NafoA.00439.a

casein kinase ii alpha subunit

CENTER ID: NafoA.00439.a
ORGANISM: Naegleria fowleri ATCC 30863
ASSOCIATED DISEASE:
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIB

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
NafoA.00439.a.B2.GE40605 ordered domain 34 322
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
External Resources
RESOURCE REFERENCE ID
EuPathDB: AmoebaDB:NF0001750
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSRARVYADV NLRKPKEYWD YESLQIEWGS QDKYEIVRKV GRGKYSEVFE GINAETNERC IIKVLKPVKK KKIKREIKIL QNLCGGTNIV KLLDVVRDPE SKTPSLIMEN VDNTDFRFLY PTFSDFDIRY YMYELLKALD FCHSMGIMHR DIKPHNVMID HKQKKLRVID WGLAEFYHPG QEYNVRVASR YFKGPELLVD FREYDYSLDM WSLGCMFAGM IFRKEPFFHG RDNYDQLVKI AKVLGTEELY DYLDKYSITL SAHYEGILSR NRYPRKPWHS FVNKDNSHLA NDEAIDFLDK LLRYDHQQRM TAKEAMQHPY ISSIFSKLNE TSQNSTGNMD TRE
NT Sequence
ATGAGCCGTG CGAGAGTGTA CGCTGATGTA AACTTGAGAA AACCGAAGGA ATATTGGGAT TATGAAAGCC TTCAAATTGA GTGGGGTTCT CAAGATAAAT ACGAAATTGT TCGAAAAGTC GGCCGAGGAA AATACAGCGA AGTTTTTGAA GGAATAAACG CCGAGACAAA TGAGCGATGT ATCATCAAAG TACTCAAACC CGTCAAGAAG AAGAAAATCA AGAGAGAAAT TAAAATTCTA CAAAACCTTT GTGGAGGAAC CAACATTGTA AAACTTTTGG ATGTTGTTAG AGATCCTGAA TCCAAAACAC CATCCCTGAT CATGGAAAAT GTTGATAACA CTGATTTTCG TTTTTTATAT CCAACCTTCT CTGATTTTGA TATTAGATAT TACATGTATG AGTTATTGAA GGCTCTAGAT TTTTGCCATT CGATGGGAAT AATGCATCGA GATATTAAAC CACACAATGT CATGATTGAC CACAAACAAA AGAAATTACG TGTCATTGAC TGGGGACTTG CAGAATTCTA TCATCCAGGC CAAGAATACA ATGTAAGAGT GGCTTCAAGA TATTTCAAAG GACCAGAGCT TTTGGTCGAT TTCAGAGAAT ATGATTATTC TCTTGATATG TGGAGTCTTG GATGTATGTT TGCTGGAATG ATTTTCAGAA AGGAACCATT CTTCCATGGT CGTGATAACT ATGACCAACT AGTTAAAATC GCCAAAGTGT TAGGAACTGA GGAATTGTAT GACTATTTGG ACAAGTATTC TATTACACTC TCTGCACACT ACGAGGGCAT TCTTTCAAGA AATAGATATC CAAGAAAGCC ATGGCACTCG TTTGTGAACA AGGACAATTC GCATCTAGCC AACGATGAAG CCATCGACTT TTTGGACAAA TTACTTCGAT ACGATCATCA ACAACGAATG ACAGCTAAGG AGGCCATGCA ACATCCTTAC ATTAGCAGCA TATTTAGCAA ATTGAATGAA ACAAGCCAAA ACTCTACCGG AAATATGGAC ACACGTGAG
Details for NafoA.00439.a.B2.GE40605
HARVESTED ON: 10/18/2016
SEQUENCED ON: 10/19/2016
EXPECTED MW: 35kDa
OBSERVED MW: 35kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMY EIVRKVGRGK YSEVFEGINA ETNERCIIKV LKPVKKKKIK REIKILQNLC GGTNIVKLLD VVRDPESKTP SLIMENVDNT DFRFLYPTFS DFDIRYYMYE LLKALDFCHS MGIMHRDIKP HNVMIDHKQK KLRVIDWGLA EFYHPGQEYN VRVASRYFKG PELLVDFREY DYSLDMWSLG CMFAGMIFRK EPFFHGRDNY DQLVKIAKVL GTEELYDYLD KYSITLSAHY EGILSRNRYP RKPWHSFVNK DNSHLANDEA IDFLDKLLRY DHQQRMTAKE AM
Validated NT Sequence
gcatggcctc cttagctgtc attcgttgtt gatgatcgta tcgaagtaat ttgtccaaaa agtcgatggc ttcatcgttg gctagatgcg aattgtcctt gttcacaaac gagtgccatg gctttcttgg atatctattt cttgaaagaa tgccctcgta gtgtgcagag agtgtaatag aatacttgtc caaatagtca tacaattcct cagttcctaa cactttggcg attttaacta gttggtcata gttatcacga ccatggaaga atggttcctt tctgaaaatc attccagcaa acatacatcc aagactccac atatcaagag aataatcata ttctctgaaa tcgaccaaaa gctctggtcc tttgaaatat cttgaagcca ctcttacatt gtattcttgg cctggatgat agaattctgc aagtccccag tcaatgacac gtaatttctt ttgtttgtgg tcaatcatga cattgtgtgg tttaatatct cgatgcatta ttcccatcga atggcaaaaa tctagagcct tcaataactc atacatgtaa tatctaatat caaaatcaga gaaggttgga tataaaaaac gaaaatcagt gttatcaaca ttttccatga tcagggatgg tgttttggat tcaggatctc taacaacatc caaaagtttt acaatgttgg ttcctccaca aaggttttgt agaattttaa tttctctctt gattttcttc ttcttgacag gtttgagtac tttgatgata catcgctcat ttgtctcggc gtttattcct tcaaaaactt cgctgtattt tcctcggccg acttttcgaa caatttcgta catatggtgg tggtggtggt gagccat
Expected Protein Sequence
MAHHHHHHYE IVRKVGRGKY SEVFEGINAE TNERCIIKVL KPVKKKKIKR EIKILQNLCG GTNIVKLLDV VRDPESKTPS LIMENVDNTD FRFLYPTFSD FDIRYYMYEL LKALDFCHSM GIMHRDIKPH NVMIDHKQKK LRVIDWGLAE FYHPGQEYNV RVASRYFKGP ELLVDFREYD YSLDMWSLGC MFAGMIFRKE PFFHGRDNYD QLVKIAKVLG TEELYDYLDK YSITLSAHYE GILSRNRYPR KPWHSFVNKD NSHLANDEAI DFLDKLLRYD HQQRMTAKEA MQHPYIS
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac cattacgaaa ttgttcgaaa agtcggccga ggaaaataca gcgaagtttt tgaaggaata aacgccgaga caaatgagcg atgtatcatc aaagtactca aacccgtcaa gaagaagaaa atcaagagag aaattaaaat tctacaaaac ctttgtggag gaaccaacat tgtaaaactt ttggatgttg ttagagatcc tgaatccaaa acaccatccc tgatcatgga aaatgttgat aacactgatt ttcgtttttt atatccaacc ttctctgatt ttgatattag atattacatg tatgagttat tgaaggctct agatttttgc cattcgatgg gaataatgca tcgagatatt aaaccacaca atgtcatgat tgaccacaaa caaaagaaat tacgtgtcat tgactgggga cttgcagaat tctatcatcc aggccaagaa tacaatgtaa gagtggcttc aagatatttc aaaggaccag agcttttggt cgatttcaga gaatatgatt attctcttga tatgtggagt cttggatgta tgtttgctgg aatgattttc agaaaggaac cattcttcca tggtcgtgat aactatgacc aactagttaa aatcgccaaa gtgttaggaa ctgaggaatt gtatgactat ttggacaagt attctattac actctctgca cactacgagg gcattctttc aagaaataga tatccaagaa agccatggca ctcgtttgtg aacaaggaca attcgcatct agccaacgat gaagccatcg actttttgga caaattactt cgatacgatc atcaacaacg aatgacagct aaggaggcca tgcaacatcc ttacattagc tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat