NafoA.00134.a

4-hydroxyphenylpyruvate dioxygenase

CENTER ID:	NafoA.00134.a
ORGANISM:	Naegleria fowleri ATCC 30863
ASSOCIATED DISEASE:
CURRENT STATUS:	purified
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
NafoA.00134.a.B1.GE40220	Full length(NafoA.00134.a)		1	393

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
NafoA.00134.a.B1.PS38151	Full length(NafoA.00134.a)		1	393

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	AmoebaDB:NF0084900

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MMSAQVPAGR FLSFDYVEFW VGNALQARDY YISRFGFRPY AYRGLETGSK EIVTHVVRQN DAILAFSSPL LPASKSALAR EMTAHQAEHG DGVRDIAFTV DNAEAIYKAA VENGAISVRE PQTLTDPELG SVKIASVKTY GDTIHTFVER SNFKGKFLPG FKVIEQDGDD PFQSFTPEVG LLNIDHIVGN QGWNEMVNIC EWYEQKLGFH RFWSVDDSVI HTEYSALKSI VMTDKEENIK MPINEPAKGK KMSQIEEFVN FYGGAGAQHI ALRTNDIIHA VTQMRARGVS FLPTPDSYYD NLRKRLAHAP ITVKEDIDVL QKLKILVDFD DKGYLLQIFT KPVEDRPTLF YEIIQRQNNS GFGAGNFKAL FESIEHEQER RGTLVDTNYT IQC

NT Sequence

ATGATGTCCG CTCAAGTCCC TGCTGGTCGA TTCTTGTCCT TTGATTACGT TGAATTTTGG GTTGGAAATG CCCTTCAAGC TCGTGATTAC TACATTTCAA GATTTGGATT CCGACCATAT GCCTATCGTG GTTTAGAAAC TGGAAGTAAG GAGATTGTCA CTCATGTCGT TCGTCAAAAC GATGCCATTC TCGCCTTCTC TTCTCCTCTC TTACCTGCTT CAAAGAGTGC ATTGGCACGA GAGATGACTG CTCACCAAGC TGAACACGGA GATGGTGTTC GTGATATTGC CTTCACTGTG GATAATGCAG AGGCCATTTA TAAGGCAGCT GTTGAGAATG GAGCAATTAG TGTGAGAGAA CCACAAACTT TGACAGATCC AGAATTGGGA AGTGTCAAAA TTGCAAGTGT TAAAACTTAT GGAGATACTA TTCACACCTT TGTGGAGAGA TCAAACTTCA AGGGAAAGTT CTTACCTGGA TTTAAGGTGA TTGAACAAGA TGGTGATGAT CCATTCCAAA GCTTTACTCC TGAAGTTGGA TTGCTGAATA TTGATCATAT TGTCGGAAAC CAAGGATGGA ATGAAATGGT GAACATTTGT GAATGGTATG AACAAAAATT GGGTTTCCAT CGATTCTGGA GTGTCGATGA CTCTGTCATT CACACCGAAT ACAGTGCTTT GAAGAGTATT GTCATGACTG ATAAGGAGGA AAACATCAAA ATGCCAATCA ATGAACCTGC AAAGGGAAAG AAAATGTCAC AAATTGAAGA GTTTGTAAAC TTTTATGGAG GTGCAGGTGC TCAACACATT GCTTTGAGAA CCAATGATAT CATTCATGCA GTGACTCAAA TGAGAGCTCG TGGTGTGAGC TTTTTGCCAA CTCCAGATTC GTATTATGAC AATTTGAGAA AGAGATTGGC TCATGCTCCA ATTACTGTTA AGGAAGATAT TGATGTTTTG CAAAAGTTAA AGATTTTGGT TGACTTTGAT GATAAGGGAT ACTTGTTGCA AATTTTCACC AAGCCAGTGG AAGATAGACC AACCTTGTTC TATGAAATTA TTCAAAGACA AAATAATAGT GGATTTGGAG CTGGTAACTT TAAGGCACTT TTCGAATCCA TTGAACATGA ACAAGAGAGA CGTGGTACAT TGGTCGATAC CAACTACACC ATTCAATGT

Details for NafoA.00134.a.B1.GE40220

HARVESTED ON:	5/9/2016
SEQUENCED ON:	5/23/2016
EXPECTED MW:	45kDa
OBSERVED MW:	46kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	Moderate Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Validated AA Sequence

MAHHHHHHMM SAQVPAGRFL SFDYVEFWVG NALQARDYYI SRFGFRPYAY RGLETGSKEI VTHVVRQNDA ILAFSSPLLP ASKSALAREM TAHQAEHGDG VRDIAFTVDN AEAIYKAAVE NGAISVREPQ TLTDPELGSV KIASVKTYGD TIHTFVERSN FKGKFLPGFK VIEQDGDDPF QSFTPEVGLL NIDHIVGNQG WNEMVNICEW YEQKLGFHRF WSVDDSVIHT EYSALKSIVM TDKEENIKMP INEPAKGKKM SQIEEFVNFY GGAGAQHIAL RTNDIIHAVT QMRARGVSFL PTPDSYYDNL RKRLAHAPIT VKEDIDVLQK LKILVDFDDK GYLLQIFTKP VEDRPTLFYE IIQRQNNSGF GAGNFKALFE SIEHEQERRG TL

Validated NT Sequence

ccaatgtacc acgtctctct tgttcatgtt caatggattc gaaaagtgcc ttaaagttac cagctccaaa tccactatta ttttgtcttt gaataatttc atagaacaag gttggtctat cttccactgg cttggtgaaa atttgcaaca agtatccctt atcatcaaag tcaaccaaaa tctttaactt ttgcaaaaca tcaatatctt ccttaacagt aattggagca tgagccaatc tctttctcaa attgtcataa tacgaatctg gagttggcaa aaagctcaca ccacgagctc tcatttgagt cactgcatga atgatatcat tggttctcaa agcaatgtgt tgagcacctg cacctccata aaagtttaca aactcttcaa tttgtgacat tttctttccc tttgcaggtt cattgattgg cattttgatg ttttcctcct tatcagtcat gacaatactc ttcaaagcac tgtattcggt gtgaatgaca gagtcatcga cactccagaa tcgatggaaa cccaattttt gttcatacca ttcacaaatg ttcaccattt cattccatcc ttggtttccg acaatatgat caatattcag caatccaact tcaggagtaa agctttggaa tggatcatca ccatcttgtt caatcacctt aaatccaggt aagaactttc ccttgaagtt tgatctctcc acaaaggtgt gaatagtatc tccataagtt ttaacacttg caattttgac acttcccaat tctggatctg tcaaagtttg tggttctctc acactaattg ctccattctc aacagctgcc ttataaatgg cctctgcatt atccacagtg aaggcaatat cacgaacacc atctccgtgt tcagcttggt gagcagtcat ctctcgtgcc aatgcactct ttgaagcagg taagagagga gaagagaagg cgagaatggc atcgttttga cgaacgacat gagtgacaat ctccttactt ccagtttcta aaccacgata ggcatatggt cggaatccaa atcttgaaat gtagtaatca cgagcttgaa gggcatttcc aacccaaaat tcaacgtaat caaaggacaa gaatcgacca gcagggactt gagcggacat catatggtgg tggtggtggt gagccat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgatgt ccgctcaagt ccctgctggt cgattcttgt cctttgatta cgttgaattt tgggttggaa atgcccttca agctcgtgat tactacattt caagatttgg attccgacca tatgcctatc gtggtttaga aactggaagt aaggagattg tcactcatgt cgttcgtcaa aacgatgcca ttctcgcctt ctcttctcct ctcttacctg cttcaaagag tgcattggca cgagagatga ctgctcacca agctgaacac ggagatggtg ttcgtgatat tgccttcact gtggataatg cagaggccat ttataaggca gctgttgaga atggagcaat tagtgtgaga gaaccacaaa ctttgacaga tccagaattg ggaagtgtca aaattgcaag tgttaaaact tatggagata ctattcacac ctttgtggag agatcaaact tcaagggaaa gttcttacct ggatttaagg tgattgaaca agatggtgat gatccattcc aaagctttac tcctgaagtt ggattgctga atattgatca tattgtcgga aaccaaggat ggaatgaaat ggtgaacatt tgtgaatggt atgaacaaaa attgggtttc catcgattct ggagtgtcga tgactctgtc attcacaccg aatacagtgc tttgaagagt attgtcatga ctgataagga ggaaaacatc aaaatgccaa tcaatgaacc tgcaaaggga aagaaaatgt cacaaattga agagtttgta aacttttatg gaggtgcagg tgctcaacac attgctttga gaaccaatga tatcattcat gcagtgactc aaatgagagc tcgtggtgtg agctttttgc caactccaga ttcgtattat gacaatttga gaaagagatt ggctcatgct ccaattactg ttaaggaaga tattgatgtt ttgcaaaagt taaagatttt ggttgacttt gatgataagg gatacttgtt gcaaattttc accaagccag tggaagatag accaaccttg ttctatgaaa ttattcaaag acaaaataat agtggatttg gagctggtaa ctttaaggca cttttcgaat ccattgaaca tgaacaagag agacgtggta cattggtcga taccaactac accattcaat gttgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat

Details for NafoA.00134.a.B1.PS38151

PURIFICATION DATe:	12/14/2016
CONCENTRATION:	10.5mg/ml
OBSERVED MW:	37kDa
EXPRESSION LEVEL:	Moderate Expression
PROTEIN PURIFICATION BUFFER:	SEC
EXPRESSION HOST:	data unavailable
VIAL COUNT (approx.):	5
VIAL VOLUME:	110µl
PERCENT IDENTITY:	100
PERCENT COVERAGE:	97

Protocol Notes

notes unavailable

Validated AA Sequence

Validated NT Sequence

Expressed Protein Sequence

Full NT Sequence (Expression Vector + Insert)

TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGATGT CCGCTCAAGT CCCTGCTGGT CGATTCTTGT CCTTTGATTA CGTTGAATTT TGGGTTGGAA ATGCCCTTCA AGCTCGTGAT TACTACATTT CAAGATTTGG ATTCCGACCA TATGCCTATC GTGGTTTAGA AACTGGAAGT AAGGAGATTG TCACTCATGT CGTTCGTCAA AACGATGCCA TTCTCGCCTT CTCTTCTCCT CTCTTACCTG CTTCAAAGAG TGCATTGGCA CGAGAGATGA CTGCTCACCA AGCTGAACAC GGAGATGGTG TTCGTGATAT TGCCTTCACT GTGGATAATG CAGAGGCCAT TTATAAGGCA GCTGTTGAGA ATGGAGCAAT TAGTGTGAGA GAACCACAAA CTTTGACAGA TCCAGAATTG GGAAGTGTCA AAATTGCAAG TGTTAAAACT TATGGAGATA CTATTCACAC CTTTGTGGAG AGATCAAACT TCAAGGGAAA GTTCTTACCT GGATTTAAGG TGATTGAACA AGATGGTGAT GATCCATTCC AAAGCTTTAC TCCTGAAGTT GGATTGCTGA ATATTGATCA TATTGTCGGA AACCAAGGAT GGAATGAAAT GGTGAACATT TGTGAATGGT ATGAACAAAA ATTGGGTTTC CATCGATTCT GGAGTGTCGA TGACTCTGTC ATTCACACCG AATACAGTGC TTTGAAGAGT ATTGTCATGA CTGATAAGGA GGAAAACATC AAAATGCCAA TCAATGAACC TGCAAAGGGA AAGAAAATGT CACAAATTGA AGAGTTTGTA AACTTTTATG GAGGTGCAGG TGCTCAACAC ATTGCTTTGA GAACCAATGA TATCATTCAT GCAGTGACTC AAATGAGAGC TCGTGGTGTG AGCTTTTTGC CAACTCCAGA TTCGTATTAT GACAATTTGA GAAAGAGATT GGCTCATGCT CCAATTACTG TTAAGGAAGA TATTGATGTT TTGCAAAAGT TAAAGATTTT GGTTGACTTT GATGATAAGG GATACTTGTT GCAAATTTTC ACCAAGCCAG TGGAAGATAG ACCAACCTTG TTCTATGAAA TTATTCAAAG ACAAAATAAT AGTGGATTTG GAGCTGGTAA CTTTAAGGCA CTTTTCGAAT CCATTGAACA TGAACAAGAG AGACGTGGTA CATTGGTCGA TACCAACTAC ACCATTCAAT GTTGAGTAAG ATAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAATT CTTGAAGACG AAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTG TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG CAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCTGATGCG GTATTTTCTC CTTACGCATC TGTGCGGTAT TTCACACCGC ATATATGGTG CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGTATACAC TCCGCTATCG CTACGTGACT GGGTCATGGC TGCGCCCCGA CACCCGCCAA CACCCGCTGA CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC ATCCGCTTAC AGACAAGCTG TGACCGTCTC CGGGAGCTGC ATGTGTCAGA GGTTTTCACC GTCATCACCG AAACGCGCGA GGCAGCTGCG GTAAAGCTCA TCAGCGTGGT CGTGAAGCGA TTCACAGATG TCTGCCTGTT CATCCGCGTC CAGCTCGTTG AGTTTCTCCA GAAGCGTTAA TGTCTGGCTT CTGATAAAGC GGGCCATGTT AAGGGCGGTT TTTTCCTGTT TGGTCACTGA TGCCTCCGTG TAAGGGGGAT TTCTGTTCAT GGGGGTAATG ATACCGATGA AACGAGAGAG GATGCTCACG ATACGGGTTA CTGATGATGA ACATGCCCGG TTACTGGAAC GTTGTGAGGG TAAACAACTG GCGGTATGGA TGCGGCGGGA CCAGAGAAAA ATCACTCAGG GTCAATGCCA GCGCTTCGTT AATACAGATG TAGGTGTTCC ACAGGGTAGC CAGCAGCATC CTGCGATGCA GATCCGGAAC ATAATGGTGC AGGGCGCTGA CTTCCGCGTT TCCAGACTTT ACGAAACACG GAAACCGAAG ACCATTCATG TTGTTGCTCA GGTCGCAGAC GTTTTGCAGC AGCAGTCGCT TCACGTTCGC TCGCGTATCG GTGATTCATT CTGCTAACCA GTAAGGCAAC CCCGCCAGCC TAGCCGGGTC CTCAACGACA GGAGCACGAT CATGCGCACC CGTGGCCAGG ACCCAACGCT GCCCGAGATG CGCCGCGTGC GGCTGCTGGA GATGGCGGAC GCGATGGATA TGTTCTGCCA AGGGTTGGTT TGCGCATTCA CAGTTCTCCG CAAGAATTGA TTGGCTCCAA TTCTTGGAGT GGTGAATCCG TTAGCGAGGT GCCGCCGGCT TCCATTCAGG TCGAGGTGGC CCGGCTCCAT GCACCGCGAC GCAACGCGGG GAGGCAGACA AGGTATAGGG CGGCGCCTAC AATCCATGCC AACCCGTTCC ATGTGCTCGC CGAGGCGGCA TAAATCGCCG TGACGATCAG CGGTCCAGTG ATCGAAGTTA GGCTGGTAAG AGCCGCGAGC GATCCTTGAA GCTGTCCCTG ATGGTCGTCA TCTACCTGCC TGGACAGCAT GGCCTGCAAC GCGGGCATCC CGATGCCGCC GGAAGCGAGA AGAATCATAA TGGGGAAGGC CATCCAGCCT CGCGTCGCGA ACGCCAGCAA GACGTAGCCC AGCGCGTCGG CCGCCATGCC GGCGATAATG GCCTGCTTCT CGCCGAAACG TTTGGTGGCG GGACCAGTGA CGAAGGCTTG AGCGAGGGCG TGCAAGATTC CGAATACCGC AAGCGACAGG CCGATCATCG TCGCGCTCCA GCGAAAGCGG TCCTCGCCGA AAATGACCCA GAGCGCTGCC GGCACCTGTC CTACGAGTTG CATGATAAAG AAGACAGTCA TAAGTGCGGC GACGATAGTC ATGCCCCGCG CCCACCGGAA GGAGCTGACT GGGTTGAAGG CTCTCAAGGG CATCGGTCGA CGCTCTCCCT TATGCGACTC CTGCATTAGG AAGCAGCCCA GTAGTAGGTT GAGGCCGTTG AGCACCGCCG CCGCAAGGAA TGGTGCATGC AAGGAGATGG CGCCCAACAG TCCCCCGGCC ACGGGGCCTG CCACCATACC CACGCCGAAA CAAGCGCTCA TGAGCCCGAA GTGGCGAGCC CGATCTTCCC CATCGGTGAT GTCGGCGATA TAGGCGCCAG CAACCGCACC TGTGGCGCCG GTGATGCCGG CCACGATGCG TCCGGCGTAG AGGATCGAGA TCTCGATCCC GCGAAAT