MyulA.01613.e

Acyl-CoA dehydrogenase FadE17_2

CENTER ID: MyulA.01613.e
ORGANISM: Mycobacterium ulcerans Agy99
ASSOCIATED DISEASE: Buruli, Bairnsdale ulcer
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyulA.01613.e.A1.GE29936 Full length( MyulA.01613.e ) 1 385
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|362242.7.peg.4482
RefSeq: YP_907401.1
UniProt: A0PUB1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDYDLGDDAR QLRDRLRRLI SEHIPDDFLG ACTTDPQDLA TTESFCKLLA AEDLLALAWP EEHGGGGGSI WQQTVLREEM WARHEPRGPQ YMGINWVGPA IMRYGTPAQK TRHLSAIAAG EVVWCQGFSE PEAGTDLASL RTRAVPEAPD GPGWRITGQK VWTSYAQMAS WCVLAACTHP DARKPKRLTL FLLPMDRPGF TVRPIPSMLG PHHLNEMFLD EVPASPSDVL GEPGDGWRVM REALAFERVG IARYARCESL LDRMRTELGD DWARLPESSR VRWVRALADL RVARLLAYRA VALRDDPTSG AAASAARIVT TTCDQQVAEL LLDVLGPAAL DSGATAPLHG AIEDHWRYAQ AATVASGTIE VQRMLVARDV LGEHR
NT Sequence
ATGGACTACG ACCTCGGCGA TGACGCCCGC CAACTGCGAG ACCGACTGCG TAGGCTGATA TCCGAGCACA TCCCCGACGA CTTCCTCGGT GCGTGCACCA CCGATCCGCA AGATCTCGCG ACCACCGAAT CATTCTGCAA ACTGTTGGCC GCCGAGGATC TGCTGGCACT GGCGTGGCCG GAGGAGCACG GTGGTGGTGG CGGTTCGATC TGGCAGCAGA CCGTGTTGCG CGAGGAGATG TGGGCCCGCC ATGAACCGCG CGGTCCGCAG TACATGGGAA TCAACTGGGT CGGCCCGGCG ATCATGCGCT ACGGAACCCC GGCGCAGAAG ACACGGCACC TGTCCGCAAT CGCGGCCGGC GAGGTGGTGT GGTGCCAAGG ATTCTCCGAG CCTGAGGCGG GAACCGATCT TGCGTCGCTG CGCACCCGTG CGGTACCCGA AGCTCCTGAC GGCCCGGGGT GGCGTATCAC TGGCCAGAAG GTCTGGACGT CATATGCGCA GATGGCGTCC TGGTGTGTGC TGGCGGCCTG CACTCATCCG GACGCGCGCA AACCCAAGCG CCTCACCCTG TTTCTGCTTC CCATGGACCG GCCGGGTTTC ACGGTCCGGC CGATCCCCTC GATGCTGGGA CCGCATCACC TCAACGAGAT GTTCCTGGAC GAGGTGCCGG CGTCGCCCAG CGACGTGTTG GGTGAGCCCG GTGACGGATG GCGAGTGATG CGCGAAGCGC TGGCATTTGA ACGTGTCGGG ATCGCCCGTT ACGCCCGGTG CGAATCATTG CTGGATCGGA TGCGCACCGA GCTCGGCGAC GACTGGGCGC GATTACCCGA GTCGAGTCGG GTCCGCTGGG TGCGGGCACT AGCGGACCTG CGCGTCGCGC GCCTGCTGGC CTACCGCGCG GTCGCGCTGC GCGACGATCC CACCTCGGGT GCCGCGGCAA GCGCCGCCCG CATCGTCACC ACCACCTGCG ATCAACAGGT CGCCGAGTTG CTGTTGGACG TGCTCGGACC GGCCGCGTTG GACAGCGGCG CCACGGCCCC ATTGCACGGG GCGATCGAGG ACCATTGGCG TTACGCACAG GCAGCCACCG TCGCATCCGG CACCATTGAG GTGCAACGAA TGCTCGTTGC ACGTGATGTC TTGGGAGAAC ACCGG
Details for MyulA.01613.e.A1.GE29936
HARVESTED ON: 7/13/2010
SEQUENCED ON: 8/20/2010
EXPECTED MW: 44kDa
OBSERVED MW: 45kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 94
Validated AA Sequence
MDYDLGDDAR QLRDRLRRLI SEHIPDDFLG ACTTDPQDLA TTESFCKLLA AEDLLALAWP EEHGGGGGSI WQQTVLREEM WARHEPRGPQ YMGINWVGPA IMRYGTPAQK TRHLSAIAAG EVVWCQGFSE PEAGTDLASL RTRAVPEAPD GPGWRITGQK VWTSYAQMAS WCVLAACTHP DARKPKRLTL FLLPMDRPGF TVRPIPSMLG PHHLNEMFLD EVPASPSDVL GEPGDGWRVM REALAFERVG IARYARCESL LDRMRTELGD DWARLPESSR VRWVRALADL RVARLLAYRA VALRDDPTSG AAASAARIVT TTCDQQVAEL LLDVLGPAAL DSGATAPLHG AIEDHWRYAQ AATVASGTIE VQRMLVARDV LG
Validated NT Sequence
taccctggaa gctcagaccc agggtcctgg ttcgatggac tacgacctcg gcgatgacgc ccgccaactg cgagaccgac tgcgtaggct gatatccgag cacatccccg acgacttcct cggtgcgtgc accaccgatc cgcaagatct cgcgaccacc gaatcattct gcaaactgtt ggccgccgag gatctgctgg cactggcgtg gccggaggag cacggtggtg gtggcggttc gatctggcag cagaccgtgt tgcgcgagga gatgtgggcc cgccatgaac cgcgcggtcc gcagtacatg ggaatcaact gggtcggccc ggcgatcatg cgctacggaa ccccggcgca gaagacacgg cacctgtccg caatcgcggc cggcgaggtg gtgtggtgcc aaggattctc cgagcctgag gcgggaaccg atcttgcgtc gctgcgcacc cgtgcggtac ccgaagctcc tgacggcccg gggtggcgta tcactggcca gaaggtctgg acgtcatatg cgcagatggc gtcctggtgt gtgctggcgg cctgcactca tccggacgcg cgcaaaccca agcgcctcac cctgtttctg cttcccatgg accggccggg tttcacggtc cggccgatcc cctcgatgct gggaccgcat cacctcaacg agatgttcct ggacgaggtg ccggcgtcgc ccagcgacgt gttgggtgag cccggtgacg gatggcgagt gatgcgcgaa gcgctggcat ttgaacgtgt cgggatcgcc cgttacgccc ggtgcgaatc attgctggat cggatgcgca ccgagctcgg cgacgactgg gcgcgattac ccgagtcgag tcgggtccgc tgggtgcggg cactagcgga cctgcgcgtc gcgcgcctgc tggcctaccg cgcggtcgcg ctgcgcgacg atcccacctc gggtgccgcg gcaagcgccg cccgcatcgt caccaccacc tgcgatcaac aggtcgccga gttgctgttg gacgtgctcg gaccggccgc gttggacagc ggcgccacgg ccccattgca cggggcgatc gaggaccatt ggcgttacgc acaggcagcc accgtcgcat ccggcaccat tgaggtgcaa cgaatgctcg ttgcacgtga tgtcttggga g
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMDYDLGDDA RQLRDRLRRL ISEHIPDDFL GACTTDPQDL ATTESFCKLL AAEDLLALAW PEEHGGGGGS IWQQTVLREE MWARHEPRGP QYMGINWVGP AIMRYGTPAQ KTRHLSAIAA GEVVWCQGFS EPEAGTDLAS LRTRAVPEAP DGPGWRITGQ KVWTSYAQMA SWCVLAACTH PDARKPKRLT LFLLPMDRPG FTVRPIPSML GPHHLNEMFL DEVPASPSDV LGEPGDGWRV MREALAFERV GIARYARCES LLDRMRTELG DDWARLPESS RVRWVRALAD LRVARLLAYR AVALRDDPTS GAAASAARIV TTTCDQQVAE LLLDVLGPAA LDSGATAPLH GAIEDHWRYA QAATVASGTI EVQRMLVARD VLGEH
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggactacgac ctcggcgatg acgcccgcca actgcgagac cgactgcgta ggctgatatc cgagcacatc cccgacgact tcctcggtgc gtgcaccacc gatccgcaag atctcgcgac caccgaatca ttctgcaaac tgttggccgc cgaggatctg ctggcactgg cgtggccgga ggagcacggt ggtggtggcg gttcgatctg gcagcagacc gtgttgcgcg aggagatgtg ggcccgccat gaaccgcgcg gtccgcagta catgggaatc aactgggtcg gcccggcgat catgcgctac ggaaccccgg cgcagaagac acggcacctg tccgcaatcg cggccggcga ggtggtgtgg tgccaaggat tctccgagcc tgaggcggga accgatcttg cgtcgctgcg cacccgtgcg gtacccgaag ctcctgacgg cccggggtgg cgtatcactg gccagaaggt ctggacgtca tatgcgcaga tggcgtcctg gtgtgtgctg gcggcctgca ctcatccgga cgcgcgcaaa cccaagcgcc tcaccctgtt tctgcttccc atggaccggc cgggtttcac ggtccggccg atcccctcga tgctgggacc gcatcacctc aacgagatgt tcctggacga ggtgccggcg tcgcccagcg acgtgttggg tgagcccggt gacggatggc gagtgatgcg cgaagcgctg gcatttgaac gtgtcgggat cgcccgttac gcccggtgcg aatcattgct ggatcggatg cgcaccgagc tcggcgacga ctgggcgcga ttacccgagt cgagtcgggt ccgctgggtg cgggcactag cggacctgcg cgtcgcgcgc ctgctggcct accgcgcggt cgcgctgcgc gacgatccca cctcgggtgc cgcggcaagc gccgcccgca tcgtcaccac cacctgcgat caacaggtcg ccgagttgct gttggacgtg ctcggaccgg ccgcgttgga cagcggcgcc acggccccat tgcacggggc gatcgaggac cattggcgtt acgcacaggc agccaccgtc gcatccggca ccattgaggt gcaacgaatg ctcgttgcac gtgatgtctt gggagaacac aaacagcacg aacaagttct gcagccaagc ttctcgagga tccggctgct aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc ggatatccac aggacgggtg tggtcgccat gatcgcgtag tcgatagtgg ctccaagtag cgaagcgagc aggactgggc ggcggccaaa gcggtcggac agtgctccga gaacgggtgc gcatagaaat tgcatcaacg catatagcgc tagcagcacg ccatagtgac tggcgatgct gtcggaatgg acgatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat gataagctgt caaacatgag aa