MyulA.01263.b

Acyl-CoA dehydrogenase FadE19

CENTER ID: MyulA.01263.b
ORGANISM: Mycobacterium ulcerans Agy99
ASSOCIATED DISEASE: Buruli, Bairnsdale ulcer
CURRENT STATUS: purified
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:
IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
MyulA.01263.b.A1.GE29880 Full length( MyulA.01263.b ) 1 388
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID
DOMAIN/REGION
DESCRIPTION
INFO AA
START
AA
STOP
ORDER
MATERIAL
MyulA.01263.b.A1.PS00796 Full length( MyulA.01263.b ) 1 388

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|362242.7.peg.4401
RefSeq: YP_907355.1
UniProt: A0PU65

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MTTAISAGTL PKEYQDLRDT VADFARSVVA PVSAKHDEEH SFPYEVVAKM GEMGLFGLPF PEEYGGMGGD YFALALALEE LGKVNQSVAI TLEAGVGLGA MPIYRFGNEE QKSKWLPDLL AGRALAGFGL TEPGAGSDAG STRTTARLDG GEWVVNGSKQ FITNSGTDIT SLVTITAVTG SIADGKKEIS TIIVPSGTPG FIVEPVYNKV GWNASDTHPL SFDDARVPEE NLLGIRGKGY ANFLSILDEG RIAIAALATG VAQGCVDESV KYAKERQSFG QPIGSYQAIS FKIARMEARA HVARTAYYEA AAKMLAGKPF KKEAAIAKMI SSEAAMDNAR DAAQVHGGYG FMNEYPVARH YRDSKILEIG EGTTEVQLML IARSLGLS
NT Sequence
ATGACGACAG CGATCTCCGC AGGAACATTA CCCAAGGAAT ATCAGGATCT GCGCGACACG GTGGCCGACT TCGCGCGGTC GGTGGTTGCG CCGGTATCGG CCAAACACGA TGAGGAACAT AGCTTTCCCT ACGAAGTCGT GGCCAAGATG GGCGAGATGG GGTTGTTCGG CCTGCCGTTC CCCGAAGAAT ACGGGGGAAT GGGCGGGGAC TACTTCGCGC TAGCCCTAGC GCTCGAGGAG CTCGGCAAGG TTAACCAGTC GGTGGCGATC ACCCTGGAAG CGGGTGTGGG TCTGGGCGCC ATGCCGATCT ACCGGTTCGG CAACGAGGAA CAGAAGAGCA AGTGGTTGCC GGATCTGCTG GCCGGGCGTG CGCTGGCCGG TTTCGGGCTC ACCGAACCTG GCGCGGGCTC GGACGCCGGC AGCACCCGCA CCACCGCACG CCTCGACGGC GGCGAATGGG TCGTCAACGG CAGTAAGCAG TTCATCACCA ACTCAGGCAC CGACATCACC TCATTGGTCA CCATCACCGC CGTCACGGGG AGCATCGCCG ATGGCAAGAA GGAGATCTCG ACGATCATCG TGCCCAGCGG CACGCCGGGA TTCATCGTGG AACCGGTCTA CAACAAGGTC GGCTGGAACG CGTCGGACAC CCACCCGCTG AGCTTCGATG ACGCGCGCGT GCCGGAAGAG AATCTGCTTG GAATCCGTGG CAAGGGCTAC GCGAATTTCC TATCCATCCT CGACGAGGGC CGAATCGCGA TCGCCGCGTT GGCGACAGGT GTCGCTCAGG GCTGTGTCGA CGAAAGCGTC AAGTATGCCA AGGAGCGTCA GTCCTTCGGC CAGCCGATCG GTTCCTATCA GGCGATCAGC TTCAAGATCG CGCGCATGGA AGCACGGGCA CACGTGGCCC GCACCGCGTA CTACGAGGCT GCCGCAAAGA TGTTGGCGGG CAAGCCCTTC AAGAAGGAGG CGGCGATCGC CAAGATGATC TCCTCGGAGG CTGCGATGGA CAATGCCCGC GACGCGGCCC AGGTCCACGG CGGCTACGGC TTCATGAACG AGTACCCGGT GGCCCGTCAC TATCGCGACA GCAAGATCCT TGAGATCGGC GAGGGCACCA CCGAGGTGCA GTTGATGCTC ATCGCCCGTT CACTGGGCCT GTCG
Details for MyulA.01263.b.A1.GE29880
HARVESTED ON: 7/20/2010
SEQUENCED ON: 8/2/2010
EXPECTED MW: 43kDa
OBSERVED MW: 43kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL High Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 94
Validated AA Sequence
MTTAISAGTL PKEYQDLRDT VADFARSVVA PVSAKHDEEH SFPYEVVAKM GEMGLFGLPF PEEYGGMGGD YFALALALEE LGKVNQSVAI TLEAGVGLGA MPIYRFGNEE QKSKWLPDLL AGRALAGFGL TEPGAGSDAG STRTTARLDG GEWVVNGSKQ FITNSGTDIT SLVTITAVTG SIADGKKEIS TIIVPSGTPG FIVEPVYNKV GWNASDTHPL SFDDARVPEE NLLGIRGKGY ANFLSILDEG RIAIAALATG VAQGCVDESV KYAKERQSFG QPIGSYQAIS FKIARMEARA HVARTAYYEA AAKMLAGKPF KKEAAIAKMI SSEAAMDNAR DAAQVHGGYG FMNEYPVARH YRDSKILEIG EGTTEVQLML IARSLGL
Validated NT Sequence
ttacaggccc agtgaacggg cgatgagcat caactgcacc tcggtggtgc cctcgccgat ctcaaggatc ttgctgtcgc gatagtgacg ggccaccggg tactcgttca tgaagccgta gccgccgtgg acctgggccg cgtcgcgggc attgtccatc gcagcctccg aggagatcat cttggcgatc gccgcctcct tcttgaaggg cttgcccgcc aacatctttg cggcagcctc gtagtacgcg gtgcgggcca cgtgtgcccg tgcttccatg cgcgcgatct tgaagctgat cgcctgatag gaaccgatcg gctggccgaa ggactgacgc tccttggcat acttgacgct ttcgtcgaca cagccctgag cgacacctgt cgccaacgcg gcgatcgcga ttcggccctc gtcgaggatg gataggaaat tcgcgtagcc cttgccacgg attccaagca gattctcttc cggcacgcgc gcgtcatcga agctcagcgg gtgggtgtcc gacgcgttcc agccgacctt gttgtagacc ggttccacga tgaatcccgg cgtgccgctg ggcacgatga tcgtcgagat ctccttcttg ccatcggcga tgctccccgt gacggcggtg atggtgacca atgaggtgat gtcggtgcct gagttggtga tgaactgctt actgccgttg acgacccatt cgccgccgtc gaggcgtgcg gtggtgcggg tgctgccggc gtccgagccc gcgccaggtt cggtgagccc gaaaccggcc agcgcacgcc cggccagcag atccggcaac cacttgctct tctgttcctc gttgccgaac cggtagatcg gcatggcgcc cagacccaca cccgcttcca gggtgatcgc caccgactgg ttaaccttgc cgagctcctc gagcgctagg gctagcgcga agtagtcccc gcccattccc ccgtattctt cggggaacgg caggccgaac aaccccatct cgcccatctt ggccacgact tcgtagggaa agctatgttc ctcatcgtgt ttggccgata ccggcgcaac caccgaccgc gcgaagtcgg ccaccgtgtc gcgcagatcc tgatattcct tgggtaatgt tcctgcggag atcgctgtcg tcatcgaacc aggaccctgg gtctgagctt ccagggt
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTTAISAGT LPKEYQDLRD TVADFARSVV APVSAKHDEE HSFPYEVVAK MGEMGLFGLP FPEEYGGMGG DYFALALALE ELGKVNQSVA ITLEAGVGLG AMPIYRFGNE EQKSKWLPDL LAGRALAGFG LTEPGAGSDA GSTRTTARLD GGEWVVNGSK QFITNSGTDI TSLVTITAVT GSIADGKKEI STIIVPSGTP GFIVEPVYNK VGWNASDTHP LSFDDARVPE ENLLGIRGKG YANFLSILDE GRIAIAALAT GVAQGCVDES VKYAKERQSF GQPIGSYQAI SFKIARMEAR AHVARTAYYE AAAKMLAGKP FKKEAAIAKM ISSEAAMDNA RDAAQVHGGY GFMNEYPVAR HYRDSKILEI GEGTTEVQLM LIARSLGL
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gacgacagcg atctccgcag gaacattacc caaggaatat caggatctgc gcgacacggt ggccgacttc gcgcggtcgg tggttgcgcc ggtatcggcc aaacacgatg aggaacatag ctttccctac gaagtcgtgg ccaagatggg cgagatgggg ttgttcggcc tgccgttccc cgaagaatac gggggaatgg gcggggacta cttcgcgcta gccctagcgc tcgaggagct cggcaaggtt aaccagtcgg tggcgatcac cctggaagcg ggtgtgggtc tgggcgccat gccgatctac cggttcggca acgaggaaca gaagagcaag tggttgccgg atctgctggc cgggcgtgcg ctggccggtt tcgggctcac cgaacctggc gcgggctcgg acgccggcag cacccgcacc accgcacgcc tcgacggcgg cgaatgggtc gtcaacggca gtaagcagtt catcaccaac tcaggcaccg acatcacctc attggtcacc atcaccgccg tcacggggag catcgccgat ggcaagaagg agatctcgac gatcatcgtg cccagcggca cgccgggatt catcgtggaa ccggtctaca acaaggtcgg ctggaacgcg tcggacaccc acccgctgag cttcgatgac gcgcgcgtgc cggaagagaa tctgcttgga atccgtggca agggctacgc gaatttccta tccatcctcg acgagggccg aatcgcgatc gccgcgttgg cgacaggtgt cgctcagggc tgtgtcgacg aaagcgtcaa gtatgccaag gagcgtcagt ccttcggcca gccgatcggt tcctatcagg cgatcagctt caagatcgcg cgcatggaag cacgggcaca cgtggcccgc accgcgtact acgaggctgc cgcaaagatg ttggcgggca agcccttcaa gaaggaggcg gcgatcgcca agatgatctc ctcggaggct gcgatggaca atgcccgcga cgcggcccag gtccacggcg gctacggctt catgaacgag tacccggtgg cccgtcacta tcgcgacagc aagatccttg agatcggcga gggcaccacc gaggtgcagt tgatgctcat cgcccgttca ctgggcctga aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a
Details for MyulA.01263.b.A1.PS00796
PURIFICATION DATe: 9/20/2010
CONCENTRATION: 2.27mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 17
VIAL VOLUME: data unavailable
PERCENT IDENTITY: 100
PERCENT COVERAGE: 94
Protocol Notes
notes unavailable
Validated AA Sequence
MTTAISAGTL PKEYQDLRDT VADFARSVVA PVSAKHDEEH SFPYEVVAKM GEMGLFGLPF PEEYGGMGGD YFALALALEE LGKVNQSVAI TLEAGVGLGA MPIYRFGNEE QKSKWLPDLL AGRALAGFGL TEPGAGSDAG STRTTARLDG GEWVVNGSKQ FITNSGTDIT SLVTITAVTG SIADGKKEIS TIIVPSGTPG FIVEPVYNKV GWNASDTHPL SFDDARVPEE NLLGIRGKGY ANFLSILDEG RIAIAALATG VAQGCVDESV KYAKERQSFG QPIGSYQAIS FKIARMEARA HVARTAYYEA AAKMLAGKPF KKEAAIAKMI SSEAAMDNAR DAAQVHGGYG FMNEYPVARH YRDSKILEIG EGTTEVQLML IARSLGL
Validated NT Sequence
ttacaggccc agtgaacggg cgatgagcat caactgcacc tcggtggtgc cctcgccgat ctcaaggatc ttgctgtcgc gatagtgacg ggccaccggg tactcgttca tgaagccgta gccgccgtgg acctgggccg cgtcgcgggc attgtccatc gcagcctccg aggagatcat cttggcgatc gccgcctcct tcttgaaggg cttgcccgcc aacatctttg cggcagcctc gtagtacgcg gtgcgggcca cgtgtgcccg tgcttccatg cgcgcgatct tgaagctgat cgcctgatag gaaccgatcg gctggccgaa ggactgacgc tccttggcat acttgacgct ttcgtcgaca cagccctgag cgacacctgt cgccaacgcg gcgatcgcga ttcggccctc gtcgaggatg gataggaaat tcgcgtagcc cttgccacgg attccaagca gattctcttc cggcacgcgc gcgtcatcga agctcagcgg gtgggtgtcc gacgcgttcc agccgacctt gttgtagacc ggttccacga tgaatcccgg cgtgccgctg ggcacgatga tcgtcgagat ctccttcttg ccatcggcga tgctccccgt gacggcggtg atggtgacca atgaggtgat gtcggtgcct gagttggtga tgaactgctt actgccgttg acgacccatt cgccgccgtc gaggcgtgcg gtggtgcggg tgctgccggc gtccgagccc gcgccaggtt cggtgagccc gaaaccggcc agcgcacgcc cggccagcag atccggcaac cacttgctct tctgttcctc gttgccgaac cggtagatcg gcatggcgcc cagacccaca cccgcttcca gggtgatcgc caccgactgg ttaaccttgc cgagctcctc gagcgctagg gctagcgcga agtagtcccc gcccattccc ccgtattctt cggggaacgg caggccgaac aaccccatct cgcccatctt ggccacgact tcgtagggaa agctatgttc ctcatcgtgt ttggccgata ccggcgcaac caccgaccgc gcgaagtcgg ccaccgtgtc gcgcagatcc tgatattcct tgggtaatgt tcctgcggag atcgctgtcg tcatcgaacc aggaccctgg gtctgagctt ccagggt
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMTTAISAGT LPKEYQDLRD TVADFARSVV APVSAKHDEE HSFPYEVVAK MGEMGLFGLP FPEEYGGMGG DYFALALALE ELGKVNQSVA ITLEAGVGLG AMPIYRFGNE EQKSKWLPDL LAGRALAGFG LTEPGAGSDA GSTRTTARLD GGEWVVNGSK QFITNSGTDI TSLVTITAVT GSIADGKKEI STIIVPSGTP GFIVEPVYNK VGWNASDTHP LSFDDARVPE ENLLGIRGKG YANFLSILDE GRIAIAALAT GVAQGCVDES VKYAKERQSF GQPIGSYQAI SFKIARMEAR AHVARTAYYE AAAKMLAGKP FKKEAAIAKM ISSEAAMDNA RDAAQVHGGY GFMNEYPVAR HYRDSKILEI GEGTTEVQLM LIARSLGL
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GACGACAGCG ATCTCCGCAG GAACATTACC CAAGGAATAT CAGGATCTGC GCGACACGGT GGCCGACTTC GCGCGGTCGG TGGTTGCGCC GGTATCGGCC AAACACGATG AGGAACATAG CTTTCCCTAC GAAGTCGTGG CCAAGATGGG CGAGATGGGG TTGTTCGGCC TGCCGTTCCC CGAAGAATAC GGGGGAATGG GCGGGGACTA CTTCGCGCTA GCCCTAGCGC TCGAGGAGCT CGGCAAGGTT AACCAGTCGG TGGCGATCAC CCTGGAAGCG GGTGTGGGTC TGGGCGCCAT GCCGATCTAC CGGTTCGGCA ACGAGGAACA GAAGAGCAAG TGGTTGCCGG ATCTGCTGGC CGGGCGTGCG CTGGCCGGTT TCGGGCTCAC CGAACCTGGC GCGGGCTCGG ACGCCGGCAG CACCCGCACC ACCGCACGCC TCGACGGCGG CGAATGGGTC GTCAACGGCA GTAAGCAGTT CATCACCAAC TCAGGCACCG ACATCACCTC ATTGGTCACC ATCACCGCCG TCACGGGGAG CATCGCCGAT GGCAAGAAGG AGATCTCGAC GATCATCGTG CCCAGCGGCA CGCCGGGATT CATCGTGGAA CCGGTCTACA ACAAGGTCGG CTGGAACGCG TCGGACACCC ACCCGCTGAG CTTCGATGAC GCGCGCGTGC CGGAAGAGAA TCTGCTTGGA ATCCGTGGCA AGGGCTACGC GAATTTCCTA TCCATCCTCG ACGAGGGCCG AATCGCGATC GCCGCGTTGG CGACAGGTGT CGCTCAGGGC TGTGTCGACG AAAGCGTCAA GTATGCCAAG GAGCGTCAGT CCTTCGGCCA GCCGATCGGT TCCTATCAGG CGATCAGCTT CAAGATCGCG CGCATGGAAG CACGGGCACA CGTGGCCCGC ACCGCGTACT ACGAGGCTGC CGCAAAGATG TTGGCGGGCA AGCCCTTCAA GAAGGAGGCG GCGATCGCCA AGATGATCTC CTCGGAGGCT GCGATGGACA ATGCCCGCGA CGCGGCCCAG GTCCACGGCG GCTACGGCTT CATGAACGAG TACCCGGTGG CCCGTCACTA TCGCGACAGC AAGATCCTTG AGATCGGCGA GGGCACCACC GAGGTGCAGT TGATGCTCAT CGCCCGTTCA CTGGGCCTGA AACAGCACGA ACAAGTTCTG CAGCCAAGCT TCTCGAGGAT CCGGCTGCTA ACAAAGCCCG AAAGGAAGCT GAGTTGGCTG CTGCCACCGC TGAGCAATAA CTAGCATAAC CCCTTGGGGC CTCTAAACGG GTCTTGAGGG GTTTTTTGCT GAAAGGAGGA ACTATATCCG GATATCCACA GGACGGGTGT GGTCGCCATG ATCGCGTAGT CGATAGTGGC TCCAAGTAGC GAAGCGAGCA GGACTGGGCG GCGGCCAAAG CGGTCGGACA GTGCTCCGAG AACGGGTGCG CATAGAAATT GCATCAACGC ATATAGCGCT AGCAGCACGC CATAGTGACT GGCGATGCTG TCGGAATGGA CGATATCCCG CAAGAGGCCC GGCAGTACCG GCATAACCAA GCCTATGCCT ACAGCATCCA GGGTGACGGT GCCGAGGATG ACGATGAGCG CATTGTTAGA TTTCATACAC GGTGCCTGAC TGCGTTAGCA ATTTAACTGT GATAAACTAC CGCATTAAAG CTTATCGATG ATAAGCTGTC AAACATGAGA A