SSGCID: Target Page

MyulA.19489.a

Dephospho-CoA kinase CoaE

CENTER ID:	MyulA.19489.a
ORGANISM:	Mycobacterium ulcerans Agy99
ASSOCIATED DISEASE:	Buruli, Bairnsdale ulcer
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	True
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyulA.19489.a.B1.GE41035	full length		1	407

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|362242.7.peg.1936
UniProt:	A0PP45

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MLRIGLTGGI GAGKSALSAT FAQCGGIIVD GDVLAREVVE PGTEGLAALV DAFGHDILLP DGALDRPGLA AKAFRDEASR SKLNGIVHPL VARRRAEIIA AVSDDAVVVE DIPLLVESGM APLFPLVVVV HADMEVRLRR LVHQRGMSED DACARIAAQA SDEQRRAVAD VWLDNSGSLD ALVERARDVW NNRILPFAHN LSVGQIARFP ARVVPADPSW PEQAQRIVAR LKTTCGHKAS RVDHIGSTAV AGFDAKDVID IQVTVESLAV ADELAEGLRS ASYPRLEHIT QDNPKEDPRS LIDHYDHGRD PALWHKRFHA SADPGRPTNV HIRVDGWPNQ QFALLFVDWL RANPGARADY LAIKRDAEKQ AALAGGGVEH YVTTKEPWFL DAYRRAWEWA DSTGWRP

NT Sequence

atgctgcgca tcgggcttac cggtggcatc ggtgccggaa agtcggcgct gtctgcgaca ttcgcacaat gtggcggcat catcgtcgac ggcgatgtgc tggctcgcga agtagttgag ccgggtaccg aaggactggc cgcgctggtc gatgccttcg gccatgacat cctgctgccc gatggggcac tggatcggcc ggggctggct gccaaggcgt ttcgggatga ggcgtcacgc tcgaagctca acggcatcgt gcacccgctg gtggcccgcc gtcgcgccga gatcatcgcg gcagtatccg atgacgcggt tgtcgtagag gacattccgc tgctggtgga atcggggatg gctccgctgt tcccgctcgt ggttgtcgtg catgccgaca tggaggtacg gcttcgacga ctggtccacc agcgcggcat gtccgaagac gatgcctgtg ccaggattgc cgcacaggct agcgatgagc agcgccgtgc cgtcgccgat gtctggctag acaattccgg cagcctcgat gcgttggtcg agcgtgcgcg ggacgtgtgg aacaacagga tcctgccttt cgcccacaac ctgagcgtgg gtcagattgc gcggtttcct gcccgtgtgg tgccggcaga cccaagttgg ccagagcagg cgcagcgcat cgttgcgcgc ctcaagacca catgcggtca caaggcgtcg cgggtcgacc acatcggctc gaccgctgtc gcgggattcg atgccaagga cgtcatcgac atccaggtca ccgtcgaatc acttgcggta gcagacgaac tggccgaggg gctgcgctcg gccagttatc cgcgccttga gcacatcacc caggacaacc ccaaagaaga cccgcgcagc ctcatcgacc actacgacca cggtcgtgac cctgcgttgt ggcataagcg ttttcatgcc tccgcggacc caggccgacc gacgaatgtg cacattcggg tggatggctg gcccaatcag cagttcgccc tgttgttcgt ggactggctg cgggccaatc ccggggcccg cgccgattat ctggccatca agcgcgacgc agaaaagcag gcggcgctcg ctggcggcgg cgtggagcat tacgtcacca caaaggagcc ctggtttctc gacgcctacc ggcgggcatg ggagtgggcc gactcgaccg gatggcggcc gtag

Details for MyulA.19489.a.B1.GE41035

HARVESTED ON:	1/4/2017
SEQUENCED ON:	1/5/2017
EXPECTED MW:	45kDa
OBSERVED MW:	45kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Good (10-50)
TOTAL EXPRESSION LEVEL:	Moderate Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY:	100
PERCENT COVERAGE:	98

Validated AA Sequence

MAHHHHHHML RIGLTGGIGA GKSALSATFA QCGGIIVDGD VLAREVVEPG TEGLAALVDA FGHDILLPDG ALDRPGLAAK AFRDEASRSK LNGIVHPLVA RRRAEIIAAV SDDAVVVEDI PLLVESGMAP LFPLVVVVHA DMEVRLRRLV HQRGMSEDDA CARIAAQASD EQRRAVADVW LDNSGSLDAL VERARDVWNN RILPFAHNLS VGQIARFPAR VVPADPSWPE QAQRIVARLK TTCGHKASRV DHIGSTAVAG FDAKDVIDIQ VTVESLAVAD ELAEGLRSAS YPRLEHITQD NPKEDPRSLI DHYDHGRDPA LWHKRFHASA DPGRPTNVHI RVDGWPNQQF ALLFVDWLRA NPGARADYLA IKRDAEKQAA LAGGGVEHYV TTKEPWFLDA YRRAWEW

Validated NT Sequence

gcccactccc atgcccgccg gtaggcgtcg agaaaccagg gctcctttgt ggtgacgtaa tgctccacgc cgccgccagc gagcgccgcc tgcttttctg cgtcgcgctt gatggccaga taatcggcgc gggccccggg attggcccgc agccagtcca cgaacaacag ggcgaactgc tgattgggcc agccatccac ccgaatgtgc acattcgtcg gtcggcctgg gtccgcggag gcatgaaaac gcttatgcca caacgcaggg tcacgaccgt ggtcgtagtg gtcgatgagg ctgcgcgggt cttctttggg gttgtcctgg gtgatgtgct caaggcgcgg ataactggcc gagcgcagcc cctcggccag ttcgtctgct accgcaagtg attcgacggt gacctggatg tcgatgacgt ccttggcatc gaatcccgcg acagcggtcg agccgatgtg gtcgacccgc gacgccttgt gaccgcatgt ggtcttgagg cgcgcaacga tgcgctgcgc ctgctctggc caacttgggt ctgccggcac cacacgggca ggaaaccgcg caatctgacc cacgctcagg ttgtgggcga aaggcaggat cctgttgttc cacacgtccc gcgcacgctc gaccaacgca tcgaggctgc cggaattgtc tagccagaca tcggcgacgg cacggcgctg ctcatcgcta gcctgtgcgg caatcctggc acaggcatcg tcttcggaca tgccgcgctg gtggaccagt cgtcgaagcc gtacctccat gtcggcatgc acgacaacca cgagcgggaa cagcggagcc atccccgatt ccaccagcag cggaatgtcc tctacgacaa ccgcgtcatc ggatactgcc gcgatgatct cggcgcgacg gcgggccacc agcgggtgca cgatgccgtt gagcttcgag cgtgacgcct catcccgaaa cgccttggca gccagccccg gccgatccag tgccccatcg ggcagcagga tgtcatggcc gaaggcatcg accagcgcgg ccagtccttc ggtacccggc tcaactactt cgcgagccag cacatcgccg tcgacgatga tgccgccaca ttgtgcgaat gtcgcagaca gcgccgactt tccggcaccg atgccaccgg taagcccgat gcgcagcata tggtggtggt ggtggtgagc cat

Expected Protein Sequence

Full NT Sequence (Expression Vector + Insert)

taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catatgctgc gcatcgggct taccggtggc atcggtgccg gaaagtcggc gctgtctgcg acattcgcac aatgtggcgg catcatcgtc gacggcgatg tgctggctcg cgaagtagtt gagccgggta ccgaaggact ggccgcgctg gtcgatgcct tcggccatga catcctgctg cccgatgggg cactggatcg gccggggctg gctgccaagg cgtttcggga tgaggcgtca cgctcgaagc tcaacggcat cgtgcacccg ctggtggccc gccgtcgcgc cgagatcatc gcggcagtat ccgatgacgc ggttgtcgta gaggacattc cgctgctggt ggaatcgggg atggctccgc tgttcccgct cgtggttgtc gtgcatgccg acatggaggt acggcttcga cgactggtcc accagcgcgg catgtccgaa gacgatgcct gtgccaggat tgccgcacag gctagcgatg agcagcgccg tgccgtcgcc gatgtctggc tagacaattc cggcagcctc gatgcgttgg tcgagcgtgc gcgggacgtg tggaacaaca ggatcctgcc tttcgcccac aacctgagcg tgggtcagat tgcgcggttt cctgcccgtg tggtgccggc agacccaagt tggccagagc aggcgcagcg catcgttgcg cgcctcaaga ccacatgcgg tcacaaggcg tcgcgggtcg accacatcgg ctcgaccgct gtcgcgggat tcgatgccaa ggacgtcatc gacatccagg tcaccgtcga atcacttgcg gtagcagacg aactggccga ggggctgcgc tcggccagtt atccgcgcct tgagcacatc acccaggaca accccaaaga agacccgcgc agcctcatcg accactacga ccacggtcgt gaccctgcgt tgtggcataa gcgttttcat gcctccgcgg acccaggccg accgacgaat gtgcacattc gggtggatgg ctggcccaat cagcagttcg ccctgttgtt cgtggactgg ctgcgggcca atcccggggc ccgcgccgat tatctggcca tcaagcgcga cgcagaaaag caggcggcgc tcgctggcgg cggcgtggag cattacgtca ccacaaagga gccctggttt ctcgacgcct accggcgggc atgggagtgg gccgactcga ccggatggcg gccgtgagta agataggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gcaaggagat ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatcga gatctcgatc ccgcgaaat