MyulA.01613.i

Acyl-CoA dehydrogenase

CENTER ID: MyulA.01613.i
ORGANISM: Mycobacterium ulcerans Agy99
ASSOCIATED DISEASE: Buruli, Bairnsdale ulcer
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyulA.01613.i.A1.GE29940 Full length( MyulA.01613.i ) 1 393
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|362242.7.peg.4518
RefSeq: YP_907426.1
UniProt: A0PUD6

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDFSRVQISD ADREFRETAR SFLATHVTEE VRRHDRETGD NFHEGLHLAL GAAGYLAAEW KPEADGGFSR VRRRIWELEK RRAHVPWVTW GTTAMVARSV AKFGSPELVE EVLPGVFSGH VRMCLGYTEP EGGSDVATCK TRALRDGDSW LINGSKMFTT GAHNCQYVFL ITNTDPQARK HKSLTMFLVP LDSAGIEIQG IRTVDGDRTN IVYYSDVRID DKYRLGAVNG GWTVLREPLN VEHGAVAAAP DGLQDTSIMM HQAGSMAEAV DRAASCVMRP DPDGRRPIDD TSVAYRLGRS VARMEAALSA PSIFGRVAIA QTMRDISPDL MDILGSAAAL PFGTDGAVDD GGAEYVYRFA PLVGIYGGTH EVFRNMIAQH VLGLGKPSYP HRT
NT Sequence
ATGGATTTCT CCCGAGTGCA GATTTCAGAC GCGGACCGCG AGTTTCGGGA AACGGCGCGT AGCTTCCTGG CCACCCATGT CACCGAGGAG GTTCGCCGGC ACGATCGTGA GACCGGCGAC AACTTCCATG AAGGTCTGCA TCTGGCGTTG GGCGCCGCAG GCTACTTGGC AGCGGAGTGG AAACCAGAGG CCGACGGCGG CTTCAGCCGG GTGCGGCGGC GCATCTGGGA GCTGGAAAAG CGGCGTGCCC ACGTGCCGTG GGTGACTTGG GGGACCACCG CCATGGTGGC GCGCTCGGTC GCGAAGTTCG GTTCGCCCGA GCTTGTCGAA GAGGTGCTCC CCGGTGTCTT CAGCGGCCAC GTCCGCATGT GTCTGGGGTA CACCGAGCCC GAGGGCGGCT CGGACGTCGC GACCTGCAAG ACCCGGGCGC TGCGCGACGG AGACAGCTGG CTGATCAACG GATCGAAAAT GTTCACCACT GGAGCGCACA ACTGCCAGTA CGTATTCCTG ATCACCAATA CTGACCCGCA GGCGCGAAAA CATAAGAGTC TCACGATGTT TCTGGTTCCG CTGGACTCTG CCGGCATCGA GATTCAGGGT ATCCGGACCG TCGACGGTGA CCGAACCAAT ATCGTCTACT ACAGCGACGT CCGGATAGAC GACAAATATC GGCTCGGTGC GGTGAATGGC GGATGGACGG TTCTGCGTGA GCCGCTCAAC GTCGAGCACG GCGCGGTTGC GGCGGCTCCC GATGGGCTGC AGGACACCTC GATCATGATG CACCAGGCCG GGTCCATGGC CGAAGCGGTC GACCGGGCCG CAAGCTGCGT GATGCGGCCA GACCCCGATG GACGCCGCCC CATCGACGAC ACTTCGGTGG CGTATCGGTT GGGGCGCAGT GTCGCCCGGA TGGAGGCCGC ATTGTCCGCT CCGAGCATTT TTGGCAGGGT CGCGATTGCC CAGACGATGC GCGACATTTC ACCGGATCTG ATGGACATTC TCGGCAGCGC CGCCGCACTA CCGTTCGGGA CCGATGGTGC GGTCGATGAC GGCGGCGCCG AATACGTCTA CCGTTTCGCC CCACTGGTCG GCATTTACGG CGGCACGCAC GAGGTGTTCC GCAACATGAT CGCCCAGCAC GTCCTGGGGC TCGGCAAACC GAGTTACCCG CACCGAACG
Details for MyulA.01613.i.A1.GE29940
HARVESTED ON: 7/13/2010
SEQUENCED ON: 8/18/2010
EXPECTED MW: 45kDa
OBSERVED MW: 45kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 95
PERCENT COVERAGE: 94
Validated AA Sequence
MDFSRVXISX XDRXFRETAR XFLATHVTEE VRRHDRXTGX NFHEGLHLAL GAAGYLAAXX KPXADGGFXR VRRRIWELEK RRAHXPWVTW GTTAXXARSV XKFGSPELVK XVLPGVFSGH VRMCLGYTEP EGGSDVATCK TRALRDGDSW LINGSKMFTT GAHNCQYVFL ITNTDPQARK HKSLTMFLVP LDSAGIEIQG IRTVDGDRTN IVYYSDVRID DKYRLGAVNG GWTVLREPLN VEHGAVAAAP DGLQDTSIMM HQAGSMAEAV DRAASCVMRP DPDGRRPIDD TSVAYRLGRS VARMEAALSA PSIFGRVAIA QTMRDISPDL MDILGSAAAL PFGTDGAVDD GGAEYVYRFA PLVGIYGGTH EVFRNMIAQH VLGLGKPSY
Validated NT Sequence
ccctggaagc tcanacccag ggtcctggtt cgatggattt ctcccgagtg canatttcan acncggaccg cnagtttcgg gaaacggcgc gtancttcct ggccacccat gtcaccgagg aggttcgccg gcacgatcgt ganaccggnn acaacttcca tgaaggtctg catctggcgt tgggcgccgc aggctacttg gcagcggant gnaaaccana ggccgacggc ggcttcnncc gggtgcggcg gcgcatctgg gagctggaaa agcggcgtgc ccacntgccn tgggtgactt gggggaccac cgccntggng gcgcgctcgg tcgngaagtt cggttcgccc gagcttgtca aanaggtgct ccccggtgtc ttcagcggcc acgtccgcat gtgtctgggg tacaccgagc ccgagggcgg ctcggacgtc gcgacctgca agacccgggc gctgcgcgac ggagacagct ggctgatcaa cggatcgaaa atgttcacca ctggagcgca caactgccag tacgtattcc tgatcaccaa tactgacccg caggcgcgaa aacataagag tctcacgatg tttctggttc cgctggactc tgccggcatc gagattcagg gtatccggac cgtcgacggt gaccgaacca atatcgtcta ctacagcgac gtccggatag acgacaaata tcggctcggt gcggtgaatg gcggatggac ggttctgcgt gagccgctca acgtcgagca cggcgcggtt gcggcggctc ccgatgggct gcaggacacc tcgatcatga tgcaccaggc cgggtccatg gccgaagcgg tcgaccgggc cgcaagctgc gtgatgcggc cagaccccga tggacgccgc cccatcgacg acacttcggt ggcgtatcgg ttggggcgca gtgtcgcccg gatggaggcc gcattgtccg ctccgagcat ttttggcagg gtcgcgattg cccagacgat gcgcgacatt tcaccggatc tgatggacat tctcggcagc gccgccgcac taccgttcgg gaccgatggt gcggtcgatg acggcggcgc cgaatacgtc taccgtttcg ccccactggt cggcatttac ggcggcacgc acgaggtgtt ccgcaacatg atcgcccagc acgtcctggg gctcggcaaa ccgagttacc cg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMDFSRVQIS DADREFRETA RSFLATHVTE EVRRHDRETG DNFHEGLHLA LGAAGYLAAE WKPEADGGFS RVRRRIWELE KRRAHVPWVT WGTTAMVARS VAKFGSPELV EEVLPGVFSG HVRMCLGYTE PEGGSDVATC KTRALRDGDS WLINGSKMFT TGAHNCQYVF LITNTDPQAR KHKSLTMFLV PLDSAGIEIQ GIRTVDGDRT NIVYYSDVRI DDKYRLGAVN GGWTVLREPL NVEHGAVAAA PDGLQDTSIM MHQAGSMAEA VDRAASCVMR PDPDGRRPID DTSVAYRLGR SVARMEAALS APSIFGRVAI AQTMRDISPD LMDILGSAAA LPFGTDGAVD DGGAEYVYRF APLVGIYGGT HEVFRNMIAQ HVLGLGKPSY PHR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggatttctcc cgagtgcaga tttcagacgc ggaccgcgag tttcgggaaa cggcgcgtag cttcctggcc acccatgtca ccgaggaggt tcgccggcac gatcgtgaga ccggcgacaa cttccatgaa ggtctgcatc tggcgttggg cgccgcaggc tacttggcag cggagtggaa accagaggcc gacggcggct tcagccgggt gcggcggcgc atctgggagc tggaaaagcg gcgtgcccac gtgccgtggg tgacttgggg gaccaccgcc atggtggcgc gctcggtcgc gaagttcggt tcgcccgagc ttgtcgaaga ggtgctcccc ggtgtcttca gcggccacgt ccgcatgtgt ctggggtaca ccgagcccga gggcggctcg gacgtcgcga cctgcaagac ccgggcgctg cgcgacggag acagctggct gatcaacgga tcgaaaatgt tcaccactgg agcgcacaac tgccagtacg tattcctgat caccaatact gacccgcagg cgcgaaaaca taagagtctc acgatgtttc tggttccgct ggactctgcc ggcatcgaga ttcagggtat ccggaccgtc gacggtgacc gaaccaatat cgtctactac agcgacgtcc ggatagacga caaatatcgg ctcggtgcgg tgaatggcgg atggacggtt ctgcgtgagc cgctcaacgt cgagcacggc gcggttgcgg cggctcccga tgggctgcag gacacctcga tcatgatgca ccaggccggg tccatggccg aagcggtcga ccgggccgca agctgcgtga tgcggccaga ccccgatgga cgccgcccca tcgacgacac ttcggtggcg tatcggttgg ggcgcagtgt cgcccggatg gaggccgcat tgtccgctcc gagcattttt ggcagggtcg cgattgccca gacgatgcgc gacatttcac cggatctgat ggacattctc ggcagcgccg ccgcactacc gttcgggacc gatggtgcgg tcgatgacgg cggcgccgaa tacgtctacc gtttcgcccc actggtcggc atttacggcg gcacgcacga ggtgttccgc aacatgatcg cccagcacgt cctggggctc ggcaaaccga gttacccgca ccgaaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa