MyulA.01613.f

Acyl-CoA dehydrogenase

CENTER ID: MyulA.01613.f
ORGANISM: Mycobacterium ulcerans Agy99
ASSOCIATED DISEASE: Buruli, Bairnsdale ulcer
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyulA.01613.f.A1.GE29937 Full length( MyulA.01613.f ) 1 392
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|362242.7.peg.3221
RefSeq: YP_906534.1
UniProt: A0PRU4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MALPRLVPPA GALDSPRADV LRGEVRAFLA EQSAAGGFTP SVDAWLSAWD EDFTAALADR GWLGMTVPVE YGGHGRTFLE RFVVTEELLA VGAPVAAHWI ADRQIVPSLL KYGTELQKSH FLPRIVRGEC FFGIGMSEPD SGSDLASVRT RAVRGEGGWL LSGTKVWTSG AHRAHAFIVL ARTAPVDPDH RHAGLSQFIV DLRGPGVQTR PIISMNGAHH FNEVILDNAH VPDEMVFGAI GNGWTQVTSE LSFERSGPER FLSTFVLLAA TAESMAEQRF TRDQQLGRLV ARIAGLHQMS AAVAGALERN DPADLPAAVV KVLGTSTESD IAELADLADS TDLADSGAPH RPRDPEFAAL VTAALDQRPG FTLRGGTNEV LRGVIARGLG LR
NT Sequence
ATGGCGCTGC CCCGGTTGGT GCCACCCGCG GGGGCCCTCG ATTCCCCCCG GGCCGATGTC CTGCGCGGCG AGGTCCGCGC ATTTCTTGCC GAGCAGTCCG CCGCCGGCGG GTTCACCCCC TCGGTTGATG CCTGGCTGTC GGCGTGGGAT GAGGACTTCA CCGCCGCGCT CGCCGACCGC GGCTGGTTGG GCATGACCGT GCCCGTCGAG TACGGCGGAC ACGGGCGCAC GTTCCTGGAA CGATTCGTCG TCACCGAAGA ACTGCTGGCC GTCGGTGCAC CCGTGGCCGC CCATTGGATA GCCGACCGTC AAATCGTGCC GTCACTGCTC AAGTACGGGA CCGAACTGCA GAAGTCGCAC TTCCTGCCTC GGATTGTGCG CGGTGAGTGC TTTTTCGGCA TCGGCATGAG CGAGCCCGAT TCCGGTTCCG ATCTGGCCAG CGTGCGGACC CGGGCGGTAC GCGGCGAGGG CGGTTGGCTG TTGTCGGGCA CAAAGGTATG GACCTCGGGG GCACACCGCG CCCACGCGTT CATCGTGCTC GCGCGCACCG CGCCGGTGGA CCCCGACCAC AGGCATGCCG GGCTAAGTCA ATTCATCGTC GACCTACGCG GGCCGGGGGT CCAGACCCGG CCGATCATCT CCATGAATGG CGCCCACCAC TTCAACGAGG TGATCCTCGA CAACGCCCAT GTACCCGACG AGATGGTGTT CGGGGCAATC GGCAACGGCT GGACGCAGGT GACATCGGAA CTCAGCTTCG AGCGCAGTGG ACCAGAGCGC TTCCTGTCCA CCTTCGTATT GCTGGCTGCT ACCGCCGAAA GCATGGCCGA GCAACGCTTT ACCCGCGACC AGCAGCTCGG GCGACTGGTG GCGCGCATCG CCGGCCTGCA CCAGATGTCC GCCGCGGTGG CTGGCGCACT TGAGCGCAAC GATCCCGCCG ACCTACCTGC GGCGGTGGTC AAGGTGCTGG GCACCAGCAC CGAGAGCGAC ATAGCCGAAC TCGCCGACCT GGCCGACTCG ACCGACCTGG CCGACTCGGG CGCCCCGCAC CGCCCGCGCG ATCCCGAGTT CGCCGCCCTG GTCACCGCGG CGCTGGACCA GCGGCCGGGC TTCACCCTGC GTGGTGGAAC CAACGAAGTG CTGCGCGGTG TCATCGCGCG GGGATTGGGA CTGCGA
Details for MyulA.01613.f.A1.GE29937
HARVESTED ON: 7/13/2010
SEQUENCED ON: 8/20/2010
EXPECTED MW: 44kDa
OBSERVED MW: 45kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Low Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 98
Validated AA Sequence
MGTLEAQTQG PGSMALPRLV PPAGALDSPR ADVLRGEVRA FLAEQSAAGG FTPSVDAWLS AWDEDFTAAL ADRGWLGMTV PVEYGGHGRT FLERFVVTEE LLAVGAPVAA HWIADRQIVP SLLKYGTELQ KSHFLPRIVR GECFFGIGMS EPDSGSDLAS VRTRAVRGEG GWLLSGTKVW TSGAHRAHAF IVLARTAPVD PDHRHAGLSQ FIVDLRGPGV QTRPIISMNG AHHFNEVILD NAHVPDEMVF GAIGNGWTQV TSELSFERSG PERFLSTFVL LAATAESMAE QRFTRDQQLG RLVARIAGLH QMSAAVAGAL ERNDPADLPA AVVKVLGTST ESDIAELADL ADSTDLADSG APHRPRDPEF AALVTAALDQ RPGFTLRGGT NEVLRGVIAR GLGL
Validated NT Sequence
tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatggcgct gccccggttg gtgccacccg cgggggccct cgattccccc cgggccgatg tcctgcgcgg cgaggtccgc gcatttcttg ccgagcagtc cgccgccggc gggttcaccc cctcggttga tgcctggctg tcggcgtggg atgaggactt caccgccgcg ctcgccgacc gcggctggtt gggcatgacc gtgcccgtcg agtacggcgg acacgggcgc acgttcctgg aacgattcgt cgtcaccgaa gaactgctgg ccgtcggtgc acccgtggcc gcccattgga tagccgaccg tcaaatcgtg ccgtcactgc tcaagtacgg gaccgaactg cagaagtcgc acttcctgcc tcggattgtg cgcggtgagt gctttttcgg catcggcatg agcgagcccg attccggttc cgatctggcc agcgtgcgga cccgggcggt acgcggcgag ggcggttggc tgttgtcggg cacaaaggta tggacctcgg gggcacaccg cgcccacgcg ttcatcgtgc tcgcgcgcac cgcgccggtg gaccccgacc acaggcatgc cgggctaagt caattcatcg tcgacctacg cgggccgggg gtccagaccc ggccgatcat ctccatgaat ggcgcccacc acttcaacga ggtgatcctc gacaacgccc atgtacccga cgagatggtg ttcggggcaa tcggcaacgg ctggacgcag gtgacatcgg aactcagctt cgagcgcagt ggaccagagc gcttcctgtc caccttcgta ttgctggctg ctaccgccga aagcatggcc gagcaacgct ttacccgcga ccagcagctc gggcgactgg tggcgcgcat cgccggcctg caccagatgt ccgccgcggt ggctggcgca cttgagcgca acgatcccgc cgacctacct gcggcggtgg tcaaggtgct gggcaccagc accgagagcg acatagccga actcgccgac ctggccgact cgaccgacct ggccgactcg ggcgccccgc accgcccgcg cgatcccgag ttcgccgccc tggtcaccgc ggcgctggac cagcggccgg gcttcaccct gcgtggtgga accaacgaag tgctgcgcgg tgtcatcgcg cggggattgg gactgt
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMALPRLVPP AGALDSPRAD VLRGEVRAFL AEQSAAGGFT PSVDAWLSAW DEDFTAALAD RGWLGMTVPV EYGGHGRTFL ERFVVTEELL AVGAPVAAHW IADRQIVPSL LKYGTELQKS HFLPRIVRGE CFFGIGMSEP DSGSDLASVR TRAVRGEGGW LLSGTKVWTS GAHRAHAFIV LARTAPVDPD HRHAGLSQFI VDLRGPGVQT RPIISMNGAH HFNEVILDNA HVPDEMVFGA IGNGWTQVTS ELSFERSGPE RFLSTFVLLA ATAESMAEQR FTRDQQLGRL VARIAGLHQM SAAVAGALER NDPADLPAAV VKVLGTSTES DIAELADLAD STDLADSGAP HRPRDPEFAA LVTAALDQRP GFTLRGGTNE VLRGVIARGL GL
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggcgctgccc cggttggtgc cacccgcggg ggccctcgat tccccccggg ccgatgtcct gcgcggcgag gtccgcgcat ttcttgccga gcagtccgcc gccggcgggt tcaccccctc ggttgatgcc tggctgtcgg cgtgggatga ggacttcacc gccgcgctcg ccgaccgcgg ctggttgggc atgaccgtgc ccgtcgagta cggcggacac gggcgcacgt tcctggaacg attcgtcgtc accgaagaac tgctggccgt cggtgcaccc gtggccgccc attggatagc cgaccgtcaa atcgtgccgt cactgctcaa gtacgggacc gaactgcaga agtcgcactt cctgcctcgg attgtgcgcg gtgagtgctt tttcggcatc ggcatgagcg agcccgattc cggttccgat ctggccagcg tgcggacccg ggcggtacgc ggcgagggcg gttggctgtt gtcgggcaca aaggtatgga cctcgggggc acaccgcgcc cacgcgttca tcgtgctcgc gcgcaccgcg ccggtggacc ccgaccacag gcatgccggg ctaagtcaat tcatcgtcga cctacgcggg ccgggggtcc agacccggcc gatcatctcc atgaatggcg cccaccactt caacgaggtg atcctcgaca acgcccatgt acccgacgag atggtgttcg gggcaatcgg caacggctgg acgcaggtga catcggaact cagcttcgag cgcagtggac cagagcgctt cctgtccacc ttcgtattgc tggctgctac cgccgaaagc atggccgagc aacgctttac ccgcgaccag cagctcgggc gactggtggc gcgcatcgcc ggcctgcacc agatgtccgc cgcggtggct ggcgcacttg agcgcaacga tcccgccgac ctacctgcgg cggtggtcaa ggtgctgggc accagcaccg agagcgacat agccgaactc gccgacctgg ccgactcgac cgacctggcc gactcgggcg ccccgcaccg cccgcgcgat cccgagttcg ccgccctggt caccgcggcg ctggaccagc ggccgggctt caccctgcgt ggtggaacca acgaagtgct gcgcggtgtc atcgcgcggg gattgggact gaaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa