NafoA.00005.a
malate dehydrogenase (mitochondrial) (MDH2)
| CENTER ID: | NafoA.00005.a |
| ORGANISM: | Naegleria fowleri ATCC 30863 |
| ASSOCIATED DISEASE: | |
| CURRENT STATUS: | in PDB |
| COMMUNITY REQUEST: | True |
| NIH RISK GROUP: | 2 |
| SELECT AGENT: | False |
| NIH PRIORITY pathogens category: |
IIB |
Ordering Clones & Proteins
If there are materials available for this target, they will be listed below.
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Clones*
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| NafoA.00005.a.B1.GE40135 | Full length(NafoA.00005.a) | 1 | 436 |
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information
using the button in the "info" column.
Proteins
| CENTER REFERENCE ID |
DOMAIN/REGION DESCRIPTION |
INFO | AA START |
AA STOP |
ORDER MATERIAL |
|---|---|---|---|---|---|
| NafoA.00005.a.B1.PS38166 | Full length(NafoA.00005.a) | 1 | 436 |
Structures
6UM4
DEPOSITED: 10/9/2019
DETERMINATION: XRay
CLONE: NafoA.00005.a.B1.GE40135
PROTEIN: NafoA.00005.a.B1.PS38166
External Resources
| RESOURCE | REFERENCE ID |
|---|---|
| EuPathDB: | AmoebaDB:NF0021050 |
Sequences
These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols.
To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKRLVPQFSK SSRNGVRHYS TLKQRGEKMD AELSATRTPL ERMLLGKQTK NDFANLNIKD PKTVAQWLNK SAREGPKYLT KDVDQLAPPV TITVSGACGQ IANSLLFRIA NGEMLGENQP VKLRLLERPE AMKALEGVKM ELDDGAFPLL EEIYLTDSEN DAFRGADYAI LLGGKPRGPG MERADVMKDN AAIFKAQGEA LNKQANGDVL VLIVANPANT NAMITSANAP DIPPENITAM TRLDHDRGLA QVAAKVGCNI TDISRFAIWG NHSATQYPDL SFTTIKGQWG LNVINDEQWI TNEFIPNVQQ RGAAIIKARG KSSAASAADA AIKHMHDWVL GNSEWVSMAI PSRGQYGIPR GIWCSMPVQC FGAGKYGVIE GLPINSFSAD RINASVKELI EEKKIVENLL PNPVYRLVEV DKVNIISKSY ISENKQ
NT Sequence
ATGAAGAGAC TCGTCCCCCA ATTCTCCAAA TCTTCACGTA ATGGTGTCCG CCATTATAGC ACCTTGAAAC AAAGAGGTGA AAAGATGGAT GCCGAATTGT CTGCAACAAG AACACCACTT GAAAGAATGT TGCTTGGAAA GCAAACCAAG AACGATTTTG CCAATTTGAA CATTAAAGAT CCAAAGACAG TCGCTCAATG GTTGAACAAG AGTGCTAGAG AGGGACCAAA ATACTTGACA AAGGATGTCG ATCAATTGGC TCCTCCAGTT ACAATCACTG TCTCTGGAGC ATGTGGACAA ATTGCCAATA GCCTGTTGTT CAGAATTGCT AACGGTGAAA TGTTGGGCGA AAATCAACCA GTGAAGTTGA GATTGTTGGA AAGACCAGAA GCAATGAAGG CTCTTGAAGG AGTGAAAATG GAATTGGACG ATGGCGCTTT CCCACTCTTG GAAGAAATTT ATCTTACCGA TAGTGAAAAT GATGCTTTCA GAGGTGCTGA CTATGCTATT TTGCTCGGAG GAAAGCCAAG AGGTCCTGGA ATGGAAAGAG CTGATGTCAT GAAAGACAAT GCTGCTATTT TTAAGGCTCA AGGTGAGGCT TTGAACAAGC AAGCTAACGG AGATGTTTTG GTTTTGATTG TTGCCAATCC AGCCAACACT AACGCTATGA TTACATCTGC CAATGCTCCA GATATTCCAC CAGAAAATAT CACTGCAATG ACAAGACTTG ATCACGATAG AGGATTGGCT CAAGTTGCAG CAAAGGTTGG ATGTAATATT ACCGACATTT CACGATTTGC TATTTGGGGT AACCACTCTG CCACTCAATA TCCAGATCTT TCATTCACAA CCATCAAAGG ACAATGGGGT CTTAACGTGA TTAATGACGA ACAATGGATT ACAAATGAAT TTATTCCAAA CGTGCAACAA CGTGGTGCTG CTATCATCAA GGCCAGAGGA AAGTCAAGTG CTGCTTCTGC TGCCGACGCT GCTATTAAAC ATATGCACGA TTGGGTTTTG GGAAATAGCG AATGGGTTTC TATGGCTATT CCATCAAGAG GTCAATATGG TATCCCAAGA GGTATTTGGT GTTCAATGCC AGTGCAATGC TTTGGAGCTG GTAAATACGG AGTCATTGAA GGTTTACCAA TCAACTCCTT CAGCGCTGAT AGAATTAATG CTAGCGTAAA GGAACTTATT GAGGAAAAGA AGATTGTTGA AAATTTATTA CCAAATCCAG TTTACAGATT AGTCGAAGTT GACAAGGTTA ACATTATTAG CAAGTCATAC ATCTCAGAAA ACAAGCAA
Details for NafoA.00005.a.B1.GE40135
| HARVESTED ON: | 4/6/2016 |
| SEQUENCED ON: | 4/13/2016 |
| EXPECTED MW: | 49kDa |
| OBSERVED MW: | 50kDa |
| ANTIBIOTIC MARKER: | ampicillin |
| COUNT OF EXPRESSION COLONIES: | Many (50-100) |
| TOTAL EXPRESSION LEVEL: | Moderate Expression |
| SOLUBLE EXPRESSION LEVEL | Moderate Expression |
| EXPRESSION HOST: | data unavailable |
| SEQUENCING RESULT: | |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 97 |
Validated AA Sequence
MAHHHHHMKR LVPQFSKSSR NXXRHYSTLK QRGEKMDAEL SATRTPLERM LLGKQTKNDF ANLNIKDPKT VAQWLNKSAR EGPKYLTKDV DQLAPPVTIT VSGACGQIAN SLLFRIANGE MLGENQPVKL RLLERPEAMK ALEGVKMELD DGAFPLLEEI YLTDSENDAF RGADYAILLG GKPRGPGMER ADVMKDNAAI FKAQGEALNK QANGDVLVLI VANPANTNAM ITSANAPDIP PENITAMTRL DHDRGLAQVA AKVGCNITDI SRFAIWGNHS ATQYPDLSFT TIKGQWGLNV INDEQWITNE FIPNVQQRGA AIIKARGKSS AASAADAAIK HMHDWVLGNS EWVSMAIPSR GQYGIPRGIW CSMPVQCFGA GKYGVIEGLP INSFSADRIN ASVKELIEEK KIVENLLPNP VYRLVEVDKV NIIS
Validated NT Sequence
cttgctaata atgttaacct tgtcaacttc gactaatctg taaactggat ttggtaataa attttcaaca atcttctttt cctcaataag ttcctttacg ctagcattaa ttctatcagc gctgaaggag ttgattggta aaccttcaat gactccgtat ttaccagctc caaagcattg cactggcatt gaacaccaaa tacctcttgg gataccatat tgacctcttg atggaatagc catagaaacc cattcgctat ttcccaaaac ccaatcgtgc atatgtttaa tagcagcgtc ggcagcagaa gcagcacttg actttcctct ggccttgatg atagcagcac cacgttgttg cacgtttgga ataaattcat ttgtaatcca ttgttcgtca ttaatcacgt taagacccca ttgtcctttg atggttgtga atgaaagatc tggatattga gtggcagagt ggttacccca aatagcaaat cgtgaaatgt cggtaatatt acatccaacc tttgctgcaa cttgagccaa tcctctatcg tgatcaagtc ttgtcattgc agtgatattt tctggtggaa tatctggagc attggcagat gtaatcatag cgttagtgtt ggctggattg gcaacaatca aaaccaaaac atctccgtta gcttgcttgt tcaaagcctc accttgagcc ttaaaaatag cagcattgtc tttcatgaca tcagctcttt ccattccagg acctcttggc tttcctccga gcaaaatagc atagtcagca cctctgaaag catcattttc actatcggta agataaattt cttccaagag tgggaaagcg ccatcgtcca attccatttt cactccttca agagccttca ttgcttctgg tctttccaac aatctcaact tcactggttg attttcgccc aacatttcac cgttagcaat tctgaacaac aggctattgg caatttgtcc acatgctcca gagacagtga ttgtaactgg aggagccaat tgatcgacat cctttgtcaa gtattttggt ccctctctag cactcttgtt caaccattga gcgactgtct ttggatcttt aatgttcaaa ttggcaaaat cgttcttggt ttgctttcca agcaacattc tttcaagtgg tgttcttgtt gcagacaatt cggcatccat cttttcacct ctttgtttca aggtgctata atggcggnnn ncattacgtg aagatttgga gaattggggg acgagtctct tcatatggtg gtggtggtga gccat
Expected Protein Sequence
MAHHHHHHMK RLVPQFSKSS RNGVRHYSTL KQRGEKMDAE LSATRTPLER MLLGKQTKND FANLNIKDPK TVAQWLNKSA REGPKYLTKD VDQLAPPVTI TVSGACGQIA NSLLFRIANG EMLGENQPVK LRLLERPEAM KALEGVKMEL DDGAFPLLEE IYLTDSENDA FRGADYAILL GGKPRGPGME RADVMKDNAA IFKAQGEALN KQANGDVLVL IVANPANTNA MITSANAPDI PPENITAMTR LDHDRGLAQV AAKVGCNITD ISRFAIWGNH SATQYPDLSF TTIKGQWGLN VINDEQWITN EFIPNVQQRG AAIIKARGKS SAASAADAAI KHMHDWVLGN SEWVSMAIPS RGQYGIPRGI WCSMPVQCFG AGKYGVIEGL PINSFSADRI NASVKELIEE KKIVENLLPN PVYRLVEVDK VNIISKSYIS ENKQ
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgaaga gactcgtccc ccaattctcc aaatcttcac gtaatggtgt ccgccattat agcaccttga aacaaagagg tgaaaagatg gatgccgaat tgtctgcaac aagaacacca cttgaaagaa tgttgcttgg aaagcaaacc aagaacgatt ttgccaattt gaacattaaa gatccaaaga cagtcgctca atggttgaac aagagtgcta gagagggacc aaaatacttg acaaaggatg tcgatcaatt ggctcctcca gttacaatca ctgtctctgg agcatgtgga caaattgcca atagcctgtt gttcagaatt gctaacggtg aaatgttggg cgaaaatcaa ccagtgaagt tgagattgtt ggaaagacca gaagcaatga aggctcttga aggagtgaaa atggaattgg acgatggcgc tttcccactc ttggaagaaa tttatcttac cgatagtgaa aatgatgctt tcagaggtgc tgactatgct attttgctcg gaggaaagcc aagaggtcct ggaatggaaa gagctgatgt catgaaagac aatgctgcta tttttaaggc tcaaggtgag gctttgaaca agcaagctaa cggagatgtt ttggttttga ttgttgccaa tccagccaac actaacgcta tgattacatc tgccaatgct ccagatattc caccagaaaa tatcactgca atgacaagac ttgatcacga tagaggattg gctcaagttg cagcaaaggt tggatgtaat attaccgaca tttcacgatt tgctatttgg ggtaaccact ctgccactca atatccagat ctttcattca caaccatcaa aggacaatgg ggtcttaacg tgattaatga cgaacaatgg attacaaatg aatttattcc aaacgtgcaa caacgtggtg ctgctatcat caaggccaga ggaaagtcaa gtgctgcttc tgctgccgac gctgctatta aacatatgca cgattgggtt ttgggaaata gcgaatgggt ttctatggct attccatcaa gaggtcaata tggtatccca agaggtattt ggtgttcaat gccagtgcaa tgctttggag ctggtaaata cggagtcatt gaaggtttac caatcaactc cttcagcgct gatagaatta atgctagcgt aaaggaactt attgaggaaa agaagattgt tgaaaattta ttaccaaatc cagtttacag attagtcgaa gttgacaagg ttaacattat tagcaagtca tacatctcag aaaacaagca atgagtaaga taggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagctgcgg taaagctcat cagcgtggtc gtgaagcgat tcacagatgt ctgcctgttc atccgcgtcc agctcgttga gtttctccag aagcgttaat gtctggcttc tgataaagcg ggccatgtta agggcggttt tttcctgttt ggtcactgat gcctccgtgt aagggggatt tctgttcatg ggggtaatga taccgatgaa acgagagagg atgctcacga tacgggttac tgatgatgaa catgcccggt tactggaacg ttgtgagggt aaacaactgg cggtatggat gcggcgggac cagagaaaaa tcactcaggg tcaatgccag cgcttcgtta atacagatgt aggtgttcca cagggtagcc agcagcatcc tgcgatgcag atccggaaca taatggtgca gggcgctgac ttccgcgttt ccagacttta cgaaacacgg aaaccgaaga ccattcatgt tgttgctcag gtcgcagacg ttttgcagca gcagtcgctt cacgttcgct cgcgtatcgg tgattcattc tgctaaccag taaggcaacc ccgccagcct agccgggtcc tcaacgacag gagcacgatc atgcgcaccc gtggccagga cccaacgctg cccgagatgc gccgcgtgcg gctgctggag atggcggacg cgatggatat gttctgccaa gggttggttt gcgcattcac agttctccgc aagaattgat tggctccaat tcttggagtg gtgaatccgt tagcgaggtg ccgccggctt ccattcaggt cgaggtggcc cggctccatg caccgcgacg caacgcgggg aggcagacaa ggtatagggc ggcgcctaca atccatgcca acccgttcca tgtgctcgcc gaggcggcat aaatcgccgt gacgatcagc ggtccagtga tcgaagttag gctggtaaga gccgcgagcg atccttgaag ctgtccctga tggtcgtcat ctacctgcct ggacagcatg gcctgcaacg cgggcatccc gatgccgccg gaagcgagaa gaatcataat ggggaaggcc atccagcctc gcgtcgcgaa cgccagcaag acgtagccca gcgcgtcggc cgccatgccg gcgataatgg cctgcttctc gccgaaacgt ttggtggcgg gaccagtgac gaaggcttga gcgagggcgt gcaagattcc gaataccgca agcgacaggc cgatcatcgt cgcgctccag cgaaagcggt cctcgccgaa aatgacccag agcgctgccg gcacctgtcc tacgagttgc atgataaaga agacagtcat aagtgcggcg acgatagtca tgccccgcgc ccaccggaag gagctgactg ggttgaaggc tctcaagggc atcggtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaat
Details for NafoA.00005.a.B1.PS38166
| PURIFICATION DATe: | 1/12/2017 |
| CONCENTRATION: | 16.9mg/ml |
| OBSERVED MW: | 37kDa |
| EXPRESSION LEVEL: | Moderate Expression |
| PROTEIN PURIFICATION BUFFER: | SEC |
| EXPRESSION HOST: | data unavailable |
| VIAL COUNT (approx.): | 4 |
| VIAL VOLUME: | 200µl |
| PERCENT IDENTITY: | 99 |
| PERCENT COVERAGE: | 97 |
Protocol Notes
notes unavailable
Validated AA Sequence
MAHHHHHMKR LVPQFSKSSR NXXRHYSTLK QRGEKMDAEL SATRTPLERM LLGKQTKNDF ANLNIKDPKT VAQWLNKSAR EGPKYLTKDV DQLAPPVTIT VSGACGQIAN SLLFRIANGE MLGENQPVKL RLLERPEAMK ALEGVKMELD DGAFPLLEEI YLTDSENDAF RGADYAILLG GKPRGPGMER ADVMKDNAAI FKAQGEALNK QANGDVLVLI VANPANTNAM ITSANAPDIP PENITAMTRL DHDRGLAQVA AKVGCNITDI SRFAIWGNHS ATQYPDLSFT TIKGQWGLNV INDEQWITNE FIPNVQQRGA AIIKARGKSS AASAADAAIK HMHDWVLGNS EWVSMAIPSR GQYGIPRGIW CSMPVQCFGA GKYGVIEGLP INSFSADRIN ASVKELIEEK KIVENLLPNP VYRLVEVDKV NIIS
Validated NT Sequence
cttgctaata atgttaacct tgtcaacttc gactaatctg taaactggat ttggtaataa attttcaaca atcttctttt cctcaataag ttcctttacg ctagcattaa ttctatcagc gctgaaggag ttgattggta aaccttcaat gactccgtat ttaccagctc caaagcattg cactggcatt gaacaccaaa tacctcttgg gataccatat tgacctcttg atggaatagc catagaaacc cattcgctat ttcccaaaac ccaatcgtgc atatgtttaa tagcagcgtc ggcagcagaa gcagcacttg actttcctct ggccttgatg atagcagcac cacgttgttg cacgtttgga ataaattcat ttgtaatcca ttgttcgtca ttaatcacgt taagacccca ttgtcctttg atggttgtga atgaaagatc tggatattga gtggcagagt ggttacccca aatagcaaat cgtgaaatgt cggtaatatt acatccaacc tttgctgcaa cttgagccaa tcctctatcg tgatcaagtc ttgtcattgc agtgatattt tctggtggaa tatctggagc attggcagat gtaatcatag cgttagtgtt ggctggattg gcaacaatca aaaccaaaac atctccgtta gcttgcttgt tcaaagcctc accttgagcc ttaaaaatag cagcattgtc tttcatgaca tcagctcttt ccattccagg acctcttggc tttcctccga gcaaaatagc atagtcagca cctctgaaag catcattttc actatcggta agataaattt cttccaagag tgggaaagcg ccatcgtcca attccatttt cactccttca agagccttca ttgcttctgg tctttccaac aatctcaact tcactggttg attttcgccc aacatttcac cgttagcaat tctgaacaac aggctattgg caatttgtcc acatgctcca gagacagtga ttgtaactgg aggagccaat tgatcgacat cctttgtcaa gtattttggt ccctctctag cactcttgtt caaccattga gcgactgtct ttggatcttt aatgttcaaa ttggcaaaat cgttcttggt ttgctttcca agcaacattc tttcaagtgg tgttcttgtt gcagacaatt cggcatccat cttttcacct ctttgtttca aggtgctata atggcggnnn ncattacgtg aagatttgga gaattggggg acgagtctct tcatatggtg gtggtggtga gccat
Expressed Protein Sequence
MAHHHHHHMK RLVPQFSKSS RNGVRHYSTL KQRGEKMDAE LSATRTPLER MLLGKQTKND FANLNIKDPK TVAQWLNKSA REGPKYLTKD VDQLAPPVTI TVSGACGQIA NSLLFRIANG EMLGENQPVK LRLLERPEAM KALEGVKMEL DDGAFPLLEE IYLTDSENDA FRGADYAILL GGKPRGPGME RADVMKDNAA IFKAQGEALN KQANGDVLVL IVANPANTNA MITSANAPDI PPENITAMTR LDHDRGLAQV AAKVGCNITD ISRFAIWGNH SATQYPDLSF TTIKGQWGLN VINDEQWITN EFIPNVQQRG AAIIKARGKS SAASAADAAI KHMHDWVLGN SEWVSMAIPS RGQYGIPRGI WCSMPVQCFG AGKYGVIEGL PINSFSADRI NASVKELIEE KKIVENLLPN PVYRLVEVDK VNIISKSYIS ENKQ
Full NT Sequence (Expression Vector + Insert)
TAATACGACT CACTATAGGG AGACCACAAC GGTTTCCCTC TAGAAATAAT TTTGTTTAAC TTTAAGAAGG AGATATACCA TGGCTCACCA CCACCACCAC CATATGAAGA GACTCGTCCC CCAATTCTCC AAATCTTCAC GTAATGGTGT CCGCCATTAT AGCACCTTGA AACAAAGAGG TGAAAAGATG GATGCCGAAT TGTCTGCAAC AAGAACACCA CTTGAAAGAA TGTTGCTTGG AAAGCAAACC AAGAACGATT TTGCCAATTT GAACATTAAA GATCCAAAGA CAGTCGCTCA ATGGTTGAAC AAGAGTGCTA GAGAGGGACC AAAATACTTG ACAAAGGATG TCGATCAATT GGCTCCTCCA GTTACAATCA CTGTCTCTGG AGCATGTGGA CAAATTGCCA ATAGCCTGTT GTTCAGAATT GCTAACGGTG AAATGTTGGG CGAAAATCAA CCAGTGAAGT TGAGATTGTT GGAAAGACCA GAAGCAATGA AGGCTCTTGA AGGAGTGAAA ATGGAATTGG ACGATGGCGC TTTCCCACTC TTGGAAGAAA TTTATCTTAC CGATAGTGAA AATGATGCTT TCAGAGGTGC TGACTATGCT ATTTTGCTCG GAGGAAAGCC AAGAGGTCCT GGAATGGAAA GAGCTGATGT CATGAAAGAC AATGCTGCTA TTTTTAAGGC TCAAGGTGAG GCTTTGAACA AGCAAGCTAA CGGAGATGTT TTGGTTTTGA TTGTTGCCAA TCCAGCCAAC ACTAACGCTA TGATTACATC TGCCAATGCT CCAGATATTC CACCAGAAAA TATCACTGCA ATGACAAGAC TTGATCACGA TAGAGGATTG GCTCAAGTTG CAGCAAAGGT TGGATGTAAT ATTACCGACA TTTCACGATT TGCTATTTGG GGTAACCACT CTGCCACTCA ATATCCAGAT CTTTCATTCA CAACCATCAA AGGACAATGG GGTCTTAACG TGATTAATGA CGAACAATGG ATTACAAATG AATTTATTCC AAACGTGCAA CAACGTGGTG CTGCTATCAT CAAGGCCAGA GGAAAGTCAA GTGCTGCTTC TGCTGCCGAC GCTGCTATTA AACATATGCA CGATTGGGTT TTGGGAAATA GCGAATGGGT TTCTATGGCT ATTCCATCAA GAGGTCAATA TGGTATCCCA AGAGGTATTT GGTGTTCAAT GCCAGTGCAA TGCTTTGGAG CTGGTAAATA CGGAGTCATT GAAGGTTTAC CAATCAACTC CTTCAGCGCT GATAGAATTA ATGCTAGCGT AAAGGAACTT ATTGAGGAAA AGAAGATTGT TGAAAATTTA TTACCAAATC CAGTTTACAG ATTAGTCGAA GTTGACAAGG TTAACATTAT TAGCAAGTCA TACATCTCAG AAAACAAGCA ATGAGTAAGA TAGGATCCGG CTGCTAACAA AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGATA TCCACAGGAC GGGTGTGGTC GCCATGATCG CGTAGTCGAT AGTGGCTCCA AGTAGCGAAG CGAGCAGGAC TGGGCGGCGG CCAAAGCGGT CGGACAGTGC TCCGAGAACG GGTGCGCATA GAAATTGCAT CAACGCATAT AGCGCTAGCA GCACGCCATA GTGACTGGCG ATGCTGTCGG AATGGACGAT ATCCCGCAAG AGGCCCGGCA GTACCGGCAT AACCAAGCCT ATGCCTACAG CATCCAGGGT GACGGTGCCG AGGATGACGA TGAGCGCATT GTTAGATTTC ATACACGGTG CCTGACTGCG TTAGCAATTT AACTGTGATA AACTACCGCA TTAAAGCTTA TCGATGATAA GCTGTCAAAC ATGAGAATTC TTGAAGACGA AAGGGCCTCG TGATACGCCT ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTGT TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGC AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCTA TGAGAAAGCG CCACGCTTCC CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCTGATGCGG TATTTTCTCC TTACGCATCT GTGCGGTATT TCACACCGCA TATATGGTGC ACTCTCAGTA CAATCTGCTC TGATGCCGCA TAGTTAAGCC AGTATACACT CCGCTATCGC TACGTGACTG GGTCATGGCT GCGCCCCGAC ACCCGCCAAC ACCCGCTGAC GCGCCCTGAC GGGCTTGTCT GCTCCCGGCA TCCGCTTACA GACAAGCTGT GACCGTCTCC GGGAGCTGCA TGTGTCAGAG GTTTTCACCG TCATCACCGA AACGCGCGAG GCAGCTGCGG TAAAGCTCAT CAGCGTGGTC GTGAAGCGAT TCACAGATGT CTGCCTGTTC ATCCGCGTCC AGCTCGTTGA GTTTCTCCAG AAGCGTTAAT GTCTGGCTTC TGATAAAGCG GGCCATGTTA AGGGCGGTTT TTTCCTGTTT GGTCACTGAT GCCTCCGTGT AAGGGGGATT TCTGTTCATG GGGGTAATGA TACCGATGAA ACGAGAGAGG ATGCTCACGA TACGGGTTAC TGATGATGAA CATGCCCGGT TACTGGAACG TTGTGAGGGT AAACAACTGG CGGTATGGAT GCGGCGGGAC CAGAGAAAAA TCACTCAGGG TCAATGCCAG CGCTTCGTTA ATACAGATGT AGGTGTTCCA CAGGGTAGCC AGCAGCATCC TGCGATGCAG ATCCGGAACA TAATGGTGCA GGGCGCTGAC TTCCGCGTTT CCAGACTTTA CGAAACACGG AAACCGAAGA CCATTCATGT TGTTGCTCAG GTCGCAGACG TTTTGCAGCA GCAGTCGCTT CACGTTCGCT CGCGTATCGG TGATTCATTC TGCTAACCAG TAAGGCAACC CCGCCAGCCT AGCCGGGTCC TCAACGACAG GAGCACGATC ATGCGCACCC GTGGCCAGGA CCCAACGCTG CCCGAGATGC GCCGCGTGCG GCTGCTGGAG ATGGCGGACG CGATGGATAT GTTCTGCCAA GGGTTGGTTT GCGCATTCAC AGTTCTCCGC AAGAATTGAT TGGCTCCAAT TCTTGGAGTG GTGAATCCGT TAGCGAGGTG CCGCCGGCTT CCATTCAGGT CGAGGTGGCC CGGCTCCATG CACCGCGACG CAACGCGGGG AGGCAGACAA GGTATAGGGC GGCGCCTACA ATCCATGCCA ACCCGTTCCA TGTGCTCGCC GAGGCGGCAT AAATCGCCGT GACGATCAGC GGTCCAGTGA TCGAAGTTAG GCTGGTAAGA GCCGCGAGCG ATCCTTGAAG CTGTCCCTGA TGGTCGTCAT CTACCTGCCT GGACAGCATG GCCTGCAACG CGGGCATCCC GATGCCGCCG GAAGCGAGAA GAATCATAAT GGGGAAGGCC ATCCAGCCTC GCGTCGCGAA CGCCAGCAAG ACGTAGCCCA GCGCGTCGGC CGCCATGCCG GCGATAATGG CCTGCTTCTC GCCGAAACGT TTGGTGGCGG GACCAGTGAC GAAGGCTTGA GCGAGGGCGT GCAAGATTCC GAATACCGCA AGCGACAGGC CGATCATCGT CGCGCTCCAG CGAAAGCGGT CCTCGCCGAA AATGACCCAG AGCGCTGCCG GCACCTGTCC TACGAGTTGC ATGATAAAGA AGACAGTCAT AAGTGCGGCG ACGATAGTCA TGCCCCGCGC CCACCGGAAG GAGCTGACTG GGTTGAAGGC TCTCAAGGGC ATCGGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAAT