MyulA.01613.h

Acyl-CoA dehydrogenase FadE17_1

CENTER ID: MyulA.01613.h
ORGANISM: Mycobacterium ulcerans Agy99
ASSOCIATED DISEASE: Buruli, Bairnsdale ulcer
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyulA.01613.h.A1.GE29939 Full length( MyulA.01613.h ) 1 388
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|362242.7.peg.4287
RefSeq: YP_907281.1
UniProt: A0PTZ1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKLALTPDEA AFRDELRTFY TTRIPEDLRE LVRQGQSPSR DEIVTCHKIL NDHGLAVPNW PVEWGGKDWT PTQHQIWADE MQLASVPEPL NFNTKMVGPV IAEFGSQEIK QRFLPPTASL DIWWCQGFSE PEAGSDLASL RTTAVRDGDS YVVNGQKTWT TLGQYADWIF CLVRTDPQAP KRQAGISFLL IDMATPGITL RPIKTIDGGH EVNEVFFSDV RVPADQLVGQ ENQGWTYAKF LLGNERTGIA GVGRTKVRLA EVKKHAANNG LLDDPLFAAR LAEAENELLA LELTQARVVT DSADGKPNPA SSVLKLRGSQ LQQVATELLV EVAGPDALPV GGAQAAGIAS PQWAQSSAPR YLNYRKTSIY GGSNEVQRNI IASTILGL
NT Sequence
ATGAAGCTGG CTCTCACCCC CGACGAAGCC GCCTTCCGCG ACGAGCTTCG CACCTTCTAC ACCACCCGGA TACCCGAAGA CCTGCGCGAG CTGGTGCGCC AGGGCCAGTC GCCGAGCCGC GATGAGATCG TCACGTGCCA CAAGATCCTC AACGATCACG GGCTGGCGGT GCCGAACTGG CCGGTGGAAT GGGGCGGTAA GGACTGGACA CCGACTCAGC ACCAGATCTG GGCCGACGAG ATGCAGCTGG CCAGCGTCCC GGAGCCGTTG AACTTCAACA CCAAGATGGT CGGTCCGGTG ATCGCCGAAT TCGGATCCCA GGAGATCAAG CAGCGCTTCC TGCCGCCGAC GGCCAGCCTC GACATCTGGT GGTGTCAAGG CTTCTCCGAA CCTGAAGCCG GTTCGGATCT CGCATCACTG CGCACCACCG CCGTGCGCGA TGGCGACAGC TACGTCGTCA ATGGCCAGAA GACATGGACG ACGCTGGGCC AGTATGCCGA CTGGATTTTC TGCCTGGTGC GCACCGACCC CCAGGCGCCC AAGCGGCAAG CCGGCATTTC GTTTCTGCTC ATCGACATGG CCACCCCCGG CATCACGCTG CGGCCGATCA AGACCATCGA CGGTGGTCAC GAAGTCAACG AGGTGTTTTT CTCCGATGTT CGAGTACCGG CCGATCAACT CGTTGGCCAG GAGAACCAGG GCTGGACCTA CGCGAAGTTC CTGCTGGGCA ACGAGCGCAC CGGGATCGCC GGGGTGGGCC GCACCAAGGT GCGCCTCGCC GAGGTGAAAA AGCACGCTGC CAACAACGGC CTGCTCGACG ATCCGCTGTT CGCGGCGCGC CTTGCTGAAG CCGAAAACGA GCTGCTGGCA TTGGAACTCA CCCAGGCTCG GGTGGTTACC GACTCGGCAG ACGGTAAGCC CAACCCGGCG TCGTCGGTAC TCAAGTTGCG CGGCAGCCAA TTGCAGCAAG TCGCCACCGA ATTGCTCGTC GAGGTAGCCG GGCCCGACGC GCTACCGGTG GGCGGTGCAC AGGCGGCAGG GATCGCGTCA CCGCAGTGGG CCCAGAGCAG CGCCCCGCGC TACCTGAACT ACCGCAAGAC ATCGATTTAC GGCGGCAGCA ACGAAGTGCA GCGCAACATC ATCGCGTCCA CCATCTTGGG ATTG
Details for MyulA.01613.h.A1.GE29939
HARVESTED ON: 7/13/2010
SEQUENCED ON: 8/20/2010
EXPECTED MW: 44kDa
OBSERVED MW: 45kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 94
Validated AA Sequence
MKLALTPDEA AFRDELRTFY TTRIPEDLRE LVRQGQSPSR DEIVTCHKIL NDHGLAVPNW PVEWGGKDWT PTQHQIWADE MQLASVPEPL NFNTKMVGPV IAEFGSQEIK QRFLPPTASL DIWWCQGFSE PEAGSDLASL RTTAVRDGDS YVVNGQKTWT TLGQYADWIF CLVRTDPQAP KRQAGISFLL IDMATPGITL RPIKTIDGGH EVNEVFFSDV RVPADQLVGQ ENQGWTYAKF LLGNERTGIA GVGRTKVRLA EVKKHAANNG LLDDPLFAAR LAEAENELLA LELTQARVVT DSADGKPNPA SSVLKLRGSQ LQQVATELLV EVAGPDALPV GGAQAAGIAS PQWAQSSAPR YLNYRKTSIY GGSNEVQRNI IASTIL
Validated NT Sequence
tggaagctca gacccagggt cctggttcga tgaagctggc tctcaccccc gacgaagccg ccttccgcga cgagcttcgc accttctaca ccacccggat acccgaagac ctgcgcgagc tggtgcgcca gggccagtcg ccgagccgcg atgagatcgt cacgtgccac aagatcctca acgatcacgg gctggcggtg ccgaactggc cggtggaatg gggcggtaag gactggacac cgactcagca ccagatctgg gccgacgaga tgcagctggc cagcgtcccg gagccgttga acttcaacac caagatggtc ggtccggtga tcgccgaatt cggatcccag gagatcaagc agcgcttcct gccgccgacg gccagcctcg acatctggtg gtgtcaaggc ttctccgaac ctgaagccgg ttcggatctc gcatcactgc gcaccaccgc cgtgcgcgat ggcgacagct acgtcgtcaa tggccagaag acatggacga cgctgggcca gtatgccgac tggattttct gcctggtgcg caccgacccc caggcgccca agcggcaagc cggcatttcg tttctgctca tcgacatggc cacccccggc atcacgctgc ggccgatcaa gaccatcgac ggtggtcacg aagtcaacga ggtgtttttc tccgatgttc gagtaccggc cgatcaactc gttggccagg agaaccaggg ctggacctac gcgaagttcc tgctgggcaa cgagcgcacc gggatcgccg gggtgggccg caccaaggtg cgcctcgccg aggtgaaaaa gcacgctgcc aacaacggcc tgctcgacga tccgctgttc gcggcgcgcc ttgctgaagc cgaaaacgag ctgctggcat tggaactcac ccaggctcgg gtggttaccg actcggcaga cggtaagccc aacccggcgt cgtcggtact caagttgcgc ggcagccaat tgcagcaagt cgccaccgaa ttgctcgtcg aggtagccgg gcccgacgcg ctaccggtgg gcggtgcaca ggcggcaggg atcgcgtcac cgcagtgggc ccagagcagc gccccgcgct acctgaacta ccgcaagaca tcgatttacg gcggcagcaa cgaagtgcag cgcaacatca tcgcgtccac catcttggga
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMKLALTPDE AAFRDELRTF YTTRIPEDLR ELVRQGQSPS RDEIVTCHKI LNDHGLAVPN WPVEWGGKDW TPTQHQIWAD EMQLASVPEP LNFNTKMVGP VIAEFGSQEI KQRFLPPTAS LDIWWCQGFS EPEAGSDLAS LRTTAVRDGD SYVVNGQKTW TTLGQYADWI FCLVRTDPQA PKRQAGISFL LIDMATPGIT LRPIKTIDGG HEVNEVFFSD VRVPADQLVG QENQGWTYAK FLLGNERTGI AGVGRTKVRL AEVKKHAANN GLLDDPLFAA RLAEAENELL ALELTQARVV TDSADGKPNP ASSVLKLRGS QLQQVATELL VEVAGPDALP VGGAQAAGIA SPQWAQSSAP RYLNYRKTSI YGGSNEVQRN IIASTILG
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaagctggct ctcacccccg acgaagccgc cttccgcgac gagcttcgca ccttctacac cacccggata cccgaagacc tgcgcgagct ggtgcgccag ggccagtcgc cgagccgcga tgagatcgtc acgtgccaca agatcctcaa cgatcacggg ctggcggtgc cgaactggcc ggtggaatgg ggcggtaagg actggacacc gactcagcac cagatctggg ccgacgagat gcagctggcc agcgtcccgg agccgttgaa cttcaacacc aagatggtcg gtccggtgat cgccgaattc ggatcccagg agatcaagca gcgcttcctg ccgccgacgg ccagcctcga catctggtgg tgtcaaggct tctccgaacc tgaagccggt tcggatctcg catcactgcg caccaccgcc gtgcgcgatg gcgacagcta cgtcgtcaat ggccagaaga catggacgac gctgggccag tatgccgact ggattttctg cctggtgcgc accgaccccc aggcgcccaa gcggcaagcc ggcatttcgt ttctgctcat cgacatggcc acccccggca tcacgctgcg gccgatcaag accatcgacg gtggtcacga agtcaacgag gtgtttttct ccgatgttcg agtaccggcc gatcaactcg ttggccagga gaaccagggc tggacctacg cgaagttcct gctgggcaac gagcgcaccg ggatcgccgg ggtgggccgc accaaggtgc gcctcgccga ggtgaaaaag cacgctgcca acaacggcct gctcgacgat ccgctgttcg cggcgcgcct tgctgaagcc gaaaacgagc tgctggcatt ggaactcacc caggctcggg tggttaccga ctcggcagac ggtaagccca acccggcgtc gtcggtactc aagttgcgcg gcagccaatt gcagcaagtc gccaccgaat tgctcgtcga ggtagccggg cccgacgcgc taccggtggg cggtgcacag gcggcaggga tcgcgtcacc gcagtgggcc cagagcagcg ccccgcgcta cctgaactac cgcaagacat cgatttacgg cggcagcaac gaagtgcagc gcaacatcat cgcgtccacc atcttgggaa aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a