NafoA.00085.b
gdp-mannose-dehydratase
| CENTER ID: | NafoA.00085.b |
| ORGANISM: | Naegleria fowleri ATCC 30863 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| NafoA.00085.b.B1.GE40142 | Full length(NafoA.00085.b) | 1 | 380 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| NafoA.00085.b.B1.PS37983 | Full length(NafoA.00085.b) | 1 | 380 |
Structures
5UZH
DEPOSITED: 2/26/2017
DETERMINATION: XRay
CLONE: NafoA.00085.b.B1.GE40142
PROTEIN: NafoA.00085.b.B1.PS37983
Publications by SSGCID
Naegleria fowleri: Protein structures to facilitate drug discovery for the deadly, pathogenic free-living amoeba
Abendroth J, Barrett LK, Dranow DM, Edwards TE, Myler PJ, Phan IQ, Staker B, Subramanian S, Tran N, Van Voorhis WC, Craig JC, Smith C, Chun IKH, Kyle D, Barrett KF, Morris JC, Tillery L, Rice CA, Goldstein J, Lassner JW, Xu L, Young RM, Osterhout B, Leahy JW, Manetsch R
PLoS ONE - 2021
volume 16, issue 3, pages e0241738
PMID: 33760815; PMCID: PMC7990177
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| EuPathDB: | AmoebaDB:NF0016040 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSNSSQTSQE VYDITQVNHP TSTESKVALI TGITGQDGSY LAEFLLEKGY MVYGIIRRSS SFNTGRVEHL YKDIHITKAK FKLLYGDLTD TGNLISIIAK IKPDEIYNLA AQSHVKVSFE MPEYTANVDG IGTLRLLEAI RACGLEKKTK FYQASTSELY GLVQEVPQKE TTPFYPRSPY ACAKLYSYWI VVNYREAYNM FALNGILFNH ESIRRGPTFV TRKITMAVAR IKLGLQDCLY LGNLDAERDW GHAKDYVEAM WLMLQQEQPR DFCVATGEKH SVREFVEKAF ACIGQTVEWK GERGTVEEHG VVDGVVRVRV DPRYFRPTEV DQLLGDPTLA ETVLGWKRKV SFEELVRGMV EGDIELLQSG DPQVVQQQYH
NT Sequence
ATGAGCAATT CATCACAAAC CTCGCAAGAA GTCTATGACA TTACCCAAGT GAATCACCCA ACCAGCACCG AATCCAAGGT GGCCTTGATT ACAGGTATTA CAGGACAAGA TGGTTCCTAT CTTGCTGAAT TTTTGTTGGA AAAGGGTTAT ATGGTGTATG GTATCATTAG ACGTTCTTCA TCCTTCAACA CTGGAAGAGT TGAACACTTG TACAAGGATA TTCATATTAC TAAAGCAAAA TTCAAACTTT TATATGGAGA CTTGACCGAT ACCGGAAACT TAATTTCCAT CATTGCCAAA ATTAAGCCAG ATGAAATTTA CAATTTGGCT GCTCAATCTC ATGTGAAAGT CAGTTTTGAA ATGCCTGAAT ATACTGCTAA TGTTGATGGT ATTGGTACTT TACGTTTGTT GGAAGCAATT CGTGCCTGTG GATTGGAAAA GAAAACCAAA TTTTATCAAG CCAGTACAAG TGAATTGTAC GGTCTAGTGC AAGAGGTTCC TCAAAAGGAA ACAACACCAT TCTATCCTCG TTCACCATAT GCCTGTGCCA AATTGTACAG CTATTGGATT GTTGTTAACT ATAGAGAAGC TTACAACATG TTTGCATTGA ATGGTATTTT GTTCAACCAC GAATCGATTC GTCGTGGTCC AACATTTGTG ACCAGAAAGA TCACCATGGC TGTTGCCCGT ATAAAACTTG GTTTGCAAGA TTGTTTGTAT TTGGGTAACT TGGATGCTGA AAGAGATTGG GGTCATGCTA AGGATTATGT TGAAGCCATG TGGCTCATGT TACAGCAAGA GCAACCACGT GATTTTTGCG TGGCAACTGG TGAAAAACAT AGCGTCAGAG AATTTGTTGA AAAGGCCTTT GCATGCATTG GCCAAACCGT GGAATGGAAA GGAGAAAGAG GAACTGTTGA AGAGCACGGT GTTGTTGATG GTGTTGTAAG AGTTCGAGTC GATCCTCGTT ATTTCCGTCC AACTGAGGTC GATCAATTAT TGGGAGACCC AACTTTGGCT GAGACAGTTT TGGGTTGGAA GAGAAAGGTC AGTTTCGAAG AATTGGTCCG AGGAATGGTG GAAGGAGATA TTGAATTGTT GCAATCTGGA GATCCTCAAG TGGTTCAACA ACAATATCAT
Details for NafoA.00085.b.B1.GE40142
| HARVESTED ON: | 4/6/2016 |
| SEQUENCED ON: | 4/13/2016 |
| EXPECTED MW: | 44kDa |
| OBSERVED MW: | 48kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Many (50-100) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 97 |
Validated AA Sequence
MAHHHHHHMS NSSQTSQEVY DITXXNHPTS TESKVALITG ITGQDGSYLA EFLLEKGYMV YGIIRRSSSF NTGRVEHLYK DIHITKAKFK LLYGDLTDTG NLISIIAKIK PDEIYNLAAQ SHVKVSFEMP EYTANVDGIG TLRLLEAIRA CGLEKKTKFY QASTSELYGL VQEVPQKETT PFYPRSPYAC AKLYSYWIVV NYREAYNMFA LNGILFNHES IRRGPTFVTR KITMAVARIK LGLQDCLYLG NLDAERDWGH AKDYVEAMWL MLQQEQPRDF CVATGEKHSV REFVEKAFAC IGQTVEWKGE RGTVEEHGVV DGVVRVRVDP RYFRPTEVDQ LLGDPTLAET VLGWKRKVSF EELVRGMVEG DIELLQSGDP
Validated NT Sequence
tgaggatctc cagattgcaa caattcaata tctccttcca ccattcctcg gaccaattct tcgaaactga cctttctctt ccaacccaaa actgtctcag ccaaagttgg gtctcccaat aattgatcga cctcagttgg acggaaataa cgaggatcga ctcgaactct tacaacacca tcaacaacac cgtgctcttc aacagttcct ctttctcctt tccattccac ggtttggcca atgcatgcaa aggccttttc aacaaattct ctgacgctat gtttttcacc agttgccacg caaaaatcac gtggttgctc ttgctgtaac atgagccaca tggcttcaac ataatcctta gcatgacccc aatctctttc agcatccaag ttacccaaat acaaacaatc ttgcaaacca agttttatac gggcaacagc catggtgatc tttctggtca caaatgttgg accacgacga atcgattcgt ggttgaacaa aataccattc aatgcaaaca tgttgtaagc ttctctatag ttaacaacaa tccaatagct gtacaatttg gcacaggcat atggtgaacg aggatagaat ggtgttgttt ccttttgagg aacctcttgc actagaccgt acaattcact tgtactggct tgataaaatt tggttttctt ttccaatcca caggcacgaa ttgcttccaa caaacgtaaa gtaccaatac catcaacatt agcagtatat tcaggcattt caaaactgac tttcacatga gattgagcag ccaaattgta aatttcatct ggcttaattt tggcaatgat ggaaattaag tttccggtat cggtcaagtc tccatataaa agtttgaatt ttgctttagt aatatgaata tccttgtaca agtgttcaac tcttccagtg ttgaaggatg aagaacgtct aatgatacca tacaccatat aacccttttc caacaaaaat tcagcaagat aggaaccatc ttgtcctgta atacctgtaa tcaaggccac cttggattcg gtgctggttg ggtgattcan nnnggtaatg tcatagactt cttgcgaggt ttgtgatgaa ttgctcatat ggtggtggtg gtggtgagcc at
Expected Protein Sequence
MAHHHHHHMS NSSQTSQEVY DITQVNHPTS TESKVALITG ITGQDGSYLA EFLLEKGYMV YGIIRRSSSF NTGRVEHLYK DIHITKAKFK LLYGDLTDTG NLISIIAKIK PDEIYNLAAQ SHVKVSFEMP EYTANVDGIG TLRLLEAIRA CGLEKKTKFY QASTSELYGL VQEVPQKETT PFYPRSPYAC AKLYSYWIVV NYREAYNMFA LNGILFNHES IRRGPTFVTR KITMAVARIK LGLQDCLYLG NLDAERDWGH AKDYVEAMWL MLQQEQPRDF CVATGEKHSV REFVEKAFAC IGQTVEWKGE RGTVEEHGVV DGVVRVRVDP RYFRPTEVDQ LLGDPTLAET VLGWKRKVSF EELVRGMVEG DIELLQSGDP QVVQQQYH
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagca attcatcaca aacctcgcaa gaagtctatg acattaccca agtgaatcac ccaaccagca ccgaatccaa ggtggccttg attacaggta ttacaggaca agatggttcc tatcttgctg aatttttgtt ggaaaagggt tatatggtgt atggtatcat tagacgttct tcatccttca acactggaag agttgaacac ttgtacaagg atattcatat tactaaagca aaattcaaac ttttatatgg agacttgacc gataccggaa acttaatttc catcattgcc aaaattaagc cagatgaaat ttacaatttg gctgctcaat ctcatgtgaa agtcagtttt gaaatgcctg aatatactgc taatgttgat ggtattggta ctttacgttt gttggaagca attcgtgcct gtggattgga aaagaaaacc aaattttatc aagccagtac aagtgaattg tacggtctag tgcaagaggt tcctcaaaag gaaacaacac cattctatcc tcgttcacca tatgcctgtg ccaaattgta cagctattgg attgttgtta actatagaga agcttacaac atgtttgcat tgaatggtat tttgttcaac cacgaatcga ttcgtcgtgg tccaacattt gtgaccagaa agatcaccat ggctgttgcc cgtataaaac ttggtttgca agattgtttg tatttgggta acttggatgc tgaaagagat tggggtcatg ctaaggatta tgttgaagcc atgtggctca tgttacagca agagcaacca cgtgattttt gcgtggcaac tggtgaaaaa catagcgtca gagaatttgt tgaaaaggcc tttgcatgca ttggccaaac cgtggaatgg aaaggagaaa gaggaactgt tgaagagcac ggtgttgttg atggtgttgt aagagttcga gtcgatcctc gttatttccg tccaactgag gtcgatcaat tattgggaga cccaactttg gctgagacag ttttgggttg gaagagaaag gtcagtttcg aagaattggt ccgaggaatg gtggaaggag atattgaatt gttgcaatct ggagatcctc aagtggttca acaacaatat cattgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat
Details for NafoA.00085.b.B1.PS37983
| PURIFICATION DATe: | 7/21/2016 |
| CONCENTRATION: | 39.6mg/ml |
| OBSERVED MW: | 47kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | SEC |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 8 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 97 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMS NSSQTSQEVY DITXXNHPTS TESKVALITG ITGQDGSYLA EFLLEKGYMV YGIIRRSSSF NTGRVEHLYK DIHITKAKFK LLYGDLTDTG NLISIIAKIK PDEIYNLAAQ SHVKVSFEMP EYTANVDGIG TLRLLEAIRA CGLEKKTKFY QASTSELYGL VQEVPQKETT PFYPRSPYAC AKLYSYWIVV NYREAYNMFA LNGILFNHES IRRGPTFVTR KITMAVARIK LGLQDCLYLG NLDAERDWGH AKDYVEAMWL MLQQEQPRDF CVATGEKHSV REFVEKAFAC IGQTVEWKGE RGTVEEHGVV DGVVRVRVDP RYFRPTEVDQ LLGDPTLAET VLGWKRKVSF EELVRGMVEG DIELLQSGDP
Validated NT Sequence
tgaggatctc cagattgcaa caattcaata tctccttcca ccattcctcg gaccaattct tcgaaactga cctttctctt ccaacccaaa actgtctcag ccaaagttgg gtctcccaat aattgatcga cctcagttgg acggaaataa cgaggatcga ctcgaactct tacaacacca tcaacaacac cgtgctcttc aacagttcct ctttctcctt tccattccac ggtttggcca atgcatgcaa aggccttttc aacaaattct ctgacgctat gtttttcacc agttgccacg caaaaatcac gtggttgctc ttgctgtaac atgagccaca tggcttcaac ataatcctta gcatgacccc aatctctttc agcatccaag ttacccaaat acaaacaatc ttgcaaacca agttttatac gggcaacagc catggtgatc tttctggtca caaatgttgg accacgacga atcgattcgt ggttgaacaa aataccattc aatgcaaaca tgttgtaagc ttctctatag ttaacaacaa tccaatagct gtacaatttg gcacaggcat atggtgaacg aggatagaat ggtgttgttt ccttttgagg aacctcttgc actagaccgt acaattcact tgtactggct tgataaaatt tggttttctt ttccaatcca caggcacgaa ttgcttccaa caaacgtaaa gtaccaatac catcaacatt agcagtatat tcaggcattt caaaactgac tttcacatga gattgagcag ccaaattgta aatttcatct ggcttaattt tggcaatgat ggaaattaag tttccggtat cggtcaagtc tccatataaa agtttgaatt ttgctttagt aatatgaata tccttgtaca agtgttcaac tcttccagtg ttgaaggatg aagaacgtct aatgatacca tacaccatat aacccttttc caacaaaaat tcagcaagat aggaaccatc ttgtcctgta atacctgtaa tcaaggccac cttggattcg gtgctggttg ggtgattcan nnnggtaatg tcatagactt cttgcgaggt ttgtgatgaa ttgctcatat ggtggtggtg gtggtgagcc at
Expressed Protein Sequence
MAHHHHHHMS NSSQTSQEVY DITQVNHPTS TESKVALITG ITGQDGSYLA EFLLEKGYMV YGIIRRSSSF NTGRVEHLYK DIHITKAKFK LLYGDLTDTG NLISIIAKIK PDEIYNLAAQ SHVKVSFEMP EYTANVDGIG TLRLLEAIRA CGLEKKTKFY QASTSELYGL VQEVPQKETT PFYPRSPYAC AKLYSYWIVV NYREAYNMFA LNGILFNHES IRRGPTFVTR KITMAVARIK LGLQDCLYLG NLDAERDWGH AKDYVEAMWL MLQQEQPRDF CVATGEKHSV REFVEKAFAC IGQTVEWKGE RGTVEEHGVV DGVVRVRVDP RYFRPTEVDQ LLGDPTLAET VLGWKRKVSF EELVRGMVEG DIELLQSGDP QVVQQQYH
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAGCA ATTCATCACA AACCTCGCAA GAAGTCTATG ACATTACCCA AGTGAATCAC CCAACCAGCA CCGAATCCAA GGTGGCCTTG ATTACAGGTA TTACAGGACA AGATGGTTCC TATCTTGCTG AATTTTTGTT GGAAAAGGGT TATATGGTGT ATGGTATCAT TAGACGTTCT TCATCCTTCA ACACTGGAAG AGTTGAACAC TTGTACAAGG ATATTCATAT TACTAAAGCA AAATTCAAAC TTTTATATGG AGACTTGACC GATACCGGAA ACTTAATTTC CATCATTGCC AAAATTAAGC CAGATGAAAT TTACAATTTG GCTGCTCAAT CTCATGTGAA AGTCAGTTTT GAAATGCCTG AATATACTGC TAATGTTGAT GGTATTGGTA CTTTACGTTT GTTGGAAGCA ATTCGTGCCT GTGGATTGGA AAAGAAAACC AAATTTTATC AAGCCAGTAC AAGTGAATTG TACGGTCTAG TGCAAGAGGT TCCTCAAAAG GAAACAACAC CATTCTATCC TCGTTCACCA TATGCCTGTG CCAAATTGTA CAGCTATTGG ATTGTTGTTA ACTATAGAGA AGCTTACAAC ATGTTTGCAT TGAATGGTAT TTTGTTCAAC CACGAATCGA TTCGTCGTGG TCCAACATTT GTGACCAGAA AGATCACCAT GGCTGTTGCC CGTATAAAAC TTGGTTTGCA AGATTGTTTG TATTTGGGTA ACTTGGATGC TGAAAGAGAT TGGGGTCATG CTAAGGATTA TGTTGAAGCC ATGTGGCTCA TGTTACAGCA AGAGCAACCA CGTGATTTTT GCGTGGCAAC TGGTGAAAAA CATAGCGTCA GAGAATTTGT TGAAAAGGCC TTTGCATGCA TTGGCCAAAC CGTGGAATGG AAAGGAGAAA GAGGAACTGT TGAAGAGCAC GGTGTTGTTG ATGGTGTTGT AAGAGTTCGA GTCGATCCTC GTTATTTCCG TCCAACTGAG GTCGATCAAT TATTGGGAGA CCCAACTTTG GCTGAGACAG TTTTGGGTTG GAAGAGAAAG GTCAGTTTCG AAGAATTGGT CCGAGGAATG GTGGAAGGAG ATATTGAATT GTTGCAATCT GGAGATCCTC AAGTGGTTCA ACAACAATAT CATTGAGTAA GATAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAAT TCTTGAAGAC GAAAGGGCCT CGTGATACGC CTATTTTTAT AGGTTAATGT CATGATAATA ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT GTTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GCAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC TATGAGAAAG CGCCACGCTT CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCTGATGC GGTATTTTCT CCTTACGCAT CTGTGCGGTA TTTCACACCG CATATATGGT GCACTCTCAG TACAATCTGC TCTGATGCCG CATAGTTAAG CCAGTATACA CTCCGCTATC GCTACGTGAC TGGGTCATGG CTGCGCCCCG ACACCCGCCA ACACCCGCTG ACGCGCCCTG ACGGGCTTGT CTGCTCCCGG CATCCGCTTA CAGACAAGCT GTGACCGTCT CCGGGAGCTG CATGTGTCAG AGGTTTTCAC CGTCATCACC GAAACGCGCG AGGCAGCTGC GGTAAAGCTC ATCAGCGTGG TCGTGAAGCG ATTCACAGAT GTCTGCCTGT TCATCCGCGT CCAGCTCGTT GAGTTTCTCC AGAAGCGTTA ATGTCTGGCT TCTGATAAAG CGGGCCATGT TAAGGGCGGT TTTTTCCTGT TTGGTCACTG ATGCCTCCGT GTAAGGGGGA TTTCTGTTCA TGGGGGTAAT GATACCGATG AAACGAGAGA GGATGCTCAC GATACGGGTT ACTGATGATG AACATGCCCG GTTACTGGAA CGTTGTGAGG GTAAACAACT GGCGGTATGG ATGCGGCGGG ACCAGAGAAA AATCACTCAG GGTCAATGCC AGCGCTTCGT TAATACAGAT GTAGGTGTTC CACAGGGTAG CCAGCAGCAT CCTGCGATGC AGATCCGGAA CATAATGGTG CAGGGCGCTG ACTTCCGCGT TTCCAGACTT TACGAAACAC GGAAACCGAA GACCATTCAT GTTGTTGCTC AGGTCGCAGA CGTTTTGCAG CAGCAGTCGC TTCACGTTCG CTCGCGTATC GGTGATTCAT TCTGCTAACC AGTAAGGCAA CCCCGCCAGC CTAGCCGGGT CCTCAACGAC AGGAGCACGA TCATGCGCAC CCGTGGCCAG GACCCAACGC TGCCCGAGAT GCGCCGCGTG CGGCTGCTGG AGATGGCGGA CGCGATGGAT ATGTTCTGCC AAGGGTTGGT TTGCGCATTC ACAGTTCTCC GCAAGAATTG ATTGGCTCCA ATTCTTGGAG TGGTGAATCC GTTAGCGAGG TGCCGCCGGC TTCCATTCAG GTCGAGGTGG CCCGGCTCCA TGCACCGCGA CGCAACGCGG GGAGGCAGAC AAGGTATAGG GCGGCGCCTA CAATCCATGC CAACCCGTTC CATGTGCTCG CCGAGGCGGC ATAAATCGCC GTGACGATCA GCGGTCCAGT GATCGAAGTT AGGCTGGTAA GAGCCGCGAG CGATCCTTGA AGCTGTCCCT GATGGTCGTC ATCTACCTGC CTGGACAGCA TGGCCTGCAA CGCGGGCATC CCGATGCCGC CGGAAGCGAG AAGAATCATA ATGGGGAAGG CCATCCAGCC TCGCGTCGCG AACGCCAGCA AGACGTAGCC CAGCGCGTCG GCCGCCATGC CGGCGATAAT GGCCTGCTTC TCGCCGAAAC GTTTGGTGGC GGGACCAGTG ACGAAGGCTT GAGCGAGGGC GTGCAAGATT CCGAATACCG CAAGCGACAG GCCGATCATC GTCGCGCTCC AGCGAAAGCG GTCCTCGCCG AAAATGACCC AGAGCGCTGC CGGCACCTGT CCTACGAGTT GCATGATAAA GAAGACAGTC ATAAGTGCGG CGACGATAGT CATGCCCCGC GCCCACCGGA AGGAGCTGAC TGGGTTGAAG GCTCTCAAGG GCATCGGTCG ACGCTCTCCC TTATGCGACT CCTGCATTAG GAAGCAGCCC AGTAGTAGGT TGAGGCCGTT GAGCACCGCC GCCGCAAGGA ATGGTGCATG CAAGGAGATG GCGCCCAACA GTCCCCCGGC CACGGGGCCT GCCACCATAC CCACGCCGAA ACAAGCGCTC ATGAGCCCGA AGTGGCGAGC CCGATCTTCC CCATCGGTGA TGTCGGCGAT ATAGGCGCCA GCAACCGCAC CTGTGGCGCC GGTGATGCCG GCCACGATGC GTCCGGCGTA GAGGATCGAG ATCTCGATCC CGCGAAAT