SSGCID: Target Page

MyulA.01322.a

Succinyl-CoA:3-ketoacid-coenzyme a transferase (Alpha subunit) ScoA

CENTER ID:	MyulA.01322.a
ORGANISM:	Mycobacterium ulcerans Agy99
ASSOCIATED DISEASE:	Buruli, Bairnsdale ulcer
CURRENT STATUS:	soluble
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyulA.01322.a.A1.GE29882	Full length( MyulA.01322.a )		1	248

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|362242.7.peg.4407
RefSeq:	YP_907358.1
UniProt:	A0PU68

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MDKVVATVAE AVADITDGAS LAVGGFGLCG IPEALIAALV DSGVTDLETV SNNCGVDGVG LGVLLQHKRI RRTVSSYVGE NKEFARQFLS GELEVELTPQ GTLAERLRAG GMGIPGFYTP AGVGTQIADG GLPWRYDASG AVAVVSPPKE TREFDGATYV LERGIRTDFA LVHAWKGDRH GNLLYRYSAA NFNPECAPAG RITIAEVEHL VEPGEIGPAT VHTPGVFVQR VVHVPNPIKR IEKETVRP

NT Sequence

ATGGACAAGG TAGTGGCCAC CGTCGCGGAG GCGGTGGCAG ACATAACCGA TGGGGCGTCG CTGGCGGTCG GGGGATTCGG CCTGTGCGGC ATTCCGGAAG CGTTGATCGC GGCGCTGGTA GATAGTGGTG TCACTGACCT CGAGACGGTG TCGAACAATT GCGGTGTCGA CGGCGTCGGG CTGGGCGTGT TATTGCAGCA CAAGAGAATT CGCCGTACCG TTTCCTCCTA TGTGGGGGAG AACAAGGAGT TCGCGCGTCA GTTCCTTTCC GGCGAACTCG AGGTGGAACT GACCCCGCAG GGCACGTTGG CGGAGCGGTT GCGCGCCGGT GGTATGGGCA TCCCAGGTTT CTACACACCG GCCGGTGTTG GTACCCAGAT AGCCGACGGC GGCCTGCCCT GGCGCTACGA CGCCTCGGGC GCGGTCGCGG TGGTGTCGCC TCCCAAGGAG ACTCGTGAAT TCGATGGCGC TACTTACGTT CTCGAGCGCG GGATCCGCAC CGACTTCGCG CTGGTTCATG CGTGGAAGGG GGACCGGCAC GGCAATCTTC TGTATCGCTA CTCTGCGGCG AACTTCAACC CCGAGTGCGC TCCGGCGGGC CGGATCACGA TTGCTGAGGT CGAGCATCTT GTCGAGCCCG GCGAGATCGG CCCGGCCACA GTCCACACCC CGGGCGTGTT CGTGCAGCGG GTGGTGCATG TGCCCAATCC CATCAAGCGG ATCGAGAAAG AGACGGTGCG GCCG

Details for MyulA.01322.a.A1.GE29882

HARVESTED ON:	7/20/2010
SEQUENCED ON:	8/2/2010
EXPECTED MW:	28kDa
OBSERVED MW:	28kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Too many to count (100+)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	Low Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	97
PERCENT COVERAGE:	84

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMDKVVATVA EAVADITDGA SLAVGGFGLC GIPEALIAAL VDSGVTDLET VSNNCGVDGV GLGVLLQHKR IRRTVSSYVG ENKEFARQFL SGELEVELTP QGTLAERLRA GGMGIPGFYT PAGVGTQIAD GGLPWRYDAS GAVAVVSPPK ETREFXGATY VLERGIRTDF ALVHAXKGXR HGNLLYRYSA ANFXPXCAPA GRITIA

Validated NT Sequence

tcagcaatcg tgatccggcc cgccggagcg cactnggggt ngaagttcgc cgcagagtag cgatacagaa gattgccgtg ccgntncccc ttncacgcat gaaccagcgc gaagtcggtg cggatcccgc gctcgagaac gtaagtagcg ccatngaatt cncgagtctc cttgggaggc gacaccaccg cgaccgcgcc cgaggcgtcg tagcgccagg gcaggccgcc gtcggctatc tgggtaccaa caccggccgg tgtgtagaaa cctgggatgc ccataccacc ggcgcgcaac cgctccgcca acgtgccctg cggggtcagt tccacctcga gttcgccgga aaggaactga cgcgcgaact ccttgttctc ccccacatag gaggaaacgg tacggcgaat tctcttgtgc tgcaataaca cgcccagccc gacgccgtcg acaccgcaat tgttcgacac cgtctcgagg tcagtgacac cactatctac cagcgccgcg atcaacgctt ccggaatgcc gcacaggccg aatcccccga ccgccagcga cgccccatcg gttatgtctg ccaccgcctc cgcgacggtg gccactacct tgtccatcga accaggaccc tgggtctgag cttccagggt acccatatga tggtgatggt gatgagccat

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMDKVVATVA EAVADITDGA SLAVGGFGLC GIPEALIAAL VDSGVTDLET VSNNCGVDGV GLGVLLQHKR IRRTVSSYVG ENKEFARQFL SGELEVELTP QGTLAERLRA GGMGIPGFYT PAGVGTQIAD GGLPWRYDAS GAVAVVSPPK ETREFDGATY VLERGIRTDF ALVHAWKGDR HGNLLYRYSA ANFNPECAPA GRITIAEVEH LVEPGEIGPA TVHTPGVFVQ RVVHVPNPIK RIEKETVR

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggacaaggta gtggccaccg tcgcggaggc ggtggcagac ataaccgatg gggcgtcgct ggcggtcggg ggattcggcc tgtgcggcat tccggaagcg ttgatcgcgg cgctggtaga tagtggtgtc actgacctcg agacggtgtc gaacaattgc ggtgtcgacg gcgtcgggct gggcgtgtta ttgcagcaca agagaattcg ccgtaccgtt tcctcctatg tgggggagaa caaggagttc gcgcgtcagt tcctttccgg cgaactcgag gtggaactga ccccgcaggg cacgttggcg gagcggttgc gcgccggtgg tatgggcatc ccaggtttct acacaccggc cggtgttggt acccagatag ccgacggcgg cctgccctgg cgctacgacg cctcgggcgc ggtcgcggtg gtgtcgcctc ccaaggagac tcgtgaattc gatggcgcta cttacgttct cgagcgcggg atccgcaccg acttcgcgct ggttcatgcg tggaaggggg accggcacgg caatcttctg tatcgctact ctgcggcgaa cttcaacccc gagtgcgctc cggcgggccg gatcacgatt gctgaggtcg agcatcttgt cgagcccggc gagatcggcc cggccacagt ccacaccccg ggcgtgttcg tgcagcgggt ggtgcatgtg cccaatccca tcaagcggat cgagaaagag acggtgcgga aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a