NafoA.00005.b

malate dehydrogenase (cytoplasmic) (MDH1)

CENTER ID:	NafoA.00005.b
ORGANISM:	Naegleria fowleri ATCC 30863
ASSOCIATED DISEASE:
CURRENT STATUS:	cloned
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIB

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
NafoA.00005.b.B1.GE40136	Full length(NafoA.00005.b)		1	330

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
EuPathDB:	AmoebaDB:NF0114780

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MTKQTLKVLI TGAAGQIGYS LIGMVASGLM LGFDQPIHLH LLEVPQVLDA LKGVCMELTD SAYPLLQKVV ATSDYQEAFS DIDICIMVGA MPRKEGMERK DLLEKNAAIF KGQGEALEKW AKKTVKVLVV GNPANTNCLI AKTHAPSIPA ENFSALTRLD HNRAKAQVAE RLGINVSDVH NVIIWGNHSS TQFPDVAHGY VTINGQKKSI YEAINDDAWL KGDFISTVQK RGAAVIAARK LSSATSAAKA ITDHMHDWIL GTPEGEWVSM AIPSDGSYGI PEGVIFSFPV TCKNGTVSIV QGLKIDDFAR EKLNITDKEL REEKSMALSI

NT Sequence

ATGACCAAGC AAACATTGAA GGTTTTGATT ACTGGTGCTG CTGGACAAAT CGGATACAGC TTGATTGGTA TGGTTGCTAG CGGATTAATG CTCGGTTTTG ATCAGCCAAT ACACCTTCAC TTGCTTGAAG TACCACAAGT TTTGGATGCT TTGAAGGGAG TTTGTATGGA ACTTACCGAT TCAGCTTATC CTTTGCTCCA AAAGGTGGTT GCCACTAGTG ATTATCAAGA AGCCTTCAGT GATATTGATA TTTGTATCAT GGTCGGTGCC ATGCCAAGAA AAGAAGGTAT GGAGAGAAAG GATTTGTTAG AGAAGAACGC TGCAATTTTC AAGGGACAAG GTGAAGCTTT GGAAAAGTGG GCCAAGAAGA CTGTCAAGGT CCTTGTTGTT GGTAACCCAG CCAATACCAA TTGTCTCATT GCCAAGACTC ATGCACCTTC AATTCCTGCT GAAAACTTCT CTGCTCTCAC TCGTTTGGAT CATAACAGAG CAAAGGCTCA AGTTGCTGAG CGTCTCGGTA TCAACGTTTC TGATGTTCAT AATGTCATTA TTTGGGGAAA TCACTCATCA ACTCAATTCC CTGATGTTGC TCATGGTTAT GTCACAATTA ATGGACAGAA AAAGTCTATT TATGAAGCCA TCAATGACGA CGCTTGGTTG AAGGGAGATT TCATTTCTAC TGTTCAAAAG AGAGGAGCTG CAGTTATTGC TGCAAGAAAG CTTTCTTCTG CTACATCAGC TGCTAAGGCA ATCACTGATC ACATGCATGA TTGGATTTTG GGTACACCTG AGGGTGAATG GGTCAGTATG GCCATCCCTT CTGATGGATC TTATGGCATT CCAGAAGGTG TAATTTTCTC TTTCCCAGTT ACTTGCAAGA ATGGAACCGT CTCCATTGTT CAAGGTTTGA AGATTGATGA TTTTGCCAGA GAAAAGCTCA ACATTACTGA CAAGGAGTTG AGAGAAGAAA AATCAATGGC TTTGTCAATT

Details for NafoA.00005.b.B1.GE40136

HARVESTED ON:	data unavailable
SEQUENCED ON:	4/13/2016
EXPECTED MW:	37kDa
OBSERVED MW:	60kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Few (1-10)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	High Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY:	99
PERCENT COVERAGE:	97

Validated AA Sequence

MAHHHHHHMT KQTLKVLITG AXXQIGYSLI GMVASGLMLG FDQPIHLHLL EVPQVLDALK GVCMELTDSA YPLLQKVVAT SDYQEAFSDI DICIMVGAMP RKEGMERKDL LEKNAAIFKG QGEALEKWAK KTVKVLVVGN PANTNCLIAK THAPSIPAEN FSALTRLDHN RAKAQVAERL GINVSDVHNV IIWGNHSSTQ FPDVAHGYVT INGQKKSIYE AINDDAWLKG DFISTVQKRG AAVIAARKLS SATSAAKAIT DHMHDWILGT PEGEWVSMAI PSDGSYGIPE GVIFSFPVTC KNGTVSIVQG LKIDDFAREK LNITDKELRE

Validated NT Sequence

tncttctctc aactccttgt cagtaatgtt gagcttttct ctggcaaaat catcaatctt caaaccttga acaatggaga cggttccatt cttgcaagta actgggaaag agaaaattac accttctgga atgccataag atccatcaga agggatggcc atactgaccc attcaccctc aggtgtaccc aaaatccaat catgcatgtg atcagtgatt gccttagcag ctgatgtagc agaagaaagc tttcttgcag caataactgc agctcctctc ttttgaacag tagaaatgaa atctcccttc aaccaagcgt cgtcattgat ggcttcataa atagactttt tctgtccatt aattgtgaca taaccatgag caacatcagg gaattgagtt gatgagtgat ttccccaaat aatgacatta tgaacatcag aaacgttgat accgagacgc tcagcaactt gagcctttgc tctgttatga tccaaacgag tgagagcaga gaagttttca gcaggaattg aaggtgcatg agtcttggca atgagacaat tggtattggc tgggttacca acaacaagga ccttgacagt cttcttggcc cacttttcca aagcttcacc ttgtcccttg aaaattgcag cgttcttctc taacaaatcc tttctctcca taccttcttt tcttggcatg gcaccgacca tgatacaaat atcaatatca ctgaaggctt cttgataatc actagtggca accacctttt ggagcaaagg ataagctgaa tcggtaagtt ccatacaaac tcccttcaaa gcatccaaaa cttgtggtac ttcaagcaag tgaaggtgta ttggctgatc aaaaccgagc attaatccgc tagcaaccat accaatcaag ctgtatccga tttgtnnnnc agcaccagta atcaaaacct tcaatgtttg cttggtcata tggtggtggt ggtggtgagc cat

Expected Protein Sequence

MAHHHHHHMT KQTLKVLITG AAGQIGYSLI GMVASGLMLG FDQPIHLHLL EVPQVLDALK GVCMELTDSA YPLLQKVVAT SDYQEAFSDI DICIMVGAMP RKEGMERKDL LEKNAAIFKG QGEALEKWAK KTVKVLVVGN PANTNCLIAK THAPSIPAEN FSALTRLDHN RAKAQVAERL GINVSDVHNV IIWGNHSSTQ FPDVAHGYVT INGQKKSIYE AINDDAWLKG DFISTVQKRG AAVIAARKLS SATSAAKAIT DHMHDWILGT PEGEWVSMAI PSDGSYGIPE GVIFSFPVTC KNGTVSIVQG LKIDDFAREK LNITDKELRE EKSMALSI

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgacca agcaaacatt gaaggttttg attactggtg ctgctggaca aatcggatac agcttgattg gtatggttgc tagcggatta atgctcggtt ttgatcagcc aatacacctt cacttgcttg aagtaccaca agttttggat gctttgaagg gagtttgtat ggaacttacc gattcagctt atcctttgct ccaaaaggtg gttgccacta gtgattatca agaagccttc agtgatattg atatttgtat catggtcggt gccatgccaa gaaaagaagg tatggagaga aaggatttgt tagagaagaa cgctgcaatt ttcaagggac aaggtgaagc tttggaaaag tgggccaaga agactgtcaa ggtccttgtt gttggtaacc cagccaatac caattgtctc attgccaaga ctcatgcacc ttcaattcct gctgaaaact tctctgctct cactcgtttg gatcataaca gagcaaaggc tcaagttgct gagcgtctcg gtatcaacgt ttctgatgtt cataatgtca ttatttgggg aaatcactca tcaactcaat tccctgatgt tgctcatggt tatgtcacaa ttaatggaca gaaaaagtct atttatgaag ccatcaatga cgacgcttgg ttgaagggag atttcatttc tactgttcaa aagagaggag ctgcagttat tgctgcaaga aagctttctt ctgctacatc agctgctaag gcaatcactg atcacatgca tgattggatt ttgggtacac ctgagggtga atgggtcagt atggccatcc cttctgatgg atcttatggc attccagaag gtgtaatttt ctctttccca gttacttgca agaatggaac cgtctccatt gttcaaggtt tgaagattga tgattttgcc agagaaaagc tcaacattac tgacaaggag ttgagagaag aaaaatcaat ggctttgtca atttgagtaa gataggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg gcatcggtcg acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta gaggatcgag atctcgatcc cgcgaaat