MyulA.01370.a

Phosphomannomutase PmmA

CENTER ID: MyulA.01370.a
ORGANISM: Mycobacterium ulcerans Agy99
ASSOCIATED DISEASE: Buruli, Bairnsdale ulcer
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyulA.01370.a.A1.GE29889 Full length( MyulA.01370.a ) 1 466
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyulA.01370.a.A1.PS00791 Full length( MyulA.01370.a ) 1 466

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|362242.7.peg.3050
RefSeq: YP_906404.1
UniProt: A0PRG4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSWPAAVINR VIKAYDVRGL VGEEITEALC ADIGAAFARL MRGEGARQVV IGYDMRDSSP ALAAAFASGV VAQGLGVVRI GLGSTDQLYF AAGQLDCPGA MFTASHNPAA YNGIKLCRAG AKPVGADTGL NAIREDLVNP VAIAVAETAG TIADKDVLGD YGVFLRSLVN TADLRPLRVA VDAGNGMAGH TAPAVLGAID SVTLLPLYFE LDGTFPNHEA NPLDPANLLD LQKFVVATGA DIGLAFDGDA DRCFVVDERG EPVSPSTVTS LVAARELSRE IGATVIHNVI TSRAVPELVV ERGGTPLRSR VGHSYIKALM ADTGAIFGGE HSAHYYFRDF WGADSGMLAA LYVLAALGEQ SRPLSELTAD YQRYASSGEI NFTVADAPAC VEGVLKSFGS RIVSIDHLDG VTVDLGDDSW FNLRSSNTEP LLRLNVEARS AGDVDAVVQQ VSAEIAVQSP RGEAGP
NT Sequence
ATGTCTTGGC CCGCCGCGGT TATCAATCGT GTCATCAAGG CTTATGACGT CCGAGGGCTG GTAGGCGAAG AGATCACCGA AGCCCTGTGC GCCGATATCG GCGCCGCGTT CGCGCGGTTG ATGCGCGGCG AGGGGGCGCG GCAGGTGGTG ATTGGCTACG ACATGCGCGA CAGCTCGCCG GCGCTGGCCG CGGCCTTCGC CAGTGGGGTG GTCGCGCAAG GCCTCGGCGT GGTGCGCATC GGTCTGGGAT CCACCGACCA GCTGTATTTC GCCGCCGGGC AGCTCGATTG TCCGGGCGCG ATGTTCACTG CCAGCCACAA CCCGGCGGCC TACAACGGCA TCAAGCTGTG CCGGGCCGGC GCCAAGCCGG TCGGTGCGGA CACCGGGCTC AACGCCATTC GCGAGGACCT GGTCAATCCG GTGGCGATCG CCGTGGCCGA GACCGCCGGA ACAATCGCCG ACAAGGATGT GCTCGGGGAC TACGGGGTGT TTCTGCGCTC GCTGGTCAAC ACCGCGGATC TGCGACCGCT GCGCGTGGCC GTGGACGCCG GTAACGGGAT GGCCGGGCAC ACCGCGCCGG CCGTGCTCGG TGCGATCGAT TCGGTCACGC TGTTGCCGTT GTATTTTGAG CTCGACGGCA CCTTCCCCAA CCATGAAGCC AACCCGCTGG ACCCGGCGAA TCTGCTGGAC CTGCAGAAGT TCGTGGTGGC AACCGGTGCC GACATCGGGC TGGCCTTCGA CGGGGATGCC GACCGCTGCT TCGTGGTGGA CGAGCGTGGG GAGCCGGTCT CTCCATCGAC GGTGACCAGC CTGGTGGCGG CGCGCGAGCT CAGTCGGGAG ATCGGCGCCA CCGTGATCCA CAACGTGATC ACCTCACGCG CGGTGCCCGA GCTGGTCGTC GAGCGCGGCG GCACGCCGCT GCGTTCGCGG GTCGGCCACT CCTACATCAA GGCCCTGATG GCCGACACCG GCGCGATATT CGGCGGGGAG CATTCGGCGC ACTACTACTT CCGCGACTTC TGGGGCGCCG ACTCGGGAAT GCTGGCCGCG CTGTACGTGC TGGCGGCCCT CGGTGAGCAG AGCCGACCAT TGTCGGAGTT GACCGCCGAC TATCAGCGCT ATGCATCCTC GGGCGAGATC AATTTCACCG TGGCTGACGC GCCGGCGTGT GTCGAGGGCG TCTTGAAGTC ATTTGGTAGC AGGATTGTGT CCATCGACCA CCTGGATGGG GTGACGGTGG ATTTGGGTGA CGACAGCTGG TTCAACCTGC GCAGCTCCAA CACCGAGCCG CTGCTGCGGC TCAATGTGGA AGCCCGCAGC GCCGGGGATG TCGACGCGGT GGTCCAACAG GTCAGCGCGG AAATCGCTGT CCAGTCACCC CGGGGAGAGG CGGGACCG
Details for MyulA.01370.a.A1.GE29889
HARVESTED ON: 7/20/2010
SEQUENCED ON: 8/2/2010
EXPECTED MW: 50kDa
OBSERVED MW: 48kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 95
Validated AA Sequence
MSWPAAVINR VIKAYDVRGL VGEEITEALC ADIGAAFARL MRGEGARQVV IGYDMRDSSP ALAAAFASGV VAQGLGVVRI GLGSTDQLYF AAGQLDCPGA MFTASHNPAA YNGIKLCRAG AKPVGADTGL NAIREDLVNP VAIAVAETAG TIADKDVLGD YGVFLRSLVN TADLRPLRVA VDAGNGMAGH TAPAVLGAID SVTLLPLYFE LDGTFPNHEA NPLDPANLLD LQKFVVATGA DIGLAFDGDA DRCFVVDERG EPVSPSTVTS LVAARELSRE IGATVIHNVI TSRAVPELVV ERGGTPLRSR VGHSYIKALM ADTGAIFGGE HSAHYYFRDF WGADSGMLAA LYVLAALGEQ SRPLSELTAD YQRYASSGEI NFTVADAPAC VEGVLKSFGS RIVSIDHLDG VTVDLGDDSW FNLRSSNTEP LLRLNVEARS AGDVDAVVQQ VSAEIAVQSP RGEAG
Validated NT Sequence
tatcccgcct ctccccgggg tgactggaca gcgatttccg cgctgacctg ttggaccacc gcgtcgacat ccccggcgct gcgggcttcc acattgagcc gcagcagcgg ctcggtgttg gagctgcgca ggttgaacca gctgtcgtca cccaaatcca ccgtcacccc atccaggtgg tcgatggaca caatcctgct accaaatgac ttcaagacgc cctcgacaca cgccggcgcg tcagccacgg tgaaattgat ctcgcccgag gatgcatagc gctgatagtc ggcggtcaac tccgacaatg gtcggctctg ctcaccgagg gccgccagca cgtacagcgc ggccagcatt cccgagtcgg cgccccagaa gtcgcggaag tagtagtgcg ccgaatgctc cccgccgaat atcgcgccgg tgtcggccat cagggccttg atgtaggagt ggccgacccg cgaacgcagc ggcgtgccgc cgcgctcgac gaccagctcg ggcaccgcgc gtgaggtgat cacgttgtgg atcacggtgg cgccgatctc ccgactgagc tcgcgcgccg ccaccaggct ggtcaccgtc gatggagaga ccggctcccc acgctcgtcc accacgaagc agcggtcggc atccccgtcg aaggccagcc cgatgtcggc accggttgcc accacgaact tctgcaggtc cagcagattc gccgggtcca gcgggttggc ttcatggttg gggaaggtgc cgtcgagctc aaaatacaac ggcaacagcg tgaccgaatc gatcgcaccg agcacggccg gcgcggtgtg cccggccatc ccgttaccgg cgtccacggc cacgcgcagc ggtcgcagat ccgcggtgtt gaccagcgag cgcagaaaca ccccgtagtc cccgagcaca tccttgtcgg cgattgttcc ggcggtctcg gccacggcga tcgccaccgg attgaccagg tcctcgcgaa tggcgttgag cccggtgtcc gcaccgaccg gcttggcgcc ggcccggcac agcttgatgc cgttgtaggc cgccgggttg tggctggcag tgaacatcgc gcccggacaa tcgagctgcc cggcggcgaa atacagctgg tcggtggatc ccagaccgat gcgcaccacg ccgaggcctt gcgcgaccac cccactggcg aaggccgcgg ccagcgccgg cgagctgtcg cgcatgtcgt agccaatcac cacctgccgc gccccctcgc cgcgcatcaa ccgcgcgaac gcggcgccga tatcggcgca cagggcttcg gtgatctctt cgcctaccag ccctcggacg tcataagcct tgatgacacg attgataacc gcggcgggcc aagacatcga accaggaccc tgggtctgag cttccaggg
