGATG TAGGTGTTCC ACAGGGTAGC CAGCAGCATC CTGCGATGCA GATCCGGAAC ATAATGGTGC AGGGCGCTGA CTTCCGCGTT TCCAGACTTT ACGAAACACG GAAACCGAAG ACCATTCATG TTGTTGCTCA GGTCGCAGAC GTTTTGCAGC AGCAGTCGCT TCACGTTCGC TCGCGTATCG GTGATTCATT CTGCTAACCA GTAAGGCAAC CCCGCCAGCC TAGCCGGGTC CTCAACGACA GGAGCACGAT CATGCGCACC CGTGGCCAGG ACCCAACGCT GCCCGAGATG CGCCGCGTGC GGCTGCTGGA GATGGCGGAC GCGATGGATA TGTTCTGCCA AGGGTTGGTT TGCGCATTCA CAGTTCTCCG CAAGAATTGA TTGGCTCCAA TTCTTGGAGT GGTGAATCCG TTAGCGAGGT GCCGCCGGCT TCCATTCAGG TCGAGGTGGC CCGGCTCCAT GCACCGCGAC GCAACGCGGG GAGGCAGACA AGGTATAGGG CGGCGCCTAC AATCCATGCC AACCCGTTCC ATGTGCTCGC CGAGGCGGCA TAAATCGCCG TGACGATCAG CGGTCCAGTG ATCGAAGTTA GGCTGGTAAG AGCCGCGAGC GATCCTTGAA GCTGTCCCTG ATGGTCGTCA TCTACCTGCC TGGACAGCAT GGCCTGCAAC GCGGGCATCC CGATGCCGCC GGAAGCGAGA AGAATCATAA TGGGGAAGGC CATCCAGCCT CGCGTCGCGA ACGCCAGCAA GACGTAGCCC AGCGCGTCGG CCGCCATGCC GGCGATAATG GCCTGCTTCT CGCCGAAACG TTTGGTGGCG GGACCAGTGA CGAAGGCTTG AGCGAGGGCG TGCAAGATTC CGAATACCGC AAGCGACAGG CCGATCATCG TCGCGCTCCA GCGAAAGCGG TCCTCGCCGA AAATGACCCA GAGCGCTGCC GGCACCTGTC CTACGAGTTG CATGATAAAG AAGACAGTCA TAAGTGCGGC GACGATAGTC ATGCCCCGCG CCCACCGGAA GGAGCTGACT GGGTTGAAGG CTCTCAAGGG CATCGGTCGA CGCTCTCCCT TATGCGACTC CTGCATTAGG AAGCAGCCCA GTAGTAGGTT GAGGCCGTTG AGCACCGCCG CCGCAAGGAA TGGTGCATGC AAGGAGATGG CGCCCAACAG TCCCCCGGCC ACGGGGCCTG CCACCATACC CACGCCGAAA CAAGCGCTCA TGAGCCCGAA GTGGCGAGCC CGATCTTCCC CATCGGTGAT GTCGGCGATA TAGGCGCCAG CAACCGCACC TGTGGCGCCG GTGATGCCGG CCACGATGCG TCCGGCGTAG AGGATCGAGA TCTCGATCCC GCGAAAT