MyulA.01626.a

Putative uncharacterized protein

CENTER ID: MyulA.01626.a
ORGANISM: Mycobacterium ulcerans Agy99
ASSOCIATED DISEASE: Buruli, Bairnsdale ulcer
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyulA.01626.a.A1.GE29942 Full length( MyulA.01626.a ) 1 399
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|362242.7.peg.349
RefSeq: YP_904447.1
UniProt: A0PKV7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSDVNGQQTG WNDRDGGDVQ VFELGTAPDF PTSVEIAVAN GPIGDIGISP DGSRLMVTNY GDNSVSVIDT DTCRVVETIT GLDEAFAVTA GGPAANRAYV STVSTAYDAI AVIDVATNAV VSTHPLALSV SDLAASSDGK YVYASRNGAR GADIAVLDTA TGRVDVVDIA TKPGLTTECV RVSADGNRVD VGANGPSGGQ LIVVATHAES ELNPVRGGRA RSRGRNGQGV AGSVVQQFPR DLRIIDTIEI GSSVRDVAIS PDGTTAYVAS CGADFGAVID VVDTRTHKIT GTRKIEEIGG LITRLSIGGN GERAYLVSED RVTVLSARSQ DVIGTVRVNQ PSAAIESPGG DRLYVADYSG TVTMTLVDLG VSAIDNALCQ GLSADWVMPE LLQFEAALA
NT Sequence
GTGAGCGACG TGAACGGCCA GCAGACCGGC TGGAATGATC GCGATGGCGG GGATGTCCAG GTCTTCGAGT TGGGTACCGC ACCCGATTTC CCGACCTCCG TCGAGATTGC GGTGGCCAAC GGGCCCATCG GTGACATCGG CATCAGTCCC GACGGCAGTC GCCTGATGGT GACCAACTAC GGCGACAACA GCGTGTCGGT GATCGACACC GACACCTGCC GGGTGGTGGA GACCATCACC GGTCTCGATG AGGCTTTCGC GGTCACCGCG GGCGGCCCAG CCGCCAACCG CGCCTACGTG AGCACCGTTT CGACGGCGTA TGACGCCATT GCCGTCATCG ATGTCGCTAC CAACGCCGTC GTCTCCACCC ACCCGCTGGC GCTCAGCGTC AGTGACCTGG CGGCCAGCTC GGATGGAAAG TACGTCTACG CCAGCCGTAA CGGCGCTCGC GGTGCCGACA TCGCTGTTCT TGACACCGCA ACGGGCCGCG TCGATGTGGT CGACATCGCT ACCAAGCCTG GCCTCACCAC CGAATGTGTC CGCGTCAGCG CCGACGGCAA CCGCGTTGAC GTCGGCGCCA ACGGACCGTC CGGCGGTCAG CTCATCGTCG TGGCAACGCA TGCGGAGTCC GAACTCAATC CGGTCCGGGG CGGACGCGCA CGCTCGCGGG GCAGGAATGG CCAGGGCGTG GCCGGATCTG TCGTGCAGCA GTTCCCGCGC GATCTGCGCA TCATCGACAC GATTGAGATC GGCTCTTCGG TTCGTGATGT CGCGATCAGC CCCGACGGCA CCACCGCCTA CGTCGCCAGT TGCGGCGCCG ACTTCGGCGC GGTGATCGAC GTTGTCGACA CCCGCACCCA CAAGATCACC GGCACCCGCA AGATCGAGGA GATCGGCGGT CTGATCACCC GGCTTTCCAT CGGCGGCAAC GGCGAGCGTG CCTACCTGGT GAGCGAGGAC CGTGTCACCG TGCTCAGCGC GCGCTCGCAG GATGTCATCG GCACCGTCCG GGTCAACCAG CCGTCCGCCG CGATCGAAAG CCCGGGCGGC GACCGCCTGT ACGTCGCTGA CTATTCCGGA ACGGTCACCA TGACGTTGGT TGATCTGGGC GTGTCAGCCA TCGACAACGC CCTGTGCCAG GGTCTGTCGG CCGATTGGGT CATGCCTGAG CTGCTGCAGT TCGAGGCGGC GCTGGCC
Details for MyulA.01626.a.A1.GE29942
HARVESTED ON: 7/13/2010
SEQUENCED ON: 8/20/2010
EXPECTED MW: 43kDa
OBSERVED MW: 49kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 66
Validated AA Sequence
MVTNYGDNSV SVIDTDTCRV VETITGLDEA FAVTAGGPAA NRAYVSTVST AYDAIAVIDV ATNAVVSTHP LALSVSDLAA SSDGKYVYAS RNGARGADIA VLDTATGRVD VVDIATKPGL TTECVRVSAD GNRVDVGANG PSGGQLIVVA THAESELNPV RGGRARSRGR NGQGVAGSVV QQFPRDLRII DTIEIGSSVR DVAISPDGTT AYVASCGADF GAVIDVVDTR THKITGTRKI EEIGGLITRL SIGGNGERAY LVSEDRVTVL SARSQDVIGT
Validated NT Sequence
gacggcagtc gcctgatggt gaccaactac ggcgacaaca gcgtgtcggt gatcgacacc gacacctgcc gggtggtgga gaccatcacc ggtctcgatg aggctttcgc ggtcaccgcg ggcggcccag ccgccaaccg cgcctacgtg agcaccgttt cgacggcgta tgacgccatt gccgtcatcg atgtcgctac caacgccgtc gtctccaccc acccgctggc gctcagcgtc agtgacctgg cggccagctc ggatggaaag tacgtctacg ccagccgtaa cggcgctcgc ggtgccgaca tcgctgttct tgacaccgca acgggccgcg tcgatgtggt cgacatcgct accaagcctg gcctcaccac cgaatgtgtc cgcgtcagcg ccgacggcaa ccgcgttgac gtcggcgcca acggaccgtc cggcggtcag ctcatcgtcg tggcaacgca tgcggagtcc gaactcaatc cggtccgggg cggacgcgca cgctcgcggg gcaggaatgg ccagggcgtg gccggatctg tcgtgcagca gttcccgcgc gatctgcgca tcatcgacac gattgagatc ggctcttcgg ttcgtgatgt cgcgatcagc cccgacggca ccaccgccta cgtcgccagt tgcggcgccg acttcggcgc ggtgatcgac gttgtcgaca cccgcaccca caagatcacc ggcacccgca agatcgagga gatcggcggt ctgatcaccc ggctttccat cggcggcaac ggcgagcgtg cctacctggt gagcgaggac cgtgtcaccg tgctcagcgc gcgctcgcag gatgtcatcg gcaccgtc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVSDVNGQQ TGWNDRDGGD VQVFELGTAP DFPTSVEIAV ANGPIGDIGI SPDGSRLMVT NYGDNSVSVI DTDTCRVVET ITGLDEAFAV TAGGPAANRA YVSTVSTAYD AIAVIDVATN AVVSTHPLAL SVSDLAASSD GKYVYASRNG ARGADIAVLD TATGRVDVVD IATKPGLTTE CVRVSADGNR VDVGANGPSG GQLIVVATHA ESELNPVRGG RARSRGRNGQ GVAGSVVQQF PRDLRIIDTI EIGSSVRDVA ISPDGTTAYV ASCGADFGAV IDVVDTRTHK ITGTRKIEEI GGLITRLSIG GNGERAYLVS EDRVTVLSAR SQDVIGTVRV NQPSAAIESP GGDRLYVADY SGTVTMTLVD LGVSAIDNAL CQGLSADWVM PELLQFEAAL
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgagcgac gtgaacggcc agcagaccgg ctggaatgat cgcgatggcg gggatgtcca ggtcttcgag ttgggtaccg cacccgattt cccgacctcc gtcgagattg cggtggccaa cgggcccatc ggtgacatcg gcatcagtcc cgacggcagt cgcctgatgg tgaccaacta cggcgacaac agcgtgtcgg tgatcgacac cgacacctgc cgggtggtgg agaccatcac cggtctcgat gaggctttcg cggtcaccgc gggcggccca gccgccaacc gcgcctacgt gagcaccgtt tcgacggcgt atgacgccat tgccgtcatc gatgtcgcta ccaacgccgt cgtctccacc cacccgctgg cgctcagcgt cagtgacctg gcggccagct cggatggaaa gtacgtctac gccagccgta acggcgctcg cggtgccgac atcgctgttc ttgacaccgc aacgggccgc gtcgatgtgg tcgacatcgc taccaagcct ggcctcacca ccgaatgtgt ccgcgtcagc gccgacggca accgcgttga cgtcggcgcc aacggaccgt ccggcggtca gctcatcgtc gtggcaacgc atgcggagtc cgaactcaat ccggtccggg gcggacgcgc acgctcgcgg ggcaggaatg gccagggcgt ggccggatct gtcgtgcagc agttcccgcg cgatctgcgc atcatcgaca cgattgagat cggctcttcg gttcgtgatg tcgcgatcag ccccgacggc accaccgcct acgtcgccag ttgcggcgcc gacttcggcg cggtgatcga cgttgtcgac acccgcaccc acaagatcac cggcacccgc aagatcgagg agatcggcgg tctgatcacc cggctttcca tcggcggcaa cggcgagcgt gcctacctgg tgagcgagga ccgtgtcacc gtgctcagcg cgcgctcgca ggatgtcatc ggcaccgtcc gggtcaacca gccgtccgcc gcgatcgaaa gcccgggcgg cgaccgcctg tacgtcgctg actattccgg aacggtcacc atgacgttgg ttgatctggg cgtgtcagcc atcgacaacg ccctgtgcca gggtctgtcg gccgattggg tcatgcctga gctgctgcag ttcgaggcgg cgctgaaaca gcacgaacaa gttctgcagc caagcttctc gaggatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggata tccacaggac gggtgtggtc gccatgatcg cgtagtcgat agtggctcca agtagcgaag cgagcaggac tgggcggcgg ccaaagcggt cggacagtgc tccgagaacg ggtgcgcata gaaattgcat caacgcatat agcgctagca gcacgccata gtgactggcg atgctgtcgg aatggacgat atcccgcaag aggcccggca gtaccggcat aaccaagcct atgcctacag catccagggt gacggtgccg aggatgacga tgagcgcatt gttagatttc atacacggtg cctgactgcg ttagcaattt aactgtgata aactaccgca ttaaagctta tcgatgataa gctgtcaaac atgagaa