MyulA.18684.a

Pup--protein ligase (PafA)

CENTER ID: MyulA.18684.a
ORGANISM: Mycobacterium ulcerans Agy99
ASSOCIATED DISEASE: Buruli, Bairnsdale ulcer
CURRENT STATUS: cloned
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

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Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyulA.18684.a.A1.GE45372 full length 1 452
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.
NOTE: Effective as of September 1, 2026, SSGCID will no longer ship physical clone glycerols. SSGCID will provide amino acid and nucleotide sequences for any clones ordered, which the ordering party can then use to order the clone from a third-party clone manufacturer.

External Resources

RESOURCE REFERENCE ID
UniProt: A0PQT1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MQRRIMGIET EFGVTCTFHG HRRLSPDEVA RYLFRRVVSW GRSSNVFLRN GARLYLDVGS HPEYATAECD SLVQLVTHDR AGEWVLEDLL VDAEQRLADE GIGGDIYLFK NNTDSAGNSY GCHENYLIVR AGEFSRISDV LLPFLVTRQL ICGAGKVLQT PKAATFCLSQ RAEHIWEGVS SATTRSRPII NTRDEPHADA EKYRRLHVIV GDSNMCETTT MLKVGTASLV LEMIEAGVPF RDFSLDNPIR AIREVSHDVT GRRPVRLAGG RQASALDIQR EYYSRAVEHL QTREPNAQVE QVVDLWGRQL DAVESQDFAK VDTEIDWVIK RKLFQRYQDR YNMELSDPKI AQLDLAYHDI KRGRGVFDLL QRKGLATRVT TDEEIADAVD NPPQTTRARL RGEFISAAQA AGRDFTVDWV HLKLNDQAQR TVLCKDPFRA VDERVKRLIA SM
NT Sequence
gtgcagcgac gaatcatggg catcgagaca gagttcggtg tcacctgcac cttccacggt catcgccggc tgagccctga cgaggttgcc cggtacctat tccgcagggt ggtgtcctgg gggcgcagct ccaacgtatt cctgcgcaat ggcgcgcgtc tctacctcga cgtgggcagc catccggaat acgccaccgc cgaatgcgac agcctggtgc agctggttac ccacgaccgg gctggcgaat gggtgctcga agacctgctg gtcgacgccg agcagcggtt ggccgacgag ggcatcggcg gcgacatcta cctgttcaag aacaacaccg actcggccgg caactcctac ggttgccacg agaactacct gatcgtgcgg gccggcgagt tttcccggat ctccgacgtg ttgctgccgt tcctggtcac tcgtcagctg atctgtgggg cgggaaaagt gttgcagacc cccaaggccg caacattttg tctgtcccaa cgcgccgaac atatctggga gggcgtctcg agcgcgacca cccgctcacg tccgatcatc aacacccgcg acgagccgca cgccgacgcg gagaagtacc ggcgtctgca cgtgatcgtc ggcgactcca acatgtgcga gacgaccacc atgctcaagg tgggcaccgc gtcgttggtg ctggagatga tcgaagcggg agtgccgttc cgcgacttct cgctggacaa ccccatccgt gcgatccgtg aagtcagcca cgacgtcacc ggtcggcgtc cggttcggct ggccggcggg cgtcaggcca gtgcgctcga cattcagcgg gagtactact cccgcgccgt cgagcatctg cagacccggg agcccaacgc acaggtcgag caggtcgttg atctgtgggg ccgccagctt gacgccgtcg aaagccagga tttcgcgaag gtcgacaccg agatcgactg ggtgatcaag cgcaagctgt tccagcgtta ccaggaccgc tacaacatgg agctgtcgga ccccaagatc gcccagctcg atctggccta ccacgacatc aagcgcgggc gcggcgtctt cgatctgcta cagcgcaagg gcctggcgac acgcgtcacc accgacgagg aaatcgccga cgccgtcgac aacccaccac aaaccacccg cgccaggctg cgcggtgagt tcatcagcgc cgcacaggcg gccggtcgtg atttcaccgt cgactgggtg cacctcaagc tcaatgacca ggcgcagcgc acggtgctct gcaaggaccc attccgggcg gtcgacgagc gggtcaagcg gctgatcgcg agcatgtag
Details for MyulA.18684.a.A1.GE45372
HARVESTED ON: data unavailable
SEQUENCED ON: data unavailable
EXPECTED MW: 54kDa
OBSERVED MW: 0kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: BL 21 (DE3) Rosetta
SEQUENCING RESULT:
PERCENT IDENTITY: data unavailable
PERCENT COVERAGE: data unavailable
Validated AA Sequence
sequence unavailable
Validated NT Sequence
sequence unavailable
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMQRRIMGIE TEFGVTCTFH GHRRLSPDEV ARYLFRRVVS WGRSSNVFLR NGARLYLDVG SHPEYATAEC DSLVQLVTHD RAGEWVLEDL LVDAEQRLAD EGIGGDIYLF KNNTDSAGNS YGCHENYLIV RAGEFSRISD VLLPFLVTRQ LICGAGKVLQ TPKAATFCLS QRAEHIWEGV SSATTRSRPI INTRDEPHAD AEKYRRLHVI VGDSNMCETT TMLKVGTASL VLEMIEAGVP FRDFSLDNPI RAIREVSHDV TGRRPVRLAG GRQASALDIQ REYYSRAVEH LQTREPNAQV EQVVDLWGRQ LDAVESQDFA KVDTEIDWVI KRKLFQRYQD RYNMELSDPK IAQLDLAYHD IKRGRGVFDL LQRKGLATRV TTDEEIADAV DNPPQTTRAR LRGEFISAAQ AAGRDFTVDW VHLKLNDQAQ RTVLCKDPFR AVDERVKRLI ASM
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcggt gcagcgacga atcatgggca tcgagacaga gttcggtgtc acctgcacct tccacggtca tcgccggctg agccctgacg aggttgcccg gtacctattc cgcagggtgg tgtcctgggg gcgcagctcc aacgtattcc tgcgcaatgg cgcgcgtctc tacctcgacg tgggcagcca tccggaatac gccaccgccg aatgcgacag cctggtgcag ctggttaccc acgaccgggc tggcgaatgg gtgctcgaag acctgctggt cgacgccgag cagcggttgg ccgacgaggg catcggcggc gacatctacc tgttcaagaa caacaccgac tcggccggca actcctacgg ttgccacgag aactacctga tcgtgcgggc cggcgagttt tcccggatct ccgacgtgtt gctgccgttc ctggtcactc gtcagctgat ctgtggggcg ggaaaagtgt tgcagacccc caaggccgca acattttgtc tgtcccaacg cgccgaacat atctgggagg gcgtctcgag cgcgaccacc cgctcacgtc cgatcatcaa cacccgcgac gagccgcacg ccgacgcgga gaagtaccgg cgtctgcacg tgatcgtcgg cgactccaac atgtgcgaga cgaccaccat gctcaaggtg ggcaccgcgt cgttggtgct ggagatgatc gaagcgggag tgccgttccg cgacttctcg ctggacaacc ccatccgtgc gatccgtgaa gtcagccacg acgtcaccgg tcggcgtccg gttcggctgg ccggcgggcg tcaggccagt gcgctcgaca ttcagcggga gtactactcc cgcgccgtcg agcatctgca gacccgggag cccaacgcac aggtcgagca ggtcgttgat ctgtggggcc gccagcttga cgccgtcgaa agccaggatt tcgcgaaggt cgacaccgag atcgactggg tgatcaagcg caagctgttc cagcgttacc aggaccgcta caacatggag ctgtcggacc ccaagatcgc ccagctcgat ctggcctacc acgacatcaa gcgcgggcgc ggcgtcttcg atctgctaca gcgcaagggc ctggcgacac gcgtcaccac cgacgaggaa atcgccgacg ccgtcgacaa cccaccacaa accacccgcg ccaggctgcg cggtgagttc atcagcgccg cacaggcggc cggtcgtgat ttcaccgtcg actgggtgca cctcaagctc aatgaccagg cgcagcgcac ggtgctctgc aaggacccat tccgggcggt cgacgagcgg gtcaagcggc tgatcgcgag catgtaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa