MyulA.01326.c

Dehydrogenase

CENTER ID: MyulA.01326.c
ORGANISM: Mycobacterium ulcerans Agy99
ASSOCIATED DISEASE: Buruli, Bairnsdale ulcer
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

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Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyulA.01326.c.A1.PS00832 Full length( MyulA.01326.c ) 1 293

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|362242.7.peg.2784
RefSeq: YP_906194.1
UniProt: A0PQV4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTSVQDKVVF ITGGARGIGA EVARRLHNMG AKLVLTDLSE AELATIAAEL GRDDRVLTVV ADVRDLPAME AAADQAVEKF GGIDIVMANA GIASYGSVLQ VDPEAFKRVL DVNVMGVFHT VRATLPAVIE RRGYVLIVSS LAAYAASPGL APYNASKAAV EIFANALRLE VADRGVSVGS AHMSWIDTAL VRDTKGDLPA FAELLSRLPW PLNKTTSVNK CAAAFIKGIE GRKTRIYCPD WVGLFRWLKP ALSTPLGELP VRKATAELLP KMDAEMAALG RATSAYNQGL EKP
NT Sequence
ATGACATCGG TGCAGGACAA AGTCGTCTTC ATCACGGGCG GTGCTCGCGG CATTGGAGCA GAGGTCGCCC GTCGCCTCCA CAACATGGGC GCCAAACTGG TGCTGACGGA TCTGAGCGAG GCCGAGCTGG CGACGATTGC GGCCGAACTC GGTAGAGACG ACCGAGTGCT CACCGTGGTT GCCGACGTAC GCGATCTGCC CGCCATGGAG GCGGCCGCCG ATCAGGCCGT CGAGAAATTC GGCGGCATCG ACATCGTGAT GGCCAACGCC GGCATCGCCA GCTACGGTTC GGTGCTGCAG GTGGATCCGG AGGCGTTCAA GCGGGTGCTC GACGTCAACG TTATGGGTGT CTTTCACACC GTGCGTGCCA CCTTGCCGGC GGTCATCGAA CGCCGCGGCT ACGTGCTGAT CGTCTCTTCG CTGGCCGCCT ACGCGGCATC TCCCGGGCTG GCGCCTTACA ACGCGTCGAA GGCCGCGGTC GAGATCTTCG CCAACGCGCT TCGACTGGAA GTCGCAGACC GTGGCGTCAG CGTCGGGTCG GCGCACATGT CGTGGATCGA CACCGCTTTG GTTCGTGACA CCAAGGGCGA CCTGCCCGCG TTTGCTGAGC TGCTGTCCAG GCTGCCCTGG CCATTGAACA AGACCACCTC GGTGAACAAG TGCGCGGCCG CCTTCATCAA GGGCATCGAA GGTCGCAAGA CCCGTATCTA CTGCCCGGAT TGGGTGGGAC TGTTCCGCTG GCTCAAGCCA GCGCTCTCGA CGCCGCTGGG TGAACTGCCC GTCCGTAAGG CCACAGCCGA GCTACTGCCC AAGATGGACG CTGAGATGGC GGCGCTCGGG CGCGCCACCA GTGCCTACAA CCAGGGCTTG GAAAAGCCG
Details for MyulA.01326.c.A1.PS00832
PURIFICATION DATe: 10/26/2010
CONCENTRATION: 32.5mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 5
VIAL VOLUME: 200µl
PERCENT IDENTITY: 100
PERCENT COVERAGE: 70
Protocol Notes
notes unavailable
Validated AA Sequence
MEAAADQAVE KFGGIDIVMA NAGIASYGSV LQVDPEAFKR VLDVNVMGVF HTVRATLPAV IERRGYVLIV SSLAAYAASP GLAPYNASKA AVEIFANALR LEVADRGVSV GSAHMSWIDT ALVRDTKGDL PAFAELLSRL PWPLNKTTSV NKCAAAFIKG IEGRKTRIYC PDWVGLFRWL KPALSTPLGE LPVRKATAEL LPKMDAEMAA LGRATSAYNQ GL
Validated NT Sequence
ccaagccctg gttgtaggca ctggtggcgc gcccgagcgc cgccatctca gcgtccatct tgggcagtag ctcggctgtg gccttacgga cgggcagttc acccagcggc gtcgagagcg ctggcttgag ccagcggaac agtcccaccc aatccgggca gtagatacgg gtcttgcgac cttcgatgcc cttgatgaag gcggccgcgc acttgttcac cgaggtggtc ttgttcaatg gccagggcag cctggacagc agctcagcaa acgcgggcag gtcgcccttg gtgtcacgaa ccaaagcggt gtcgatccac gacatgtgcg ccgacccgac gctgacgcca cggtctgcga cttccagtcg aagcgcgttg gcgaagatct cgaccgcggc cttcgacgcg ttgtaaggcg ccagcccggg agatgccgcg taggcggcca gcgaagagac gatcagcacg tagccgcggc gttcgatgac cgccggcaag gtggcacgca cggtgtgaaa gacacccata acgttgacgt cgagcacccg cttgaacgcc tccggatcca cctgcagcac cgaaccgtag ctggcgatgc cggcgttggc catcacgatg tcgatgccgc cgaatttctc gacggcctga tcggcggccg cctccatggc gggcagatcg cgtacgtcgg caaccacggt gagcactcgg tcgtctctac cgagttcggc cgcaatcgtc gcccngctcg gcctcgctca natccgtcag caccagtttg gcgcccatgt tgtggangcg acgggcgacc tctgctccaa tgncgcgagc accgcccgtg atgaanacna ctttgtcctg caccgatgnc atcna
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTSVQDKVV FITGGARGIG AEVARRLHNM GAKLVLTDLS EAELATIAAE LGRDDRVLTV VADVRDLPAM EAAADQAVEK FGGIDIVMAN AGIASYGSVL QVDPEAFKRV LDVNVMGVFH TVRATLPAVI ERRGYVLIVS SLAAYAASPG LAPYNASKAA VEIFANALRL EVADRGVSVG SAHMSWIDTA LVRDTKGDLP AFAELLSRLP WPLNKTTSVN KCAAAFIKGI EGRKTRIYCP DWVGLFRWLK PALSTPLGEL PVRKATAELL PKMDAEMAAL GRATSAYNQG LEK
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GACATCGGTG CAGGACAAAG TCGTCTTCAT CACGGGCGGT GCTCGCGGCA TTGGAGCAGA GGTCGCCCGT CGCCTCCACA ACATGGGCGC CAAACTGGTG CTGACGGATC TGAGCGAGGC CGAGCTGGCG ACGATTGCGG CCGAACTCGG TAGAGACGAC CGAGTGCTCA CCGTGGTTGC CGACGTACGC GATCTGCCCG CCATGGAGGC GGCCGCCGAT CAGGCCGTCG AGAAATTCGG CGGCATCGAC ATCGTGATGG CCAACGCCGG CATCGCCAGC TACGGTTCGG TGCTGCAGGT GGATCCGGAG GCGTTCAAGC GGGTGCTCGA CGTCAACGTT ATGGGTGTCT TTCACACCGT GCGTGCCACC TTGCCGGCGG TCATCGAACG CCGCGGCTAC GTGCTGATCG TCTCTTCGCT GGCCGCCTAC GCGGCATCTC CCGGGCTGGC GCCTTACAAC GCGTCGAAGG CCGCGGTCGA GATCTTCGCC AACGCGCTTC GACTGGAAGT CGCAGACCGT GGCGTCAGCG TCGGGTCGGC GCACATGTCG TGGATCGACA CCGCTTTGGT TCGTGACACC AAGGGCGACC TGCCCGCGTT TGCTGAGCTG CTGTCCAGGC TGCCCTGGCC ATTGAACAAG ACCACCTCGG TGAACAAGTG CGCGGCCGCC TTCATCAAGG GCATCGAAGG TCGCAAGACC CGTATCTACT GCCCGGATTG GGTGGGACTG TTCCGCTGGC TCAAGCCAGC GCTCTCGACG CCGCTGGGTG AACTGCCCGT CCGTAAGGCC ACAGCCGAGC TACTGCCCAA GATGGACGCT GAGATGGCGG CGCTCGGGCG CGCCACCAGT GCCTACAACC AGGGCTTGGA AAAGAAACAG CACGAACAAG TTCTGCAGCC AAGCTTCTCG AGGATCCGGC TGCTAACAAA GCCCGAAAGG AAGCTGAGTT GGCTGCTGCC ACCGCTGAGC AATAACTAGC ATAACCCCTT GGGGCCTCTA AACGGGTCTT GAGGGGTTTT TTGCTGAAAG GAGGAACTAT ATCCGGATAT CCACAGGACG GGTGTGGTCG CCATGATCGC GTAGTCGATA GTGGCTCCAA GTAGCGAAGC GAGCAGGACT GGGCGGCGGC CAAAGCGGTC GGACAGTGCT CCGAGAACGG GTGCGCATAG AAATTGCATC AACGCATATA GCGCTAGCAG CACGCCATAG TGACTGGCGA TGCTGTCGGA ATGGACGATA TCCCGCAAGA GGCCCGGCAG TACCGGCATA ACCAAGCCTA TGCCTACAGC ATCCAGGGTG ACGGTGCCGA GGATGACGAT GAGCGCATTG TTAGATTTCA TACACGGTGC CTGACTGCGT TAGCAATTTA ACTGTGATAA ACTACCGCAT TAAAGCTTAT CGATGATAAG CTGTCAAACA TGAGAA