MyulA.01613.b

Acyl-CoA dehydrogenase FadE31

CENTER ID: MyulA.01613.b
ORGANISM: Mycobacterium ulcerans Agy99
ASSOCIATED DISEASE: Buruli, Bairnsdale ulcer
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyulA.01613.b.A1.GE29933 Full length( MyulA.01613.b ) 1 377
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|362242.7.peg.4798
RefSeq: YP_907639.1
UniProt: A0PUZ9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MDLDLDEETL AFQGEVREFL AANAEVMPTK SYDNAQGFAQ HRVWDRVLFD AGLSVITWPK QYGGRDAPLL HWVIFEEEYF RAGAPGRASA NGTSMLAPTL FAHGTAEQLD RILLKMASGE QIWAQTWSEP ESGSDLASLR STATKTDGGW LLNGQKIWSS RAPFADMGFG LFRSDPSAQR HRGLTYFMFD LKAKGITVRP IVQLGGDTGF GEIFLDDVFV PDQDVIGAPN DGWRAAMSTS SNERGMSLRS PARFMAAAQK LVQLWKDAGS PPEFANRVAD AWIKAQAYRL QTFGTVTRLA AGGELGAGSS VTKVFWSDLD VDIHQTALDI RGADAELVSP WTEGLLFALG GPIYAGTNEI QRNIISERLL GLPREKK
NT Sequence
ATGGATTTGG ATCTTGACGA AGAGACCCTG GCTTTCCAGG GCGAAGTGCG CGAGTTCCTG GCGGCCAACG CCGAGGTCAT GCCGACCAAG TCATACGACA ATGCGCAAGG CTTCGCCCAG CATCGAGTTT GGGACCGAGT GCTCTTCGAC GCGGGCCTGT CGGTGATCAC CTGGCCGAAA CAGTACGGCG GCCGCGACGC GCCCCTGCTG CACTGGGTGA TCTTCGAAGA GGAGTACTTC CGCGCCGGAG CACCGGGCCG GGCCAGCGCC AACGGTACCT CGATGCTGGC CCCCACCCTG TTCGCGCACG GTACCGCCGA GCAGCTAGAC CGCATCCTGC TCAAAATGGC CAGTGGCGAA CAGATCTGGG CACAAACCTG GTCGGAGCCC GAATCCGGCA GCGATCTGGC GTCACTGCGA TCCACTGCCA CCAAGACCGA CGGAGGCTGG CTACTCAACG GCCAGAAGAT CTGGAGCTCG CGCGCACCAT TCGCCGACAT GGGATTCGGA CTGTTCCGCT CCGACCCGTC AGCACAGCGC CATCGTGGGC TGACGTACTT CATGTTCGAC CTGAAGGCCA AAGGGATTAC CGTTCGCCCG ATCGTCCAAC TCGGCGGCGA CACCGGCTTT GGTGAAATCT TCCTCGATGA CGTGTTCGTG CCCGACCAGG ACGTCATCGG TGCACCCAAT GACGGTTGGC GCGCGGCGAT GAGCACGTCG AGCAACGAGC GAGGCATGTC GCTGCGCAGT CCGGCTCGCT TCATGGCGGC TGCCCAGAAG CTGGTTCAGC TGTGGAAGGA CGCCGGCTCG CCCCCCGAGT TCGCCAACCG AGTGGCCGAT GCCTGGATCA AGGCGCAGGC CTATCGGCTG CAGACCTTCG GCACCGTGAC CAGGCTGGCG GCCGGCGGTG AGTTGGGCGC CGGATCGTCG GTAACCAAGG TGTTCTGGTC CGACCTGGAC GTGGATATCC ATCAGACCGC GCTCGACATC CGCGGCGCCG ACGCCGAGTT GGTCAGCCCG TGGACCGAAG GTCTGCTCTT TGCCCTTGGT GGTCCAATTT ATGCCGGTAC CAATGAAATC CAGCGCAATA TCATTTCGGA GCGGCTGCTG GGCCTGCCAC GAGAGAAGAA G
Details for MyulA.01613.b.A1.GE29933
HARVESTED ON: 7/13/2010
SEQUENCED ON: 8/18/2010
EXPECTED MW: 43kDa
OBSERVED MW: 43kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 100
PERCENT COVERAGE: 94
Validated AA Sequence
MDLDLDEETL AFQGEVREFL AANAEVMPTK SYDNAQGFAQ HRVWDRVLFD AGLSVITWPK QYGGRDAPLL HWVIFEEEYF RAGAPGRASA NGTSMLAPTL FAHGTAEQLD RILLKMASGE QIWAQTWSEP ESGSDLASLR STATKTDGGW LLNGQKIWSS RAPFADMGFG LFRSDPSAQR HRGLTYFMFD LKAKGITVRP IVQLGGDTGF GEIFLDDVFV PDQDVIGAPN DGWRAAMSTS SNERGMSLRS PARFMAAAQK LVQLWKDAGS PPEFANRVAD AWIKAQAYRL QTFGTVTRLA AGGELGAGSS VTKVFWSDLD VDIHQTALDI RGADAELVSP WTEGLLFALG GPIYAGTNEI QRNIISERLL GLPR
Validated NT Sequence
ctggaagctc agacccaggg tcctggttcg atggatttgg atcttgacga agagaccctg gctttccagg gcgaagtgcg cgagttcctg gcggccaacg ccgaggtcat gccgaccaag tcatacgaca atgcgcaagg cttcgcccag catcgagttt gggaccgagt gctcttcgac gcgggcctgt cggtgatcac ctggccgaaa cagtacggcg gccgcgacgc gcccctgctg cactgggtga tcttcgaaga ggagtacttc cgcgccggag caccgggccg ggccagcgcc aacggtacct cgatgctggc ccccaccctg ttcgcgcacg gtaccgccga gcagctagac cgcatcctgc tcaaaatggc cagtggcgaa cagatctggg cacaaacctg gtcggagccc gaatccggca gcgatctggc gtcactgcga tccactgcca ccaagaccga cggaggctgg ctactcaacg gccagaagat ctggagctcg cgcgcaccat tcgccgacat gggattcgga ctgttccgct ccgacccgtc agcacagcgc catcgtgggc tgacgtactt catgttcgac ctgaaggcca aagggattac cgttcgcccg atcgtccaac tcggcggcga caccggcttt ggtgaaatct tcctcgatga cgtgttcgtg cccgaccagg acgtcatcgg tgcacccaat gacggttggc gcgcggcgat gagcacgtcg agcaacgagc gaggcatgtc gctgcgcagt ccggctcgct tcatggcggc tgcccagaag ctggttcagc tgtggaagga cgccggctcg ccccccgagt tcgccaaccg agtggccgat gcctggatca aggcgcaggc ctatcggctg cagaccttcg gcaccgtgac caggctggcg gccggcggtg agttgggcgc cggatcgtcg gtaaccaagg tgttctggtc cgacctggac gtggatatcc atcagaccgc gctcgacatc cgcggcgccg acgccgagtt ggtcagcccg tggaccgaag gtctgctctt tgcccttggt ggtccaattt atgccggtac caatgaaatc cagcgcaata tcatttcgga gcggctgctg ggcctgccac gag
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMDLDLDEET LAFQGEVREF LAANAEVMPT KSYDNAQGFA QHRVWDRVLF DAGLSVITWP KQYGGRDAPL LHWVIFEEEY FRAGAPGRAS ANGTSMLAPT LFAHGTAEQL DRILLKMASG EQIWAQTWSE PESGSDLASL RSTATKTDGG WLLNGQKIWS SRAPFADMGF GLFRSDPSAQ RHRGLTYFMF DLKAKGITVR PIVQLGGDTG FGEIFLDDVF VPDQDVIGAP NDGWRAAMST SSNERGMSLR SPARFMAAAQ KLVQLWKDAG SPPEFANRVA DAWIKAQAYR LQTFGTVTRL AAGGELGAGS SVTKVFWSDL DVDIHQTALD IRGADAELVS PWTEGLLFAL GGPIYAGTNE IQRNIISERL LGLPREK
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggatttggat cttgacgaag agaccctggc tttccagggc gaagtgcgcg agttcctggc ggccaacgcc gaggtcatgc cgaccaagtc atacgacaat gcgcaaggct tcgcccagca tcgagtttgg gaccgagtgc tcttcgacgc gggcctgtcg gtgatcacct ggccgaaaca gtacggcggc cgcgacgcgc ccctgctgca ctgggtgatc ttcgaagagg agtacttccg cgccggagca ccgggccggg ccagcgccaa cggtacctcg atgctggccc ccaccctgtt cgcgcacggt accgccgagc agctagaccg catcctgctc aaaatggcca gtggcgaaca gatctgggca caaacctggt cggagcccga atccggcagc gatctggcgt cactgcgatc cactgccacc aagaccgacg gaggctggct actcaacggc cagaagatct ggagctcgcg cgcaccattc gccgacatgg gattcggact gttccgctcc gacccgtcag cacagcgcca tcgtgggctg acgtacttca tgttcgacct gaaggccaaa gggattaccg ttcgcccgat cgtccaactc ggcggcgaca ccggctttgg tgaaatcttc ctcgatgacg tgttcgtgcc cgaccaggac gtcatcggtg cacccaatga cggttggcgc gcggcgatga gcacgtcgag caacgagcga ggcatgtcgc tgcgcagtcc ggctcgcttc atggcggctg cccagaagct ggttcagctg tggaaggacg ccggctcgcc ccccgagttc gccaaccgag tggccgatgc ctggatcaag gcgcaggcct atcggctgca gaccttcggc accgtgacca ggctggcggc cggcggtgag ttgggcgccg gatcgtcggt aaccaaggtg ttctggtccg acctggacgt ggatatccat cagaccgcgc tcgacatccg cggcgccgac gccgagttgg tcagcccgtg gaccgaaggt ctgctctttg cccttggtgg tccaatttat gccggtacca atgaaatcca gcgcaatatc atttcggagc ggctgctggg cctgccacga gagaagaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa