MyulA.01582.b

Short-chain type dehydrogenase/reductase

CENTER ID: MyulA.01582.b
ORGANISM: Mycobacterium ulcerans Agy99
ASSOCIATED DISEASE: Buruli, Bairnsdale ulcer
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyulA.01582.b.A1.GE29906 Full length( MyulA.01582.b ) 1 297
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|362242.7.peg.3622
RefSeq: YP_906818.1
UniProt: A0PSM8

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTDRDLLAGR GVVVVGGSRG IGRAVAELLA RLGAGVVISG REPDAVRDAT EAVISAGGRA AGIAGAADHE DTANALVDRC MTSFGRLDAL INCAGIAEPP GSSILNISPA QSDGLLRAHL GTVFHACRVA APVLAAQGHG SIINTGSVAF LGDYGGSGYP AGKAAVNGLT LAISAELKAR GVRANVICPG ARTRLSSGPN YEAHINELHR RGLLDEMTRQ ASLDAPPADF VAPLYAYLAS DLASGITGQI FVASGGSVGT FDRPTPRVLG WRDHRADPPW PVEDLHTMIN ARLLDTD
NT Sequence
ATGACCGACC GAGACCTCCT GGCCGGACGG GGCGTTGTCG TGGTCGGCGG CAGCCGCGGC ATCGGACGCG CCGTCGCCGA ACTGCTGGCC CGACTCGGTG CCGGCGTGGT GATCAGTGGA CGCGAGCCGG ACGCGGTACG TGACGCAACC GAGGCGGTTA TCTCCGCGGG CGGTCGCGCG GCCGGCATCG CCGGCGCGGC GGACCACGAA GACACGGCAA ATGCACTGGT CGATCGGTGC ATGACCAGCT TCGGACGACT AGACGCCTTG ATCAACTGTG CGGGCATCGC CGAGCCGCCC GGCTCCTCGA TCCTCAACAT CAGTCCGGCT CAATCCGATG GTCTGCTTCG AGCCCATCTG GGCACCGTAT TCCACGCCTG CCGGGTCGCC GCGCCGGTGC TGGCCGCTCA GGGACACGGA TCGATCATCA ACACGGGTTC GGTTGCGTTC CTCGGCGACT ACGGGGGCAG CGGATATCCC GCGGGCAAAG CGGCCGTCAA CGGGCTGACC CTGGCAATAT CCGCAGAACT GAAAGCGCGC GGTGTACGTG CCAACGTCAT CTGCCCCGGG GCGCGTACGC GGCTGTCCAG CGGCCCGAAC TATGAAGCGC ATATCAACGA ACTCCATCGC CGTGGACTGC TCGACGAGAT GACTAGGCAG GCCTCCCTCG ATGCGCCGCC GGCCGATTTC GTCGCGCCGC TGTATGCTTA TTTGGCCAGC GATCTGGCCA GCGGGATCAC GGGTCAGATC TTCGTTGCTT CAGGAGGATC CGTTGGCACC TTCGACCGTC CGACGCCACG GGTCCTGGGC TGGCGCGATC ATCGGGCCGA TCCGCCGTGG CCGGTCGAGG ACCTGCACAC CATGATCAAC GCCCGGCTGC TCGACACGGA C
Details for MyulA.01582.b.A1.GE29906
HARVESTED ON: 7/20/2010
SEQUENCED ON: 8/2/2010
EXPECTED MW: 32kDa
OBSERVED MW: 32kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 76
PERCENT COVERAGE: 106
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMTDRDLLAG RGVVVVGGSR GIGRAVAELL ARLGAGVVIS GREPDAVRDA TEAVISAGGR AAGIAGAADH EDTANALVDR CMTSFGRLDA LINCAGIAEP PGSSILNISP AQSDGLLRAH LGTVFHACRX AAPXLAAXGH GSIINTGSVA FLGDYGGSXY PAGKAAVNGL TLXISAXLKA RGVRAXVXCP GARTRLSSGP NYXXHIXELX RRXLLDXXTX XASXDAXPAD FVAXCMXYLX SDLXXXSRXX XSLXQXXXXX LRRRXXXXGX XXXXXXPXXX XXXXXAXXXX IXXXXXXXXX XXXXXXXXXX XCXXXXXX
Validated NT Sequence
annnattttn nttantttaa gaaggagann taccatggct catcaccatc accatcatat gggtaccctg gaagctcaga cccagggtcc tggttcgatg accgaccgag acctcctggc cggacggggc gttgtcgtgg tcggcggcag ccgcggcatc ggacgcgccg tcgccgaact gctggcccga ctcggtgccg gcgtggtgat cagtggacgc gagccggacg cggtacgtga cgcaaccgag gcggttatct ccgcgggcgg tcgcgcggcc ggcatcgccg gcgcggcgga ccacgaagac acggcaaatg cactggtcga tcggtgcatg accagcttcg gacgactaga cgccttgatc aactgtgcgg gcatcgccga gccgcccggc tcctcgatcc tcaacatcag tccggctcaa tccgatggtc tgcttcgagc ccatctgggc accgtattcc acgcctgccg ggncgccgcg ccggngctgg ccgctcangg acacggatcg atcatcaaca cgggttcggt tgcgttcctc ggcgactacg ggggcagcgn atatcccgcg ggcaaagcgg ccgtcaacgg gctgaccctg gnaatatccg canaactgaa agcgcgcggt gtacgtgccn acgtcntctg cccnggggcg cgtacgcggc tgtccagcgg cccnaactat gannngcata tcancgaact ccntcgccgt gnactgctcg acnannngac tannnangcc tccntcgatg cgcnnccngc cgatttcgtc gcgcnntgta tgnnttattt gnccagcgat ctggncagng nntcacggnt nannncntcg ttgnntcagg anganncnnn gnnncttcga cgtcgannca nnnntnnngg gntgnnnnat cnncngnccn nncccnagnn nnnnnnnnng nnnnnnnnng ctgnncnnnn nnnnataaan nnnnnnntnn ngnannnnnn tnnnnnanga ncngntnnnn ananntnann nnnnngntgc nccnnnnnnn nnnnnnnnna
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTDRDLLAG RGVVVVGGSR GIGRAVAELL ARLGAGVVIS GREPDAVRDA TEAVISAGGR AAGIAGAADH EDTANALVDR CMTSFGRLDA LINCAGIAEP PGSSILNISP AQSDGLLRAH LGTVFHACRV AAPVLAAQGH GSIINTGSVA FLGDYGGSGY PAGKAAVNGL TLAISAELKA RGVRANVICP GARTRLSSGP NYEAHINELH RRGLLDEMTR QASLDAPPAD FVAPLYAYLA SDLASGITGQ IFVASGGSVG TFDRPTPRVL GWRDHRADPP WPVEDLHTMI NARLLDT
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaccgaccga gacctcctgg ccggacgggg cgttgtcgtg gtcggcggca gccgcggcat cggacgcgcc gtcgccgaac tgctggcccg actcggtgcc ggcgtggtga tcagtggacg cgagccggac gcggtacgtg acgcaaccga ggcggttatc tccgcgggcg gtcgcgcggc cggcatcgcc ggcgcggcgg accacgaaga cacggcaaat gcactggtcg atcggtgcat gaccagcttc ggacgactag acgccttgat caactgtgcg ggcatcgccg agccgcccgg ctcctcgatc ctcaacatca gtccggctca atccgatggt ctgcttcgag cccatctggg caccgtattc cacgcctgcc gggtcgccgc gccggtgctg gccgctcagg gacacggatc gatcatcaac acgggttcgg ttgcgttcct cggcgactac gggggcagcg gatatcccgc gggcaaagcg gccgtcaacg ggctgaccct ggcaatatcc gcagaactga aagcgcgcgg tgtacgtgcc aacgtcatct gccccggggc gcgtacgcgg ctgtccagcg gcccgaacta tgaagcgcat atcaacgaac tccatcgccg tggactgctc gacgagatga ctaggcaggc ctccctcgat gcgccgccgg ccgatttcgt cgcgccgctg tatgcttatt tggccagcga tctggccagc gggatcacgg gtcagatctt cgttgcttca ggaggatccg ttggcacctt cgaccgtccg acgccacggg tcctgggctg gcgcgatcat cgggccgatc cgccgtggcc ggtcgaggac ctgcacacca tgatcaacgc ccggctgctc gacacgaaac agcacgaaca agttctgcag ccaagcttct cgaggatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa aggaggaact atatccggat atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt atcgatgata agctgtcaaa catgagaa