MyulA.01530.e

Enoyl-CoA hydratase EchA10_1

CENTER ID: MyulA.01530.e
ORGANISM: Mycobacterium ulcerans Agy99
ASSOCIATED DISEASE: Buruli, Bairnsdale ulcer
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyulA.01530.e.A1.GE29899 Full length( MyulA.01530.e ) 1 272
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|362242.7.peg.4533
RefSeq: YP_907435.1
UniProt: A0PUE5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSLEADSAGP EGSVTSRRDG TLLRITLDRP SRRNSLTRNM IEMLLDTLGG AATDDSLRAI LIRGAAADFC AGADWVAAST GTQRHRTGDL LRRIPHAAHR VIELLHTIHL PVVCGVQGWA AGLGANLALA ADFTVATSDA TFWEPFVVRG FSPDSGSTWL LPRLVGLARA RRMLLLGEQV TAPEAADWGL IHQVVDATDL DSAVDELLTR LARGPTVAIG LAKQALNHGQ HATLTESMTH ELFNLELTCR TSDFKEGLTA FRQHRTPKFE GR
NT Sequence
GTGAGTCTCG AGGCCGACTC GGCCGGGCCC GAGGGGTCGG TGACATCGCG CCGCGATGGC ACCCTGCTGC GAATCACGCT CGATCGACCT TCACGGCGTA ATTCGTTGAC TCGGAACATG ATCGAGATGT TGCTCGATAC GCTGGGCGGC GCCGCCACTG ACGACTCGCT GCGTGCGATT CTGATCCGGG GTGCCGCTGC CGATTTCTGC GCCGGCGCCG ATTGGGTCGC GGCCAGCACC GGCACCCAAC GCCATCGCAC CGGCGACCTG CTAAGGCGAA TTCCGCACGC CGCGCACCGC GTCATCGAAC TCCTACACAC CATCCACCTC CCGGTCGTGT GCGGTGTCCA AGGGTGGGCC GCCGGGCTGG GCGCCAACCT GGCGCTCGCC GCCGACTTCA CCGTGGCCAC CAGTGATGCC ACGTTCTGGG AACCTTTCGT AGTCCGCGGG TTCAGCCCGG ACTCGGGATC TACCTGGCTG CTGCCGCGCC TGGTTGGCTT GGCCCGGGCC CGGCGGATGC TGCTGCTTGG CGAGCAGGTG ACCGCGCCCG AGGCAGCCGA CTGGGGCTTG ATCCACCAAG TCGTCGATGC GACGGACCTC GACAGCGCAG TCGACGAGCT GCTCACCCGA CTGGCTCGCG GGCCGACTGT TGCGATCGGG CTTGCCAAGC AGGCCCTGAA CCACGGCCAG CACGCAACCC TGACCGAGTC GATGACACAT GAACTGTTCA ACCTCGAACT AACTTGTCGG ACCTCCGATT TCAAAGAGGG CTTGACCGCA TTCCGGCAAC ATCGCACGCC GAAATTCGAG GGACGA
Details for MyulA.01530.e.A1.GE29899
HARVESTED ON: 7/20/2010
SEQUENCED ON: 8/2/2010
EXPECTED MW: 31kDa
OBSERVED MW: 31kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 95
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMVSLEADSA GPEGSVTSRR DGTLLRITLD RPSRRNSLTR NMIEMLLDTL GGAATDDSLR AILIRGAAAD FCAGADWVAA STGTQRHRTG DLLRRIPHAA HRVIELLHTI HLPVVCGVQG WAAGLGANLA LAADFTVATS DATFWEPFVV RGFSPDSGST WLLPRLVGLA RARRMLLLGE QVTAPXAADW GLIHQVVDAT DLDSAVDELL TRLARGPTVA IGLAXXALNH GQHATLTESM THELFNLELT CRTSXFKRXX XHSGXIARRN SRGX
Validated NT Sequence
tannnnannt ttntttantt taagaaggag anataccatg gctcatcacc atcaccatca tatgggtacc ctggaagctc agacccaggg tcctggttcg atggtgagtc tcgaggccga ctcggccggg cccgaggggt cggtgacatc gcgccgcgat ggcaccctgc tgcgaatcac gctcgatcga ccttcacggc gtaattcgtt gactcggaac atgatcgaga tgttgctcga tacgctgggc ggcgccgcca ctgacgactc gctgcgtgcg attctgatcc ggggtgccgc tgccgatttc tgcgccggcg ccgattgggt cgcggccagc accggcaccc aacgccatcg caccggcgac ctgctaaggc gaattccgca cgccgcgcac cgcgtcatcg aactcctaca caccatccac ctcccggtcg tgtgcggtgt ccaagggtgg gccgccgggc tgggcgccaa cctggcgctc gccgccgact tcaccgtggc caccagtgat gccacgttct gggaaccttt cgtagtccgc gggttcagcc cggactcggg atctacctgg ctgctgccgc gcctggttgg cttggcccgg gcccggcgga tgctgctgct tggcgagcag gtgaccgcgc ccgangcagc cgactggggc ttgatccacc aagtcgtcga tgcgacggac ctcgacagcg cagtcgacga gctgctcacc cgactggctc gcgggccgac tgttgcgatc gggcttgccn agcangccct gaaccacggn cagcacgcaa ccctgaccga gtcnatgaca catgaactgt tcaacctcga actaacttgt cggacctccg anttcaagag ggnttgnncg cattccggca ncatcgcacg ccgaaattcg agggganaat aaacagcacg nacaagttct gcagccnanc ttctcnaggn tnccggctgc taannnnccc naaaggaagc tganntggct gctgccnccn ctnnnnnnnn actnnnaann a
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVSLEADSA GPEGSVTSRR DGTLLRITLD RPSRRNSLTR NMIEMLLDTL GGAATDDSLR AILIRGAAAD FCAGADWVAA STGTQRHRTG DLLRRIPHAA HRVIELLHTI HLPVVCGVQG WAAGLGANLA LAADFTVATS DATFWEPFVV RGFSPDSGST WLLPRLVGLA RARRMLLLGE QVTAPEAADW GLIHQVVDAT DLDSAVDELL TRLARGPTVA IGLAKQALNH GQHATLTESM THELFNLELT CRTSDFKEGL TAFRQHRTPK FEG
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgagtctc gaggccgact cggccgggcc cgaggggtcg gtgacatcgc gccgcgatgg caccctgctg cgaatcacgc tcgatcgacc ttcacggcgt aattcgttga ctcggaacat gatcgagatg ttgctcgata cgctgggcgg cgccgccact gacgactcgc tgcgtgcgat tctgatccgg ggtgccgctg ccgatttctg cgccggcgcc gattgggtcg cggccagcac cggcacccaa cgccatcgca ccggcgacct gctaaggcga attccgcacg ccgcgcaccg cgtcatcgaa ctcctacaca ccatccacct cccggtcgtg tgcggtgtcc aagggtgggc cgccgggctg ggcgccaacc tggcgctcgc cgccgacttc accgtggcca ccagtgatgc cacgttctgg gaacctttcg tagtccgcgg gttcagcccg gactcgggat ctacctggct gctgccgcgc ctggttggct tggcccgggc ccggcggatg ctgctgcttg gcgagcaggt gaccgcgccc gaggcagccg actggggctt gatccaccaa gtcgtcgatg cgacggacct cgacagcgca gtcgacgagc tgctcacccg actggctcgc gggccgactg ttgcgatcgg gcttgccaag caggccctga accacggcca gcacgcaacc ctgaccgagt cgatgacaca tgaactgttc aacctcgaac taacttgtcg gacctccgat ttcaaagagg gcttgaccgc attccggcaa catcgcacgc cgaaattcga gggaaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa