NafoA.00379.a
enolase family protein
| CENTER ID: | NafoA.00379.a |
| ORGANISM: | Naegleria fowleri ATCC 30863 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
Materials can be ordered from SSGCID using the button in the "order material" column.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| NafoA.00379.a.B4.GE44253 | ENO2 truncation 2 | 62 | 512 | ||
| NafoA.00379.a.B5.GE44254 | ENO2 truncation 3 | 44 | 512 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| NafoA.00379.a.B4.PW38978 | ENO2 truncation 2 | 62 | 512 | ||
| NafoA.00379.a.B5.PW38980 | ENO2 truncation 3 | 44 | 512 | ||
| NafoA.00379.a.B5.PW39044 | ENO2 truncation 3 | 44 | 512 | ||
| NafoA.00379.a.B5.PW39046 | ENO2 truncation 3 | 44 | 512 | ||
| NafoA.00379.a.B4.PW39047 | ENO2 truncation 2 | 62 | 512 |
Structures
7UGH
DEPOSITED: 3/24/2022
DETERMINATION: XRay
CLONE: NafoA.00379.a.B5.GE44254
PROTEIN: NafoA.00379.a.B5.PW38980
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| EuPathDB: | AmoebaDB:NF0118810 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLYLVLMLVE NGFGQNCFLH SQKICSNMTD KQPVSEYLQN HNLQKLVEDA LNECYNANAS DPVGFLGHFF LNRGKKGAVN RVDKLVGREI LDSRGNPTVE VDVYANGQKR PVATASAPSG ASTGSNEAHE LRDGDKSRYL GKGVLKAVKN VNDVLGKAVE GKSLENLTEL DQALIDADGD ELKSNLGGNA ITACSFALAT AGAAVRNEEL FLYLARAFHG ADKFENLKFR LPTPMVNILN GGKHAGGRLQ IQEFMILPKE NQPFREKVRC VAEVYQHLGK ILAERAGPSA KNVGDEGGFA PNLETADEAL NYIEEAIGKA GYKVGEDVFL ALDAASSEFY NSDTKKYEIT QQKEFLTSEE MVEYYVQLVN RHPAIISIED GLEEKDYEGW KLLTERLGSK IMLVGDDLYT TNTRLIKQGI EEKWANALLL KVNQIGTITE AMNAARMIFN VGQKVIVSHR SGETATTLIS DLVVGIGATH IKTGATARGE RVSKYNRLLQ IEEYLEQHGL LA
NT Sequence
ATGCTTTATT TGGTGCTTAT GCTTGTTGAG AATGGGTTTG GCCAAAATTG TTTTCTTCAC AGCCAAAAAA TTTGCTCCAA CATGACCGAT AAACAACCAG TCAGTGAATA TCTTCAAAAT CACAATCTTC AAAAGTTGGT TGAAGATGCT CTCAATGAAT GCTACAATGC CAACGCCAGT GATCCCGTTG GATTCCTTGG TCACTTCTTT TTGAACCGTG GAAAGAAGGG TGCTGTCAAT CGTGTCGACA AGCTTGTTGG TAGAGAAATT TTGGATTCAA GAGGTAATCC AACGGTTGAA GTTGACGTTT ATGCTAATGG ACAAAAGAGA CCAGTAGCCA CTGCTTCTGC ACCATCCGGA GCAAGTACAG GAAGCAATGA AGCTCACGAA CTTCGTGATG GTGATAAATC AAGATACCTC GGAAAAGGTG TTTTGAAAGC TGTGAAGAAT GTGAATGATG TGTTGGGCAA GGCAGTGGAA GGAAAGTCAT TGGAAAACTT GACAGAACTC GATCAAGCTT TGATCGATGC TGATGGTGAT GAGTTGAAGA GCAATTTGGG AGGTAATGCG ATCACTGCAT GCAGTTTTGC ACTTGCCACT GCTGGTGCTG CTGTCAGAAA TGAGGAATTG TTCTTGTACT TGGCAAGAGC ATTCCATGGA GCTGATAAGT TTGAAAACTT AAAGTTCCGT TTGCCTACTC CTATGGTCAA CATTTTGAAT GGAGGTAAAC ACGCAGGTGG TCGTCTCCAA ATTCAAGAAT TTATGATTTT ACCAAAGGAA AATCAACCAT TCCGTGAGAA GGTGAGATGT GTCGCTGAAG TTTATCAACA TTTGGGTAAG ATTCTTGCTG AGAGAGCTGG TCCAAGTGCA AAGAACGTTG GTGATGAAGG AGGATTTGCA CCAAACTTGG AAACTGCTGA TGAAGCATTG AATTATATCG AGGAAGCAAT TGGCAAGGCA GGCTACAAGG TTGGCGAAGA TGTGTTTTTG GCTTTGGATG CTGCTTCAAG TGAGTTCTAC AACTCGGACA CAAAGAAGTA TGAAATTACT CAACAAAAAG AGTTCTTGAC CTCCGAAGAA ATGGTTGAAT ATTATGTTCA ATTGGTCAAT AGACACCCTG CTATTATCTC CATTGAAGAT GGATTGGAAG AAAAGGATTA TGAAGGATGG AAACTTTTGA CTGAAAGATT AGGATCAAAG ATCATGTTGG TTGGTGATGA TTTGTACACA ACAAATACCA GATTAATCAA GCAAGGAATT GAGGAAAAGT GGGCAAATGC TTTGTTGTTG AAGGTTAACC AAATTGGTAC CATCACCGAA GCAATGAATG CTGCCAGAAT GATTTTCAAT GTTGGACAAA AGGTTATTGT TTCGCATAGA TCCGGTGAGA CTGCAACCAC TTTAATCTCT GACTTGGTGG TTGGTATTGG AGCCACACAC ATTAAGACTG GTGCTACTGC TAGAGGAGAA CGTGTATCCA AGTACAACCG ATTGTTGCAA ATCGAAGAAT ATCTTGAACA ACACGGTTTG CTTGCA
Details for NafoA.00379.a.B4.GE44253
| HARVESTED ON: | 4/1/2021 |
| SEQUENCED ON: | 4/13/2021 |
| EXPECTED MW: | 50kDa |
| OBSERVED MW: | 50kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHPV GFLGHFFLNR GKKGAVNRVD KLVGREILDS RGNPTVEVDV YANGQKRPVA TASAPSGAST GSNEAHELRD GDKSRYLGKG VLKAVKNVND VLGKAVEGKS LENLTELDQA LIDADGDELK SNLGGNAITA CSFALATAGA AVRNEELFLY LARAFHGADK FENLKFRLPT PMVNILNGGK HAGGRLQIQE FMILPKENQP FREKVRCVAE VYQHLGKILA ERAGPSAKNV GDEGGFAPNL ETADEALNYI EEAIGKAGYK VGEDVFLALD AASSEFYNSD TKKYEITQQK EFLTSEEMVE YYVQLVNRHP AIISIEDGLE EKDYEGWKLL TERLGSKIML VGDDLYTTNT RLIKQGIEEK WANALLLKVN QIGTITEAMN AARMIFNVGQ KVIVSHRSGE TATTLISDLV VGIGATHIKT GATARGERVS KYNRLLQIEE YLEQHGLLA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catcccgttg gattccttgg tcacttcttt ttgaaccgtg gaaagaaggg tgctgtcaat cgtgtcgaca agcttgttgg tagagaaatt ttggattcaa gaggtaatcc aacggttgaa gttgacgttt atgctaatgg acaaaagaga ccagtagcca ctgcttctgc accatccgga gcaagtacag gaagcaatga agctcacgaa cttcgtgatg gtgataaatc aagatacctc ggaaaaggtg ttttgaaagc tgtgaagaat gtgaatgatg tgttgggcaa ggcagtggaa ggaaagtcat tggaaaactt gacagaactc gatcaagctt tgatcgatgc tgatggtgat gagttgaaga gcaatttggg aggtaatgcg atcactgcat gcagttttgc acttgccact gctggtgctg ctgtcagaaa tgaggaattg ttcttgtact tggcaagagc attccatgga gctgataagt ttgaaaactt aaagttccgt ttgcctactc ctatggtcaa cattttgaat ggaggtaaac acgcaggtgg tcgtctccaa attcaagaat ttatgatttt accaaaggaa aatcaaccat tccgtgagaa ggtgagatgt gtcgctgaag tttatcaaca tttgggtaag attcttgctg agagagctgg tccaagtgca aagaacgttg gtgatgaagg aggatttgca ccaaacttgg aaactgctga tgaagcattg aattatatcg aggaagcaat tggcaaggca ggctacaagg ttggcgaaga tgtgtttttg gctttggatg ctgcttcaag tgagttctac aactcggaca caaagaagta tgaaattact caacaaaaag agttcttgac ctccgaagaa atggttgaat attatgttca attggtcaat agacaccctg ctattatctc cattgaagat ggattggaag aaaaggatta tgaaggatgg aaacttttga ctgaaagatt aggatcaaag atcatgttgg ttggtgatga tttgtacaca acaaatacca gattaatcaa gcaaggaatt gaggaaaagt gggcaaatgc tttgttgttg aaggttaacc aaattggtac catcaccgaa gcaatgaatg ctgccagaat gattttcaat gttggacaaa aggttattgt ttcgcataga tccggtgaga ctgcaaccac tttaatctct gacttggtgg ttggtattgg agccacacac attaagactg gtgctactgc tagaggagaa cgtgtatcca agtacaaccg attgttgcaa atcgaagaat atcttgaaca acacggtttg cttgcatgag taagatagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc agtgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc gagatctcga tcccgcgaaa t
Details for NafoA.00379.a.B5.GE44254
| HARVESTED ON: | 4/1/2021 |
| SEQUENCED ON: | 4/13/2021 |
| EXPECTED MW: | 52kDa |
| OBSERVED MW: | 52kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Good (10-50) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 95 |
| PERCENT COVERAGE: | 63 |
Validated AA Sequence
MAHHHHHHMQ KLVEDALNEC YNANASDPVG FLGHFFLNRG KKGAVNRVDK LVGREILDSR GNPTVEVDVY ANGQKRPVAT ASAPSGASTG SNEAHELRDG DKSRYLGKGV LKAVKNVNDV LGKAVEGKSL ENLTELDQAL IDADGDELKS NLGGNAITAC SFALATAGAA VRNEELFLYL ARAFHGADKF ENLKFRLPTP MVNILNGGKH AGGRLQIQEF MILPKENQPF REKVRCVAEV YQHLGKILAE RAGPSAKNVG DEGGXAPNLE TADEALNYIE EAIGQGRLXX WRXVFWLXML LQ
Validated NT Sequence
acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta nnaaggagat ataccatggc tcaccaccac caccaccata tgcaaaagtt ggttgaagat gctctcaatg aatgctacaa tgccaacgcc agtgatcccg ttggattcct tggtcacttc tttttgaacc gtggaaagaa gggtgctgtc aatcgtgtcg acaagcttgt cggtagagaa attttggatt caagaggtaa tccaacggtt gaagttgacg tttatgctaa tggacaaaag agaccagtag ccactgcttc tgcaccatcc ggagcaagta caggaagcaa tgaagctcac gaacttcgtg atggtgataa atcaagatac ctcggaaaag gtgttttgaa agctgtgaag aatgtgaatg atgtgttggg caaggcagtg gaaggaaagt cattggaaaa cttgacagaa ctcgatcaag ctttgatcga tgctgatggt gatgagttga agagcaattt gggaggtaat gcgatcactg catgcagttt tgcacttgcc actgctggtg ctgctgtcag aaatgaggaa ttgttcttgt acttggcaag agcattccat ggagctgata agtttgaaaa cttaaagttc cgtttgccta ctcctatggt caacattttg aatggaggta aacacgcagg tggtcgtctc caaattcaag aatttatgat tttaccaaag gaaaatcaac cattccgtga gaaggtgaga tgtgtcgctg aagtttatca acatttgggt aagattcttg ctgagagagc tggtccaagt gcnaagaacg ttggtgatga aggaggantt gcaccnaact tggaaactgc tgatgaagca ttgaattata tcgaggaagc aattgggcaa ggcaggctan naanntggcg anatgtgttt tggctttnga tgctgcttca gtgagttcta nactcggacn ctaanaagta tgaaattant cnncaaaaga tnntgacctn cgaagaannn nnaanntatn tcantngnca annnnnannc cctgctnnnt annnncattg annangggat tgnnnnnnaa a
Expected Protein Sequence
MAHHHHHHQK LVEDALNECY NANASDPVGF LGHFFLNRGK KGAVNRVDKL VGREILDSRG NPTVEVDVYA NGQKRPVATA SAPSGASTGS NEAHELRDGD KSRYLGKGVL KAVKNVNDVL GKAVEGKSLE NLTELDQALI DADGDELKSN LGGNAITACS FALATAGAAV RNEELFLYLA RAFHGADKFE NLKFRLPTPM VNILNGGKHA GGRLQIQEFM ILPKENQPFR EKVRCVAEVY QHLGKILAER AGPSAKNVGD EGGFAPNLET ADEALNYIEE AIGKAGYKVG EDVFLALDAA SSEFYNSDTK KYEITQQKEF LTSEEMVEYY VQLVNRHPAI ISIEDGLEEK DYEGWKLLTE RLGSKIMLVG DDLYTTNTRL IKQGIEEKWA NALLLKVNQI GTITEAMNAA RMIFNVGQKV IVSHRSGETA TTLISDLVVG IGATHIKTGA TARGERVSKY NRLLQIEEYL EQHGLLA
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catcaaaagt tggttgaaga tgctctcaat gaatgctaca atgccaacgc cagtgatccc gttggattcc ttggtcactt ctttttgaac cgtggaaaga agggtgctgt caatcgtgtc gacaagcttg ttggtagaga aattttggat tcaagaggta atccaacggt tgaagttgac gtttatgcta atggacaaaa gagaccagta gccactgctt ctgcaccatc cggagcaagt acaggaagca atgaagctca cgaacttcgt gatggtgata aatcaagata cctcggaaaa ggtgttttga aagctgtgaa gaatgtgaat gatgtgttgg gcaaggcagt ggaaggaaag tcattggaaa acttgacaga actcgatcaa gctttgatcg atgctgatgg tgatgagttg aagagcaatt tgggaggtaa tgcgatcact gcatgcagtt ttgcacttgc cactgctggt gctgctgtca gaaatgagga attgttcttg tacttggcaa gagcattcca tggagctgat aagtttgaaa acttaaagtt ccgtttgcct actcctatgg tcaacatttt gaatggaggt aaacacgcag gtggtcgtct ccaaattcaa gaatttatga ttttaccaaa ggaaaatcaa ccattccgtg agaaggtgag atgtgtcgct gaagtttatc aacatttggg taagattctt gctgagagag ctggtccaag tgcaaagaac gttggtgatg aaggaggatt tgcaccaaac ttggaaactg ctgatgaagc attgaattat atcgaggaag caattggcaa ggcaggctac aaggttggcg aagatgtgtt tttggctttg gatgctgctt caagtgagtt ctacaactcg gacacaaaga agtatgaaat tactcaacaa aaagagttct tgacctccga agaaatggtt gaatattatg ttcaattggt caatagacac cctgctatta tctccattga agatggattg gaagaaaagg attatgaagg atggaaactt ttgactgaaa gattaggatc aaagatcatg ttggttggtg atgatttgta cacaacaaat accagattaa tcaagcaagg aattgaggaa aagtgggcaa atgctttgtt gttgaaggtt aaccaaattg gtaccatcac cgaagcaatg aatgctgcca gaatgatttt caatgttgga caaaaggtta ttgtttcgca tagatccggt gagactgcaa ccactttaat ctctgacttg gtggttggta ttggagccac acacattaag actggtgcta ctgctagagg agaacgtgta tccaagtaca accgattgtt gcaaatcgaa gaatatcttg aacaacacgg tttgcttgca tgagtaagat aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg tggccaggac ccaacgctgc ccgagatgcg ccgcgtgcgg ctgctggaga tggcggacgc gatggatatg ttctgccaag ggttggtttg cgcattcaca gttctccgca agaattgatt ggctccaatt cttggagtgg tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgcctacaa tccatgccaa cccgttccat gtgctcgccg aggcggcata aatcgccgtg acgatcagcg gtccagtgat cgaagttagg ctggtaagag ccgcgagcga tccttgaagc tgtccctgat ggtcgtcatc tacctgcctg gacagcatgg cctgcaacgc gggcatcccg atgccgccgg aagcgagaag aatcataatg gggaaggcca tccagcctcg cgtcgcgaac gccagcaaga cgtagcccag cgcgtcggcc gccatgccgg cgataatggc ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg gttgaaggct ctcaagggca tcggtcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatcgagatc tcgatcccgc gaaat
Details for NafoA.00379.a.B4.PW38978
| PURIFICATION DATe: | 4/19/2021 |
| CONCENTRATION: | 26mg/ml |
| OBSERVED MW: | 50kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| VIAL COUNT (approx.): | 8 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHPV GFLGHFFLNR GKKGAVNRVD KLVGREILDS RGNPTVEVDV YANGQKRPVA TASAPSGAST GSNEAHELRD GDKSRYLGKG VLKAVKNVND VLGKAVEGKS LENLTELDQA LIDADGDELK SNLGGNAITA CSFALATAGA AVRNEELFLY LARAFHGADK FENLKFRLPT PMVNILNGGK HAGGRLQIQE FMILPKENQP FREKVRCVAE VYQHLGKILA ERAGPSAKNV GDEGGFAPNL ETADEALNYI EEAIGKAGYK VGEDVFLALD AASSEFYNSD TKKYEITQQK EFLTSEEMVE YYVQLVNRHP AIISIEDGLE EKDYEGWKLL TERLGSKIML VGDDLYTTNT RLIKQGIEEK WANALLLKVN QIGTITEAMN AARMIFNVGQ KVIVSHRSGE TATTLISDLV VGIGATHIKT GATARGERVS KYNRLLQIEE YLEQHGLLA
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATCCCGTTG GATTCCTTGG TCACTTCTTT TTGAACCGTG GAAAGAAGGG TGCTGTCAAT CGTGTCGACA AGCTTGTTGG TAGAGAAATT TTGGATTCAA GAGGTAATCC AACGGTTGAA GTTGACGTTT ATGCTAATGG ACAAAAGAGA CCAGTAGCCA CTGCTTCTGC ACCATCCGGA GCAAGTACAG GAAGCAATGA AGCTCACGAA CTTCGTGATG GTGATAAATC AAGATACCTC GGAAAAGGTG TTTTGAAAGC TGTGAAGAAT GTGAATGATG TGTTGGGCAA GGCAGTGGAA GGAAAGTCAT TGGAAAACTT GACAGAACTC GATCAAGCTT TGATCGATGC TGATGGTGAT GAGTTGAAGA GCAATTTGGG AGGTAATGCG ATCACTGCAT GCAGTTTTGC ACTTGCCACT GCTGGTGCTG CTGTCAGAAA TGAGGAATTG TTCTTGTACT TGGCAAGAGC ATTCCATGGA GCTGATAAGT TTGAAAACTT AAAGTTCCGT TTGCCTACTC CTATGGTCAA CATTTTGAAT GGAGGTAAAC ACGCAGGTGG TCGTCTCCAA ATTCAAGAAT TTATGATTTT ACCAAAGGAA AATCAACCAT TCCGTGAGAA GGTGAGATGT GTCGCTGAAG TTTATCAACA TTTGGGTAAG ATTCTTGCTG AGAGAGCTGG TCCAAGTGCA AAGAACGTTG GTGATGAAGG AGGATTTGCA CCAAACTTGG AAACTGCTGA TGAAGCATTG AATTATATCG AGGAAGCAAT TGGCAAGGCA GGCTACAAGG TTGGCGAAGA TGTGTTTTTG GCTTTGGATG CTGCTTCAAG TGAGTTCTAC AACTCGGACA CAAAGAAGTA TGAAATTACT CAACAAAAAG AGTTCTTGAC CTCCGAAGAA ATGGTTGAAT ATTATGTTCA ATTGGTCAAT AGACACCCTG CTATTATCTC CATTGAAGAT GGATTGGAAG AAAAGGATTA TGAAGGATGG AAACTTTTGA CTGAAAGATT AGGATCAAAG ATCATGTTGG TTGGTGATGA TTTGTACACA ACAAATACCA GATTAATCAA GCAAGGAATT GAGGAAAAGT GGGCAAATGC TTTGTTGTTG AAGGTTAACC AAATTGGTAC CATCACCGAA GCAATGAATG CTGCCAGAAT GATTTTCAAT GTTGGACAAA AGGTTATTGT TTCGCATAGA TCCGGTGAGA CTGCAACCAC TTTAATCTCT GACTTGGTGG TTGGTATTGG AGCCACACAC ATTAAGACTG GTGCTACTGC TAGAGGAGAA CGTGTATCCA AGTACAACCG ATTGTTGCAA ATCGAAGAAT ATCTTGAACA ACACGGTTTG CTTGCATGAG TAAGATAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AATTCTTGAA GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTGTTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGCAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCTGA TGCGGTATTT TCTCCTTACG CATCTGTGCG GTATTTCACA CCGCATATAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT ACACTCCGCT ATCGCTACGT GACTGGGTCA TGGCTGCGCC CCGACACCCG CCAACACCCG CTGACGCGCC CTGACGGGCT TGTCTGCTCC CGGCATCCGC TTACAGACAA GCTGTGACCG TCTCCGGGAG CTGCATGTGT CAGAGGTTTT CACCGTCATC ACCGAAACGC GCGAGGCAGC TGCGGTAAAG CTCATCAGCG TGGTCGTGAA GCGATTCACA GATGTCTGCC TGTTCATCCG CGTCCAGCTC GTTGAGTTTC TCCAGAAGCG TTAATGTCTG GCTTCTGATA AAGCGGGCCA TGTTAAGGGC GGTTTTTTCC TGTTTGGTCA CTGATGCCTC CGTGTAAGGG GGATTTCTGT TCATGGGGGT AATGATACCG ATGAAACGAG AGAGGATGCT CACGATACGG GTTACTGATG ATGAACATGC CCGGTTACTG GAACGTTGTG AGGGTAAACA ACTGGCGGTA TGGATGCGGC GGGACCAGAG AAAAATCACT CAGGGTCAAT GCCAGCGCTT CGTTAATACA GATGTAGGTG TTCCACAGGG TAGCCAGCAG CATCCTGCGA TGCAGATCCG GAACATAATG GTGCAGGGCG CTGACTTCCG CGTTTCCAGA CTTTACGAAA CACGGAAACC GAAGACCATT CATGTTGTTG CTCAGGTCGC AGACGTTTTG CAGCAGCAGT CGCTTCACGT TCGCTCGCGT ATCGGTGATT CATTCTGCTA ACCAGTAAGG CAACCCCGCC AGCCTAGCCG GGTCCTCAAC GACAGGAGCA CGATCATGCG CACCCGTGGC CAGGACCCAA CGCTGCCCGA GATGCGCCGC GTGCGGCTGC TGGAGATGGC GGACGCGATG GATATGTTCT GCCAAGGGTT GGTTTGCGCA TTCACAGTTC TCCGCAAGAA TTGATTGGCT CCAATTCTTG GAGTGGTGAA TCCGTTAGCG AGGTGCCGCC GGCTTCCATT CAGGTCGAGG TGGCCCGGCT CCATGCACCG CGACGCAACG CGGGGAGGCA GACAAGGTAT AGGGCGGCGC CTACAATCCA TGCCAACCCG TTCCATGTGC TCGCCGAGGC GGCATAAATC GCCGTGACGA TCAGCGGTCC AGTGATCGAA GTTAGGCTGG TAAGAGCCGC GAGCGATCCT TGAAGCTGTC CCTGATGGTC GTCATCTACC TGCCTGGACA GCATGGCCTG CAACGCGGGC ATCCCGATGC CGCCGGAAGC GAGAAGAATC ATAATGGGGA AGGCCATCCA GCCTCGCGTC GCGAACGCCA GCAAGACGTA GCCCAGCGCG TCGGCCGCCA TGCCGGCGAT AATGGCCTGC TTCTCGCCGA AACGTTTGGT GGCGGGACCA GTGACGAAGG CTTGAGCGAG GGCGTGCAAG ATTCCGAATA CCGCAAGCGA CAGGCCGATC ATCGTCGCGC TCCAGCGAAA GCGGTCCTCG CCGAAAATGA CCCAGAGCGC TGCCGGCACC TGTCCTACGA GTTGCATGAT AAAGAAGACA GTCATAAGTG CGGCGACGAT AGTCATGCCC CGCGCCCACC GGAAGGAGCT GACTGGGTTG AAGGCTCTCA AGGGCATCGG TCGACGCTCT CCCTTATGCG ACTCCTGCAT TAGGAAGCAG CCCAGTAGTA GGTTGAGGCC GTTGAGCACC GCCGCCGCAA GGAATGGTGC ATGCAAGGAG ATGGCGCCCA ACAGTCCCCC GGCCACGGGG CCTGCCACCA TACCCACGCC GAAACAAGCG CTCATGAGCC CGAAGTGGCG AGCCCGATCT TCCCCATCGG TGATGTCGGC GATATAGGCG CCAGCAACCG CACCTGTGGC GCCGGTGATG CCGGCCACGA TGCGTCCGGC GTAGAGGATC GAGATCTCGA TCCCGCGAAA T
Details for NafoA.00379.a.B5.PW38980
| PURIFICATION DATe: | 4/21/2021 |
| CONCENTRATION: | 36mg/ml |
| OBSERVED MW: | 52kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| VIAL COUNT (approx.): | 6 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 95 |
| PERCENT COVERAGE: | 63 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMQ KLVEDALNEC YNANASDPVG FLGHFFLNRG KKGAVNRVDK LVGREILDSR GNPTVEVDVY ANGQKRPVAT ASAPSGASTG SNEAHELRDG DKSRYLGKGV LKAVKNVNDV LGKAVEGKSL ENLTELDQAL IDADGDELKS NLGGNAITAC SFALATAGAA VRNEELFLYL ARAFHGADKF ENLKFRLPTP MVNILNGGKH AGGRLQIQEF MILPKENQPF REKVRCVAEV YQHLGKILAE RAGPSAKNVG DEGGXAPNLE TADEALNYIE EAIGQGRLXX WRXVFWLXML LQ
Validated NT Sequence
acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta nnaaggagat ataccatggc tcaccaccac caccaccata tgcaaaagtt ggttgaagat gctctcaatg aatgctacaa tgccaacgcc agtgatcccg ttggattcct tggtcacttc tttttgaacc gtggaaagaa gggtgctgtc aatcgtgtcg acaagcttgt cggtagagaa attttggatt caagaggtaa tccaacggtt gaagttgacg tttatgctaa tggacaaaag agaccagtag ccactgcttc tgcaccatcc ggagcaagta caggaagcaa tgaagctcac gaacttcgtg atggtgataa atcaagatac ctcggaaaag gtgttttgaa agctgtgaag aatgtgaatg atgtgttggg caaggcagtg gaaggaaagt cattggaaaa cttgacagaa ctcgatcaag ctttgatcga tgctgatggt gatgagttga agagcaattt gggaggtaat gcgatcactg catgcagttt tgcacttgcc actgctggtg ctgctgtcag aaatgaggaa ttgttcttgt acttggcaag agcattccat ggagctgata agtttgaaaa cttaaagttc cgtttgccta ctcctatggt caacattttg aatggaggta aacacgcagg tggtcgtctc caaattcaag aatttatgat tttaccaaag gaaaatcaac cattccgtga gaaggtgaga tgtgtcgctg aagtttatca acatttgggt aagattcttg ctgagagagc tggtccaagt gcnaagaacg ttggtgatga aggaggantt gcaccnaact tggaaactgc tgatgaagca ttgaattata tcgaggaagc aattgggcaa ggcaggctan naanntggcg anatgtgttt tggctttnga tgctgcttca gtgagttcta nactcggacn ctaanaagta tgaaattant cnncaaaaga tnntgacctn cgaagaannn nnaanntatn tcantngnca annnnnannc cctgctnnnt annnncattg annangggat tgnnnnnnaa a
Expressed Protein Sequence
MAHHHHHHQK LVEDALNECY NANASDPVGF LGHFFLNRGK KGAVNRVDKL VGREILDSRG NPTVEVDVYA NGQKRPVATA SAPSGASTGS NEAHELRDGD KSRYLGKGVL KAVKNVNDVL GKAVEGKSLE NLTELDQALI DADGDELKSN LGGNAITACS FALATAGAAV RNEELFLYLA RAFHGADKFE NLKFRLPTPM VNILNGGKHA GGRLQIQEFM ILPKENQPFR EKVRCVAEVY QHLGKILAER AGPSAKNVGD EGGFAPNLET ADEALNYIEE AIGKAGYKVG EDVFLALDAA SSEFYNSDTK KYEITQQKEF LTSEEMVEYY VQLVNRHPAI ISIEDGLEEK DYEGWKLLTE RLGSKIMLVG DDLYTTNTRL IKQGIEEKWA NALLLKVNQI GTITEAMNAA RMIFNVGQKV IVSHRSGETA TTLISDLVVG IGATHIKTGA TARGERVSKY NRLLQIEEYL EQHGLLA
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATCAAAAGT TGGTTGAAGA TGCTCTCAAT GAATGCTACA ATGCCAACGC CAGTGATCCC GTTGGATTCC TTGGTCACTT CTTTTTGAAC CGTGGAAAGA AGGGTGCTGT CAATCGTGTC GACAAGCTTG TTGGTAGAGA AATTTTGGAT TCAAGAGGTA ATCCAACGGT TGAAGTTGAC GTTTATGCTA ATGGACAAAA GAGACCAGTA GCCACTGCTT CTGCACCATC CGGAGCAAGT ACAGGAAGCA ATGAAGCTCA CGAACTTCGT GATGGTGATA AATCAAGATA CCTCGGAAAA GGTGTTTTGA AAGCTGTGAA GAATGTGAAT GATGTGTTGG GCAAGGCAGT GGAAGGAAAG TCATTGGAAA ACTTGACAGA ACTCGATCAA GCTTTGATCG ATGCTGATGG TGATGAGTTG AAGAGCAATT TGGGAGGTAA TGCGATCACT GCATGCAGTT TTGCACTTGC CACTGCTGGT GCTGCTGTCA GAAATGAGGA ATTGTTCTTG TACTTGGCAA GAGCATTCCA TGGAGCTGAT AAGTTTGAAA ACTTAAAGTT CCGTTTGCCT ACTCCTATGG TCAACATTTT GAATGGAGGT AAACACGCAG GTGGTCGTCT CCAAATTCAA GAATTTATGA TTTTACCAAA GGAAAATCAA CCATTCCGTG AGAAGGTGAG ATGTGTCGCT GAAGTTTATC AACATTTGGG TAAGATTCTT GCTGAGAGAG CTGGTCCAAG TGCAAAGAAC GTTGGTGATG AAGGAGGATT TGCACCAAAC TTGGAAACTG CTGATGAAGC ATTGAATTAT ATCGAGGAAG CAATTGGCAA GGCAGGCTAC AAGGTTGGCG AAGATGTGTT TTTGGCTTTG GATGCTGCTT CAAGTGAGTT CTACAACTCG GACACAAAGA AGTATGAAAT TACTCAACAA AAAGAGTTCT TGACCTCCGA AGAAATGGTT GAATATTATG TTCAATTGGT CAATAGACAC CCTGCTATTA TCTCCATTGA AGATGGATTG GAAGAAAAGG ATTATGAAGG ATGGAAACTT TTGACTGAAA GATTAGGATC AAAGATCATG TTGGTTGGTG ATGATTTGTA CACAACAAAT ACCAGATTAA TCAAGCAAGG AATTGAGGAA AAGTGGGCAA ATGCTTTGTT GTTGAAGGTT AACCAAATTG GTACCATCAC CGAAGCAATG AATGCTGCCA GAATGATTTT CAATGTTGGA CAAAAGGTTA TTGTTTCGCA TAGATCCGGT GAGACTGCAA CCACTTTAAT CTCTGACTTG GTGGTTGGTA TTGGAGCCAC ACACATTAAG ACTGGTGCTA CTGCTAGAGG AGAACGTGTA TCCAAGTACA ACCGATTGTT GCAAATCGAA GAATATCTTG AACAACACGG TTTGCTTGCA TGAGTAAGAT AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAATTCT TGAAGACGAA AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTGTT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGCA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CTGATGCGGT ATTTTCTCCT TACGCATCTG TGCGGTATTT CACACCGCAT ATATGGTGCA CTCTCAGTAC AATCTGCTCT GATGCCGCAT AGTTAAGCCA GTATACACTC CGCTATCGCT ACGTGACTGG GTCATGGCTG CGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCGTCTCCG GGAGCTGCAT GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGAGG CAGCTGCGGT AAAGCTCATC AGCGTGGTCG TGAAGCGATT CACAGATGTC TGCCTGTTCA TCCGCGTCCA GCTCGTTGAG TTTCTCCAGA AGCGTTAATG TCTGGCTTCT GATAAAGCGG GCCATGTTAA GGGCGGTTTT TTCCTGTTTG GTCACTGATG CCTCCGTGTA AGGGGGATTT CTGTTCATGG GGGTAATGAT ACCGATGAAA CGAGAGAGGA TGCTCACGAT ACGGGTTACT GATGATGAAC ATGCCCGGTT ACTGGAACGT TGTGAGGGTA AACAACTGGC GGTATGGATG CGGCGGGACC AGAGAAAAAT CACTCAGGGT CAATGCCAGC GCTTCGTTAA TACAGATGTA GGTGTTCCAC AGGGTAGCCA GCAGCATCCT GCGATGCAGA TCCGGAACAT AATGGTGCAG GGCGCTGACT TCCGCGTTTC CAGACTTTAC GAAACACGGA AACCGAAGAC CATTCATGTT GTTGCTCAGG TCGCAGACGT TTTGCAGCAG CAGTCGCTTC ACGTTCGCTC GCGTATCGGT GATTCATTCT GCTAACCAGT AAGGCAACCC CGCCAGCCTA GCCGGGTCCT CAACGACAGG AGCACGATCA TGCGCACCCG TGGCCAGGAC CCAACGCTGC CCGAGATGCG CCGCGTGCGG CTGCTGGAGA TGGCGGACGC GATGGATATG TTCTGCCAAG GGTTGGTTTG CGCATTCACA GTTCTCCGCA AGAATTGATT GGCTCCAATT CTTGGAGTGG TGAATCCGTT AGCGAGGTGC CGCCGGCTTC CATTCAGGTC GAGGTGGCCC GGCTCCATGC ACCGCGACGC AACGCGGGGA GGCAGACAAG GTATAGGGCG GCGCCTACAA TCCATGCCAA CCCGTTCCAT GTGCTCGCCG AGGCGGCATA AATCGCCGTG ACGATCAGCG GTCCAGTGAT CGAAGTTAGG CTGGTAAGAG CCGCGAGCGA TCCTTGAAGC TGTCCCTGAT GGTCGTCATC TACCTGCCTG GACAGCATGG CCTGCAACGC GGGCATCCCG ATGCCGCCGG AAGCGAGAAG AATCATAATG GGGAAGGCCA TCCAGCCTCG CGTCGCGAAC GCCAGCAAGA CGTAGCCCAG CGCGTCGGCC GCCATGCCGG CGATAATGGC CTGCTTCTCG CCGAAACGTT TGGTGGCGGG ACCAGTGACG AAGGCTTGAG CGAGGGCGTG CAAGATTCCG AATACCGCAA GCGACAGGCC GATCATCGTC GCGCTCCAGC GAAAGCGGTC CTCGCCGAAA ATGACCCAGA GCGCTGCCGG CACCTGTCCT ACGAGTTGCA TGATAAAGAA GACAGTCATA AGTGCGGCGA CGATAGTCAT GCCCCGCGCC CACCGGAAGG AGCTGACTGG GTTGAAGGCT CTCAAGGGCA TCGGTCGACG CTCTCCCTTA TGCGACTCCT GCATTAGGAA GCAGCCCAGT AGTAGGTTGA GGCCGTTGAG CACCGCCGCC GCAAGGAATG GTGCATGCAA GGAGATGGCG CCCAACAGTC CCCCGGCCAC GGGGCCTGCC ACCATACCCA CGCCGAAACA AGCGCTCATG AGCCCGAAGT GGCGAGCCCG ATCTTCCCCA TCGGTGATGT CGGCGATATA GGCGCCAGCA ACCGCACCTG TGGCGCCGGT GATGCCGGCC ACGATGCGTC CGGCGTAGAG GATCGAGATC TCGATCCCGC GAAAT
Details for NafoA.00379.a.B5.PW39044
| PURIFICATION DATe: | 6/11/2021 |
| CONCENTRATION: | 54mg/ml |
| OBSERVED MW: | 52kDa |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| VIAL COUNT (approx.): | 4 |
| VIAL VOLUME: | 50µl |
| PERCENT IDENTITY: | 95 |
| PERCENT COVERAGE: | 63 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMQ KLVEDALNEC YNANASDPVG FLGHFFLNRG KKGAVNRVDK LVGREILDSR GNPTVEVDVY ANGQKRPVAT ASAPSGASTG SNEAHELRDG DKSRYLGKGV LKAVKNVNDV LGKAVEGKSL ENLTELDQAL IDADGDELKS NLGGNAITAC SFALATAGAA VRNEELFLYL ARAFHGADKF ENLKFRLPTP MVNILNGGKH AGGRLQIQEF MILPKENQPF REKVRCVAEV YQHLGKILAE RAGPSAKNVG DEGGXAPNLE TADEALNYIE EAIGQGRLXX WRXVFWLXML LQ
Validated NT Sequence
acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta nnaaggagat ataccatggc tcaccaccac caccaccata tgcaaaagtt ggttgaagat gctctcaatg aatgctacaa tgccaacgcc agtgatcccg ttggattcct tggtcacttc tttttgaacc gtggaaagaa gggtgctgtc aatcgtgtcg acaagcttgt cggtagagaa attttggatt caagaggtaa tccaacggtt gaagttgacg tttatgctaa tggacaaaag agaccagtag ccactgcttc tgcaccatcc ggagcaagta caggaagcaa tgaagctcac gaacttcgtg atggtgataa atcaagatac ctcggaaaag gtgttttgaa agctgtgaag aatgtgaatg atgtgttggg caaggcagtg gaaggaaagt cattggaaaa cttgacagaa ctcgatcaag ctttgatcga tgctgatggt gatgagttga agagcaattt gggaggtaat gcgatcactg catgcagttt tgcacttgcc actgctggtg ctgctgtcag aaatgaggaa ttgttcttgt acttggcaag agcattccat ggagctgata agtttgaaaa cttaaagttc cgtttgccta ctcctatggt caacattttg aatggaggta aacacgcagg tggtcgtctc caaattcaag aatttatgat tttaccaaag gaaaatcaac cattccgtga gaaggtgaga tgtgtcgctg aagtttatca acatttgggt aagattcttg ctgagagagc tggtccaagt gcnaagaacg ttggtgatga aggaggantt gcaccnaact tggaaactgc tgatgaagca ttgaattata tcgaggaagc aattgggcaa ggcaggctan naanntggcg anatgtgttt tggctttnga tgctgcttca gtgagttcta nactcggacn ctaanaagta tgaaattant cnncaaaaga tnntgacctn cgaagaannn nnaanntatn tcantngnca annnnnannc cctgctnnnt annnncattg annangggat tgnnnnnnaa a
Expressed Protein Sequence
MAHHHHHHQK LVEDALNECY NANASDPVGF LGHFFLNRGK KGAVNRVDKL VGREILDSRG NPTVEVDVYA NGQKRPVATA SAPSGASTGS NEAHELRDGD KSRYLGKGVL KAVKNVNDVL GKAVEGKSLE NLTELDQALI DADGDELKSN LGGNAITACS FALATAGAAV RNEELFLYLA RAFHGADKFE NLKFRLPTPM VNILNGGKHA GGRLQIQEFM ILPKENQPFR EKVRCVAEVY QHLGKILAER AGPSAKNVGD EGGFAPNLET ADEALNYIEE AIGKAGYKVG EDVFLALDAA SSEFYNSDTK KYEITQQKEF LTSEEMVEYY VQLVNRHPAI ISIEDGLEEK DYEGWKLLTE RLGSKIMLVG DDLYTTNTRL IKQGIEEKWA NALLLKVNQI GTITEAMNAA RMIFNVGQKV IVSHRSGETA TTLISDLVVG IGATHIKTGA TARGERVSKY NRLLQIEEYL EQHGLLA
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATCAAAAGT TGGTTGAAGA TGCTCTCAAT GAATGCTACA ATGCCAACGC CAGTGATCCC GTTGGATTCC TTGGTCACTT CTTTTTGAAC CGTGGAAAGA AGGGTGCTGT CAATCGTGTC GACAAGCTTG TTGGTAGAGA AATTTTGGAT TCAAGAGGTA ATCCAACGGT TGAAGTTGAC GTTTATGCTA ATGGACAAAA GAGACCAGTA GCCACTGCTT CTGCACCATC CGGAGCAAGT ACAGGAAGCA ATGAAGCTCA CGAACTTCGT GATGGTGATA AATCAAGATA CCTCGGAAAA GGTGTTTTGA AAGCTGTGAA GAATGTGAAT GATGTGTTGG GCAAGGCAGT GGAAGGAAAG TCATTGGAAA ACTTGACAGA ACTCGATCAA GCTTTGATCG ATGCTGATGG TGATGAGTTG AAGAGCAATT TGGGAGGTAA TGCGATCACT GCATGCAGTT TTGCACTTGC CACTGCTGGT GCTGCTGTCA GAAATGAGGA ATTGTTCTTG TACTTGGCAA GAGCATTCCA TGGAGCTGAT AAGTTTGAAA ACTTAAAGTT CCGTTTGCCT ACTCCTATGG TCAACATTTT GAATGGAGGT AAACACGCAG GTGGTCGTCT CCAAATTCAA GAATTTATGA TTTTACCAAA GGAAAATCAA CCATTCCGTG AGAAGGTGAG ATGTGTCGCT GAAGTTTATC AACATTTGGG TAAGATTCTT GCTGAGAGAG CTGGTCCAAG TGCAAAGAAC GTTGGTGATG AAGGAGGATT TGCACCAAAC TTGGAAACTG CTGATGAAGC ATTGAATTAT ATCGAGGAAG CAATTGGCAA GGCAGGCTAC AAGGTTGGCG AAGATGTGTT TTTGGCTTTG GATGCTGCTT CAAGTGAGTT CTACAACTCG GACACAAAGA AGTATGAAAT TACTCAACAA AAAGAGTTCT TGACCTCCGA AGAAATGGTT GAATATTATG TTCAATTGGT CAATAGACAC CCTGCTATTA TCTCCATTGA AGATGGATTG GAAGAAAAGG ATTATGAAGG ATGGAAACTT TTGACTGAAA GATTAGGATC AAAGATCATG TTGGTTGGTG ATGATTTGTA CACAACAAAT ACCAGATTAA TCAAGCAAGG AATTGAGGAA AAGTGGGCAA ATGCTTTGTT GTTGAAGGTT AACCAAATTG GTACCATCAC CGAAGCAATG AATGCTGCCA GAATGATTTT CAATGTTGGA CAAAAGGTTA TTGTTTCGCA TAGATCCGGT GAGACTGCAA CCACTTTAAT CTCTGACTTG GTGGTTGGTA TTGGAGCCAC ACACATTAAG ACTGGTGCTA CTGCTAGAGG AGAACGTGTA TCCAAGTACA ACCGATTGTT GCAAATCGAA GAATATCTTG AACAACACGG TTTGCTTGCA TGAGTAAGAT AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAATTCT TGAAGACGAA AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTGTT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGCA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CTGATGCGGT ATTTTCTCCT TACGCATCTG TGCGGTATTT CACACCGCAT ATATGGTGCA CTCTCAGTAC AATCTGCTCT GATGCCGCAT AGTTAAGCCA GTATACACTC CGCTATCGCT ACGTGACTGG GTCATGGCTG CGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCGTCTCCG GGAGCTGCAT GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGAGG CAGCTGCGGT AAAGCTCATC AGCGTGGTCG TGAAGCGATT CACAGATGTC TGCCTGTTCA TCCGCGTCCA GCTCGTTGAG TTTCTCCAGA AGCGTTAATG TCTGGCTTCT GATAAAGCGG GCCATGTTAA GGGCGGTTTT TTCCTGTTTG GTCACTGATG CCTCCGTGTA AGGGGGATTT CTGTTCATGG GGGTAATGAT ACCGATGAAA CGAGAGAGGA TGCTCACGAT ACGGGTTACT GATGATGAAC ATGCCCGGTT ACTGGAACGT TGTGAGGGTA AACAACTGGC GGTATGGATG CGGCGGGACC AGAGAAAAAT CACTCAGGGT CAATGCCAGC GCTTCGTTAA TACAGATGTA GGTGTTCCAC AGGGTAGCCA GCAGCATCCT GCGATGCAGA TCCGGAACAT AATGGTGCAG GGCGCTGACT TCCGCGTTTC CAGACTTTAC GAAACACGGA AACCGAAGAC CATTCATGTT GTTGCTCAGG TCGCAGACGT TTTGCAGCAG CAGTCGCTTC ACGTTCGCTC GCGTATCGGT GATTCATTCT GCTAACCAGT AAGGCAACCC CGCCAGCCTA GCCGGGTCCT CAACGACAGG AGCACGATCA TGCGCACCCG TGGCCAGGAC CCAACGCTGC CCGAGATGCG CCGCGTGCGG CTGCTGGAGA TGGCGGACGC GATGGATATG TTCTGCCAAG GGTTGGTTTG CGCATTCACA GTTCTCCGCA AGAATTGATT GGCTCCAATT CTTGGAGTGG TGAATCCGTT AGCGAGGTGC CGCCGGCTTC CATTCAGGTC GAGGTGGCCC GGCTCCATGC ACCGCGACGC AACGCGGGGA GGCAGACAAG GTATAGGGCG GCGCCTACAA TCCATGCCAA CCCGTTCCAT GTGCTCGCCG AGGCGGCATA AATCGCCGTG ACGATCAGCG GTCCAGTGAT CGAAGTTAGG CTGGTAAGAG CCGCGAGCGA TCCTTGAAGC TGTCCCTGAT GGTCGTCATC TACCTGCCTG GACAGCATGG CCTGCAACGC GGGCATCCCG ATGCCGCCGG AAGCGAGAAG AATCATAATG GGGAAGGCCA TCCAGCCTCG CGTCGCGAAC GCCAGCAAGA CGTAGCCCAG CGCGTCGGCC GCCATGCCGG CGATAATGGC CTGCTTCTCG CCGAAACGTT TGGTGGCGGG ACCAGTGACG AAGGCTTGAG CGAGGGCGTG CAAGATTCCG AATACCGCAA GCGACAGGCC GATCATCGTC GCGCTCCAGC GAAAGCGGTC CTCGCCGAAA ATGACCCAGA GCGCTGCCGG CACCTGTCCT ACGAGTTGCA TGATAAAGAA GACAGTCATA AGTGCGGCGA CGATAGTCAT GCCCCGCGCC CACCGGAAGG AGCTGACTGG GTTGAAGGCT CTCAAGGGCA TCGGTCGACG CTCTCCCTTA TGCGACTCCT GCATTAGGAA GCAGCCCAGT AGTAGGTTGA GGCCGTTGAG CACCGCCGCC GCAAGGAATG GTGCATGCAA GGAGATGGCG CCCAACAGTC CCCCGGCCAC GGGGCCTGCC ACCATACCCA CGCCGAAACA AGCGCTCATG AGCCCGAAGT GGCGAGCCCG ATCTTCCCCA TCGGTGATGT CGGCGATATA GGCGCCAGCA ACCGCACCTG TGGCGCCGGT GATGCCGGCC ACGATGCGTC CGGCGTAGAG GATCGAGATC TCGATCCCGC GAAAT
Details for NafoA.00379.a.B5.PW39046
| PURIFICATION DATe: | 6/25/2021 |
| CONCENTRATION: | 73mg/ml |
| OBSERVED MW: | 52kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| VIAL COUNT (approx.): | 4 |
| VIAL VOLUME: | 52µl |
| PERCENT IDENTITY: | 95 |
| PERCENT COVERAGE: | 63 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMQ KLVEDALNEC YNANASDPVG FLGHFFLNRG KKGAVNRVDK LVGREILDSR GNPTVEVDVY ANGQKRPVAT ASAPSGASTG SNEAHELRDG DKSRYLGKGV LKAVKNVNDV LGKAVEGKSL ENLTELDQAL IDADGDELKS NLGGNAITAC SFALATAGAA VRNEELFLYL ARAFHGADKF ENLKFRLPTP MVNILNGGKH AGGRLQIQEF MILPKENQPF REKVRCVAEV YQHLGKILAE RAGPSAKNVG DEGGXAPNLE TADEALNYIE EAIGQGRLXX WRXVFWLXML LQ
Validated NT Sequence
acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta nnaaggagat ataccatggc tcaccaccac caccaccata tgcaaaagtt ggttgaagat gctctcaatg aatgctacaa tgccaacgcc agtgatcccg ttggattcct tggtcacttc tttttgaacc gtggaaagaa gggtgctgtc aatcgtgtcg acaagcttgt cggtagagaa attttggatt caagaggtaa tccaacggtt gaagttgacg tttatgctaa tggacaaaag agaccagtag ccactgcttc tgcaccatcc ggagcaagta caggaagcaa tgaagctcac gaacttcgtg atggtgataa atcaagatac ctcggaaaag gtgttttgaa agctgtgaag aatgtgaatg atgtgttggg caaggcagtg gaaggaaagt cattggaaaa cttgacagaa ctcgatcaag ctttgatcga tgctgatggt gatgagttga agagcaattt gggaggtaat gcgatcactg catgcagttt tgcacttgcc actgctggtg ctgctgtcag aaatgaggaa ttgttcttgt acttggcaag agcattccat ggagctgata agtttgaaaa cttaaagttc cgtttgccta ctcctatggt caacattttg aatggaggta aacacgcagg tggtcgtctc caaattcaag aatttatgat tttaccaaag gaaaatcaac cattccgtga gaaggtgaga tgtgtcgctg aagtttatca acatttgggt aagattcttg ctgagagagc tggtccaagt gcnaagaacg ttggtgatga aggaggantt gcaccnaact tggaaactgc tgatgaagca ttgaattata tcgaggaagc aattgggcaa ggcaggctan naanntggcg anatgtgttt tggctttnga tgctgcttca gtgagttcta nactcggacn ctaanaagta tgaaattant cnncaaaaga tnntgacctn cgaagaannn nnaanntatn tcantngnca annnnnannc cctgctnnnt annnncattg annangggat tgnnnnnnaa a
Expressed Protein Sequence
MAHHHHHHQK LVEDALNECY NANASDPVGF LGHFFLNRGK KGAVNRVDKL VGREILDSRG NPTVEVDVYA NGQKRPVATA SAPSGASTGS NEAHELRDGD KSRYLGKGVL KAVKNVNDVL GKAVEGKSLE NLTELDQALI DADGDELKSN LGGNAITACS FALATAGAAV RNEELFLYLA RAFHGADKFE NLKFRLPTPM VNILNGGKHA GGRLQIQEFM ILPKENQPFR EKVRCVAEVY QHLGKILAER AGPSAKNVGD EGGFAPNLET ADEALNYIEE AIGKAGYKVG EDVFLALDAA SSEFYNSDTK KYEITQQKEF LTSEEMVEYY VQLVNRHPAI ISIEDGLEEK DYEGWKLLTE RLGSKIMLVG DDLYTTNTRL IKQGIEEKWA NALLLKVNQI GTITEAMNAA RMIFNVGQKV IVSHRSGETA TTLISDLVVG IGATHIKTGA TARGERVSKY NRLLQIEEYL EQHGLLA
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATCAAAAGT TGGTTGAAGA TGCTCTCAAT GAATGCTACA ATGCCAACGC CAGTGATCCC GTTGGATTCC TTGGTCACTT CTTTTTGAAC CGTGGAAAGA AGGGTGCTGT CAATCGTGTC GACAAGCTTG TTGGTAGAGA AATTTTGGAT TCAAGAGGTA ATCCAACGGT TGAAGTTGAC GTTTATGCTA ATGGACAAAA GAGACCAGTA GCCACTGCTT CTGCACCATC CGGAGCAAGT ACAGGAAGCA ATGAAGCTCA CGAACTTCGT GATGGTGATA AATCAAGATA CCTCGGAAAA GGTGTTTTGA AAGCTGTGAA GAATGTGAAT GATGTGTTGG GCAAGGCAGT GGAAGGAAAG TCATTGGAAA ACTTGACAGA ACTCGATCAA GCTTTGATCG ATGCTGATGG TGATGAGTTG AAGAGCAATT TGGGAGGTAA TGCGATCACT GCATGCAGTT TTGCACTTGC CACTGCTGGT GCTGCTGTCA GAAATGAGGA ATTGTTCTTG TACTTGGCAA GAGCATTCCA TGGAGCTGAT AAGTTTGAAA ACTTAAAGTT CCGTTTGCCT ACTCCTATGG TCAACATTTT GAATGGAGGT AAACACGCAG GTGGTCGTCT CCAAATTCAA GAATTTATGA TTTTACCAAA GGAAAATCAA CCATTCCGTG AGAAGGTGAG ATGTGTCGCT GAAGTTTATC AACATTTGGG TAAGATTCTT GCTGAGAGAG CTGGTCCAAG TGCAAAGAAC GTTGGTGATG AAGGAGGATT TGCACCAAAC TTGGAAACTG CTGATGAAGC ATTGAATTAT ATCGAGGAAG CAATTGGCAA GGCAGGCTAC AAGGTTGGCG AAGATGTGTT TTTGGCTTTG GATGCTGCTT CAAGTGAGTT CTACAACTCG GACACAAAGA AGTATGAAAT TACTCAACAA AAAGAGTTCT TGACCTCCGA AGAAATGGTT GAATATTATG TTCAATTGGT CAATAGACAC CCTGCTATTA TCTCCATTGA AGATGGATTG GAAGAAAAGG ATTATGAAGG ATGGAAACTT TTGACTGAAA GATTAGGATC AAAGATCATG TTGGTTGGTG ATGATTTGTA CACAACAAAT ACCAGATTAA TCAAGCAAGG AATTGAGGAA AAGTGGGCAA ATGCTTTGTT GTTGAAGGTT AACCAAATTG GTACCATCAC CGAAGCAATG AATGCTGCCA GAATGATTTT CAATGTTGGA CAAAAGGTTA TTGTTTCGCA TAGATCCGGT GAGACTGCAA CCACTTTAAT CTCTGACTTG GTGGTTGGTA TTGGAGCCAC ACACATTAAG ACTGGTGCTA CTGCTAGAGG AGAACGTGTA TCCAAGTACA ACCGATTGTT GCAAATCGAA GAATATCTTG AACAACACGG TTTGCTTGCA TGAGTAAGAT AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAATTCT TGAAGACGAA AGGGCCTCGT GATACGCCTA TTTTTATAGG TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTGTT GACGCCGGGC AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGCA GCAATGGCAA CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA GAGCGCAGAT ACCAAATACT GTCCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGCGC CTGATGCGGT ATTTTCTCCT TACGCATCTG TGCGGTATTT CACACCGCAT ATATGGTGCA CTCTCAGTAC AATCTGCTCT GATGCCGCAT AGTTAAGCCA GTATACACTC CGCTATCGCT ACGTGACTGG GTCATGGCTG CGCCCCGACA CCCGCCAACA CCCGCTGACG CGCCCTGACG GGCTTGTCTG CTCCCGGCAT CCGCTTACAG ACAAGCTGTG ACCGTCTCCG GGAGCTGCAT GTGTCAGAGG TTTTCACCGT CATCACCGAA ACGCGCGAGG CAGCTGCGGT AAAGCTCATC AGCGTGGTCG TGAAGCGATT CACAGATGTC TGCCTGTTCA TCCGCGTCCA GCTCGTTGAG TTTCTCCAGA AGCGTTAATG TCTGGCTTCT GATAAAGCGG GCCATGTTAA GGGCGGTTTT TTCCTGTTTG GTCACTGATG CCTCCGTGTA AGGGGGATTT CTGTTCATGG GGGTAATGAT ACCGATGAAA CGAGAGAGGA TGCTCACGAT ACGGGTTACT GATGATGAAC ATGCCCGGTT ACTGGAACGT TGTGAGGGTA AACAACTGGC GGTATGGATG CGGCGGGACC AGAGAAAAAT CACTCAGGGT CAATGCCAGC GCTTCGTTAA TACAGATGTA GGTGTTCCAC AGGGTAGCCA GCAGCATCCT GCGATGCAGA TCCGGAACAT AATGGTGCAG GGCGCTGACT TCCGCGTTTC CAGACTTTAC GAAACACGGA AACCGAAGAC CATTCATGTT GTTGCTCAGG TCGCAGACGT TTTGCAGCAG CAGTCGCTTC ACGTTCGCTC GCGTATCGGT GATTCATTCT GCTAACCAGT AAGGCAACCC CGCCAGCCTA GCCGGGTCCT CAACGACAGG AGCACGATCA TGCGCACCCG TGGCCAGGAC CCAACGCTGC CCGAGATGCG CCGCGTGCGG CTGCTGGAGA TGGCGGACGC GATGGATATG TTCTGCCAAG GGTTGGTTTG CGCATTCACA GTTCTCCGCA AGAATTGATT GGCTCCAATT CTTGGAGTGG TGAATCCGTT AGCGAGGTGC CGCCGGCTTC CATTCAGGTC GAGGTGGCCC GGCTCCATGC ACCGCGACGC AACGCGGGGA GGCAGACAAG GTATAGGGCG GCGCCTACAA TCCATGCCAA CCCGTTCCAT GTGCTCGCCG AGGCGGCATA AATCGCCGTG ACGATCAGCG GTCCAGTGAT CGAAGTTAGG CTGGTAAGAG CCGCGAGCGA TCCTTGAAGC TGTCCCTGAT GGTCGTCATC TACCTGCCTG GACAGCATGG CCTGCAACGC GGGCATCCCG ATGCCGCCGG AAGCGAGAAG AATCATAATG GGGAAGGCCA TCCAGCCTCG CGTCGCGAAC GCCAGCAAGA CGTAGCCCAG CGCGTCGGCC GCCATGCCGG CGATAATGGC CTGCTTCTCG CCGAAACGTT TGGTGGCGGG ACCAGTGACG AAGGCTTGAG CGAGGGCGTG CAAGATTCCG AATACCGCAA GCGACAGGCC GATCATCGTC GCGCTCCAGC GAAAGCGGTC CTCGCCGAAA ATGACCCAGA GCGCTGCCGG CACCTGTCCT ACGAGTTGCA TGATAAAGAA GACAGTCATA AGTGCGGCGA CGATAGTCAT GCCCCGCGCC CACCGGAAGG AGCTGACTGG GTTGAAGGCT CTCAAGGGCA TCGGTCGACG CTCTCCCTTA TGCGACTCCT GCATTAGGAA GCAGCCCAGT AGTAGGTTGA GGCCGTTGAG CACCGCCGCC GCAAGGAATG GTGCATGCAA GGAGATGGCG CCCAACAGTC CCCCGGCCAC GGGGCCTGCC ACCATACCCA CGCCGAAACA AGCGCTCATG AGCCCGAAGT GGCGAGCCCG ATCTTCCCCA TCGGTGATGT CGGCGATATA GGCGCCAGCA ACCGCACCTG TGGCGCCGGT GATGCCGGCC ACGATGCGTC CGGCGTAGAG GATCGAGATC TCGATCCCGC GAAAT
Details for NafoA.00379.a.B4.PW39047
| PURIFICATION DATe: | 7/22/2021 |
| CONCENTRATION: | 45mg/ml |
| OBSERVED MW: | 50kDa |
| EXPRESSION LEVEL: | High Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol, 2 mM DTT, 0.025% Azide |
| EXPRESSION HOST: | BL 21 (DE3) Rosetta |
| VIAL COUNT (approx.): | 6 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | data unavailable |
| PERCENT COVERAGE: | data unavailable |
Protocol Notes
notes unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expressed Protein Sequence
MAHHHHHHPV GFLGHFFLNR GKKGAVNRVD KLVGREILDS RGNPTVEVDV YANGQKRPVA TASAPSGAST GSNEAHELRD GDKSRYLGKG VLKAVKNVND VLGKAVEGKS LENLTELDQA LIDADGDELK SNLGGNAITA CSFALATAGA AVRNEELFLY LARAFHGADK FENLKFRLPT PMVNILNGGK HAGGRLQIQE FMILPKENQP FREKVRCVAE VYQHLGKILA ERAGPSAKNV GDEGGFAPNL ETADEALNYI EEAIGKAGYK VGEDVFLALD AASSEFYNSD TKKYEITQQK EFLTSEEMVE YYVQLVNRHP AIISIEDGLE EKDYEGWKLL TERLGSKIML VGDDLYTTNT RLIKQGIEEK WANALLLKVN QIGTITEAMN AARMIFNVGQ KVIVSHRSGE TATTLISDLV VGIGATHIKT GATARGERVS KYNRLLQIEE YLEQHGLLA
