NafoA.00085.a
gdp-l-fucose synthetase
| CENTER ID: | NafoA.00085.a |
| ORGANISM: | Naegleria fowleri ATCC 30863 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| NafoA.00085.a.B1.GE40141 | Full length(NafoA.00085.a) | 1 | 333 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| NafoA.00085.a.B1.PS38274 | Full length(NafoA.00085.a) | 1 | 333 |
Structures
6AQY
DEPOSITED: 8/21/2017
DETERMINATION: XRay
CLONE: NafoA.00085.a.B1.GE40141
PROTEIN: NafoA.00085.a.B1.PS38274
6AQZ
DEPOSITED: 8/21/2017
DETERMINATION: XRay
CLONE: NafoA.00085.a.B1.GE40141
PROTEIN: NafoA.00085.a.B1.PS38274
Publications by SSGCID
Naegleria fowleri: Protein structures to facilitate drug discovery for the deadly, pathogenic free-living amoeba
Abendroth J, Barrett LK, Dranow DM, Edwards TE, Myler PJ, Phan IQ, Staker B, Subramanian S, Tran N, Van Voorhis WC, Craig JC, Smith C, Chun IKH, Kyle D, Barrett KF, Morris JC, Tillery L, Rice CA, Goldstein J, Lassner JW, Xu L, Young RM, Osterhout B, Leahy JW, Manetsch R
PLoS ONE - 2021
volume 16, issue 3, pages e0241738
PMID: 33760815; PMCID: PMC7990177
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| EuPathDB: | AmoebaDB:NF0109030 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAQREVSDQP ITLTQDDVIL VTGGTGLFGK AVEHIVKKEQ IKGKWVFLGS KDGDLRDADA CKQLFEKYRP TYVIHLAAFV GGLFKNMNFK VSFWLDNVNM NNNILTCCYD FGVKKTISCL STCVFPDKIE YPITEEKLHE GPPHFSNNAY AYAKRMLDML GRWYNEKAVN EGKSCLFTSV IPTNLFGPHD YFNVEAGHVL PGLMHKCYKA QQNGTDFVVF GSGKPLRQFL YSHDAARMLL WTMFNYQSEE PIMLCVSEED EKSIGQVAQT IKDAFNFTGN MVFDTSKADG QYKKTSSNAK FLRLNPTFQY TPFEQAIKET VQWFLENYET ARK
NT Sequence
ATGGCTCAAC GAGAAGTCTC TGATCAGCCC ATCACACTCA CTCAGGATGA TGTAATTTTG GTTACGGGAG GAACTGGTTT GTTTGGAAAG GCAGTCGAAC ATATTGTAAA GAAGGAACAA ATTAAAGGAA AATGGGTCTT CTTGGGAAGC AAGGATGGTG ATCTCCGTGA TGCCGATGCC TGCAAACAAC TTTTTGAAAA GTACAGACCA ACCTATGTGA TTCATTTAGC TGCTTTTGTT GGAGGTCTTT TCAAGAATAT GAATTTTAAA GTGAGCTTTT GGTTGGATAA CGTGAATATG AATAATAATA TTTTGACTTG TTGTTATGAT TTCGGAGTGA AAAAGACAAT ATCATGTCTC TCTACTTGTG TTTTTCCAGA CAAGATTGAA TATCCAATTA CTGAAGAAAA ACTTCATGAA GGACCTCCAC ACTTTAGTAA TAATGCCTAT GCCTACGCCA AAAGAATGCT CGACATGTTA GGAAGATGGT ACAATGAAAA GGCCGTCAAT GAAGGAAAAT CATGTCTCTT TACAAGTGTC ATTCCAACCA ATTTGTTTGG ACCACACGAT TATTTTAACG TAGAGGCTGG ACACGTCTTG CCTGGACTCA TGCACAAGTG CTATAAGGCT CAACAAAATG GCACTGATTT TGTTGTGTTT GGCTCAGGAA AGCCATTGAG ACAATTTTTG TATTCTCACG ATGCTGCACG TATGTTGTTG TGGACCATGT TCAATTATCA ATCCGAAGAA CCAATCATGT TGTGTGTGAG TGAGGAGGAT GAAAAGAGCA TTGGACAAGT CGCTCAAACT ATTAAGGATG CTTTCAACTT CACTGGCAAC ATGGTTTTCG ATACCTCAAA GGCAGATGGC CAATACAAGA AGACTTCAAG TAATGCCAAG TTCTTGCGTT TAAATCCAAC GTTCCAATAT ACGCCATTCG AACAAGCCAT CAAGGAAACC GTGCAATGGT TCTTGGAAAA TTATGAAACT GCCAGAAAG
Details for NafoA.00085.a.B1.GE40141
| HARVESTED ON: | 4/6/2016 |
| SEQUENCED ON: | 4/13/2016 |
| EXPECTED MW: | 39kDa |
| OBSERVED MW: | 43kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Many (50-100) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 97 |
| PERCENT COVERAGE: | 100 |
Validated AA Sequence
MAHHHHHHMA QREVSDQPIT LXXXDVILVT GGTGLFGKAV EHIVKKEQIK GKWVFLGSKD GDLRDADACK QPFEKYRPTY VIHLAAFVGG LFKNMNFKVS FWLDNVNMNN NILTCCYDFG VKKTISCLST CVFPDKIEYP ITEEKLHEGP PHFSNNAYAY AKRMLDMLGR WYNEKAVNEG KSCLFTSVIP TNLFGPHDNF NVEAGHVLPG LMHKCYKAQQ NGTDFVVFGS GKPLRQFLYS HDAARMLLWT MFNYQSEEPI MLCVSEEDEK SIGQVAQTIK DAFNFTGNMV FDTSKADGQY KKTSSNAKFL RLNPTFQYTP FEQAIKETVQ WFLXXXETAR K
Validated NT Sequence
ttactcactt actttctggc agtttcatnn nntnccaaga accattgcac ggtttccttg atggcttgtt cgaatggcgt atattggaac gttggattta aacgcaagaa cttggcatta cttgaagtct tcttgtattg gccatctgcc tttgaggtat cgaaaaccat gttgccagtg aagttgaaag catccttaat agtttgagcg acttgtccaa tgctcttttc atcctcctca ctcacacaca acatgattgg ttcttcggat tgataattga acatggtcca caacaacata cgtgcagcat cgtgagaata caaaaattgt ctcaatggct ttcctgagcc aaacacaaca aaatcagtgc cattttgttg agccttatag cacttgtgca tgagtccagg caagacgtgt ccagcctcta cgttaaagtt atcgtgtggt ccaaacaaat tggttggaat gacacttgta aagagacatg attttccttc attgacggcc ttttcattgt accatcttcc taacatgtcg agcattcttt tggcgtaggc ataggcatta ttactaaagt gtggaggtcc ttcatgaagt ttttcttcag taattggata ttcaatcttg tctggaaaaa cacaagtaga gagacatgat attgtctttt tcactccgaa atcataacaa caagtcaaaa tattattatt catattcacg ttatccaacc aaaagctcac tttaaaattc atattcttga aaagacctcc aacaaaagca gctaaatgaa tcacataggt tggtctgtac ttttcaaaag gttgtttgca ggcatcggca tcacggagat caccatcctt gcttcccaag aagacccatt ttcctttaat ttgttccttc tttacaatat gttcgactgc ctttccaaac aaaccagttc ctcccgtaac caaaattaca tcannnnnnn tgagtgtgat gggctgatca gagacttctc gttgagccat atggtggtgg tggtggtgag ccat
Expected Protein Sequence
MAHHHHHHMA QREVSDQPIT LTQDDVILVT GGTGLFGKAV EHIVKKEQIK GKWVFLGSKD GDLRDADACK QLFEKYRPTY VIHLAAFVGG LFKNMNFKVS FWLDNVNMNN NILTCCYDFG VKKTISCLST CVFPDKIEYP ITEEKLHEGP PHFSNNAYAY AKRMLDMLGR WYNEKAVNEG KSCLFTSVIP TNLFGPHDYF NVEAGHVLPG LMHKCYKAQQ NGTDFVVFGS GKPLRQFLYS HDAARMLLWT MFNYQSEEPI MLCVSEEDEK SIGQVAQTIK DAFNFTGNMV FDTSKADGQY KKTSSNAKFL RLNPTFQYTP FEQAIKETVQ WFLENYETAR K
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatggctc aacgagaagt ctctgatcag cccatcacac tcactcagga tgatgtaatt ttggttacgg gaggaactgg tttgtttgga aaggcagtcg aacatattgt aaagaaggaa caaattaaag gaaaatgggt cttcttggga agcaaggatg gtgatctccg tgatgccgat gcctgcaaac aactttttga aaagtacaga ccaacctatg tgattcattt agctgctttt gttggaggtc ttttcaagaa tatgaatttt aaagtgagct tttggttgga taacgtgaat atgaataata atattttgac ttgttgttat gatttcggag tgaaaaagac aatatcatgt ctctctactt gtgtttttcc agacaagatt gaatatccaa ttactgaaga aaaacttcat gaaggacctc cacactttag taataatgcc tatgcctacg ccaaaagaat gctcgacatg ttaggaagat ggtacaatga aaaggccgtc aatgaaggaa aatcatgtct ctttacaagt gtcattccaa ccaatttgtt tggaccacac gattatttta acgtagaggc tggacacgtc ttgcctggac tcatgcacaa gtgctataag gctcaacaaa atggcactga ttttgttgtg tttggctcag gaaagccatt gagacaattt ttgtattctc acgatgctgc acgtatgttg ttgtggacca tgttcaatta tcaatccgaa gaaccaatca tgttgtgtgt gagtgaggag gatgaaaaga gcattggaca agtcgctcaa actattaagg atgctttcaa cttcactggc aacatggttt tcgatacctc aaaggcagat ggccaataca agaagacttc aagtaatgcc aagttcttgc gtttaaatcc aacgttccaa tatacgccat tcgaacaagc catcaaggaa accgtgcaat ggttcttgga aaattatgaa actgccagaa agtgagtaag ataggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga cgctctccct tatgcgactc ctgcattagg aagcagccca gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga tctcgatccc gcgaaat
Details for NafoA.00085.a.B1.PS38274
| PURIFICATION DATe: | 5/10/2017 |
| CONCENTRATION: | 12mg/ml |
| OBSERVED MW: | 32kDa |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | SEC Buffer: 20mM HEPES pH 7.0, 300mM NaCl,5% glcerol, 1mM TCEP |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 12 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 97 |
| PERCENT COVERAGE: | 100 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMA QREVSDQPIT LXXXDVILVT GGTGLFGKAV EHIVKKEQIK GKWVFLGSKD GDLRDADACK QPFEKYRPTY VIHLAAFVGG LFKNMNFKVS FWLDNVNMNN NILTCCYDFG VKKTISCLST CVFPDKIEYP ITEEKLHEGP PHFSNNAYAY AKRMLDMLGR WYNEKAVNEG KSCLFTSVIP TNLFGPHDNF NVEAGHVLPG LMHKCYKAQQ NGTDFVVFGS GKPLRQFLYS HDAARMLLWT MFNYQSEEPI MLCVSEEDEK SIGQVAQTIK DAFNFTGNMV FDTSKADGQY KKTSSNAKFL RLNPTFQYTP FEQAIKETVQ WFLXXXETAR K
Validated NT Sequence
ttactcactt actttctggc agtttcatnn nntnccaaga accattgcac ggtttccttg atggcttgtt cgaatggcgt atattggaac gttggattta aacgcaagaa cttggcatta cttgaagtct tcttgtattg gccatctgcc tttgaggtat cgaaaaccat gttgccagtg aagttgaaag catccttaat agtttgagcg acttgtccaa tgctcttttc atcctcctca ctcacacaca acatgattgg ttcttcggat tgataattga acatggtcca caacaacata cgtgcagcat cgtgagaata caaaaattgt ctcaatggct ttcctgagcc aaacacaaca aaatcagtgc cattttgttg agccttatag cacttgtgca tgagtccagg caagacgtgt ccagcctcta cgttaaagtt atcgtgtggt ccaaacaaat tggttggaat gacacttgta aagagacatg attttccttc attgacggcc ttttcattgt accatcttcc taacatgtcg agcattcttt tggcgtaggc ataggcatta ttactaaagt gtggaggtcc ttcatgaagt ttttcttcag taattggata ttcaatcttg tctggaaaaa cacaagtaga gagacatgat attgtctttt tcactccgaa atcataacaa caagtcaaaa tattattatt catattcacg ttatccaacc aaaagctcac tttaaaattc atattcttga aaagacctcc aacaaaagca gctaaatgaa tcacataggt tggtctgtac ttttcaaaag gttgtttgca ggcatcggca tcacggagat caccatcctt gcttcccaag aagacccatt ttcctttaat ttgttccttc tttacaatat gttcgactgc ctttccaaac aaaccagttc ctcccgtaac caaaattaca tcannnnnnn tgagtgtgat gggctgatca gagacttctc gttgagccat atggtggtgg tggtggtgag ccat
Expressed Protein Sequence
MAHHHHHHMA QREVSDQPIT LTQDDVILVT GGTGLFGKAV EHIVKKEQIK GKWVFLGSKD GDLRDADACK QLFEKYRPTY VIHLAAFVGG LFKNMNFKVS FWLDNVNMNN NILTCCYDFG VKKTISCLST CVFPDKIEYP ITEEKLHEGP PHFSNNAYAY AKRMLDMLGR WYNEKAVNEG KSCLFTSVIP TNLFGPHDYF NVEAGHVLPG LMHKCYKAQQ NGTDFVVFGS GKPLRQFLYS HDAARMLLWT MFNYQSEEPI MLCVSEEDEK SIGQVAQTIK DAFNFTGNMV FDTSKADGQY KKTSSNAKFL RLNPTFQYTP FEQAIKETVQ WFLENYETAR K
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGGCTC AACGAGAAGT CTCTGATCAG CCCATCACAC TCACTCAGGA TGATGTAATT TTGGTTACGG GAGGAACTGG TTTGTTTGGA AAGGCAGTCG AACATATTGT AAAGAAGGAA CAAATTAAAG GAAAATGGGT CTTCTTGGGA AGCAAGGATG GTGATCTCCG TGATGCCGAT GCCTGCAAAC AACTTTTTGA AAAGTACAGA CCAACCTATG TGATTCATTT AGCTGCTTTT GTTGGAGGTC TTTTCAAGAA TATGAATTTT AAAGTGAGCT TTTGGTTGGA TAACGTGAAT ATGAATAATA ATATTTTGAC TTGTTGTTAT GATTTCGGAG TGAAAAAGAC AATATCATGT CTCTCTACTT GTGTTTTTCC AGACAAGATT GAATATCCAA TTACTGAAGA AAAACTTCAT GAAGGACCTC CACACTTTAG TAATAATGCC TATGCCTACG CCAAAAGAAT GCTCGACATG TTAGGAAGAT GGTACAATGA AAAGGCCGTC AATGAAGGAA AATCATGTCT CTTTACAAGT GTCATTCCAA CCAATTTGTT TGGACCACAC GATTATTTTA ACGTAGAGGC TGGACACGTC TTGCCTGGAC TCATGCACAA GTGCTATAAG GCTCAACAAA ATGGCACTGA TTTTGTTGTG TTTGGCTCAG GAAAGCCATT GAGACAATTT TTGTATTCTC ACGATGCTGC ACGTATGTTG TTGTGGACCA TGTTCAATTA TCAATCCGAA GAACCAATCA TGTTGTGTGT GAGTGAGGAG GATGAAAAGA GCATTGGACA AGTCGCTCAA ACTATTAAGG ATGCTTTCAA CTTCACTGGC AACATGGTTT TCGATACCTC AAAGGCAGAT GGCCAATACA AGAAGACTTC AAGTAATGCC AAGTTCTTGC GTTTAAATCC AACGTTCCAA TATACGCCAT TCGAACAAGC CATCAAGGAA ACCGTGCAAT GGTTCTTGGA AAATTATGAA ACTGCCAGAA AGTGAGTAAG ATAGGATCCG GCTGCTAACA AAGCCCGAAA GGAAGCTGAG TTGGCTGCTG CCACCGCTGA GCAATAACTA GCATAACCCC TTGGGGCCTC TAAACGGGTC TTGAGGGGTT TTTTGCTGAA AGGAGGAACT ATATCCGGAT ATCCACAGGA CGGGTGTGGT CGCCATGATC GCGTAGTCGA TAGTGGCTCC AAGTAGCGAA GCGAGCAGGA CTGGGCGGCG GCCAAAGCGG TCGGACAGTG CTCCGAGAAC GGGTGCGCAT AGAAATTGCA TCAACGCATA TAGCGCTAGC AGCACGCCAT AGTGACTGGC GATGCTGTCG GAATGGACGA TATCCCGCAA GAGGCCCGGC AGTACCGGCA TAACCAAGCC TATGCCTACA GCATCCAGGG TGACGGTGCC GAGGATGACG ATGAGCGCAT TGTTAGATTT CATACACGGT GCCTGACTGC GTTAGCAATT TAACTGTGAT AAACTACCGC ATTAAAGCTT ATCGATGATA AGCTGTCAAA CATGAGAATT CTTGAAGACG AAAGGGCCTC GTGATACGCC TATTTTTATA GGTTAATGTC ATGATAATAA TGGTTTCTTA GACGTCAGGT GGCACTTTTC GGGGAAATGT GCGCGGAACC CCTATTTGTT TATTTTTCTA AATACATTCA AATATGTATC CGCTCATGAG ACAATAACCC TGATAAATGC TTCAATAATA TTGAAAAAGG AAGAGTATGA GTATTCAACA TTTCCGTGTC GCCCTTATTC CCTTTTTTGC GGCATTTTGC CTTCCTGTTT TTGCTCACCC AGAAACGCTG GTGAAAGTAA AAGATGCTGA AGATCAGTTG GGTGCACGAG TGGGTTACAT CGAACTGGAT CTCAACAGCG GTAAGATCCT TGAGAGTTTT CGCCCCGAAG AACGTTTTCC AATGATGAGC ACTTTTAAAG TTCTGCTATG TGGCGCGGTA TTATCCCGTG TTGACGCCGG GCAAGAGCAA CTCGGTCGCC GCATACACTA TTCTCAGAAT GACTTGGTTG AGTACTCACC AGTCACAGAA AAGCATCTTA CGGATGGCAT GACAGTAAGA GAATTATGCA GTGCTGCCAT AACCATGAGT GATAACACTG CGGCCAACTT ACTTCTGACA ACGATCGGAG GACCGAAGGA GCTAACCGCT TTTTTGCACA ACATGGGGGA TCATGTAACT CGCCTTGATC GTTGGGAACC GGAGCTGAAT GAAGCCATAC CAAACGACGA GCGTGACACC ACGATGCCTG CAGCAATGGC AACAACGTTG CGCAAACTAT TAACTGGCGA ACTACTTACT CTAGCTTCCC GGCAACAATT AATAGACTGG ATGGAGGCGG ATAAAGTTGC AGGACCACTT CTGCGCTCGG CCCTTCCGGC TGGCTGGTTT ATTGCTGATA AATCTGGAGC CGGTGAGCGT GGGTCTCGCG GTATCATTGC AGCACTGGGG CCAGATGGTA AGCCCTCCCG TATCGTAGTT ATCTACACGA CGGGGAGTCA GGCAACTATG GATGAACGAA ATAGACAGAT CGCTGAGATA GGTGCCTCAC TGATTAAGCA TTGGTAACTG TCAGACCAAG TTTACTCATA TATACTTTAG ATTGATTTAA AACTTCATTT TTAATTTAAA AGGATCTAGG TGAAGATCCT TTTTGATAAT CTCATGACCA AAATCCCTTA ACGTGAGTTT TCGTTCCACT GAGCGTCAGA CCCCGTAGAA AAGATCAAAG GATCTTCTTG AGATCCTTTT TTTCTGCGCG TAATCTGCTG CTTGCAAACA AAAAAACCAC CGCTACCAGC GGTGGTTTGT TTGCCGGATC AAGAGCTACC AACTCTTTTT CCGAAGGTAA CTGGCTTCAG CAGAGCGCAG ATACCAAATA CTGTCCTTCT AGTGTAGCCG TAGTTAGGCC ACCACTTCAA GAACTCTGTA GCACCGCCTA CATACCTCGC TCTGCTAATC CTGTTACCAG TGGCTGCTGC CAGTGGCGAT AAGTCGTGTC TTACCGGGTT GGACTCAAGA CGATAGTTAC CGGATAAGGC GCAGCGGTCG GGCTGAACGG GGGGTTCGTG CACACAGCCC AGCTTGGAGC GAACGACCTA CACCGAACTG AGATACCTAC AGCGTGAGCT ATGAGAAAGC GCCACGCTTC CCGAAGGGAG AAAGGCGGAC AGGTATCCGG TAAGCGGCAG GGTCGGAACA GGAGAGCGCA CGAGGGAGCT TCCAGGGGGA AACGCCTGGT ATCTTTATAG TCCTGTCGGG TTTCGCCACC TCTGACTTGA GCGTCGATTT TTGTGATGCT CGTCAGGGGG GCGGAGCCTA TGGAAAAACG CCAGCAACGC GGCCTTTTTA CGGTTCCTGG CCTTTTGCTG GCCTTTTGCT CACATGTTCT TTCCTGCGTT ATCCCCTGAT TCTGTGGATA ACCGTATTAC CGCCTTTGAG TGAGCTGATA CCGCTCGCCG CAGCCGAACG ACCGAGCGCA GCGAGTCAGT GAGCGAGGAA GCGGAAGAGC GCCTGATGCG GTATTTTCTC CTTACGCATC TGTGCGGTAT TTCACACCGC ATATATGGTG CACTCTCAGT ACAATCTGCT CTGATGCCGC ATAGTTAAGC CAGTATACAC TCCGCTATCG CTACGTGACT GGGTCATGGC TGCGCCCCGA CACCCGCCAA CACCCGCTGA CGCGCCCTGA CGGGCTTGTC TGCTCCCGGC ATCCGCTTAC AGACAAGCTG TGACCGTCTC CGGGAGCTGC ATGTGTCAGA GGTTTTCACC GTCATCACCG AAACGCGCGA GGCAGCTGCG GTAAAGCTCA TCAGCGTGGT CGTGAAGCGA TTCACAGATG TCTGCCTGTT CATCCGCGTC CAGCTCGTTG AGTTTCTCCA GAAGCGTTAA TGTCTGGCTT CTGATAAAGC GGGCCATGTT AAGGGCGGTT TTTTCCTGTT TGGTCACTGA TGCCTCCGTG TAAGGGGGAT TTCTGTTCAT GGGGGTAATG ATACCGATGA AACGAGAGAG GATGCTCACG ATACGGGTTA CTGATGATGA ACATGCCCGG TTACTGGAAC GTTGTGAGGG TAAACAACTG GCGGTATGGA TGCGGCGGGA CCAGAGAAAA ATCACTCAGG GTCAATGCCA GCGCTTCGTT AATACAGATG TAGGTGTTCC ACAGGGTAGC CAGCAGCATC CTGCGATGCA GATCCGGAAC ATAATGGTGC AGGGCGCTGA CTTCCGCGTT TCCAGACTTT ACGAAACACG GAAACCGAAG ACCATTCATG TTGTTGCTCA GGTCGCAGAC GTTTTGCAGC AGCAGTCGCT TCACGTTCGC TCGCGTATCG GTGATTCATT CTGCTAACCA GTAAGGCAAC CCCGCCAGCC TAGCCGGGTC CTCAACGACA GGAGCACGAT CATGCGCACC CGTGGCCAGG ACCCAACGCT GCCCGAGATG CGCCGCGTGC GGCTGCTGGA GATGGCGGAC GCGATGGATA TGTTCTGCCA AGGGTTGGTT TGCGCATTCA CAGTTCTCCG CAAGAATTGA TTGGCTCCAA TTCTTGGAGT GGTGAATCCG TTAGCGAGGT GCCGCCGGCT TCCATTCAGG TCGAGGTGGC CCGGCTCCAT GCACCGCGAC GCAACGCGGG GAGGCAGACA AGGTATAGGG CGGCGCCTAC AATCCATGCC AACCCGTTCC ATGTGCTCGC CGAGGCGGCA TAAATCGCCG TGACGATCAG CGGTCCAGTG ATCGAAGTTA GGCTGGTAAG AGCCGCGAGC GATCCTTGAA GCTGTCCCTG ATGGTCGTCA TCTACCTGCC TGGACAGCAT GGCCTGCAAC GCGGGCATCC CGATGCCGCC GGAAGCGAGA AGAATCATAA TGGGGAAGGC CATCCAGCCT CGCGTCGCGA ACGCCAGCAA GACGTAGCCC AGCGCGTCGG CCGCCATGCC GGCGATAATG GCCTGCTTCT CGCCGAAACG TTTGGTGGCG GGACCAGTGA CGAAGGCTTG AGCGAGGGCG TGCAAGATTC CGAATACCGC AAGCGACAGG CCGATCATCG TCGCGCTCCA GCGAAAGCGG TCCTCGCCGA AAATGACCCA GAGCGCTGCC GGCACCTGTC CTACGAGTTG CATGATAAAG AAGACAGTCA TAAGTGCGGC GACGATAGTC ATGCCCCGCG CCCACCGGAA GGAGCTGACT GGGTTGAAGG CTCTCAAGGG CATCGGTCGA CGCTCTCCCT TATGCGACTC CTGCATTAGG AAGCAGCCCA GTAGTAGGTT GAGGCCGTTG AGCACCGCCG CCGCAAGGAA TGGTGCATGC AAGGAGATGG CGCCCAACAG TCCCCCGGCC ACGGGGCCTG CCACCATACC CACGCCGAAA CAAGCGCTCA TGAGCCCGAA GTGGCGAGCC CGATCTTCCC CATCGGTGAT GTCGGCGATA TAGGCGCCAG CAACCGCACC TGTGGCGCCG GTGATGCCGG CCACGATGCG TCCGGCGTAG AGGATCGAGA TCTCGATCCC GCGAAAT