NafoA.00825.a

asparagine--trna cytoplasmic-like

CENTER ID:	NafoA.00825.a
ORGANISM:	Naegleria fowleri ATCC 30863
ASSOCIATED DISEASE:
CURRENT STATUS:	diffraction
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
NafoA.00825.a.B1.GE40317	Full length(NafoA.00825.a)		1	593

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
NafoA.00825.a.B1.PS38156	Full length(NafoA.00825.a)		1	593

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	AmoebaDB:NF0018560

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MSSSAEQLPL SLLITFFTEA NKRLQEEKEA SLKEEWSEEQ EKTIEALLNG IVEKAKQQAS YTGADVNETS LREAGFAQGG ESEELCSLVT KFFEVRPMAK SQSKKVSKRV EKMQKEREKS KEKEKQQQQK LEEAKKISFQ EDTSLPEAKH IKIRDVGPQY EGQRVMIYGW CHRIRVQGKG LLFITLRDGT GLLQTVLSGV LCQTYEALTL HLESSVMIKG TLKADPRAPM GFELQADFWK LIGTSPESLP FNEEVKDPVI LLDQRHLILR GEYTSAILKI RSAVLQSFRE HYYSRGYTEI TPPTLVQTQV EGGSTLFKFD YFGEDAFLTQ SSQLYLETVI PALGDSFCIA QSYRAEKSDT PRHLSEFTHV EAERPFISFE DLLNTLEDLV CDVTERVWAK CKDLVLKVNP DAQKLKDCLK RPFKRMTYAD CIQFCRDNQV YKDEETKELF EFGDDITDAP ERKMLEKIGE PVLMIKFPAN MKAFYMKRCE DDNSLTESVD LLLPGVGEVI GGSMRLESFD TLMEGYKKEG ISAEPYYWYS DQRKYGTCPH GGYGLGLERY MLWLLREEDE EKRRVKTVRQ TCLYPRYMGR CRP

NT Sequence

ATGAGTTCAT CAGCTGAACA ACTCCCACTT TCCTTGTTGA TTACCTTCTT TACCGAGGCC AACAAGAGAC TCCAAGAGGA AAAGGAAGCA TCATTAAAAG AAGAATGGAG TGAAGAACAA GAAAAAACCA TTGAAGCCCT TCTCAATGGT ATTGTCGAAA AGGCAAAGCA ACAAGCTAGC TATACCGGTG CCGATGTGAA CGAAACCTCG CTGAGAGAAG CCGGATTTGC TCAAGGAGGC GAATCTGAAG AGCTCTGCTC CCTCGTTACA AAGTTCTTTG AAGTCAGACC AATGGCCAAG TCTCAATCAA AGAAGGTGTC CAAGAGAGTG GAAAAGATGC AAAAGGAAAG AGAAAAGTCA AAAGAAAAGG AAAAACAACA ACAACAAAAA CTCGAAGAAG CCAAGAAAAT TTCATTCCAA GAAGATACAT CACTTCCAGA GGCTAAACAT ATTAAAATCA GAGATGTCGG ACCACAATAT GAGGGACAAC GTGTGATGAT TTATGGTTGG TGTCATAGAA TTAGAGTCCA AGGAAAAGGA CTTTTATTCA TCACTCTCAG AGATGGTACA GGCCTACTCC AAACTGTATT GAGTGGAGTT TTGTGTCAAA CCTATGAAGC TTTAACTCTT CATTTGGAAT CATCTGTAAT GATCAAAGGT ACATTGAAGG CAGATCCAAG AGCTCCAATG GGCTTTGAAT TGCAAGCTGA TTTCTGGAAA TTGATCGGAA CATCACCAGA AAGTCTTCCA TTCAACGAAG AAGTGAAAGA TCCAGTGATT TTGCTCGACC AAAGACACTT GATTTTAAGA GGAGAATATA CTTCAGCTAT CTTAAAGATT CGTTCAGCAG TTTTACAAAG CTTCCGTGAA CATTATTATT CACGTGGTTA TACTGAAATC ACTCCTCCAA CTCTTGTTCA AACTCAAGTT GAAGGTGGTT CAACACTTTT CAAATTTGAT TACTTTGGTG AGGATGCGTT TTTGACTCAA TCAAGTCAAT TATATTTGGA AACAGTCATT CCTGCATTAG GAGACAGCTT CTGTATTGCT CAATCGTATC GTGCAGAGAA GTCTGACACT CCACGTCACT TGAGCGAATT TACTCATGTT GAGGCAGAGA GACCATTCAT TTCTTTTGAG GATTTATTGA ATACTTTGGA AGATTTAGTT TGTGACGTTA CAGAAAGAGT TTGGGCAAAG TGCAAGGATT TGGTATTGAA GGTCAACCCT GACGCACAAA AACTTAAGGA TTGTTTGAAA CGCCCATTCA AACGCATGAC TTATGCTGAT TGTATTCAAT TCTGCAGAGA CAATCAAGTT TACAAAGATG AGGAAACAAA GGAGCTCTTT GAATTTGGTG ATGACATTAC TGATGCACCA GAAAGAAAGA TGTTGGAAAA AATTGGTGAA CCAGTCCTCA TGATTAAATT CCCAGCTAAC ATGAAGGCTT TCTACATGAA GAGATGTGAA GATGACAACT CGCTGACTGA ATCTGTTGAC TTGTTGCTTC CAGGTGTTGG TGAAGTTATT GGTGGAAGTA TGCGTCTCGA ATCCTTCGAT ACTCTCATGG AAGGATATAA GAAGGAGGGA ATTAGTGCTG AACCATATTA CTGGTATAGT GACCAAAGAA AGTATGGCAC TTGTCCTCAT GGTGGTTATG GTTTAGGTCT TGAAAGATAC ATGCTGTGGT TGTTGAGAGA AGAAGACGAA GAAAAGAGAA GAGTCAAGAC AGTAAGACAA ACATGCTTGT ATCCACGTTA TATGGGTAGA TGTCGACCT

Details for NafoA.00825.a.B1.GE40317

HARVESTED ON:	6/13/2016
SEQUENCED ON:	6/16/2016
EXPECTED MW:	69kDa
OBSERVED MW:	70kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expected Protein Sequence

MAHHHHHHMS SSAEQLPLSL LITFFTEANK RLQEEKEASL KEEWSEEQEK TIEALLNGIV EKAKQQASYT GADVNETSLR EAGFAQGGES EELCSLVTKF FEVRPMAKSQ SKKVSKRVEK MQKEREKSKE KEKQQQQKLE EAKKISFQED TSLPEAKHIK IRDVGPQYEG QRVMIYGWCH RIRVQGKGLL FITLRDGTGL LQTVLSGVLC QTYEALTLHL ESSVMIKGTL KADPRAPMGF ELQADFWKLI GTSPESLPFN EEVKDPVILL DQRHLILRGE YTSAILKIRS AVLQSFREHY YSRGYTEITP PTLVQTQVEG GSTLFKFDYF GEDAFLTQSS QLYLETVIPA LGDSFCIAQS YRAEKSDTPR HLSEFTHVEA ERPFISFEDL LNTLEDLVCD VTERVWAKCK DLVLKVNPDA QKLKDCLKRP FKRMTYADCI QFCRDNQVYK DEETKELFEF GDDITDAPER KMLEKIGEPV LMIKFPANMK AFYMKRCEDD NSLTESVDLL LPGVGEVIGG SMRLESFDTL MEGYKKEGIS AEPYYWYSDQ RKYGTCPHGG YGLGLERYML WLLREEDEEK RRVKTVRQTC LYPRYMGRCR P

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagtt catcagctga acaactccca ctttccttgt tgattacctt ctttaccgag gccaacaaga gactccaaga ggaaaaggaa gcatcattaa aagaagaatg gagtgaagaa caagaaaaaa ccattgaagc ccttctcaat ggtattgtcg aaaaggcaaa gcaacaagct agctataccg gtgccgatgt gaacgaaacc tcgctgagag aagccggatt tgctcaagga ggcgaatctg aagagctctg ctccctcgtt acaaagttct ttgaagtcag accaatggcc aagtctcaat caaagaaggt gtccaagaga gtggaaaaga tgcaaaagga aagagaaaag tcaaaagaaa aggaaaaaca acaacaacaa aaactcgaag aagccaagaa aatttcattc caagaagata catcacttcc agaggctaaa catattaaaa tcagagatgt cggaccacaa tatgagggac aacgtgtgat gatttatggt tggtgtcata gaattagagt ccaaggaaaa ggacttttat tcatcactct cagagatggt acaggcctac tccaaactgt attgagtgga gttttgtgtc aaacctatga agctttaact cttcatttgg aatcatctgt aatgatcaaa ggtacattga aggcagatcc aagagctcca atgggctttg aattgcaagc tgatttctgg aaattgatcg gaacatcacc agaaagtctt ccattcaacg aagaagtgaa agatccagtg attttgctcg accaaagaca cttgatttta agaggagaat atacttcagc tatcttaaag attcgttcag cagttttaca aagcttccgt gaacattatt attcacgtgg ttatactgaa atcactcctc caactcttgt tcaaactcaa gttgaaggtg gttcaacact tttcaaattt gattactttg gtgaggatgc gtttttgact caatcaagtc aattatattt ggaaacagtc attcctgcat taggagacag cttctgtatt gctcaatcgt atcgtgcaga gaagtctgac actccacgtc acttgagcga atttactcat gttgaggcag agagaccatt catttctttt gaggatttat tgaatacttt ggaagattta gtttgtgacg ttacagaaag agtttgggca aagtgcaagg atttggtatt gaaggtcaac cctgacgcac aaaaacttaa ggattgtttg aaacgcccat tcaaacgcat gacttatgct gattgtattc aattctgcag agacaatcaa gtttacaaag atgaggaaac aaaggagctc tttgaatttg gtgatgacat tactgatgca ccagaaagaa agatgttgga aaaaattggt gaaccagtcc tcatgattaa attcccagct aacatgaagg ctttctacat gaagagatgt gaagatgaca actcgctgac tgaatctgtt gacttgttgc ttccaggtgt tggtgaagtt attggtggaa gtatgcgtct cgaatccttc gatactctca tggaaggata taagaaggag ggaattagtg ctgaaccata ttactggtat agtgaccaaa gaaagtatgg cacttgtcct catggtggtt atggtttagg tcttgaaaga tacatgctgt ggttgttgag agaagaagac gaagaaaaga gaagagtcaa gacagtaaga caaacatgct tgtatccacg ttatatgggt agatgtcgac cttgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat

Details for NafoA.00825.a.B1.PS38156

PURIFICATION DATe:	12/20/2016
CONCENTRATION:	24.98mg/ml
OBSERVED MW:	70kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	SEC buffer
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	2
VIAL VOLUME:	110µl
PERCENT IDENTITY:	data unavailable
PERCENT COVERAGE:	data unavailable

Protocol Notes

notes unavailable

Validated AA Sequence

sequence unavailable

Validated NT Sequence

sequence unavailable

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAGTT CATCAGCTGA ACAACTCCCA CTTTCCTTGT TGATTACCTT CTTTACCGAG GCCAACAAGA GACTCCAAGA GGAAAAGGAA GCATCATTAA AAGAAGAATG GAGTGAAGAA CAAGAAAAAA CCATTGAAGC CCTTCTCAAT GGTATTGTCG AAAAGGCAAA GCAACAAGCT AGCTATACCG GTGCCGATGT GAACGAAACC TCGCTGAGAG AAGCCGGATT TGCTCAAGGA GGCGAATCTG AAGAGCTCTG CTCCCTCGTT ACAAAGTTCT TTGAAGTCAG ACCAATGGCC AAGTCTCAAT CAAAGAAGGT GTCCAAGAGA GTGGAAAAGA TGCAAAAGGA AAGAGAAAAG TCAAAAGAAA AGGAAAAACA ACAACAACAA AAACTCGAAG AAGCCAAGAA AATTTCATTC CAAGAAGATA CATCACTTCC AGAGGCTAAA CATATTAAAA TCAGAGATGT CGGACCACAA TATGAGGGAC AACGTGTGAT GATTTATGGT TGGTGTCATA GAATTAGAGT CCAAGGAAAA GGACTTTTAT TCATCACTCT CAGAGATGGT ACAGGCCTAC TCCAAACTGT ATTGAGTGGA GTTTTGTGTC AAACCTATGA AGCTTTAACT CTTCATTTGG AATCATCTGT AATGATCAAA GGTACATTGA AGGCAGATCC AAGAGCTCCA ATGGGCTTTG AATTGCAAGC TGATTTCTGG AAATTGATCG GAACATCACC AGAAAGTCTT CCATTCAACG AAGAAGTGAA AGATCCAGTG ATTTTGCTCG ACCAAAGACA CTTGATTTTA AGAGGAGAAT ATACTTCAGC TATCTTAAAG ATTCGTTCAG CAGTTTTACA AAGCTTCCGT GAACATTATT ATTCACGTGG TTATACTGAA ATCACTCCTC CAACTCTTGT TCAAACTCAA GTTGAAGGTG GTTCAACACT TTTCAAATTT GATTACTTTG GTGAGGATGC GTTTTTGACT CAATCAAGTC AATTATATTT GGAAACAGTC ATTCCTGCAT TAGGAGACAG CTTCTGTATT GCTCAATCGT ATCGTGCAGA GAAGTCTGAC ACTCCACGTC ACTTGAGCGA ATTTACTCAT GTTGAGGCAG AGAGACCATT CATTTCTTTT GAGGATTTAT TGAATACTTT GGAAGATTTA GTTTGTGACG TTACAGAAAG AGTTTGGGCA AAGTGCAAGG ATTTGGTATT GAAGGTCAAC CCTGACGCAC AAAAACTTAA GGATTGTTTG AAACGCCCAT TCAAACGCAT GACTTATGCT GATTGTATTC AATTCTGCAG AGACAATCAA GTTTACAAAG ATGAGGAAAC AAAGGAGCTC TTTGAATTTG GTGATGACAT TACTGATGCA CCAGAAAGAA AGATGTTGGA AAAAATTGGT GAACCAGTCC TCATGATTAA ATTCCCAGCT AACATGAAGG CTTTCTACAT GAAGAGATGT GAAGATGACA ACTCGCTGAC TGAATCTGTT GACTTGTTGC TTCCAGGTGT TGGTGAAGTT ATTGGTGGAA GTATGCGTCT CGAATCCTTC GATACTCTCA TGGAAGGATA TAAGAAGGAG GGAATTAGTG CTGAACCATA TTACTGGTAT AGTGACCAAA GAAAGTATGG CACTTGTCCT CATGGTGGTT ATGGTTTAGG TCTTGAAAGA TACATGCTGT GGTTGTTGAG AGAAGAAGAC GAAGAAAAGA GAAGAGTCAA GACAGTAAGA CAAACATGCT TGTATCCACG TTATATGGGT AGATGTCGAC CTTGAGTAAG ATAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAATT CTTGAAGACG AAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTG TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG CAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCTGATGCG GTATTTTCTC CTTACGCATC TGTGCGGTAT TTCACACCGC ATATATGGTG CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGTATACAC TCCGCTATCG CTACGTGACT GGGTCATGGC TGCGCCCCGA CACCCGCCAA CACCCGCTGA CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC ATCCGCTTAC AGACAAGCTG TGACCGTCTC CGGGAGCTGC ATGTGTCAGA GGTTTTCACC GTCATCACCG AAACGCGCGA GGCAGCTGCG GTAAAGCTCA TCAGCGTGGT CGTGAAGCGA TTCACAGATG TCTGCCTGTT CATCCGCGTC CAGCTCGTTG AGTTTCTCCA GAAGCGTTAA TGTCTGGCTT CTGATAAAGC GGGCCATGTT AAGGGCGGTT TTTTCCTGTT TGGTCACTGA TGCCTCCGTG TAAGGGGGAT TTCTGTTCAT GGGGGTAATG ATACCGATGA AACGAGAGAG GATGCTCACG ATACGGGTTA CTGATGATGA ACATGCCCGG TTACTGGAAC GTTGTGAGGG TAAACAACTG GCGGTATGGA TGCGGCGGGA CCAGAGAAAA ATCACTCAGG GTCAATGCCA GCGCTTCGTT AATACAGATG TAGGTGTTCC ACAGGGTAGC CAGCAGCATC CTGCGATGCA GATCCGGAAC ATAATGGTGC AGGGCGCTGA CTTCCGCGTT TCCAGACTTT ACGAAACACG GAAACCGAAG ACCATTCATG TTGTTGCTCA GGTCGCAGAC GTTTTGCAGC AGCAGTCGCT TCACGTTCGC TCGCGTATCG GTGATTCATT CTGCTAACCA GTAAGGCAAC CCCGCCAGCC TAGCCGGGTC CTCAACGACA GGAGCACGAT CATGCGCACC CGTGGCCAGG ACCCAACGCT GCCCGAGATG CGCCGCGTGC GGCTGCTGGA GATGGCGGAC GCGATGGATA TGTTCTGCCA AGGGTTGGTT TGCGCATTCA CAGTTCTCCG CAAGAATTGA TTGGCTCCAA TTCTTGGAGT GGTGAATCCG TTAGCGAGGT GCCGCCGGCT TCCATTCAGG TCGAGGTGGC CCGGCTCCAT GCACCGCGAC GCAACGCGGG GAGGCAGACA AGGTATAGGG CGGCGCCTAC AATCCATGCC AACCCGTTCC ATGTGCTCGC CGAGGCGGCA TAAATCGCCG TGACGATCAG CGGTCCAGTG ATCGAAGTTA GGCTGGTAAG AGCCGCGAGC GATCCTTGAA GCTGTCCCTG ATGGTCGTCA TCTACCTGCC TGGACAGCAT GGCCTGCAAC GCGGGCATCC CGATGCCGCC GGAAGCGAGA AGAATCATAA TGGGGAAGGC CATCCAGCCT CGCGTCGCGA ACGCCAGCAA GACGTAGCCC AGCGCGTCGG CCGCCATGCC GGCGATAATG GCCTGCTTCT CGCCGAAACG TTTGGTGGCG GGACCAGTGA CGAAGGCTTG AGCGAGGGCG TGCAAGATTC CGAATACCGC AAGCGACAGG CCGATCATCG TCGCGCTCCA GCGAAAGCGG TCCTCGCCGA AAATGACCCA GAGCGCTGCC GGCACCTGTC CTACGAGTTG CATGATAAAG AAGACAGTCA TAAGTGCGGC GACGATAGTC ATGCCCCGCG CCCACCGGAA GGAGCTGACT GGGTTGAAGG CTCTCAAGGG CATCGGTCGA CGCTCTCCCT TATGCGACTC CTGCATTAGG AAGCAGCCCA GTAGTAGGTT GAGGCCGTTG AGCACCGCCG CCGCAAGGAA TGGTGCATGC AAGGAGATGG CGCCCAACAG TCCCCCGGCC ACGGGGCCTG CCACCATACC CACGCCGAAA CAAGCGCTCA TGAGCCCGAA GTGGCGAGCC CGATCTTCCC CATCGGTGAT GTCGGCGATA TAGGCGCCAG CAACCGCACC TGTGGCGCCG GTGATGCCGG CCACGATGCG TCCGGCGTAG AGGATCGAGA TCTCGATCCC GCGAAAT