NafoA.00032.a
s-adenosyl-l-homocysteine hydrolase
| CENTER ID: | NafoA.00032.a |
| ORGANISM: | Naegleria fowleri ATCC 30863 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| NafoA.00032.a.B1.GE40139 | Full length(NafoA.00032.a) | 1 | 472 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| NafoA.00032.a.B1.PW37932 | Full length(NafoA.00032.a) | 1 | 472 | ||
| NafoA.00032.a.B1.PW38615 | Full length(NafoA.00032.a) | 1 | 472 |
Structures
5V96
DEPOSITED: 3/23/2017
DETERMINATION: XRay
CLONE: NafoA.00032.a.B1.GE40139
PROTEIN: NafoA.00032.a.B1.PW37932
Publications by SSGCID
Naegleria fowleri: Protein structures to facilitate drug discovery for the deadly, pathogenic free-living amoeba
Abendroth J, Barrett LK, Dranow DM, Edwards TE, Myler PJ, Phan IQ, Staker B, Subramanian S, Tran N, Van Voorhis WC, Craig JC, Smith C, Chun IKH, Kyle D, Barrett KF, Morris JC, Tillery L, Rice CA, Goldstein J, Lassner JW, Xu L, Young RM, Osterhout B, Leahy JW, Manetsch R
PLoS ONE - 2021
volume 16, issue 3, pages e0241738
PMID: 33760815; PMCID: PMC7990177
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| EuPathDB: | AmoebaDB:NF0035640 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSQTVTTTIE YQVKDMSLAS LGRKRIEMAE KEMPGLMACR AKYGEEKPLN GVRITGSLHM TVETAVLIET LKAIGGNIRW CSCNIFSTQD DAAAAIAAAN TPVFAWKGET LVEYWECTWK AIRFGPYQGP QLIVDDGGDA TLLIHRGFQA EKEPSILDED GGVEELRIVN NLLKRILKEE PGFFSKIVPD IKGVSEETTT GVHRLYAMQK QGTLLFPAIN VNDSVTKSKF DNIYGCRHSL LDGLNRATDV MLAGKECVIC GFGDVGKGCA EALKGQGARV IVTEIDPINA LQACMAGYTV KTVEDCLATA DIYVTATGNK DIITLDHMKK MKDMAIICNI GHFDNEIDVL GLKTCEGVKE INIKPQVDQF LFPDGHSIIL LAQGRLVNLG CATGHPAFVM SASFTNQTLA QISLWKDKYE IGVYTLPKVL DEEVARLHLE KLGAKLTKLT TSQADYIGVN ANGPYKADHY RY
NT Sequence
ATGAGCCAAA CAGTGACCAC CACTATTGAA TACCAAGTCA AGGACATGAG CCTTGCTTCT TTGGGCCGTA AGAGAATTGA AATGGCTGAA AAGGAAATGC CTGGTTTGAT GGCTTGCAGA GCCAAGTACG GTGAAGAGAA GCCATTGAAC GGAGTCAGAA TCACCGGAAG TCTCCACATG ACCGTTGAAA CTGCTGTTCT TATTGAAACT CTCAAGGCCA TCGGCGGTAA CATCCGTTGG TGCAGTTGTA ACATCTTCTC CACTCAAGAC GATGCCGCTG CTGCCATTGC TGCTGCTAAT ACTCCAGTCT TTGCTTGGAA GGGAGAGACT TTGGTTGAAT ATTGGGAATG TACCTGGAAG GCCATTAGAT TCGGACCATA TCAAGGACCA CAATTGATTG TCGACGATGG TGGTGATGCT ACCTTGTTGA TTCACAGAGG ATTCCAAGCT GAAAAGGAAC CAAGCATTTT GGATGAAGAT GGTGGTGTTG AAGAATTGAG AATTGTCAAT AACTTGTTAA AGAGAATTTT GAAGGAAGAG CCTGGATTCT TCAGCAAGAT TGTTCCAGAT ATCAAGGGTG TCTCCGAAGA GACCACCACT GGTGTTCACA GATTGTATGC TATGCAAAAG CAAGGTACTT TGTTGTTCCC TGCTATTAAC GTGAACGACT CTGTTACTAA GAGCAAGTTC GACAACATTT ACGGATGCCG TCACTCCTTG TTGGATGGTT TGAACAGAGC TACTGATGTC ATGTTGGCTG GTAAGGAGTG TGTTATCTGT GGTTTCGGTG ATGTCGGTAA AGGATGCGCT GAAGCTTTGA AGGGACAAGG TGCCAGAGTT ATTGTTACTG AAATTGACCC AATTAACGCT CTCCAAGCTT GTATGGCTGG TTACACTGTC AAGACTGTTG AAGATTGTTT GGCTACTGCT GATATCTATG TCACTGCTAC TGGTAACAAG GATATCATTA CTCTCGATCA CATGAAGAAG ATGAAGGATA TGGCTATCAT TTGTAACATT GGTCACTTTG ACAATGAAAT TGATGTCCTC GGTTTGAAGA CCTGTGAAGG TGTTAAGGAA ATTAACATTA AGCCTCAAGT CGATCAATTC TTGTTCCCTG ATGGACACTC CATCATTTTG TTGGCTCAAG GACGTTTGGT CAATTTGGGT TGTGCTACTG GTCACCCTGC TTTCGTCATG TCTGCTTCAT TCACCAACCA AACATTGGCT CAAATCTCCT TGTGGAAGGA CAAGTATGAA ATTGGTGTCT ACACTTTGCC AAAGGTTTTG GACGAGGAAG TTGCCAGACT TCACTTGGAG AAGCTTGGTG CTAAGTTGAC CAAGTTGACA ACATCTCAAG CTGACTACAT TGGTGTCAAT GCTAACGGCC CATACAAGGC AGATCACTAC AGATAT
Details for NafoA.00032.a.B1.GE40139
| HARVESTED ON: | 4/6/2016 |
| SEQUENCED ON: | 4/13/2016 |
| EXPECTED MW: | 53kDa |
| OBSERVED MW: | 53kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Many (50-100) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | High Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 98 |
Validated AA Sequence
MAHHHHHHMS QTVTTTIEYQ VKDMSLASLG RKRIEMAEKE MPGLMACRAK YGEEKPLNGV RITGSLHMTV ETAVLIETLK AIGGNIRWCS CNIFSTQDDA AAAIAAANTP VFAWKGETLV EYWECTWKAI RFGPYQGPQL IVDDGGDATL LIHRGFQAEK EPSILDEDGG VEELRIVNNL LKRILKEEPG FFSKIVPDIK GVSEETTTGV HRLYAMQKQG TLLFPAINVN DSVTKSKFDN IYGCRHSLLD GLNRATDVML AGKECVVCGF GDVGKGCAEA LKGQGARVIV TEIDPINALQ ACMAGYTVKT VEDCLATADI YVTATGNKDI ITLDHMKKMK DMAIICNIGH FDNEIDVLGL KTCEGVKEIN IKPQVDQFLF PDGHSIILLA QGRLVNLGCA TGHPAFVMSA SFTNQTLAQI SLWKDKYEIG VYTLPKVLDE EVARLHLEKL GAKLTKLTTS QADYIGVNAN GP
Validated NT Sequence
gtatgggccg ttagcattga caccaatgta gtcagcttga gatgttgtca acttggtcaa cttagcacca agcttctcca agtgaagtct ggcaacttcc tcgtccaaaa cctttggcaa agtgtagaca ccaatttcat acttgtcctt ccacaaggag atttgagcca atgtttggtt ggtgaatgaa gcagacatga cgaaagcagg gtgaccagta gcacaaccca aattgaccaa acgtccttga gccaacaaaa tgatggagtg tccatcaggg aacaagaatt gatcgacttg aggcttaatg ttaatttcct taacaccttc acaggtcttc aaaccgagga catcaatttc attgtcaaag tgaccaatgt tacaaatgat agccatatcc ttcatcttct tcatgtgatc gagagtaatg atatccttgt taccagtagc agtgacatag atatcagcag tagccaaaca atcttcaaca gtcttgacag tgtaaccagc catacaagct tggagagcgt taattgggtc aatttcagta acaataactc tggcaccttg tcccttcaaa gcttcagcgc atcctttacc gacatcaccg aaaccacaga caacacactc cttaccagcc aacatgacat cagtagctct gttcaaacca tccaacaagg agtgacggca tccgtaaatg ttgtcgaact tgctcttagt aacagagtcg ttcacgttaa tagcagggaa caacaaagta ccttgctttt gcatagcata caatctgtga acaccagtgg tggtctcttc ggagacaccc ttgatatctg gaacaatctt gctgaagaat ccaggctctt ccttcaaaat tctctttaac aagttattga caattctcaa ttcttcaaca ccaccatctt catccaaaat gcttggttcc ttttcagctt ggaatcctct gtgaatcaac aaggtagcat caccaccatc gtcgacaatc aattgtggtc cttgatatgg tccgaatcta atggccttcc aggtacattc ccaatattca accaaagtct ctcccttcca agcaaagact ggagtattag cagcagcaat ggcagcagcg gcatcgtctt gagtggagaa gatgttacaa ctgcaccaac ggatgttacc gccgatggcc ttgagagttt caataagaac agcagtttca acggtcatgt ggagacttcc ggtgattctg actccgttca atggcttctc ttcaccgtac ttggctctgc aagccatcaa accaggcatt tccttttcag ccatttcaat tctcttacgg cccaaagaag caaggctcat gtccttgact tggtattcaa tagtggtggt cactgtttgg ctcatatggt ggtggtggtg gtgagccat
Expected Protein Sequence
MAHHHHHHMS QTVTTTIEYQ VKDMSLASLG RKRIEMAEKE MPGLMACRAK YGEEKPLNGV RITGSLHMTV ETAVLIETLK AIGGNIRWCS CNIFSTQDDA AAAIAAANTP VFAWKGETLV EYWECTWKAI RFGPYQGPQL IVDDGGDATL LIHRGFQAEK EPSILDEDGG VEELRIVNNL LKRILKEEPG FFSKIVPDIK GVSEETTTGV HRLYAMQKQG TLLFPAINVN DSVTKSKFDN IYGCRHSLLD GLNRATDVML AGKECVICGF GDVGKGCAEA LKGQGARVIV TEIDPINALQ ACMAGYTVKT VEDCLATADI YVTATGNKDI ITLDHMKKMK DMAIICNIGH FDNEIDVLGL KTCEGVKEIN IKPQVDQFLF PDGHSIILLA QGRLVNLGCA TGHPAFVMSA SFTNQTLAQI SLWKDKYEIG VYTLPKVLDE EVARLHLEKL GAKLTKLTTS QADYIGVNAN GPYKADHYRY
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgagcc aaacagtgac caccactatt gaataccaag tcaaggacat gagccttgct tctttgggcc gtaagagaat tgaaatggct gaaaaggaaa tgcctggttt gatggcttgc agagccaagt acggtgaaga gaagccattg aacggagtca gaatcaccgg aagtctccac atgaccgttg aaactgctgt tcttattgaa actctcaagg ccatcggcgg taacatccgt tggtgcagtt gtaacatctt ctccactcaa gacgatgccg ctgctgccat tgctgctgct aatactccag tctttgcttg gaagggagag actttggttg aatattggga atgtacctgg aaggccatta gattcggacc atatcaagga ccacaattga ttgtcgacga tggtggtgat gctaccttgt tgattcacag aggattccaa gctgaaaagg aaccaagcat tttggatgaa gatggtggtg ttgaagaatt gagaattgtc aataacttgt taaagagaat tttgaaggaa gagcctggat tcttcagcaa gattgttcca gatatcaagg gtgtctccga agagaccacc actggtgttc acagattgta tgctatgcaa aagcaaggta ctttgttgtt ccctgctatt aacgtgaacg actctgttac taagagcaag ttcgacaaca tttacggatg ccgtcactcc ttgttggatg gtttgaacag agctactgat gtcatgttgg ctggtaagga gtgtgttatc tgtggtttcg gtgatgtcgg taaaggatgc gctgaagctt tgaagggaca aggtgccaga gttattgtta ctgaaattga cccaattaac gctctccaag cttgtatggc tggttacact gtcaagactg ttgaagattg tttggctact gctgatatct atgtcactgc tactggtaac aaggatatca ttactctcga tcacatgaag aagatgaagg atatggctat catttgtaac attggtcact ttgacaatga aattgatgtc ctcggtttga agacctgtga aggtgttaag gaaattaaca ttaagcctca agtcgatcaa ttcttgttcc ctgatggaca ctccatcatt ttgttggctc aaggacgttt ggtcaatttg ggttgtgcta ctggtcaccc tgctttcgtc atgtctgctt cattcaccaa ccaaacattg gctcaaatct ccttgtggaa ggacaagtat gaaattggtg tctacacttt gccaaaggtt ttggacgagg aagttgccag acttcacttg gagaagcttg gtgctaagtt gaccaagttg acaacatctc aagctgacta cattggtgtc aatgctaacg gcccatacaa ggcagatcac tacagatatt gagtaagata ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg aaat
Details for NafoA.00032.a.B1.PW37932
| PURIFICATION DATe: | 7/12/2016 |
| CONCENTRATION: | 39.5mg/ml |
| OBSERVED MW: | 49kDa |
| EXPRESSION LEVEL: | Low Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, and 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 4 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 98 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMS QTVTTTIEYQ VKDMSLASLG RKRIEMAEKE MPGLMACRAK YGEEKPLNGV RITGSLHMTV ETAVLIETLK AIGGNIRWCS CNIFSTQDDA AAAIAAANTP VFAWKGETLV EYWECTWKAI RFGPYQGPQL IVDDGGDATL LIHRGFQAEK EPSILDEDGG VEELRIVNNL LKRILKEEPG FFSKIVPDIK GVSEETTTGV HRLYAMQKQG TLLFPAINVN DSVTKSKFDN IYGCRHSLLD GLNRATDVML AGKECVVCGF GDVGKGCAEA LKGQGARVIV TEIDPINALQ ACMAGYTVKT VEDCLATADI YVTATGNKDI ITLDHMKKMK DMAIICNIGH FDNEIDVLGL KTCEGVKEIN IKPQVDQFLF PDGHSIILLA QGRLVNLGCA TGHPAFVMSA SFTNQTLAQI SLWKDKYEIG VYTLPKVLDE EVARLHLEKL GAKLTKLTTS QADYIGVNAN GP
Validated NT Sequence
gtatgggccg ttagcattga caccaatgta gtcagcttga gatgttgtca acttggtcaa cttagcacca agcttctcca agtgaagtct ggcaacttcc tcgtccaaaa cctttggcaa agtgtagaca ccaatttcat acttgtcctt ccacaaggag atttgagcca atgtttggtt ggtgaatgaa gcagacatga cgaaagcagg gtgaccagta gcacaaccca aattgaccaa acgtccttga gccaacaaaa tgatggagtg tccatcaggg aacaagaatt gatcgacttg aggcttaatg ttaatttcct taacaccttc acaggtcttc aaaccgagga catcaatttc attgtcaaag tgaccaatgt tacaaatgat agccatatcc ttcatcttct tcatgtgatc gagagtaatg atatccttgt taccagtagc agtgacatag atatcagcag tagccaaaca atcttcaaca gtcttgacag tgtaaccagc catacaagct tggagagcgt taattgggtc aatttcagta acaataactc tggcaccttg tcccttcaaa gcttcagcgc atcctttacc gacatcaccg aaaccacaga caacacactc cttaccagcc aacatgacat cagtagctct gttcaaacca tccaacaagg agtgacggca tccgtaaatg ttgtcgaact tgctcttagt aacagagtcg ttcacgttaa tagcagggaa caacaaagta ccttgctttt gcatagcata caatctgtga acaccagtgg tggtctcttc ggagacaccc ttgatatctg gaacaatctt gctgaagaat ccaggctctt ccttcaaaat tctctttaac aagttattga caattctcaa ttcttcaaca ccaccatctt catccaaaat gcttggttcc ttttcagctt ggaatcctct gtgaatcaac aaggtagcat caccaccatc gtcgacaatc aattgtggtc cttgatatgg tccgaatcta atggccttcc aggtacattc ccaatattca accaaagtct ctcccttcca agcaaagact ggagtattag cagcagcaat ggcagcagcg gcatcgtctt gagtggagaa gatgttacaa ctgcaccaac ggatgttacc gccgatggcc ttgagagttt caataagaac agcagtttca acggtcatgt ggagacttcc ggtgattctg actccgttca atggcttctc ttcaccgtac ttggctctgc aagccatcaa accaggcatt tccttttcag ccatttcaat tctcttacgg cccaaagaag caaggctcat gtccttgact tggtattcaa tagtggtggt cactgtttgg ctcatatggt ggtggtggtg gtgagccat
Expressed Protein Sequence
MAHHHHHHMS QTVTTTIEYQ VKDMSLASLG RKRIEMAEKE MPGLMACRAK YGEEKPLNGV RITGSLHMTV ETAVLIETLK AIGGNIRWCS CNIFSTQDDA AAAIAAANTP VFAWKGETLV EYWECTWKAI RFGPYQGPQL IVDDGGDATL LIHRGFQAEK EPSILDEDGG VEELRIVNNL LKRILKEEPG FFSKIVPDIK GVSEETTTGV HRLYAMQKQG TLLFPAINVN DSVTKSKFDN IYGCRHSLLD GLNRATDVML AGKECVICGF GDVGKGCAEA LKGQGARVIV TEIDPINALQ ACMAGYTVKT VEDCLATADI YVTATGNKDI ITLDHMKKMK DMAIICNIGH FDNEIDVLGL KTCEGVKEIN IKPQVDQFLF PDGHSIILLA QGRLVNLGCA TGHPAFVMSA SFTNQTLAQI SLWKDKYEIG VYTLPKVLDE EVARLHLEKL GAKLTKLTTS QADYIGVNAN GPYKADHYRY
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAGCC AAACAGTGAC CACCACTATT GAATACCAAG TCAAGGACAT GAGCCTTGCT TCTTTGGGCC GTAAGAGAAT TGAAATGGCT GAAAAGGAAA TGCCTGGTTT GATGGCTTGC AGAGCCAAGT ACGGTGAAGA GAAGCCATTG AACGGAGTCA GAATCACCGG AAGTCTCCAC ATGACCGTTG AAACTGCTGT TCTTATTGAA ACTCTCAAGG CCATCGGCGG TAACATCCGT TGGTGCAGTT GTAACATCTT CTCCACTCAA GACGATGCCG CTGCTGCCAT TGCTGCTGCT AATACTCCAG TCTTTGCTTG GAAGGGAGAG ACTTTGGTTG AATATTGGGA ATGTACCTGG AAGGCCATTA GATTCGGACC ATATCAAGGA CCACAATTGA TTGTCGACGA TGGTGGTGAT GCTACCTTGT TGATTCACAG AGGATTCCAA GCTGAAAAGG AACCAAGCAT TTTGGATGAA GATGGTGGTG TTGAAGAATT GAGAATTGTC AATAACTTGT TAAAGAGAAT TTTGAAGGAA GAGCCTGGAT TCTTCAGCAA GATTGTTCCA GATATCAAGG GTGTCTCCGA AGAGACCACC ACTGGTGTTC ACAGATTGTA TGCTATGCAA AAGCAAGGTA CTTTGTTGTT CCCTGCTATT AACGTGAACG ACTCTGTTAC TAAGAGCAAG TTCGACAACA TTTACGGATG CCGTCACTCC TTGTTGGATG GTTTGAACAG AGCTACTGAT GTCATGTTGG CTGGTAAGGA GTGTGTTATC TGTGGTTTCG GTGATGTCGG TAAAGGATGC GCTGAAGCTT TGAAGGGACA AGGTGCCAGA GTTATTGTTA CTGAAATTGA CCCAATTAAC GCTCTCCAAG CTTGTATGGC TGGTTACACT GTCAAGACTG TTGAAGATTG TTTGGCTACT GCTGATATCT ATGTCACTGC TACTGGTAAC AAGGATATCA TTACTCTCGA TCACATGAAG AAGATGAAGG ATATGGCTAT CATTTGTAAC ATTGGTCACT TTGACAATGA AATTGATGTC CTCGGTTTGA AGACCTGTGA AGGTGTTAAG GAAATTAACA TTAAGCCTCA AGTCGATCAA TTCTTGTTCC CTGATGGACA CTCCATCATT TTGTTGGCTC AAGGACGTTT GGTCAATTTG GGTTGTGCTA CTGGTCACCC TGCTTTCGTC ATGTCTGCTT CATTCACCAA CCAAACATTG GCTCAAATCT CCTTGTGGAA GGACAAGTAT GAAATTGGTG TCTACACTTT GCCAAAGGTT TTGGACGAGG AAGTTGCCAG ACTTCACTTG GAGAAGCTTG GTGCTAAGTT GACCAAGTTG ACAACATCTC AAGCTGACTA CATTGGTGTC AATGCTAACG GCCCATACAA GGCAGATCAC TACAGATATT GAGTAAGATA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAATTCTT GAAGACGAAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTGTTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGCAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC TGATGCGGTA TTTTCTCCTT ACGCATCTGT GCGGTATTTC ACACCGCATA TATGGTGCAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG TATACACTCC GCTATCGCTA CGTGACTGGG TCATGGCTGC GCCCCGACAC CCGCCAACAC CCGCTGACGC GCCCTGACGG GCTTGTCTGC TCCCGGCATC CGCTTACAGA CAAGCTGTGA CCGTCTCCGG GAGCTGCATG TGTCAGAGGT TTTCACCGTC ATCACCGAAA CGCGCGAGGC AGCTGCGGTA AAGCTCATCA GCGTGGTCGT GAAGCGATTC ACAGATGTCT GCCTGTTCAT CCGCGTCCAG CTCGTTGAGT TTCTCCAGAA GCGTTAATGT CTGGCTTCTG ATAAAGCGGG CCATGTTAAG GGCGGTTTTT TCCTGTTTGG TCACTGATGC CTCCGTGTAA GGGGGATTTC TGTTCATGGG GGTAATGATA CCGATGAAAC GAGAGAGGAT GCTCACGATA CGGGTTACTG ATGATGAACA TGCCCGGTTA CTGGAACGTT GTGAGGGTAA ACAACTGGCG GTATGGATGC GGCGGGACCA GAGAAAAATC ACTCAGGGTC AATGCCAGCG CTTCGTTAAT ACAGATGTAG GTGTTCCACA GGGTAGCCAG CAGCATCCTG CGATGCAGAT CCGGAACATA ATGGTGCAGG GCGCTGACTT CCGCGTTTCC AGACTTTACG AAACACGGAA ACCGAAGACC ATTCATGTTG TTGCTCAGGT CGCAGACGTT TTGCAGCAGC AGTCGCTTCA CGTTCGCTCG CGTATCGGTG ATTCATTCTG CTAACCAGTA AGGCAACCCC GCCAGCCTAG CCGGGTCCTC AACGACAGGA GCACGATCAT GCGCACCCGT GGCCAGGACC CAACGCTGCC CGAGATGCGC CGCGTGCGGC TGCTGGAGAT GGCGGACGCG ATGGATATGT TCTGCCAAGG GTTGGTTTGC GCATTCACAG TTCTCCGCAA GAATTGATTG GCTCCAATTC TTGGAGTGGT GAATCCGTTA GCGAGGTGCC GCCGGCTTCC ATTCAGGTCG AGGTGGCCCG GCTCCATGCA CCGCGACGCA ACGCGGGGAG GCAGACAAGG TATAGGGCGG CGCCTACAAT CCATGCCAAC CCGTTCCATG TGCTCGCCGA GGCGGCATAA ATCGCCGTGA CGATCAGCGG TCCAGTGATC GAAGTTAGGC TGGTAAGAGC CGCGAGCGAT CCTTGAAGCT GTCCCTGATG GTCGTCATCT ACCTGCCTGG ACAGCATGGC CTGCAACGCG GGCATCCCGA TGCCGCCGGA AGCGAGAAGA ATCATAATGG GGAAGGCCAT CCAGCCTCGC GTCGCGAACG CCAGCAAGAC GTAGCCCAGC GCGTCGGCCG CCATGCCGGC GATAATGGCC TGCTTCTCGC CGAAACGTTT GGTGGCGGGA CCAGTGACGA AGGCTTGAGC GAGGGCGTGC AAGATTCCGA ATACCGCAAG CGACAGGCCG ATCATCGTCG CGCTCCAGCG AAAGCGGTCC TCGCCGAAAA TGACCCAGAG CGCTGCCGGC ACCTGTCCTA CGAGTTGCAT GATAAAGAAG ACAGTCATAA GTGCGGCGAC GATAGTCATG CCCCGCGCCC ACCGGAAGGA GCTGACTGGG TTGAAGGCTC TCAAGGGCAT CGGTCGACGC TCTCCCTTAT GCGACTCCTG CATTAGGAAG CAGCCCAGTA GTAGGTTGAG GCCGTTGAGC ACCGCCGCCG CAAGGAATGG TGCATGCAAG GAGATGGCGC CCAACAGTCC CCCGGCCACG GGGCCTGCCA CCATACCCAC GCCGAAACAA GCGCTCATGA GCCCGAAGTG GCGAGCCCGA TCTTCCCCAT CGGTGATGTC GGCGATATAG GCGCCAGCAA CCGCACCTGT GGCGCCGGTG ATGCCGGCCA CGATGCGTCC GGCGTAGAGG ATCGAGATCT CGATCCCGCG AAAT
Details for NafoA.00032.a.B1.PW38615
| PURIFICATION DATe: | 5/14/2019 |
| CONCENTRATION: | 45.8mg/ml |
| OBSERVED MW: | 53kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | 25 mM HEPES pH 7.0, 500 mM NaCl, 5% Glycerol , 2 mM DTT, 0.025% Azide |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 6 |
| VIAL VOLUME: | 110µl |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 98 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHMS QTVTTTIEYQ VKDMSLASLG RKRIEMAEKE MPGLMACRAK YGEEKPLNGV RITGSLHMTV ETAVLIETLK AIGGNIRWCS CNIFSTQDDA AAAIAAANTP VFAWKGETLV EYWECTWKAI RFGPYQGPQL IVDDGGDATL LIHRGFQAEK EPSILDEDGG VEELRIVNNL LKRILKEEPG FFSKIVPDIK GVSEETTTGV HRLYAMQKQG TLLFPAINVN DSVTKSKFDN IYGCRHSLLD GLNRATDVML AGKECVVCGF GDVGKGCAEA LKGQGARVIV TEIDPINALQ ACMAGYTVKT VEDCLATADI YVTATGNKDI ITLDHMKKMK DMAIICNIGH FDNEIDVLGL KTCEGVKEIN IKPQVDQFLF PDGHSIILLA QGRLVNLGCA TGHPAFVMSA SFTNQTLAQI SLWKDKYEIG VYTLPKVLDE EVARLHLEKL GAKLTKLTTS QADYIGVNAN GP
Validated NT Sequence
gtatgggccg ttagcattga caccaatgta gtcagcttga gatgttgtca acttggtcaa cttagcacca agcttctcca agtgaagtct ggcaacttcc tcgtccaaaa cctttggcaa agtgtagaca ccaatttcat acttgtcctt ccacaaggag atttgagcca atgtttggtt ggtgaatgaa gcagacatga cgaaagcagg gtgaccagta gcacaaccca aattgaccaa acgtccttga gccaacaaaa tgatggagtg tccatcaggg aacaagaatt gatcgacttg aggcttaatg ttaatttcct taacaccttc acaggtcttc aaaccgagga catcaatttc attgtcaaag tgaccaatgt tacaaatgat agccatatcc ttcatcttct tcatgtgatc gagagtaatg atatccttgt taccagtagc agtgacatag atatcagcag tagccaaaca atcttcaaca gtcttgacag tgtaaccagc catacaagct tggagagcgt taattgggtc aatttcagta acaataactc tggcaccttg tcccttcaaa gcttcagcgc atcctttacc gacatcaccg aaaccacaga caacacactc cttaccagcc aacatgacat cagtagctct gttcaaacca tccaacaagg agtgacggca tccgtaaatg ttgtcgaact tgctcttagt aacagagtcg ttcacgttaa tagcagggaa caacaaagta ccttgctttt gcatagcata caatctgtga acaccagtgg tggtctcttc ggagacaccc ttgatatctg gaacaatctt gctgaagaat ccaggctctt ccttcaaaat tctctttaac aagttattga caattctcaa ttcttcaaca ccaccatctt catccaaaat gcttggttcc ttttcagctt ggaatcctct gtgaatcaac aaggtagcat caccaccatc gtcgacaatc aattgtggtc cttgatatgg tccgaatcta atggccttcc aggtacattc ccaatattca accaaagtct ctcccttcca agcaaagact ggagtattag cagcagcaat ggcagcagcg gcatcgtctt gagtggagaa gatgttacaa ctgcaccaac ggatgttacc gccgatggcc ttgagagttt caataagaac agcagtttca acggtcatgt ggagacttcc ggtgattctg actccgttca atggcttctc ttcaccgtac ttggctctgc aagccatcaa accaggcatt tccttttcag ccatttcaat tctcttacgg cccaaagaag caaggctcat gtccttgact tggtattcaa tagtggtggt cactgtttgg ctcatatggt ggtggtggtg gtgagccat
Expressed Protein Sequence
MAHHHHHHMS QTVTTTIEYQ VKDMSLASLG RKRIEMAEKE MPGLMACRAK YGEEKPLNGV RITGSLHMTV ETAVLIETLK AIGGNIRWCS CNIFSTQDDA AAAIAAANTP VFAWKGETLV EYWECTWKAI RFGPYQGPQL IVDDGGDATL LIHRGFQAEK EPSILDEDGG VEELRIVNNL LKRILKEEPG FFSKIVPDIK GVSEETTTGV HRLYAMQKQG TLLFPAINVN DSVTKSKFDN IYGCRHSLLD GLNRATDVML AGKECVICGF GDVGKGCAEA LKGQGARVIV TEIDPINALQ ACMAGYTVKT VEDCLATADI YVTATGNKDI ITLDHMKKMK DMAIICNIGH FDNEIDVLGL KTCEGVKEIN IKPQVDQFLF PDGHSIILLA QGRLVNLGCA TGHPAFVMSA SFTNQTLAQI SLWKDKYEIG VYTLPKVLDE EVARLHLEKL GAKLTKLTTS QADYIGVNAN GPYKADHYRY
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAGCC AAACAGTGAC CACCACTATT GAATACCAAG TCAAGGACAT GAGCCTTGCT TCTTTGGGCC GTAAGAGAAT TGAAATGGCT GAAAAGGAAA TGCCTGGTTT GATGGCTTGC AGAGCCAAGT ACGGTGAAGA GAAGCCATTG AACGGAGTCA GAATCACCGG AAGTCTCCAC ATGACCGTTG AAACTGCTGT TCTTATTGAA ACTCTCAAGG CCATCGGCGG TAACATCCGT TGGTGCAGTT GTAACATCTT CTCCACTCAA GACGATGCCG CTGCTGCCAT TGCTGCTGCT AATACTCCAG TCTTTGCTTG GAAGGGAGAG ACTTTGGTTG AATATTGGGA ATGTACCTGG AAGGCCATTA GATTCGGACC ATATCAAGGA CCACAATTGA TTGTCGACGA TGGTGGTGAT GCTACCTTGT TGATTCACAG AGGATTCCAA GCTGAAAAGG AACCAAGCAT TTTGGATGAA GATGGTGGTG TTGAAGAATT GAGAATTGTC AATAACTTGT TAAAGAGAAT TTTGAAGGAA GAGCCTGGAT TCTTCAGCAA GATTGTTCCA GATATCAAGG GTGTCTCCGA AGAGACCACC ACTGGTGTTC ACAGATTGTA TGCTATGCAA AAGCAAGGTA CTTTGTTGTT CCCTGCTATT AACGTGAACG ACTCTGTTAC TAAGAGCAAG TTCGACAACA TTTACGGATG CCGTCACTCC TTGTTGGATG GTTTGAACAG AGCTACTGAT GTCATGTTGG CTGGTAAGGA GTGTGTTATC TGTGGTTTCG GTGATGTCGG TAAAGGATGC GCTGAAGCTT TGAAGGGACA AGGTGCCAGA GTTATTGTTA CTGAAATTGA CCCAATTAAC GCTCTCCAAG CTTGTATGGC TGGTTACACT GTCAAGACTG TTGAAGATTG TTTGGCTACT GCTGATATCT ATGTCACTGC TACTGGTAAC AAGGATATCA TTACTCTCGA TCACATGAAG AAGATGAAGG ATATGGCTAT CATTTGTAAC ATTGGTCACT TTGACAATGA AATTGATGTC CTCGGTTTGA AGACCTGTGA AGGTGTTAAG GAAATTAACA TTAAGCCTCA AGTCGATCAA TTCTTGTTCC CTGATGGACA CTCCATCATT TTGTTGGCTC AAGGACGTTT GGTCAATTTG GGTTGTGCTA CTGGTCACCC TGCTTTCGTC ATGTCTGCTT CATTCACCAA CCAAACATTG GCTCAAATCT CCTTGTGGAA GGACAAGTAT GAAATTGGTG TCTACACTTT GCCAAAGGTT TTGGACGAGG AAGTTGCCAG ACTTCACTTG GAGAAGCTTG GTGCTAAGTT GACCAAGTTG ACAACATCTC AAGCTGACTA CATTGGTGTC AATGCTAACG GCCCATACAA GGCAGATCAC TACAGATATT GAGTAAGATA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAATTCTT GAAGACGAAA GGGCCTCGTG ATACGCCTAT TTTTATAGGT TAATGTCATG ATAATAATGG TTTCTTAGAC GTCAGGTGGC ACTTTTCGGG GAAATGTGCG CGGAACCCCT ATTTGTTTAT TTTTCTAAAT ACATTCAAAT ATGTATCCGC TCATGAGACA ATAACCCTGA TAAATGCTTC AATAATATTG AAAAAGGAAG AGTATGAGTA TTCAACATTT CCGTGTCGCC CTTATTCCCT TTTTTGCGGC ATTTTGCCTT CCTGTTTTTG CTCACCCAGA AACGCTGGTG AAAGTAAAAG ATGCTGAAGA TCAGTTGGGT GCACGAGTGG GTTACATCGA ACTGGATCTC AACAGCGGTA AGATCCTTGA GAGTTTTCGC CCCGAAGAAC GTTTTCCAAT GATGAGCACT TTTAAAGTTC TGCTATGTGG CGCGGTATTA TCCCGTGTTG ACGCCGGGCA AGAGCAACTC GGTCGCCGCA TACACTATTC TCAGAATGAC TTGGTTGAGT ACTCACCAGT CACAGAAAAG CATCTTACGG ATGGCATGAC AGTAAGAGAA TTATGCAGTG CTGCCATAAC CATGAGTGAT AACACTGCGG CCAACTTACT TCTGACAACG ATCGGAGGAC CGAAGGAGCT AACCGCTTTT TTGCACAACA TGGGGGATCA TGTAACTCGC CTTGATCGTT GGGAACCGGA GCTGAATGAA GCCATACCAA ACGACGAGCG TGACACCACG ATGCCTGCAG CAATGGCAAC AACGTTGCGC AAACTATTAA CTGGCGAACT ACTTACTCTA GCTTCCCGGC AACAATTAAT AGACTGGATG GAGGCGGATA AAGTTGCAGG ACCACTTCTG CGCTCGGCCC TTCCGGCTGG CTGGTTTATT GCTGATAAAT CTGGAGCCGG TGAGCGTGGG TCTCGCGGTA TCATTGCAGC ACTGGGGCCA GATGGTAAGC CCTCCCGTAT CGTAGTTATC TACACGACGG GGAGTCAGGC AACTATGGAT GAACGAAATA GACAGATCGC TGAGATAGGT GCCTCACTGA TTAAGCATTG GTAACTGTCA GACCAAGTTT ACTCATATAT ACTTTAGATT GATTTAAAAC TTCATTTTTA ATTTAAAAGG ATCTAGGTGA AGATCCTTTT TGATAATCTC ATGACCAAAA TCCCTTAACG TGAGTTTTCG TTCCACTGAG CGTCAGACCC CGTAGAAAAG ATCAAAGGAT CTTCTTGAGA TCCTTTTTTT CTGCGCGTAA TCTGCTGCTT GCAAACAAAA AAACCACCGC TACCAGCGGT GGTTTGTTTG CCGGATCAAG AGCTACCAAC TCTTTTTCCG AAGGTAACTG GCTTCAGCAG AGCGCAGATA CCAAATACTG TCCTTCTAGT GTAGCCGTAG TTAGGCCACC ACTTCAAGAA CTCTGTAGCA CCGCCTACAT ACCTCGCTCT GCTAATCCTG TTACCAGTGG CTGCTGCCAG TGGCGATAAG TCGTGTCTTA CCGGGTTGGA CTCAAGACGA TAGTTACCGG ATAAGGCGCA GCGGTCGGGC TGAACGGGGG GTTCGTGCAC ACAGCCCAGC TTGGAGCGAA CGACCTACAC CGAACTGAGA TACCTACAGC GTGAGCTATG AGAAAGCGCC ACGCTTCCCG AAGGGAGAAA GGCGGACAGG TATCCGGTAA GCGGCAGGGT CGGAACAGGA GAGCGCACGA GGGAGCTTCC AGGGGGAAAC GCCTGGTATC TTTATAGTCC TGTCGGGTTT CGCCACCTCT GACTTGAGCG TCGATTTTTG TGATGCTCGT CAGGGGGGCG GAGCCTATGG AAAAACGCCA GCAACGCGGC CTTTTTACGG TTCCTGGCCT TTTGCTGGCC TTTTGCTCAC ATGTTCTTTC CTGCGTTATC CCCTGATTCT GTGGATAACC GTATTACCGC CTTTGAGTGA GCTGATACCG CTCGCCGCAG CCGAACGACC GAGCGCAGCG AGTCAGTGAG CGAGGAAGCG GAAGAGCGCC TGATGCGGTA TTTTCTCCTT ACGCATCTGT GCGGTATTTC ACACCGCATA TATGGTGCAC TCTCAGTACA ATCTGCTCTG ATGCCGCATA GTTAAGCCAG TATACACTCC GCTATCGCTA CGTGACTGGG TCATGGCTGC GCCCCGACAC CCGCCAACAC CCGCTGACGC GCCCTGACGG GCTTGTCTGC TCCCGGCATC CGCTTACAGA CAAGCTGTGA CCGTCTCCGG GAGCTGCATG TGTCAGAGGT TTTCACCGTC ATCACCGAAA CGCGCGAGGC AGCTGCGGTA AAGCTCATCA GCGTGGTCGT GAAGCGATTC ACAGATGTCT GCCTGTTCAT CCGCGTCCAG CTCGTTGAGT TTCTCCAGAA GCGTTAATGT CTGGCTTCTG ATAAAGCGGG CCATGTTAAG GGCGGTTTTT TCCTGTTTGG TCACTGATGC CTCCGTGTAA GGGGGATTTC TGTTCATGGG GGTAATGATA CCGATGAAAC GAGAGAGGAT GCTCACGATA CGGGTTACTG ATGATGAACA TGCCCGGTTA CTGGAACGTT GTGAGGGTAA ACAACTGGCG GTATGGATGC GGCGGGACCA GAGAAAAATC ACTCAGGGTC AATGCCAGCG CTTCGTTAAT ACAGATGTAG GTGTTCCACA GGGTAGCCAG CAGCATCCTG CGATGCAGAT CCGGAACATA ATGGTGCAGG GCGCTGACTT CCGCGTTTCC AGACTTTACG AAACACGGAA ACCGAAGACC ATTCATGTTG TTGCTCAGGT CGCAGACGTT TTGCAGCAGC AGTCGCTTCA CGTTCGCTCG CGTATCGGTG ATTCATTCTG CTAACCAGTA AGGCAACCCC GCCAGCCTAG CCGGGTCCTC AACGACAGGA GCACGATCAT GCGCACCCGT GGCCAGGACC CAACGCTGCC CGAGATGCGC CGCGTGCGGC TGCTGGAGAT GGCGGACGCG ATGGATATGT TCTGCCAAGG GTTGGTTTGC GCATTCACAG TTCTCCGCAA GAATTGATTG GCTCCAATTC TTGGAGTGGT GAATCCGTTA GCGAGGTGCC GCCGGCTTCC ATTCAGGTCG AGGTGGCCCG GCTCCATGCA CCGCGACGCA ACGCGGGGAG GCAGACAAGG TATAGGGCGG CGCCTACAAT CCATGCCAAC CCGTTCCATG TGCTCGCCGA GGCGGCATAA ATCGCCGTGA CGATCAGCGG TCCAGTGATC GAAGTTAGGC TGGTAAGAGC CGCGAGCGAT CCTTGAAGCT GTCCCTGATG GTCGTCATCT ACCTGCCTGG ACAGCATGGC CTGCAACGCG GGCATCCCGA TGCCGCCGGA AGCGAGAAGA ATCATAATGG GGAAGGCCAT CCAGCCTCGC GTCGCGAACG CCAGCAAGAC GTAGCCCAGC GCGTCGGCCG CCATGCCGGC GATAATGGCC TGCTTCTCGC CGAAACGTTT GGTGGCGGGA CCAGTGACGA AGGCTTGAGC GAGGGCGTGC AAGATTCCGA ATACCGCAAG CGACAGGCCG ATCATCGTCG CGCTCCAGCG AAAGCGGTCC TCGCCGAAAA TGACCCAGAG CGCTGCCGGC ACCTGTCCTA CGAGTTGCAT GATAAAGAAG ACAGTCATAA GTGCGGCGAC GATAGTCATG CCCCGCGCCC ACCGGAAGGA GCTGACTGGG TTGAAGGCTC TCAAGGGCAT CGGTCGACGC TCTCCCTTAT GCGACTCCTG CATTAGGAAG CAGCCCAGTA GTAGGTTGAG GCCGTTGAGC ACCGCCGCCG CAAGGAATGG TGCATGCAAG GAGATGGCGC CCAACAGTCC CCCGGCCACG GGGCCTGCCA CCATACCCAC GCCGAAACAA GCGCTCATGA GCCCGAAGTG GCGAGCCCGA TCTTCCCCAT CGGTGATGTC GGCGATATAG GCGCCAGCAA CCGCACCTGT GGCGCCGGTG ATGCCGGCCA CGATGCGTCC GGCGTAGAGG ATCGAGATCT CGATCCCGCG AAAT