NafoA.00008.a

serine mitochondrial-like

CENTER ID: NafoA.00008.a
ORGANISM: Naegleria fowleri ATCC 30863
ASSOCIATED DISEASE:
CURRENT STATUS: diffraction
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
NafoA.00008.a.B1.GE40137 Full length(NafoA.00008.a) 1 505
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
NafoA.00008.a.B1.PS38181 Full length(NafoA.00008.a) 1 505
External Resources
RESOURCE REFERENCE ID
EuPathDB: AmoebaDB:NF0102130
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MLHRPSALRA TCNSIINHTN TARRNFSSSS LVSAPTSGTV NTEKWAQQLN ASLKDTDPEM FDIIEHEKNR QYKGLQLIPS ENFTSKAVLD ALGSVMQNKY SEGYPGKRYY GGNEFIDMSE SLCQKRALEA FNLDPNEWGV NVQSLSGSPA NFYVYTALLQ PHDRILSLDL PHGGHLSHGY QTPTKKISAV SIYFETMGYR LNEETGLIDY AKMEELADYY RPKLVVAGAS AYSRLIDYAF FRKVCDKHNA YLVSDMAHIS GLVAAGVIPS PFEHSHVVTT TTHKSLRGPR GAMIFYRKSI EGLEEKINSA VFPGHQGGPH NHTISALAVA LKQAITPEYK EYQQQVLKNC KVLTEEMKKL GYKIVSDGTD NHLLLVDLRN KGVDGARVEA VLERSSIAVN KNTVPGDKSA MVPGGIRMGS PALTTRGFLE KDFAQVAQFV HEGVQIAIKT KSELDQKGLK KVKDFKDYLD QNPDSVGITA LKSKVETFSK SFPTIGFDKD SMKYK
NT Sequence
ATGTTGCATC GTCCATCTGC GTTGCGTGCC ACCTGCAATT CCATCATCAA CCACACCAAT ACTGCCCGTA GAAATTTCTC TTCATCAAGC CTGGTTTCAG CTCCAACATC GGGAACAGTG AATACCGAGA AATGGGCACA ACAATTAAAT GCATCCTTGA AGGATACTGA TCCTGAAATG TTTGATATTA TTGAACACGA GAAGAATAGA CAGTATAAGG GATTACAATT GATTCCATCA GAGAACTTTA CTTCAAAAGC TGTATTGGAT GCATTGGGAA GTGTCATGCA AAACAAGTAC AGCGAGGGAT ATCCAGGAAA GCGTTATTAT GGTGGCAATG AGTTTATTGA CATGTCTGAA AGTTTGTGCC AAAAGAGAGC TCTTGAAGCT TTTAATTTGG ATCCAAATGA ATGGGGAGTC AATGTTCAAA GTTTGAGCGG TTCACCAGCC AACTTTTATG TTTACACTGC ATTGCTTCAA CCACACGATA GAATTCTTTC CTTGGATTTA CCACACGGAG GACACCTTTC TCACGGTTAT CAAACTCCAA CCAAGAAGAT CTCTGCAGTC AGTATTTACT TTGAAACCAT GGGCTATCGA TTGAATGAAG AAACAGGTTT AATTGACTAT GCCAAGATGG AAGAATTGGC TGATTACTAC AGACCAAAAT TAGTTGTTGC TGGAGCAAGT GCTTATTCTC GTTTGATTGA TTATGCTTTC TTTAGAAAAG TCTGTGATAA GCACAATGCC TATCTCGTTT CTGACATGGC TCACATTAGT GGTCTTGTTG CAGCAGGTGT AATTCCATCT CCATTTGAGC ATTCTCATGT GGTGACTACC ACCACTCACA AGAGTTTGAG AGGTCCAAGA GGAGCCATGA TTTTCTACAG AAAGAGTATT GAAGGTCTCG AAGAAAAGAT CAACTCTGCT GTCTTTCCAG GTCATCAAGG TGGTCCTCAC AATCACACCA TCTCTGCATT GGCTGTTGCC CTTAAACAAG CAATCACACC AGAATATAAG GAATATCAAC AACAAGTATT GAAGAATTGC AAAGTCTTGA CTGAAGAAAT GAAGAAACTC GGTTACAAGA TTGTGAGCGA TGGAACTGAT AACCATTTGC TTCTCGTCGA CTTGAGAAAT AAGGGAGTCG ATGGAGCTCG TGTTGAAGCT GTTCTCGAAC GTTCTAGCAT TGCTGTCAAT AAGAACACTG TTCCAGGTGA TAAATCTGCC ATGGTTCCAG GAGGTATTCG TATGGGATCT CCTGCTCTCA CCACTCGAGG ATTTTTGGAG AAGGATTTTG CACAAGTAGC TCAGTTTGTG CATGAAGGTG TTCAAATTGC TATCAAGACC AAGAGTGAAT TGGATCAAAA AGGTTTGAAG AAAGTGAAGG ACTTTAAGGA TTATTTGGAT CAAAATCCAG ATTCTGTTGG AATTACTGCA TTGAAGAGTA AGGTTGAGAC CTTTAGCAAA TCATTCCCAA CCATTGGTTT TGATAAGGAT TCAATGAAAT ACAAG
Details for NafoA.00008.a.B1.GE40137
HARVESTED ON: 4/6/2016
SEQUENCED ON: 4/13/2016
EXPECTED MW: 57kDa
OBSERVED MW: 57kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHML HRPSALRATC NSIINHTNTA RRNFSSSSLV SAPTSGTVNT EKWAQQLNAS LKDTDPEMFD IIEHEKNRQY KGLQLIPSEN FTSKAVLDAL GSVMQNKYSE GYPGKRYYGG NEFIDMSESL CQKRALEAFN LDPNEWGVNV QSLSGSPANF YVYTALLQPH DRILSLDLPH GGHLSHGYQT PTKKISAVSI YFETMGYRLN EETGLIDYAK MEELADYYRP KLVVAGASAY SRLIDYAFFR KVCDKHNAYL VSDMAHISGL VAAGVIPSPF EHSHVVTTTT HKSLRGPRGA MIFYRKSIEG LEEKINSAVF PGHQGGPHNH TISALAVALK QAITPEYKEY QQQVLKNCKV LTEEMKKLGY KIVSDGTDNH LLLVDLRNKG VDGARVEAVL ERSSIAVNKN TVPGDKSAMV PGGIRMGSPA LTTRGFLEKD FAQVAQFVHE GVQIAIKTKS ELDQKGLKKV KDFKDYLDQN PDSVGITALK SKVETFSKSF PTIG
Validated NT Sequence
aaaccaatgg ttgggaatga tttgctaaag gtctcaacct tactcttcaa tgcagtaatt ccaacagaat ctggattttg atccaaataa tccttaaagt ccttcacttt cttcaaacct ttttgatcca attcactctt ggtcttgata gcaatttgaa caccttcatg cacaaactga gctacttgtg caaaatcctt ctccaaaaat cctcgagtgg tgagagcagg agatcccata cgaatacctc ctggaaccat ggcagattta tcacctggaa cagtgttctt attgacagca atgctagaac gttcgagaac agcttcaaca cgagctccat cgactccctt atttctcaag tcgacgagaa gcaaatggtt atcagttcca tcgctcacaa tcttgtaacc gagtttcttc atttcttcag tcaagacttt gcaattcttc aatacttgtt gttgatattc cttatattct ggtgtgattg cttgtttaag ggcaacagcc aatgcagaga tggtgtgatt gtgaggacca ccttgatgac ctggaaagac agcagagttg atcttttctt cgagaccttc aatactcttt ctgtagaaaa tcatggctcc tcttggacct ctcaaactct tgtgagtggt ggtagtcacc acatgagaat gctcaaatgg agatggaatt acacctgctg caacaagacc actaatgtga gccatgtcag aaacgagata ggcattgtgc ttatcacaga cttttctaaa gaaagcataa tcaatcaaac gagaataagc acttgctcca gcaacaacta attttggtct gtagtaatca gccaattctt ccatcttggc atagtcaatt aaacctgttt cttcattcaa tcgatagccc atggtttcaa agtaaatact gactgcagag atcttcttgg ttggagtttg ataaccgtga gaaaggtgtc ctccgtgtgg taaatccaag gaaagaattc tatcgtgtgg ttgaagcaat gcagtgtaaa cataaaagtt ggctggtgaa ccgctcaaac tttgaacatt gactccccat tcatttggat ccaaattaaa agcttcaaga gctctctttt ggcacaaact ttcagacatg tcaataaact cattgccacc ataataacgc tttcctggat atccctcgct gtacttgttt tgcatgacac ttcccaatgc atccaataca gcttttgaag taaagttctc tgatggaatc aattgtaatc ccttatactg tctattcttc tcgtgttcaa taatatcaaa catttcagga tcagtatcct tcaaggatgc atttaattgt tgtgcccatt tctcggtatt cactgttccc gatgttggag ctgaaaccag gcttgatgaa gagaaatttc tacgggcagt attggtgtgg ttgatgatgg aattgcaggt ggcacgcaac gcagatggac gatgcaacat atggtggtgg tggtggtgag ccat
Expected Protein Sequence
MAHHHHHHML HRPSALRATC NSIINHTNTA RRNFSSSSLV SAPTSGTVNT EKWAQQLNAS LKDTDPEMFD IIEHEKNRQY KGLQLIPSEN FTSKAVLDAL GSVMQNKYSE GYPGKRYYGG NEFIDMSESL CQKRALEAFN LDPNEWGVNV QSLSGSPANF YVYTALLQPH DRILSLDLPH GGHLSHGYQT PTKKISAVSI YFETMGYRLN EETGLIDYAK MEELADYYRP KLVVAGASAY SRLIDYAFFR KVCDKHNAYL VSDMAHISGL VAAGVIPSPF EHSHVVTTTT HKSLRGPRGA MIFYRKSIEG LEEKINSAVF PGHQGGPHNH TISALAVALK QAITPEYKEY QQQVLKNCKV LTEEMKKLGY KIVSDGTDNH LLLVDLRNKG VDGARVEAVL ERSSIAVNKN TVPGDKSAMV PGGIRMGSPA LTTRGFLEKD FAQVAQFVHE GVQIAIKTKS ELDQKGLKKV KDFKDYLDQN PDSVGITALK SKVETFSKSF PTIGFDKDSM KYK
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgttgc atcgtccatc tgcgttgcgt gccacctgca attccatcat caaccacacc aatactgccc gtagaaattt ctcttcatca agcctggttt cagctccaac atcgggaaca gtgaataccg agaaatgggc acaacaatta aatgcatcct tgaaggatac tgatcctgaa atgtttgata ttattgaaca cgagaagaat agacagtata agggattaca attgattcca tcagagaact ttacttcaaa agctgtattg gatgcattgg gaagtgtcat gcaaaacaag tacagcgagg gatatccagg aaagcgttat tatggtggca atgagtttat tgacatgtct gaaagtttgt gccaaaagag agctcttgaa gcttttaatt tggatccaaa tgaatgggga gtcaatgttc aaagtttgag cggttcacca gccaactttt atgtttacac tgcattgctt caaccacacg atagaattct ttccttggat ttaccacacg gaggacacct ttctcacggt tatcaaactc caaccaagaa gatctctgca gtcagtattt actttgaaac catgggctat cgattgaatg aagaaacagg tttaattgac tatgccaaga tggaagaatt ggctgattac tacagaccaa aattagttgt tgctggagca agtgcttatt ctcgtttgat tgattatgct ttctttagaa aagtctgtga taagcacaat gcctatctcg tttctgacat ggctcacatt agtggtcttg ttgcagcagg tgtaattcca tctccatttg agcattctca tgtggtgact accaccactc acaagagttt gagaggtcca agaggagcca tgattttcta cagaaagagt attgaaggtc tcgaagaaaa gatcaactct gctgtctttc caggtcatca aggtggtcct cacaatcaca ccatctctgc attggctgtt gcccttaaac aagcaatcac accagaatat aaggaatatc aacaacaagt attgaagaat tgcaaagtct tgactgaaga aatgaagaaa ctcggttaca agattgtgag cgatggaact gataaccatt tgcttctcgt cgacttgaga aataagggag tcgatggagc tcgtgttgaa gctgttctcg aacgttctag cattgctgtc aataagaaca ctgttccagg tgataaatct gccatggttc caggaggtat tcgtatggga tctcctgctc tcaccactcg aggatttttg gagaaggatt ttgcacaagt agctcagttt gtgcatgaag gtgttcaaat tgctatcaag accaagagtg aattggatca aaaaggtttg aagaaagtga aggactttaa ggattatttg gatcaaaatc cagattctgt tggaattact gcattgaaga gtaaggttga gacctttagc aaatcattcc caaccattgg ttttgataag gattcaatga aatacaagtg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat
Details for NafoA.00008.a.B1.PS38181
PURIFICATION DATe: 1/19/2017
CONCENTRATION: 32.61mg/ml
OBSERVED MW: 48kDa
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: SEC
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 7
VIAL VOLUME: data unavailable
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHHML HRPSALRATC NSIINHTNTA RRNFSSSSLV SAPTSGTVNT EKWAQQLNAS LKDTDPEMFD IIEHEKNRQY KGLQLIPSEN FTSKAVLDAL GSVMQNKYSE GYPGKRYYGG NEFIDMSESL CQKRALEAFN LDPNEWGVNV QSLSGSPANF YVYTALLQPH DRILSLDLPH GGHLSHGYQT PTKKISAVSI YFETMGYRLN EETGLIDYAK MEELADYYRP KLVVAGASAY SRLIDYAFFR KVCDKHNAYL VSDMAHISGL VAAGVIPSPF EHSHVVTTTT HKSLRGPRGA MIFYRKSIEG LEEKINSAVF PGHQGGPHNH TISALAVALK QAITPEYKEY QQQVLKNCKV LTEEMKKLGY KIVSDGTDNH LLLVDLRNKG VDGARVEAVL ERSSIAVNKN TVPGDKSAMV PGGIRMGSPA LTTRGFLEKD FAQVAQFVHE GVQIAIKTKS ELDQKGLKKV KDFKDYLDQN PDSVGITALK SKVETFSKSF PTIG
Validated NT Sequence
aaaccaatgg ttgggaatga tttgctaaag gtctcaacct tactcttcaa tgcagtaatt ccaacagaat ctggattttg atccaaataa tccttaaagt ccttcacttt cttcaaacct ttttgatcca attcactctt ggtcttgata gcaatttgaa caccttcatg cacaaactga gctacttgtg caaaatcctt ctccaaaaat cctcgagtgg tgagagcagg agatcccata cgaatacctc ctggaaccat ggcagattta tcacctggaa cagtgttctt attgacagca atgctagaac gttcgagaac agcttcaaca cgagctccat cgactccctt atttctcaag tcgacgagaa gcaaatggtt atcagttcca tcgctcacaa tcttgtaacc gagtttcttc atttcttcag tcaagacttt gcaattcttc aatacttgtt gttgatattc cttatattct ggtgtgattg cttgtttaag ggcaacagcc aatgcagaga tggtgtgatt gtgaggacca ccttgatgac ctggaaagac agcagagttg atcttttctt cgagaccttc aatactcttt ctgtagaaaa tcatggctcc tcttggacct ctcaaactct tgtgagtggt ggtagtcacc acatgagaat gctcaaatgg agatggaatt acacctgctg caacaagacc actaatgtga gccatgtcag aaacgagata ggcattgtgc ttatcacaga cttttctaaa gaaagcataa tcaatcaaac gagaataagc acttgctcca gcaacaacta attttggtct gtagtaatca gccaattctt ccatcttggc atagtcaatt aaacctgttt cttcattcaa tcgatagccc atggtttcaa agtaaatact gactgcagag atcttcttgg ttggagtttg ataaccgtga gaaaggtgtc ctccgtgtgg taaatccaag gaaagaattc tatcgtgtgg ttgaagcaat gcagtgtaaa cataaaagtt ggctggtgaa ccgctcaaac tttgaacatt gactccccat tcatttggat ccaaattaaa agcttcaaga gctctctttt ggcacaaact ttcagacatg tcaataaact cattgccacc ataataacgc tttcctggat atccctcgct gtacttgttt tgcatgacac ttcccaatgc atccaataca gcttttgaag taaagttctc tgatggaatc aattgtaatc ccttatactg tctattcttc tcgtgttcaa taatatcaaa catttcagga tcagtatcct tcaaggatgc atttaattgt tgtgcccatt tctcggtatt cactgttccc gatgttggag ctgaaaccag gcttgatgaa gagaaatttc tacgggcagt attggtgtgg ttgatgatgg aattgcaggt ggcacgcaac gcagatggac gatgcaacat atggtggtgg tggtggtgag ccat
Expressed Protein Sequence
MAHHHHHHML HRPSALRATC NSIINHTNTA RRNFSSSSLV SAPTSGTVNT EKWAQQLNAS LKDTDPEMFD IIEHEKNRQY KGLQLIPSEN FTSKAVLDAL GSVMQNKYSE GYPGKRYYGG NEFIDMSESL CQKRALEAFN LDPNEWGVNV QSLSGSPANF YVYTALLQPH DRILSLDLPH GGHLSHGYQT PTKKISAVSI YFETMGYRLN EETGLIDYAK MEELADYYRP KLVVAGASAY SRLIDYAFFR KVCDKHNAYL VSDMAHISGL VAAGVIPSPF EHSHVVTTTT HKSLRGPRGA MIFYRKSIEG LEEKINSAVF PGHQGGPHNH TISALAVALK QAITPEYKEY QQQVLKNCKV LTEEMKKLGY KIVSDGTDNH LLLVDLRNKG VDGARVEAVL ERSSIAVNKN TVPGDKSAMV PGGIRMGSPA LTTRGFLEKD FAQVAQFVHE GVQIAIKTKS ELDQKGLKKV KDFKDYLDQN PDSVGITALK SKVETFSKSF PTIGFDKDSM KYK
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGTTGC ATCGTCCATC TGCGTTGCGT GCCACCTGCA ATTCCATCAT CAACCACACC AATACTGCCC GTAGAAATTT CTCTTCATCA AGCCTGGTTT CAGCTCCAAC ATCGGGAACA GTGAATACCG AGAAATGGGC ACAACAATTA AATGCATCCT TGAAGGATAC TGATCCTGAA ATGTTTGATA TTATTGAACA CGAGAAGAAT AGACAGTATA AGGGATTACA ATTGATTCCA TCAGAGAACT TTACTTCAAA AGCTGTATTG GATGCATTGG GAAGTGTCAT GCAAAACAAG TACAGCGAGG GATATCCAGG AAAGCGTTAT TATGGTGGCA ATGAGTTTAT TGACATGTCT GAAAGTTTGT GCCAAAAGAG AGCTCTTGAA GCTTTTAATT TGGATCCAAA TGAATGGGGA GTCAATGTTC AAAGTTTGAG CGGTTCACCA GCCAACTTTT ATGTTTACAC TGCATTGCTT CAACCACACG ATAGAATTCT TTCCTTGGAT TTACCACACG GAGGACACCT TTCTCACGGT TATCAAACTC CAACCAAGAA GATCTCTGCA GTCAGTATTT ACTTTGAAAC CATGGGCTAT CGATTGAATG AAGAAACAGG TTTAATTGAC TATGCCAAGA TGGAAGAATT GGCTGATTAC TACAGACCAA AATTAGTTGT TGCTGGAGCA AGTGCTTATT CTCGTTTGAT TGATTATGCT TTCTTTAGAA AAGTCTGTGA TAAGCACAAT GCCTATCTCG TTTCTGACAT GGCTCACATT AGTGGTCTTG TTGCAGCAGG TGTAATTCCA TCTCCATTTG AGCATTCTCA TGTGGTGACT ACCACCACTC ACAAGAGTTT GAGAGGTCCA AGAGGAGCCA TGATTTTCTA CAGAAAGAGT ATTGAAGGTC TCGAAGAAAA GATCAACTCT GCTGTCTTTC CAGGTCATCA AGGTGGTCCT CACAATCACA CCATCTCTGC ATTGGCTGTT GCCCTTAAAC AAGCAATCAC ACCAGAATAT AAGGAATATC AACAACAAGT ATTGAAGAAT TGCAAAGTCT TGACTGAAGA AATGAAGAAA CTCGGTTACA AGATTGTGAG CGATGGAACT GATAACCATT TGCTTCTCGT CGACTTGAGA AATAAGGGAG TCGATGGAGC TCGTGTTGAA GCTGTTCTCG AACGTTCTAG CATTGCTGTC AATAAGAACA CTGTTCCAGG TGATAAATCT GCCATGGTTC CAGGAGGTAT TCGTATGGGA TCTCCTGCTC TCACCACTCG AGGATTTTTG GAGAAGGATT TTGCACAAGT AGCTCAGTTT GTGCATGAAG GTGTTCAAAT TGCTATCAAG ACCAAGAGTG AATTGGATCA AAAAGGTTTG AAGAAAGTGA AGGACTTTAA GGATTATTTG GATCAAAATC CAGATTCTGT TGGAATTACT GCATTGAAGA GTAAGGTTGA GACCTTTAGC AAATCATTCC CAACCATTGG TTTTGATAAG GATTCAATGA AATACAAGTG AGTAAGATAG GATCCGGCTG CTAACAAAGC CCGAAAGGAA GCTGAGTTGG CTGCTGCCAC CGCTGAGCAA TAACTAGCAT AACCCCTTGG GGCCTCTAAA CGGGTCTTGA GGGGTTTTTT GCTGAAAGGA GGAACTATAT CCGGATATCC ACAGGACGGG TGTGGTCGCC ATGATCGCGT AGTCGATAGT GGCTCCAAGT AGCGAAGCGA GCAGGACTGG GCGGCGGCCA AAGCGGTCGG ACAGTGCTCC GAGAACGGGT GCGCATAGAA ATTGCATCAA CGCATATAGC GCTAGCAGCA CGCCATAGTG ACTGGCGATG CTGTCGGAAT GGACGATATC CCGCAAGAGG CCCGGCAGTA CCGGCATAAC CAAGCCTATG CCTACAGCAT CCAGGGTGAC GGTGCCGAGG ATGACGATGA GCGCATTGTT AGATTTCATA CACGGTGCCT GACTGCGTTA GCAATTTAAC TGTGATAAAC TACCGCATTA AAGCTTATCG ATGATAAGCT GTCAAACATG AGAATTCTTG AAGACGAAAG GGCCTCGTGA TACGCCTATT TTTATAGGTT AATGTCATGA TAATAATGGT TTCTTAGACG TCAGGTGGCA CTTTTCGGGG AAATGTGCGC GGAACCCCTA TTTGTTTATT TTTCTAAATA CATTCAAATA TGTATCCGCT CATGAGACAA TAACCCTGAT AAATGCTTCA ATAATATTGA AAAAGGAAGA GTATGAGTAT TCAACATTTC CGTGTCGCCC TTATTCCCTT TTTTGCGGCA TTTTGCCTTC CTGTTTTTGC TCACCCAGAA ACGCTGGTGA AAGTAAAAGA TGCTGAAGAT CAGTTGGGTG CACGAGTGGG TTACATCGAA CTGGATCTCA ACAGCGGTAA GATCCTTGAG AGTTTTCGCC CCGAAGAACG TTTTCCAATG ATGAGCACTT TTAAAGTTCT GCTATGTGGC GCGGTATTAT CCCGTGTTGA CGCCGGGCAA GAGCAACTCG GTCGCCGCAT ACACTATTCT CAGAATGACT TGGTTGAGTA CTCACCAGTC ACAGAAAAGC ATCTTACGGA TGGCATGACA GTAAGAGAAT TATGCAGTGC TGCCATAACC ATGAGTGATA ACACTGCGGC CAACTTACTT CTGACAACGA TCGGAGGACC GAAGGAGCTA ACCGCTTTTT TGCACAACAT GGGGGATCAT GTAACTCGCC TTGATCGTTG GGAACCGGAG CTGAATGAAG CCATACCAAA CGACGAGCGT GACACCACGA TGCCTGCAGC AATGGCAACA ACGTTGCGCA AACTATTAAC TGGCGAACTA CTTACTCTAG CTTCCCGGCA ACAATTAATA GACTGGATGG AGGCGGATAA AGTTGCAGGA CCACTTCTGC GCTCGGCCCT TCCGGCTGGC TGGTTTATTG CTGATAAATC TGGAGCCGGT GAGCGTGGGT CTCGCGGTAT CATTGCAGCA CTGGGGCCAG ATGGTAAGCC CTCCCGTATC GTAGTTATCT ACACGACGGG GAGTCAGGCA ACTATGGATG AACGAAATAG ACAGATCGCT GAGATAGGTG CCTCACTGAT TAAGCATTGG TAACTGTCAG ACCAAGTTTA CTCATATATA CTTTAGATTG ATTTAAAACT TCATTTTTAA TTTAAAAGGA TCTAGGTGAA GATCCTTTTT GATAATCTCA TGACCAAAAT CCCTTAACGT GAGTTTTCGT TCCACTGAGC GTCAGACCCC GTAGAAAAGA TCAAAGGATC TTCTTGAGAT CCTTTTTTTC TGCGCGTAAT CTGCTGCTTG CAAACAAAAA AACCACCGCT ACCAGCGGTG GTTTGTTTGC CGGATCAAGA GCTACCAACT CTTTTTCCGA AGGTAACTGG CTTCAGCAGA GCGCAGATAC CAAATACTGT CCTTCTAGTG TAGCCGTAGT TAGGCCACCA CTTCAAGAAC TCTGTAGCAC CGCCTACATA CCTCGCTCTG CTAATCCTGT TACCAGTGGC TGCTGCCAGT GGCGATAAGT CGTGTCTTAC CGGGTTGGAC TCAAGACGAT AGTTACCGGA TAAGGCGCAG CGGTCGGGCT GAACGGGGGG TTCGTGCACA CAGCCCAGCT TGGAGCGAAC GACCTACACC GAACTGAGAT ACCTACAGCG TGAGCTATGA GAAAGCGCCA CGCTTCCCGA AGGGAGAAAG GCGGACAGGT ATCCGGTAAG CGGCAGGGTC GGAACAGGAG AGCGCACGAG GGAGCTTCCA GGGGGAAACG CCTGGTATCT TTATAGTCCT GTCGGGTTTC GCCACCTCTG ACTTGAGCGT CGATTTTTGT GATGCTCGTC AGGGGGGCGG AGCCTATGGA AAAACGCCAG CAACGCGGCC TTTTTACGGT TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCT GATGCGGTAT TTTCTCCTTA CGCATCTGTG CGGTATTTCA CACCGCATAT ATGGTGCACT CTCAGTACAA TCTGCTCTGA TGCCGCATAG TTAAGCCAGT ATACACTCCG CTATCGCTAC GTGACTGGGT CATGGCTGCG CCCCGACACC CGCCAACACC CGCTGACGCG CCCTGACGGG CTTGTCTGCT CCCGGCATCC GCTTACAGAC AAGCTGTGAC CGTCTCCGGG AGCTGCATGT GTCAGAGGTT TTCACCGTCA TCACCGAAAC GCGCGAGGCA GCTGCGGTAA AGCTCATCAG CGTGGTCGTG AAGCGATTCA CAGATGTCTG CCTGTTCATC CGCGTCCAGC TCGTTGAGTT TCTCCAGAAG CGTTAATGTC TGGCTTCTGA TAAAGCGGGC CATGTTAAGG GCGGTTTTTT CCTGTTTGGT CACTGATGCC TCCGTGTAAG GGGGATTTCT GTTCATGGGG GTAATGATAC CGATGAAACG AGAGAGGATG CTCACGATAC GGGTTACTGA TGATGAACAT GCCCGGTTAC TGGAACGTTG TGAGGGTAAA CAACTGGCGG TATGGATGCG GCGGGACCAG AGAAAAATCA CTCAGGGTCA ATGCCAGCGC TTCGTTAATA CAGATGTAGG TGTTCCACAG GGTAGCCAGC AGCATCCTGC GATGCAGATC CGGAACATAA TGGTGCAGGG CGCTGACTTC CGCGTTTCCA GACTTTACGA AACACGGAAA CCGAAGACCA TTCATGTTGT TGCTCAGGTC GCAGACGTTT TGCAGCAGCA GTCGCTTCAC GTTCGCTCGC GTATCGGTGA TTCATTCTGC TAACCAGTAA GGCAACCCCG CCAGCCTAGC CGGGTCCTCA ACGACAGGAG CACGATCATG CGCACCCGTG GCCAGGACCC AACGCTGCCC GAGATGCGCC GCGTGCGGCT GCTGGAGATG GCGGACGCGA TGGATATGTT CTGCCAAGGG TTGGTTTGCG CATTCACAGT TCTCCGCAAG AATTGATTGG CTCCAATTCT TGGAGTGGTG AATCCGTTAG CGAGGTGCCG CCGGCTTCCA TTCAGGTCGA GGTGGCCCGG CTCCATGCAC CGCGACGCAA CGCGGGGAGG CAGACAAGGT ATAGGGCGGC GCCTACAATC CATGCCAACC CGTTCCATGT GCTCGCCGAG GCGGCATAAA TCGCCGTGAC GATCAGCGGT CCAGTGATCG AAGTTAGGCT GGTAAGAGCC GCGAGCGATC CTTGAAGCTG TCCCTGATGG TCGTCATCTA CCTGCCTGGA CAGCATGGCC TGCAACGCGG GCATCCCGAT GCCGCCGGAA GCGAGAAGAA TCATAATGGG GAAGGCCATC CAGCCTCGCG TCGCGAACGC CAGCAAGACG TAGCCCAGCG CGTCGGCCGC CATGCCGGCG ATAATGGCCT GCTTCTCGCC GAAACGTTTG GTGGCGGGAC CAGTGACGAA GGCTTGAGCG AGGGCGTGCA AGATTCCGAA TACCGCAAGC GACAGGCCGA TCATCGTCGC GCTCCAGCGA AAGCGGTCCT CGCCGAAAAT GACCCAGAGC GCTGCCGGCA CCTGTCCTAC GAGTTGCATG ATAAAGAAGA CAGTCATAAG TGCGGCGACG ATAGTCATGC CCCGCGCCCA CCGGAAGGAG CTGACTGGGT TGAAGGCTCT CAAGGGCATC GGTCGACGCT CTCCCTTATG CGACTCCTGC ATTAGGAAGC AGCCCAGTAG TAGGTTGAGG CCGTTGAGCA CCGCCGCCGC AAGGAATGGT GCATGCAAGG AGATGGCGCC CAACAGTCCC CCGGCCACGG GGCCTGCCAC CATACCCACG CCGAAACAAG CGCTCATGAG CCCGAAGTGG CGAGCCCGAT CTTCCCCATC GGTGATGTCG GCGATATAGG CGCCAGCAAC CGCACCTGTG GCGCCGGTGA TGCCGGCCAC GATGCGTCCG GCGTAGAGGA TCGAGATCTC GATCCCGCGA AAT