MyulA.01613.c

Acyl-CoA dehydrogenase

CENTER ID: MyulA.01613.c
ORGANISM: Mycobacterium ulcerans Agy99
ASSOCIATED DISEASE: Buruli, Bairnsdale ulcer
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyulA.01613.c.A1.GE29934 Full length( MyulA.01613.c ) 1 360
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|362242.7.peg.450
RefSeq: YP_904522.1
UniProt: A0PL32

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MQLTFDADVE AFRDEFISFL DRNLPDQAAG MERPRSCSHV PEWARRWQRL LFDNGWLIPG NPPEFGGRNA SLLQQYVHLQ ELARRRLYRS FNPQGVGIIA ESLLSFGTPE QKRRWAVPIL RAEMTASLGM SEPGAGSDLA SLKTRAVRYR DGEGDRFVVN GQKVWTSGAH DADVLLTFVR TYPDAPKHKG ISALLIPTDT PGVVRRPFAS VCDHDDVDFN EVFFTDAAAP AENLVGELNG GWRVATGSLG HERTMLWLGY ANLLGELATD FTPAGVLQRD HYATLVMDTQ AMRLLGSATL ASAARGQEDV PAQSVLKLLG SEALQRACED ALDSAGGDGL VHPSITAPIA ALNLDNHYGS
NT Sequence
GTGCAGCTGA CCTTTGATGC CGACGTCGAG GCCTTCCGCG ACGAATTCAT CTCGTTTCTG GACCGGAATT TGCCCGATCA GGCGGCCGGG ATGGAGCGGC CGCGGTCCTG CTCGCACGTG CCGGAGTGGG CGCGGCGGTG GCAACGCTTG TTGTTCGACA ACGGGTGGTT GATCCCGGGC AATCCGCCGG AGTTCGGTGG TCGCAATGCC TCACTGCTGC AGCAGTACGT GCATCTGCAG GAGTTGGCCC GGCGCCGCCT TTATCGCAGT TTCAACCCAC AAGGCGTGGG CATCATTGCG GAGTCGCTGC TGTCGTTCGG AACGCCGGAG CAGAAGCGGC GCTGGGCAGT GCCGATCCTG CGGGCGGAGA TGACCGCGTC GTTGGGTATG AGCGAGCCGG GTGCGGGCTC GGATCTGGCG TCGTTGAAGA CGCGGGCAGT GCGCTACCGC GACGGCGAGG GTGACCGTTT TGTGGTCAAT GGGCAGAAGG TGTGGACATC AGGTGCCCAT GACGCCGATG TGCTGCTGAC GTTCGTGCGT ACCTATCCTG ACGCCCCAAA GCACAAGGGA ATCAGCGCTT TGCTGATTCC GACGGACACT CCCGGCGTGG TACGGCGGCC ATTCGCGTCG GTGTGCGACC ACGACGATGT CGACTTCAAT GAGGTGTTTT TCACCGACGC AGCGGCGCCG GCCGAGAACC TGGTGGGTGA GCTCAACGGC GGGTGGCGGG TGGCGACCGG CTCCCTTGGG CACGAACGGA CGATGCTGTG GCTGGGATAT GCCAACCTGT TGGGCGAATT GGCAACCGAT TTCACTCCAG CCGGCGTGCT GCAACGGGAT CACTACGCCA CGTTGGTGAT GGATACCCAG GCGATGCGAC TGCTCGGGTC CGCGACACTG GCCTCGGCGG CGCGTGGACA GGAGGATGTG CCCGCGCAGT CGGTGCTCAA GCTGCTGGGC TCGGAGGCTT TGCAGCGTGC CTGCGAAGAT GCGCTCGACT CGGCAGGCGG CGACGGTCTG GTGCACCCCT CGATCACGGC ACCCATCGCT GCGTTGAACC TGGACAACCA CTATGGCAGC
Details for MyulA.01613.c.A1.GE29934
HARVESTED ON: 7/13/2010
SEQUENCED ON: 8/18/2010
EXPECTED MW: 41kDa
OBSERVED MW: 42kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 93
Validated AA Sequence
MVQLTFDADV EAFRDEFISF LDRNLPDQAA GMERPRSCSH VPEWARRWQR LLFDNGWLIP GNPPEFGGRN ASLLQQYVHL QELARRRLYR SFNPQGVGII AESLLSFGTP EQKRRWAVPI LRAEMTASLG MSEPGAGSDL ASLKTRAVRY RDGEGDRFVV NGQKVWTSGA HDADVLLTFV RTYPDAPKHK GISALLIPTD TPGVVRRPFA SVCDHDDVDF NEVFFTDAAA PAENLVGELN GGWRVATGSL GHERTMLWLG YANLLGELAT DFTPAGVLQR DHYATLVMDT QAMRLLGSAT LASAARGQED VPAQSVLKLL GSEALQRACE DALDSAGGDG LVHPSITAPI AALNLD
Validated NT Sequence
ccctggaagc tcagacccag ggtcctggtt cgatggtgca gctgaccttt gatgccgacg tcgaggcctt ccgcgacgaa ttcatctcgt ttctggaccg gaatttgccc gatcaggcgg ccgggatgga gcggccgcgg tcctgctcgc acgtgccgga gtgggcgcgg cggtggcaac gcttgttgtt cgacaacggg tggttgatcc cgggcaatcc gccggagttc ggtggtcgca atgcctcact gctgcagcag tacgtgcatc tgcaggagtt ggcccggcgc cgcctttatc gcagtttcaa cccacaaggc gtgggcatca ttgcggagtc gctgctgtcg ttcggaacgc cggagcagaa gcggcgctgg gcagtgccga tcctgcgggc ggagatgacc gcgtcgttgg gtatgagcga gccgggtgcg ggctcggatc tggcgtcgtt gaagacgcgg gcagtgcgct accgcgacgg cgagggtgac cgttttgtgg tcaatgggca gaaggtgtgg acatcaggtg cccatgacgc cgatgtgctg ctgacgttcg tgcgtaccta tcctgacgcc ccaaagcaca agggaatcag cgctttgctg attccgacgg acactcccgg cgtggtacgg cggccattcg cgtcggtgtg cgaccacgac gatgtcgact tcaatgaggt gtttttcacc gacgcagcgg cgccggccga gaacctggtg ggtgagctca acggcgggtg gcgggtggcg accggctccc ttgggcacga acggacgatg ctgtggctgg gatatgccaa cctgttgggc gaattggcaa ccgatttcac tccagccggc gtgctgcaac gggatcacta cgccacgttg gtgatggata cccaggcgat gcgactgctc gggtccgcga cactggcctc ggcggcgcgt ggacaggagg atgtgcccgc gcagtcggtg ctcaagctgc tgggctcgga ggctttgcag cgtgcctgcg aagatgcgct cgactcggca ggcggcgacg gtctggtgca cccctcgatc acggcaccca tcgctgcgtt gaacctggac a
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVQLTFDAD VEAFRDEFIS FLDRNLPDQA AGMERPRSCS HVPEWARRWQ RLLFDNGWLI PGNPPEFGGR NASLLQQYVH LQELARRRLY RSFNPQGVGI IAESLLSFGT PEQKRRWAVP ILRAEMTASL GMSEPGAGSD LASLKTRAVR YRDGEGDRFV VNGQKVWTSG AHDADVLLTF VRTYPDAPKH KGISALLIPT DTPGVVRRPF ASVCDHDDVD FNEVFFTDAA APAENLVGEL NGGWRVATGS LGHERTMLWL GYANLLGELA TDFTPAGVLQ RDHYATLVMD TQAMRLLGSA TLASAARGQE DVPAQSVLKL LGSEALQRAC EDALDSAGGD GLVHPSITAP IAALNLDNHY G
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgcagctg acctttgatg ccgacgtcga ggccttccgc gacgaattca tctcgtttct ggaccggaat ttgcccgatc aggcggccgg gatggagcgg ccgcggtcct gctcgcacgt gccggagtgg gcgcggcggt ggcaacgctt gttgttcgac aacgggtggt tgatcccggg caatccgccg gagttcggtg gtcgcaatgc ctcactgctg cagcagtacg tgcatctgca ggagttggcc cggcgccgcc tttatcgcag tttcaaccca caaggcgtgg gcatcattgc ggagtcgctg ctgtcgttcg gaacgccgga gcagaagcgg cgctgggcag tgccgatcct gcgggcggag atgaccgcgt cgttgggtat gagcgagccg ggtgcgggct cggatctggc gtcgttgaag acgcgggcag tgcgctaccg cgacggcgag ggtgaccgtt ttgtggtcaa tgggcagaag gtgtggacat caggtgccca tgacgccgat gtgctgctga cgttcgtgcg tacctatcct gacgccccaa agcacaaggg aatcagcgct ttgctgattc cgacggacac tcccggcgtg gtacggcggc cattcgcgtc ggtgtgcgac cacgacgatg tcgacttcaa tgaggtgttt ttcaccgacg cagcggcgcc ggccgagaac ctggtgggtg agctcaacgg cgggtggcgg gtggcgaccg gctcccttgg gcacgaacgg acgatgctgt ggctgggata tgccaacctg ttgggcgaat tggcaaccga tttcactcca gccggcgtgc tgcaacggga tcactacgcc acgttggtga tggataccca ggcgatgcga ctgctcgggt ccgcgacact ggcctcggcg gcgcgtggac aggaggatgt gcccgcgcag tcggtgctca agctgctggg ctcggaggct ttgcagcgtg cctgcgaaga tgcgctcgac tcggcaggcg gcgacggtct ggtgcacccc tcgatcacgg cacccatcgc tgcgttgaac ctggacaacc actatggcaa acagcacgaa caagttctgc agccaagctt ctcgaggatc cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatccgg atatccacag gacgggtgtg gtcgccatga tcgcgtagtc gatagtggct ccaagtagcg aagcgagcag gactgggcgg cggccaaagc ggtcggacag tgctccgaga acgggtgcgc atagaaattg catcaacgca tatagcgcta gcagcacgcc atagtgactg gcgatgctgt cggaatggac gatatcccgc aagaggcccg gcagtaccgg cataaccaag cctatgccta cagcatccag ggtgacggtg ccgaggatga cgatgagcgc attgttagat ttcatacacg gtgcctgact gcgttagcaa tttaactgtg ataaactacc gcattaaagc ttatcgatga taagctgtca aacatgagaa