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATCCCGTTG GATTCCTTGG TCACTTCTTT TTGAACCGTG GAAAGAAGGG TGCTGTCAAT CGTGTCGACA AGCTTGTTGG TAGAGAAATT TTGGATTCAA GAGGTAATCC AACGGTTGAA GTTGACGTTT ATGCTAATGG ACAAAAGAGA CCAGTAGCCA CTGCTTCTGC ACCATCCGGA GCAAGTACAG GAAGCAATGA AGCTCACGAA CTTCGTGATG GTGATAAATC AAGATACCTC GGAAAAGGTG TTTTGAAAGC TGTGAAGAAT GTGAATGATG TGTTGGGCAA GGCAGTGGAA GGAAAGTCAT TGGAAAACTT GACAGAACTC GATCAAGCTT TGATCGATGC TGATGGTGAT GAGTTGAAGA GCAATTTGGG AGGTAATGCG ATCACTGCAT GCAGTTTTGC ACTTGCCACT GCTGGTGCTG CTGTCAGAAA TGAGGAATTG TTCTTGTACT TGGCAAGAGC ATTCCATGGA GCTGATAAGT TTGAAAACTT AAAGTTCCGT TTGCCTACTC CTATGGTCAA CATTTTGAAT GGAGGTAAAC ACGCAGGTGG TCGTCTCCAA ATTCAAGAAT TTATGATTTT ACCAAAGGAA AATCAACCAT TCCGTGAGAA GGTGAGATGT GTCGCTGAAG TTTATCAACA TTTGGGTAAG ATTCTTGCTG AGAGAGCTGG TCCAAGTGCA AAGAACGTTG GTGATGAAGG AGGATTTGCA CCAAACTTGG AAACTGCTGA TGAAGCATTG AATTATATCG AGGAAGCAAT TGGCAAGGCA GGCTACAAGG TTGGCGAAGA TGTGTTTTTG GCTTTGGATG CTGCTTCAAG TGAGTTCTAC AACTCGGACA CAAAGAAGTA TGAAATTACT CAACAAAAAG AGTTCTTGAC CTCCGAAGAA ATGGTTGAAT ATTATGTTCA ATTGGTCAAT AGACACCCTG CTATTATCTC CATTGAAGAT GGATTGGAAG AAAAGGATTA TGAAGGATGG AAACTTTTGA CTGAAAGATT AGGATCAAAG ATCATGTTGG TTGGTGATGA TTTGTACACA ACAAATACCA GATTAATCAA GCAAGGAATT GAGGAAAAGT GGGCAAATGC TTTGTTGTTG AAGGTTAACC AAATTGGTAC CATCACCGAA GCAATGAATG CTGCCAGAAT GATTTTCAAT GTTGGACAAA AGGTTATTGT TTCGCATAGA TCCGGTGAGA CTGCAACCAC TTTAATCTCT GACTTGGTGG TTGGTATTGG AGCCACACAC ATTAAGACTG GTGCTACTGC TAGAGGAGAA CGTGTATCCA AGTACAACCG ATTGTTGCAA ATCGAAGAAT ATCTTGAACA ACACGGTTTG CTTGCATGAG TAAGATAGGA TCCGGCTGCT AACAAAGCCC GAAAGGAAGC TGAGTTGGCT GCTGCCACCG CTGAGCAATA ACTAGCATAA CCCCTTGGGG CCTCTAAACG GGTCTTGAGG GGTTTTTTGC TGAAAGGAGG AACTATATCC GGATATCCAC AGGACGGGTG TGGTCGCCAT GATCGCGTAG TCGATAGTGG CTCCAAGTAG CGAAGCGAGC AGGACTGGGC GGCGGCCAAA GCGGTCGGAC AGTGCTCCGA GAACGGGTGC GCATAGAAAT TGCATCAACG CATATAGCGC TAGCAGCACG CCATAGTGAC TGGCGATGCT GTCGGAATGG ACGATATCCC GCAAGAGGCC CGGCAGTACC GGCATAACCA AGCCTATGCC TACAGCATCC AGGGTGACGG TGCCGAGGAT GACGATGAGC GCATTGTTAG ATTTCATACA CGGTGCCTGA CTGCGTTAGC AATTTAACTG TGATAAACTA CCGCATTAAA GCTTATCGAT GATAAGCTGT CAAACATGAG AATTCTTGAA GACGAAAGGG CCTCGTGATA CGCCTATTTT TATAGGTTAA TGTCATGATA ATAATGGTTT CTTAGACGTC AGGTGGCACT TTTCGGGGAA ATGTGCGCGG AACCCCTATT TGTTTATTTT TCTAAATACA TTCAAATATG TATCCGCTCA TGAGACAATA ACCCTGATAA ATGCTTCAAT AATATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTGTTGACG CCGGGCAAGA GCAACTCGGT CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC ATACCAAACG ACGAGCGTGA CACCACGATG CCTGCAGCAA TGGCAACAAC GTTGCGCAAA CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG CGCGTAATCT GCTGCTTGCA AACAAAAAAA CCACCGCTAC CAGCGGTGGT TTGTTTGCCG GATCAAGAGC TACCAACTCT TTTTCCGAAG GTAACTGGCT TCAGCAGAGC GCAGATACCA AATACTGTCC TTCTAGTGTA GCCGTAGTTA GGCCACCACT TCAAGAACTC TGTAGCACCG CCTACATACC TCGCTCTGCT AATCCTGTTA CCAGTGGCTG CTGCCAGTGG CGATAAGTCG TGTCTTACCG GGTTGGACTC AAGACGATAG TTACCGGATA AGGCGCAGCG GTCGGGCTGA ACGGGGGGTT CGTGCACACA GCCCAGCTTG GAGCGAACGA CCTACACCGA ACTGAGATAC CTACAGCGTG AGCTATGAGA AAGCGCCACG CTTCCCGAAG GGAGAAAGGC GGACAGGTAT CCGGTAAGCG GCAGGGTCGG AACAGGAGAG CGCACGAGGG AGCTTCCAGG GGGAAACGCC TGGTATCTTT ATAGTCCTGT CGGGTTTCGC CACCTCTGAC TTGAGCGTCG ATTTTTGTGA TGCTCGTCAG GGGGGCGGAG CCTATGGAAA AACGCCAGCA ACGCGGCCTT TTTACGGTTC CTGGCCTTTT GCTGGCCTTT TGCTCACATG TTCTTTCCTG CGTTATCCCC TGATTCTGTG GATAACCGTA TTACCGCCTT TGAGTGAGCT GATACCGCTC GCCGCAGCCG AACGACCGAG CGCAGCGAGT CAGTGAGCGA GGAAGCGGAA GAGCGCCTGA TGCGGTATTT TCTCCTTACG CATCTGTGCG GTATTTCACA CCGCATATAT GGTGCACTCT CAGTACAATC TGCTCTGATG CCGCATAGTT AAGCCAGTAT ACACTCCGCT ATCGCTACGT GACTGGGTCA TGGCTGCGCC CCGACACCCG CCAACACCCG CTGACGCGCC CTGACGGGCT TGTCTGCTCC CGGCATCCGC TTACAGACAA GCTGTGACCG TCTCCGGGAG CTGCATGTGT CAGAGGTTTT CACCGTCATC ACCGAAACGC GCGAGGCAGC TGCGGTAAAG CTCATCAGCG TGGTCGTGAA GCGATTCACA GATGTCTGCC TGTTCATCCG CGTCCAGCTC GTTGAGTTTC TCCAGAAGCG TTAATGTCTG GCTTCTGATA AAGCGGGCCA TGTTAAGGGC GGTTTTTTCC TGTTTGGTCA CTGATGCCTC CGTGTAAGGG GGATTTCTGT TCATGGGGGT AATGATACCG ATGAAACGAG AGAGGATGCT CACGATACGG GTTACTGATG ATGAACATGC CCGGTTACTG GAACGTTGTG AGGGTAAACA ACTGGCGGTA TGGATGCGGC GGGACCAGAG AAAAATCACT CAGGGTCAAT GCCAGCGCTT CGTTAATACA GATGTAGGTG TTCCACAGGG TAGCCAGCAG CATCCTGCGA TGCAGATCCG GAACATAATG GTGCAGGGCG CTGACTTCCG CGTTTCCAGA CTTTACGAAA CACGGAAACC GAAGACCATT CATGTTGTTG CTCAGGTCGC AGACGTTTTG CAGCAGCAGT CGCTTCACGT TCGCTCGCGT ATCGGTGATT CATTCTGCTA ACCAGTAAGG CAACCCCGCC AGCCTAGCCG GGTCCTCAAC GACAGGAGCA CGATCATGCG CACCCGTGGC CAGGACCCAA CGCTGCCCGA GATGCGCCGC GTGCGGCTGC TGGAGATGGC GGACGCGATG GATATGTTCT GCCAAGGGTT GGTTTGCGCA TTCACAGTTC TCCGCAAGAA TTGATTGGCT CCAATTCTTG GAGTGGTGAA TCCGTTAGCG AGGTGCCGCC GGCTTCCATT CAGGTCGAGG TGGCCCGGCT CCATGCACCG CGACGCAACG CGGGGAGGCA GACAAGGTAT AGGGCGGCGC CTACAATCCA TGCCAACCCG TTCCATGTGC TCGCCGAGGC GGCATAAATC GCCGTGACGA TCAGCGGTCC AGTGATCGAA GTTAGGCTGG TAAGAGCCGC GAGCGATCCT TGAAGCTGTC CCTGATGGTC GTCATCTACC TGCCTGGACA GCATGGCCTG CAACGCGGGC ATCCCGATGC CGCCGGAAGC GAGAAGAATC ATAATGGGGA AGGCCATCCA GCCTCGCGTC GCGAACGCCA GCAAGACGTA GCCCAGCGCG TCGGCCGCCA TGCCGGCGAT AATGGCCTGC TTCTCGCCGA AACGTTTGGT GGCGGGACCA GTGACGAAGG CTTGAGCGAG GGCGTGCAAG ATTCCGAATA CCGCAAGCGA CAGGCCGATC ATCGTCGCGC TCCAGCGAAA GCGGTCCTCG CCGAAAATGA CCCAGAGCGC TGCCGGCACC TGTCCTACGA GTTGCATGAT AAAGAAGACA GTCATAAGTG CGGCGACGAT AGTCATGCCC CGCGCCCACC GGAAGGAGCT GACTGGGTTG AAGGCTCTCA AGGGCATCGG TCGACGCTCT CCCTTATGCG ACTCCTGCAT TAGGAAGCAG CCCAGTAGTA GGTTGAGGCC GTTGAGCACC GCCGCCGCAA GGAATGGTGC ATGCAAGGAG ATGGCGCCCA ACAGTCCCCC GGCCACGGGG CCTGCCACCA TACCCACGCC GAAACAAGCG CTCATGAGCC CGAAGTGGCG AGCCCGATCT TCCCCATCGG TGATGTCGGC GATATAGGCG CCAGCAACCG CACCTGTGGC GCCGGTGATG CCGGCCACGA TGCGTCCGGC GTAGAGGATC GAGATCTCGA TCCCGCGAAA T