MyulA.01189.a

Quinone oxidoreductase

CENTER ID: MyulA.01189.a
ORGANISM: Mycobacterium ulcerans Agy99
ASSOCIATED DISEASE: Buruli, Bairnsdale ulcer
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyulA.01189.a.A1.GE29869 Full length( MyulA.01189.a ) 1 322
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|362242.7.peg.5496
RefSeq: YP_908149.1
UniProt: A0PWF9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MKACVVQELS GPSGLVYTEV DDVSDGGGNV VIDVRAAGVC LPDLLLTKGE YQLKLPPPFV PGLETAGVVR SAPARSGFRV GERVSAFGVL GGYAEQVAVP VSNVVRSPAE LDDAEAVSLL VNYNTMYFAL ARRAAMRPGE TVLVLGGAGG VGTAAIQIAK AMGAGQVIAV VHREGAIDYV ASVGADVVLP LTEGWVEEVH KHTHGRGVDI VVDPIGGPVF DDAIHALAVD GKLLAIGFAA GSIPTLAVNR LLRRNVGVLG VAWGEYLNRV PGSVYLFSWG LNHLVSLGLR PPPPQRFPLS EAQAALQSLA GGAVLGKIVL EP
NT Sequence
ATGAAAGCTT GTGTCGTACA AGAGCTTTCT GGTCCGTCCG GCCTGGTCTA CACCGAGGTA GACGACGTCT CAGATGGCGG CGGCAACGTC GTCATCGACG TTCGGGCGGC CGGGGTGTGC TTGCCGGATC TGCTGCTGAC CAAGGGCGAA TACCAACTGA AGCTGCCGCC GCCGTTCGTG CCGGGGCTGG AAACCGCGGG TGTCGTGCGG TCGGCGCCCG CGAGGTCGGG CTTTCGGGTA GGAGAACGGG TTTCGGCGTT CGGCGTACTC GGTGGCTACG CCGAACAAGT GGCGGTTCCG GTTTCCAACG TGGTGCGCAG CCCGGCCGAA CTCGATGACG CCGAAGCGGT GTCATTGCTG GTCAACTACA ACACCATGTA CTTCGCGCTG GCTCGCCGGG CCGCGATGCG GCCGGGTGAG ACGGTGTTGG TGCTCGGTGG TGCCGGCGGA GTTGGCACGG CGGCCATCCA GATCGCCAAG GCGATGGGCG CCGGCCAGGT GATAGCCGTG GTGCACCGGG AGGGTGCGAT CGACTATGTC GCCTCGGTGG GAGCCGATGT GGTGCTGCCC TTGACCGAGG GCTGGGTCGA GGAGGTGCAC AAGCACACGC ACGGGCGGGG CGTGGATATC GTGGTCGACC CGATTGGTGG CCCGGTCTTC GATGATGCGA TTCACGCGCT GGCGGTCGAC GGCAAGCTCC TGGCGATTGG CTTCGCCGCG GGCAGCATCC CGACGCTCGC GGTCAATCGC TTGCTGCGGC GCAACGTCGG CGTGCTGGGT GTCGCGTGGG GCGAATACCT CAACCGGGTG CCCGGTTCGG TGTACTTGTT CTCCTGGGGG CTCAATCACC TGGTTTCCCT GGGGCTGAGA CCGCCTCCGC CGCAGCGTTT TCCGCTCAGC GAGGCGCAAG CCGCGCTGCA GAGTCTGGCC GGCGGCGCCG TGCTGGGCAA GATCGTTCTG GAGCCG
Details for MyulA.01189.a.A1.GE29869
HARVESTED ON: 7/20/2010
SEQUENCED ON: 8/2/2010
EXPECTED MW: 35kDa
OBSERVED MW: 36kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 84
PERCENT COVERAGE: 104
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMKACVVQEL SGPSGLVYTE VDDVSDGGGN VVIDVRAAGV CLPDLLLTKG EYQLKLPPPF VPGLETAGVV RSAPARSGFR VGERVSAFGV LGGYAEQVAV PVSNVVRSPA ELDDAEAVSL LVNYNTMYFA LARRAAMRPG ETVLVLGGAG GVGTAAIQIA KAMGAGQVIA VVHREGAIDY VASVGADVVL PLTEGWVEEV HKHTHGRGVX IVVDPIGGPV FDDAIHALXV DGKLLAIGFA AGSIPTLAXN RLLRRNVGVL GVAWGEXLNX XXXXCXXXPG GSXXGXPGAX TXXAXFXXXX XXXXCXXXXX XAXIXXXXXX XXXXXXXXXX XXXXXX
Validated NT Sequence
cnagaannnn tttnnttaac tttaagaagg agatatacca tggctcatca ccatcaccat catatgggta ccctggaagc tcagacccag ggtcctggtt cgatgaaagc ttgtgtcgta caagagcttt ctggtccgtc cggcctggtc tacaccgagg tagacgacgt ctcagatggc ggcggcaacg tcgtcatcga cgttcgggcg gccggggtgt gcttgccgga tctgctgctg accaagggcg aataccaact gaagctgccg ccgccgttcg tgccggggct ggaaaccgcg ggtgtcgtgc ggtcggcgcc cgcgaggtcg ggctttcggg taggagaacg ggtttcggcg ttcggcgtac tcggtggcta cgccgaacaa gtggcggttc cggtttccaa cgtggtgcgc agcccggccg aactcgatga cgccgaagcg gtgtcattgc tggtcaacta caacaccatg tacttcgcgc tggctcgccg ggccgcgatg cggccgggtg agacggtgtt ggtgctcggt ggtgccggcg gagttggcac ggcggccatc cagatcgcca aggcgatggg cgccggccag gtgatagccg tggtgcaccg ggagggtgcg atcgactatg tcgcctcggt gggagccgat gtggtgctgc ccttgaccga gggctgggtc gaggaggtgc acaagcacac gcacgggcgg ggcgtgnata tcgtggtcga cccgattggt ggcccggtct tcgatgatgc gattcacgcg ctgnnggtcg acggcaagct cctggcgatt ggcttcgccg cgggcagcat cccgacgctc gcggncaatc gcttgctgcg gcgcaacgtc ggcgtgctgg gtgtcgcgtg gggcgaanac ctcaacnngg ngncnnnnnn gtgtantngt nntcctgggg gctcancnnc nggntnncct ggggctgana cgnncnccgc ancgtttnnn ntcnnnnagn ngnagcnnnn ntgcnnannc nnnnngnnnc nngctnnnat cnnnnnnntn anaannnnnn annnnnngca nnnnanntnt cnnnnnnnnn nnnnnnnang anntnnnnnt
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMKACVVQEL SGPSGLVYTE VDDVSDGGGN VVIDVRAAGV CLPDLLLTKG EYQLKLPPPF VPGLETAGVV RSAPARSGFR VGERVSAFGV LGGYAEQVAV PVSNVVRSPA ELDDAEAVSL LVNYNTMYFA LARRAAMRPG ETVLVLGGAG GVGTAAIQIA KAMGAGQVIA VVHREGAIDY VASVGADVVL PLTEGWVEEV HKHTHGRGVD IVVDPIGGPV FDDAIHALAV DGKLLAIGFA AGSIPTLAVN RLLRRNVGVL GVAWGEYLNR VPGSVYLFSW GLNHLVSLGL RPPPPQRFPL SEAQAALQSL AGGAVLGKIV LE
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gaaagcttgt gtcgtacaag agctttctgg tccgtccggc ctggtctaca ccgaggtaga cgacgtctca gatggcggcg gcaacgtcgt catcgacgtt cgggcggccg gggtgtgctt gccggatctg ctgctgacca agggcgaata ccaactgaag ctgccgccgc cgttcgtgcc ggggctggaa accgcgggtg tcgtgcggtc ggcgcccgcg aggtcgggct ttcgggtagg agaacgggtt tcggcgttcg gcgtactcgg tggctacgcc gaacaagtgg cggttccggt ttccaacgtg gtgcgcagcc cggccgaact cgatgacgcc gaagcggtgt cattgctggt caactacaac accatgtact tcgcgctggc tcgccgggcc gcgatgcggc cgggtgagac ggtgttggtg ctcggtggtg ccggcggagt tggcacggcg gccatccaga tcgccaaggc gatgggcgcc ggccaggtga tagccgtggt gcaccgggag ggtgcgatcg actatgtcgc ctcggtggga gccgatgtgg tgctgccctt gaccgagggc tgggtcgagg aggtgcacaa gcacacgcac gggcggggcg tggatatcgt ggtcgacccg attggtggcc cggtcttcga tgatgcgatt cacgcgctgg cggtcgacgg caagctcctg gcgattggct tcgccgcggg cagcatcccg acgctcgcgg tcaatcgctt gctgcggcgc aacgtcggcg tgctgggtgt cgcgtggggc gaatacctca accgggtgcc cggttcggtg tacttgttct cctgggggct caatcacctg gtttccctgg ggctgagacc gcctccgccg cagcgttttc cgctcagcga ggcgcaagcc gcgctgcaga gtctggccgg cggcgccgtg ctgggcaaga tcgttctgga gaaacagcac gaacaagttc tgcagccaag cttctcgagg atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatatcca caggacgggt gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac gcatatagcg ctagcagcac gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact accgcattaa agcttatcga tgataagctg tcaaacatga gaa