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSWPAAVIN RVIKAYDVRG LVGEEITEAL CADIGAAFAR LMRGEGARQV VIGYDMRDSS PALAAAFASG VVAQGLGVVR IGLGSTDQLY FAAGQLDCPG AMFTASHNPA AYNGIKLCRA GAKPVGADTG LNAIREDLVN PVAIAVAETA GTIADKDVLG DYGVFLRSLV NTADLRPLRV AVDAGNGMAG HTAPAVLGAI DSVTLLPLYF ELDGTFPNHE ANPLDPANLL DLQKFVVATG ADIGLAFDGD ADRCFVVDER GEPVSPSTVT SLVAARELSR EIGATVIHNV ITSRAVPELV VERGGTPLRS RVGHSYIKAL MADTGAIFGG EHSAHYYFRD FWGADSGMLA ALYVLAALGE QSRPLSELTA DYQRYASSGE INFTVADAPA CVEGVLKSFG SRIVSIDHLD GVTVDLGDDS WFNLRSSNTE PLLRLNVEAR SAGDVDAVVQ QVSAEIAVQS PRGEAG
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gtcttggccc gccgcggtta tcaatcgtgt catcaaggct tatgacgtcc gagggctggt aggcgaagag atcaccgaag ccctgtgcgc cgatatcggc gccgcgttcg cgcggttgat gcgcggcgag ggggcgcggc aggtggtgat tggctacgac atgcgcgaca gctcgccggc gctggccgcg gccttcgcca gtggggtggt cgcgcaaggc ctcggcgtgg tgcgcatcgg tctgggatcc accgaccagc tgtatttcgc cgccgggcag ctcgattgtc cgggcgcgat gttcactgcc agccacaacc cggcggccta caacggcatc aagctgtgcc gggccggcgc caagccggtc ggtgcggaca ccgggctcaa cgccattcgc gaggacctgg tcaatccggt ggcgatcgcc gtggccgaga ccgccggaac aatcgccgac aaggatgtgc tcggggacta cggggtgttt ctgcgctcgc tggtcaacac cgcggatctg cgaccgctgc gcgtggccgt ggacgccggt aacgggatgg ccgggcacac cgcgccggcc gtgctcggtg cgatcgattc ggtcacgctg ttgccgttgt attttgagct cgacggcacc ttccccaacc atgaagccaa cccgctggac ccggcgaatc tgctggacct gcagaagttc gtggtggcaa ccggtgccga catcgggctg gccttcgacg gggatgccga ccgctgcttc gtggtggacg agcgtgggga gccggtctct ccatcgacgg tgaccagcct ggtggcggcg cgcgagctca gtcgggagat cggcgccacc gtgatccaca acgtgatcac ctcacgcgcg gtgcccgagc tggtcgtcga gcgcggcggc acgccgctgc gttcgcgggt cggccactcc tacatcaagg ccctgatggc cgacaccggc gcgatattcg gcggggagca ttcggcgcac tactacttcc gcgacttctg gggcgccgac tcgggaatgc tggccgcgct gtacgtgctg gcggccctcg gtgagcagag ccgaccattg tcggagttga ccgccgacta tcagcgctat gcatcctcgg gcgagatcaa tttcaccgtg gctgacgcgc cggcgtgtgt cgagggcgtc ttgaagtcat ttggtagcag gattgtgtcc atcgaccacc tggatggggt gacggtggat ttgggtgacg acagctggtt caacctgcgc agctccaaca ccgagccgct gctgcggctc aatgtggaag cccgcagcgc cggggatgtc gacgcggtgg tccaacaggt cagcgcggaa atcgctgtcc agtcaccccg gggagaggcg ggaaaacagc acgaacaagt tctgcagcca agcttctcga ggatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt aaagcttatc gatgataagc tgtcaaacat gagaa
Details for MyulA.01370.a.A1.PS00791
PURIFICATION DATe: 10/7/2010
CONCENTRATION: 27.45mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Moderate Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 4
VIAL VOLUME: data unavailable
PERCENT IDENTITY: 100
PERCENT COVERAGE: 95
Protocol Notes
notes unavailable
Validated AA Sequence
MSWPAAVINR VIKAYDVRGL VGEEITEALC ADIGAAFARL MRGEGARQVV IGYDMRDSSP ALAAAFASGV VAQGLGVVRI GLGSTDQLYF AAGQLDCPGA MFTASHNPAA YNGIKLCRAG AKPVGADTGL NAIREDLVNP VAIAVAETAG TIADKDVLGD YGVFLRSLVN TADLRPLRVA VDAGNGMAGH TAPAVLGAID SVTLLPLYFE LDGTFPNHEA NPLDPANLLD LQKFVVATGA DIGLAFDGDA DRCFVVDERG EPVSPSTVTS LVAARELSRE IGATVIHNVI TSRAVPELVV ERGGTPLRSR VGHSYIKALM ADTGAIFGGE HSAHYYFRDF WGADSGMLAA LYVLAALGEQ SRPLSELTAD YQRYASSGEI NFTVADAPAC VEGVLKSFGS RIVSIDHLDG VTVDLGDDSW FNLRSSNTEP LLRLNVEARS AGDVDAVVQQ VSAEIAVQSP RGEAG
Validated NT Sequence
tatcccgcct ctccccgggg tgactggaca gcgatttccg cgctgacctg ttggaccacc gcgtcgacat ccccggcgct gcgggcttcc acattgagcc gcagcagcgg ctcggtgttg gagctgcgca ggttgaacca gctgtcgtca cccaaatcca ccgtcacccc atccaggtgg tcgatggaca caatcctgct accaaatgac ttcaagacgc cctcgacaca cgccggcgcg tcagccacgg tgaaattgat ctcgcccgag gatgcatagc gctgatagtc ggcggtcaac tccgacaatg gtcggctctg ctcaccgagg gccgccagca cgtacagcgc ggccagcatt cccgagtcgg cgccccagaa gtcgcggaag tagtagtgcg ccgaatgctc cccgccgaat atcgcgccgg tgtcggccat cagggccttg atgtaggagt ggccgacccg cgaacgcagc ggcgtgccgc cgcgctcgac gaccagctcg ggcaccgcgc gtgaggtgat cacgttgtgg atcacggtgg cgccgatctc ccgactgagc tcgcgcgccg ccaccaggct ggtcaccgtc gatggagaga ccggctcccc acgctcgtcc accacgaagc agcggtcggc atccccgtcg aaggccagcc cgatgtcggc accggttgcc accacgaact tctgcaggtc cagcagattc gccgggtcca gcgggttggc ttcatggttg gggaaggtgc cgtcgagctc aaaatacaac ggcaacagcg tgaccgaatc gatcgcaccg agcacggccg gcgcggtgtg cccggccatc ccgttaccgg cgtccacggc cacgcgcagc ggtcgcagat ccgcggtgtt gaccagcgag cgcagaaaca ccccgtagtc cccgagcaca tccttgtcgg cgattgttcc ggcggtctcg gccacggcga tcgccaccgg attgaccagg tcctcgcgaa tggcgttgag cccggtgtcc gcaccgaccg gcttggcgcc ggcccggcac agcttgatgc cgttgtaggc cgccgggttg tggctggcag tgaacatcgc gcccggacaa tcgagctgcc cggcggcgaa atacagctgg tcggtggatc ccagaccgat gcgcaccacg ccgaggcctt gcgcgaccac cccactggcg aaggccgcgg ccagcgccgg cgagctgtcg cgcatgtcgt agccaatcac cacctgccgc gccccctcgc cgcgcatcaa ccgcgcgaac gcggcgccga tatcggcgca cagggcttcg gtgatctctt cgcctaccag ccctcggacg tcataagcct tgatgacacg attgataacc gcggcgggcc aagacatcga accaggaccc tgggtctgag cttccaggg
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMSWPAAVIN RVIKAYDVRG LVGEEITEAL CADIGAAFAR LMRGEGARQV VIGYDMRDSS PALAAAFASG VVAQGLGVVR IGLGSTDQLY FAAGQLDCPG AMFTASHNPA AYNGIKLCRA GAKPVGADTG LNAIREDLVN PVAIAVAETA GTIADKDVLG DYGVFLRSLV NTADLRPLRV AVDAGNGMAG HTAPAVLGAI DSVTLLPLYF ELDGTFPNHE ANPLDPANLL DLQKFVVATG ADIGLAFDGD ADRCFVVDER GEPVSPSTVT SLVAARELSR EIGATVIHNV ITSRAVPELV VERGGTPLRS RVGHSYIKAL MADTGAIFGG EHSAHYYFRD FWGADSGMLA ALYVLAALGE QSRPLSELTA DYQRYASSGE INFTVADAPA CVEGVLKSFG SRIVSIDHLD GVTVDLGDDS WFNLRSSNTE PLLRLNVEAR SAGDVDAVVQ QVSAEIAVQS PRGEAG
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GTCTTGGCCC GCCGCGGTTA TCAATCGTGT CATCAAGGCT TATGACGTCC GAGGGCTGGT AGGCGAAGAG ATCACCGAAG CCCTGTGCGC CGATATCGGC GCCGCGTTCG CGCGGTTGAT GCGCGGCGAG GGGGCGCGGC AGGTGGTGAT TGGCTACGAC ATGCGCGACA GCTCGCCGGC GCTGGCCGCG GCCTTCGCCA GTGGGGTGGT CGCGCAAGGC CTCGGCGTGG TGCGCATCGG TCTGGGATCC ACCGACCAGC TGTATTTCGC CGCCGGGCAG CTCGATTGTC CGGGCGCGAT GTTCACTGCC AGCCACAACC CGGCGGCCTA CAACGGCATC AAGCTGTGCC GGGCCGGCGC CAAGCCGGTC GGTGCGGACA CCGGGCTCAA CGCCATTCGC GAGGACCTGG TCAATCCGGT GGCGATCGCC GTGGCCGAGA CCGCCGGAAC AATCGCCGAC AAGGATGTGC TCGGGGACTA CGGGGTGTTT CTGCGCTCGC TGGTCAACAC CGCGGATCTG CGACCGCTGC GCGTGGCCGT GGACGCCGGT AACGGGATGG CCGGGCACAC CGCGCCGGCC GTGCTCGGTG CGATCGATTC GGTCACGCTG TTGCCGTTGT ATTTTGAGCT CGACGGCACC TTCCCCAACC ATGAAGCCAA CCCGCTGGAC CCGGCGAATC TGCTGGACCT GCAGAAGTTC GTGGTGGCAA CCGGTGCCGA CATCGGGCTG GCCTTCGACG GGGATGCCGA CCGCTGCTTC GTGGTGGACG AGCGTGGGGA GCCGGTCTCT CCATCGACGG TGACCAGCCT GGTGGCGGCG CGCGAGCTCA GTCGGGAGAT CGGCGCCACC GTGATCCACA ACGTGATCAC CTCACGCGCG GTGCCCGAGC TGGTCGTCGA GCGCGGCGGC ACGCCGCTGC GTTCGCGGGT CGGCCACTCC TACATCAAGG CCCTGATGGC CGACACCGGC GCGATATTCG GCGGGGAGCA TTCGGCGCAC TACTACTTCC GCGACTTCTG GGGCGCCGAC TCGGGAATGC TGGCCGCGCT GTACGTGCTG GCGGCCCTCG GTGAGCAGAG CCGACCATTG TCGGAGTTGA CCGCCGACTA TCAGCGCTAT GCATCCTCGG GCGAGATCAA TTTCACCGTG GCTGACGCGC CGGCGTGTGT CGAGGGCGTC TTGAAGTCAT TTGGTAGCAG GATTGTGTCC ATCGACCACC TGGATGGGGT GACGGTGGAT TTGGGTGACG ACAGCTGGTT CAACCTGCGC AGCTCCAACA CCGAGCCGCT GCTGCGGCTC AATGTGGAAG CCCGCAGCGC CGGGGATGTC GACGCGGTGG TCCAACAGGT CAGCGCGGAA ATCGCTGTCC AGTCACCCCG GGGAGAGGCG GGAAAACAGC ACGAACAAGT TCTGCAGCCA AGCTTCTCGA GGATCCGGCT GCTAACAAAG CCCGAAAGGA AGCTGAGTTG GCTGCTGCCA CCGCTGAGCA ATAACTAGCA TAACCCCTTG GGGCCTCTAA ACGGGTCTTG AGGGGTTTTT TGCTGAAAGG AGGAACTATA TCCGGATATC CACAGGACGG GTGTGGTCGC CATGATCGCG TAGTCGATAG TGGCTCCAAG TAGCGAAGCG AGCAGGACTG GGCGGCGGCC AAAGCGGTCG GACAGTGCTC CGAGAACGGG TGCGCATAGA AATTGCATCA ACGCATATAG CGCTAGCAGC ACGCCATAGT GACTGGCGAT GCTGTCGGAA TGGACGATAT CCCGCAAGAG GCCCGGCAGT ACCGGCATAA CCAAGCCTAT GCCTACAGCA TCCAGGGTGA CGGTGCCGAG GATGACGATG AGCGCATTGT TAGATTTCAT ACACGGTGCC TGACTGCGTT AGCAATTTAA CTGTGATAAA CTACCGCATT AAAGCTTATC GATGATAAGC TGTCAAACAT GAGAA