MyulA.01613.a

Acyl-CoA dehydrogenase FadE30

CENTER ID: MyulA.01613.a
ORGANISM: Mycobacterium ulcerans Agy99
ASSOCIATED DISEASE: Buruli, Bairnsdale ulcer
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyulA.01613.a.A1.GE29932 Full length( MyulA.01613.a ) 1 382
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|362242.7.peg.4796
RefSeq: YP_907637.1
UniProt: A0PUZ7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MQDVEEFRAE VRGWLAANLV GEFATLKGLG GPGREHEAFE ERRAWNQHLA AAGLTCLGWP VEHGGRGLST AHRVAFYEEY ARADAPDKVN HFGEELLGPT LIAFGTPEQQ QRFLPRILDV TELWCQGYSE PGAGSDLANV ATTAELDGDQ WVINGQKVWT SLAHLSQWCF VVARTEKGSK RHAGLSYLLV PLDQPSVQIR PIVQITGTAE FNEVFFDDAR TDADLVVGAP GDGWRVAMGT LTFERGVSTL GQQIVYAREL SDLAALARRT GAADDPFIRE HLTRAWTGLR AMRSYALATM DVEQPGQDNV SKLLWANWHR SLGELAMDVI GKPGLTLADG EFDEWQRLFL FTRADTIYGG SNEIQRNIIA ERVLGLPREA KG
NT Sequence
ATGCAGGACG TCGAGGAGTT TCGGGCGGAG GTCCGCGGCT GGCTCGCGGC CAATCTGGTC GGAGAATTCG CAACGCTCAA GGGGCTCGGC GGGCCGGGAC GTGAACACGA GGCTTTCGAG GAACGCCGGG CCTGGAACCA GCATCTGGCC GCGGCCGGGC TGACCTGCCT GGGGTGGCCG GTCGAGCATG GCGGCCGGGG GCTCTCGACC GCCCACCGCG TGGCTTTCTA CGAGGAATAC GCGCGCGCGG ATGCGCCGGA CAAGGTCAAC CACTTCGGTG AAGAGCTGCT CGGGCCAACG CTGATCGCCT TCGGGACACC CGAACAACAG CAGCGCTTCC TGCCGCGCAT CCTCGACGTC ACCGAGCTGT GGTGCCAGGG GTATTCCGAG CCCGGCGCCG GTAGTGACCT GGCCAATGTG GCGACCACCG CCGAACTCGA CGGTGATCAA TGGGTAATCA ACGGCCAGAA GGTATGGACG TCGCTGGCTC ATCTTTCGCA GTGGTGCTTC GTGGTCGCGC GAACCGAGAA GGGCTCCAAA CGCCATGCCG GGCTGTCGTA TCTGCTGGTC CCGCTGGATC AGCCCAGCGT GCAGATCCGG CCGATCGTCC AGATCACCGG TACCGCGGAG TTCAATGAGG TGTTCTTCGA CGACGCCCGC ACCGATGCTG ACTTGGTGGT CGGCGCACCG GGTGACGGCT GGCGCGTCGC GATGGGCACG CTGACCTTCG AGCGGGGCGT CTCGACGCTG GGGCAGCAGA TCGTCTATGC CCGTGAGCTT TCCGATCTGG CCGCTCTCGC GCGGCGCACC GGCGCCGCCG ACGATCCGTT CATCCGGGAA CACCTGACCC GGGCGTGGAC CGGACTACGG GCAATGCGTT CGTATGCCCT GGCCACCATG GATGTTGAGC AGCCCGGCCA GGACAACGTT TCGAAGTTGC TGTGGGCCAA CTGGCATCGC AGTCTCGGCG AACTGGCGAT GGATGTGATC GGCAAGCCGG GCTTGACGCT GGCCGACGGC GAGTTCGATG AATGGCAGCG GCTGTTCTTG TTCACCCGCG CTGACACGAT CTACGGCGGA TCCAACGAGA TTCAGCGCAA CATCATTGCC GAGCGGGTGC TCGGCCTGCC CCGGGAGGCC AAGGGA
Details for MyulA.01613.a.A1.GE29932
HARVESTED ON: 7/13/2010
SEQUENCED ON: 8/20/2010
EXPECTED MW: 44kDa
OBSERVED MW: 44kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Few (1-10)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 78
PERCENT COVERAGE: 57
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMQDVEEFRA EVRGWLAANL VGEFATLKGL GGPGREHEAF EERRAWNQHL AAAGLTCLGW PVEHGGRGLS TAHRVAFYXE YARADAPDKV NHFGEXLLGP TLIAFGTPEQ QQRFLPRILX XXXXWCXGYS XPGXGSDLAN VXTXXKLXGD QXVINGXKVW TSLAHXXAVV XXGRAXREGL QTXXRAVXSA GXXGSAQRAD PXDRPDHRXR GVQ
Validated NT Sequence
ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcaggacgtc gaggagtttc gggcggaggt ccgcggctgg ctcgcggcca atctggtcgg agaattcgca acgctcaagg ggctcggcgg gccgggacgt gaacacgagg ctttcgagga acgccgggcc tggaaccagc atctggccgc ggccgggctg acctgcctgg ggtggccggt cgagcatggc ggccgggggc tctcgaccgc ccaccgcgtg gctttctacn aggaatacgc gcgcgcggat gcgccggaca aggtcaacca cttcggtgaa nagctgctcg ggccaacnct gatcgccttc gggacacccg aacaacagca gcgcttcctg ccgcgcatcc tcnacntcnc nnanntgtgg tgccnggggt attccnancc cggcnccggt agtgacctgg ccaatgtgnc nacnncnncc aaactcnncg gtgatcaang ggtaatcaac ggccanaagg tatggacgtc gctggctcat cnttnngcag tggtgnnnng tggtcgngcg anccgagaag ggctncaaac gncannncgg gctgtnnnnt ctgctggtnc cgntggatca gcccagcgtg cagatccgnc cgatcgtcca gatcaccggt nncgcggagt tcaatgaggt gttnttcgac gacgcccgca ccgatgctga cttggtggtc ggcgcaccgg gtgacggctg gcgcgtcgcg atgggcacgn tgaccttcga gcggggcgtc tcgacgctgg ggcagcagat cgtctatgcc cgtgagcttt ccgatctggc cgctctcgcg cggcgcaccg gcgccgccga cgatccgttc atccgggaac acctgacccg ggcgtggacc ggactacggg caatgcgttc gtatgccctg gccaccatgg atgttgagca gcccggccag gacaacgttt cgaagttgct gtgggccaac tggcatngca gtctcggcga actggcgatg gatgtgatcg gcaagccggg cttgacgctg gccgacggcg agttcgatga atggcagcgg ctgttcttgt tcacccgcgc tgacacgatc tacggcggat ccaacgagat tcagcgcaac atcattgccg agcgggtgct cggcctgccc cgggaggcca agtaataa
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMQDVEEFRA EVRGWLAANL VGEFATLKGL GGPGREHEAF EERRAWNQHL AAAGLTCLGW PVEHGGRGLS TAHRVAFYEE YARADAPDKV NHFGEELLGP TLIAFGTPEQ QQRFLPRILD VTELWCQGYS EPGAGSDLAN VATTAELDGD QWVINGQKVW TSLAHLSQWC FVVARTEKGS KRHAGLSYLL VPLDQPSVQI RPIVQITGTA EFNEVFFDDA RTDADLVVGA PGDGWRVAMG TLTFERGVST LGQQIVYARE LSDLAALARR TGAADDPFIR EHLTRAWTGL RAMRSYALAT MDVEQPGQDN VSKLLWANWH RSLGELAMDV IGKPGLTLAD GEFDEWQRLF LFTRADTIYG GSNEIQRNII AERVLGLPRE AK
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcaggacgtc gaggagtttc gggcggaggt ccgcggctgg ctcgcggcca atctggtcgg agaattcgca acgctcaagg ggctcggcgg gccgggacgt gaacacgagg ctttcgagga acgccgggcc tggaaccagc atctggccgc ggccgggctg acctgcctgg ggtggccggt cgagcatggc ggccgggggc tctcgaccgc ccaccgcgtg gctttctacg aggaatacgc gcgcgcggat gcgccggaca aggtcaacca cttcggtgaa gagctgctcg ggccaacgct gatcgccttc gggacacccg aacaacagca gcgcttcctg ccgcgcatcc tcgacgtcac cgagctgtgg tgccaggggt attccgagcc cggcgccggt agtgacctgg ccaatgtggc gaccaccgcc gaactcgacg gtgatcaatg ggtaatcaac ggccagaagg tatggacgtc gctggctcat ctttcgcagt ggtgcttcgt ggtcgcgcga accgagaagg gctccaaacg ccatgccggg ctgtcgtatc tgctggtccc gctggatcag cccagcgtgc agatccggcc gatcgtccag atcaccggta ccgcggagtt caatgaggtg ttcttcgacg acgcccgcac cgatgctgac ttggtggtcg gcgcaccggg tgacggctgg cgcgtcgcga tgggcacgct gaccttcgag cggggcgtct cgacgctggg gcagcagatc gtctatgccc gtgagctttc cgatctggcc gctctcgcgc ggcgcaccgg cgccgccgac gatccgttca tccgggaaca cctgacccgg gcgtggaccg gactacgggc aatgcgttcg tatgccctgg ccaccatgga tgttgagcag cccggccagg acaacgtttc gaagttgctg tgggccaact ggcatcgcag tctcggcgaa ctggcgatgg atgtgatcgg caagccgggc ttgacgctgg ccgacggcga gttcgatgaa tggcagcggc tgttcttgtt cacccgcgct gacacgatct acggcggatc caacgagatt cagcgcaaca tcattgccga gcgggtgctc ggcctgcccc gggaggccaa gaaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa