MyulA.01147.a

Cystathionine beta-synthase Cbs

CENTER ID: MyulA.01147.a
ORGANISM: Mycobacterium ulcerans Agy99
ASSOCIATED DISEASE: Buruli, Bairnsdale ulcer
CURRENT STATUS: crystallized
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyulA.01147.a.A1.GE29857 Full length( MyulA.01147.a ) 1 464
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

Proteins

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyulA.01147.a.A1.PS01028 Full length( MyulA.01147.a ) 1 464

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|362242.7.peg.319
RefSeq: YP_904425.1
UniProt: A0PKT5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRIAQHISDL IGGTPLVRLN AVVPAGIGTV AAKVEYLNPG GSSKDRIAIK MIDAAEASGA LRPGGTIVEP TSGNTGVGLA LVAQHRGYKC VFVCPDKVSE DKRNVLRAYG AEVVVCPTAV PPEDPDSYYS VSDRLVREID GAWKPDQYAN PEGPASHYAT TGPEIWADTD GKITHFVAGI GTGGTITGAG CYLKEVSGGK VRVIGADPEG SVYSGGTGRP YLVEGVGEDF WPATYDHAVP DEIIAVSDSD SFDMTRRLAR EEAMLVGGSC GMAVVAALRV AEQAGPDALV VVLLPDGGRG YMSKIFNDAW MSSYGFLRSR LDGRTDTSTV GDVLRRKSGA LPALVHTHPS ETVRDAIGIL REYGVSQMPV VGAEPPVMAG EVAGSVSERE LLSAVFSGKA KLADAVSQHM SPPLKLIGAG ELVGVAGKAL RDWDALMVVE EGKPVGVITR YDLLGFLSEG ALRR
NT Sequence
ATGCGGATCG CCCAGCACAT CAGTGATCTC ATCGGCGGCA CACCACTGGT TCGCCTGAAC GCGGTCGTTC CGGCCGGGAT CGGCACGGTG GCGGCGAAGG TCGAATATCT CAACCCTGGC GGCAGCTCCA AGGACCGGAT CGCGATCAAG ATGATCGACG CGGCCGAGGC CAGTGGCGCG CTGCGACCCG GCGGCACCAT CGTCGAGCCG ACGTCGGGCA ATACCGGGGT GGGTTTGGCC CTGGTCGCCC AGCACCGGGG CTACAAGTGC GTATTCGTCT GCCCCGACAA GGTCAGCGAG GACAAACGAA ACGTCTTGCG GGCCTACGGC GCTGAGGTGG TGGTGTGCCC GACGGCGGTG CCGCCGGAGG ATCCGGACAG CTACTACAGC GTCTCTGACC GGCTGGTCAG AGAAATCGAC GGTGCGTGGA AGCCCGACCA GTACGCCAAC CCCGAAGGCC CGGCCAGCCA CTACGCGACC ACCGGCCCGG AGATCTGGGC CGATACCGAT GGCAAGATCA CGCATTTCGT TGCCGGCATC GGCACCGGTG GAACGATCAC CGGCGCGGGG TGCTACCTCA AGGAGGTGTC GGGCGGCAAA GTCCGCGTCA TCGGCGCCGA CCCGGAGGGG TCGGTGTATT CCGGCGGGAC CGGGCGGCCC TACCTGGTCG AGGGGGTGGG CGAGGACTTC TGGCCGGCGA CCTACGACCA CGCGGTGCCC GACGAGATCA TCGCGGTGTC GGACTCCGAC TCCTTTGACA TGACCCGGCG GCTGGCCCGA GAAGAGGCGA TGCTGGTGGG CGGATCCTGC GGGATGGCGG TGGTGGCCGC CCTGCGGGTC GCCGAACAAG CAGGGCCCGA CGCGCTGGTT GTCGTGCTGC TGCCCGACGG CGGGCGCGGT TACATGTCGA AGATCTTCAA CGACGCGTGG ATGTCGTCGT ACGGCTTCTT GCGCAGCCGC CTCGACGGCA GGACCGATAC GTCCACGGTT GGTGATGTGT TGCGGCGCAA GTCCGGTGCG TTGCCGGCTC TGGTCCACAC CCACCCCTCC GAGACGGTGC GCGATGCCAT CGGAATCCTG CGGGAATATG GCGTGTCGCA GATGCCGGTG GTCGGCGCCG AACCGCCGGT GATGGCCGGT GAAGTTGCCG GCAGCGTTTC CGAACGCGAA CTGCTCTCGG CTGTATTCTC GGGCAAGGCC AAGCTGGCCG ACGCCGTCTC CCAGCACATG AGCCCGCCGC TGAAGCTGAT CGGCGCCGGT GAATTGGTCG GTGTGGCAGG CAAGGCATTG CGGGACTGGG ATGCCTTGAT GGTCGTCGAG GAGGGCAAGC CGGTCGGGGT CATCACCCGG TATGACTTGT TGGGATTCCT TTCCGAAGGG GCGTTGCGGC GC
Details for MyulA.01147.a.A1.GE29857
HARVESTED ON: 7/20/2010
SEQUENCED ON: 8/2/2010
EXPECTED MW: 50kDa
OBSERVED MW: 50kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Too many to count (100+)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 73
PERCENT COVERAGE: 52
Validated AA Sequence
MRIAQHISDL IGGTPLVRLN AVVPAGIGTV AAKVEYLNPG GSSKDRIAIK MIDAAEASGA LRPGGTIVEP TSGNTGVGLA LVAQHRGYKC VFVCPDKVSE DKRNVLRAYG AEVVVCPTAV PPEDPDSYYS VSDRLVREID GAWKPDQYAN PEGPXQPLRD HRPGDLGRYR WQDHAFRCRH RHRWNDHRRG VLPQGGVGRQ SPRHRRRPGG VGVFRRDRAA LPGRGGGRGL LAGDXRPRGA RRDHRGVGXR LL
Validated NT Sequence
ttaccgcaac gccccttcgg aaaggaatcc caacaagtca taccgggtga tgaccccgac cggcttgccc tcctcgacga ccatcaaggc atcccagtcc cgcaatgcct tgcctgccac accgaccaat tcaccggcgc cgatcagctt cagcggcggg ctcatgtgct gggagacggc gtcggccagc ttggccttgc ccgagaatac agccgagagc agttcgcgtt cggaaacgct gccggcaact tcaccggcca tcaccggcgg ttcggcgccg accaccggca tctgcgacac gccatattcc cgcaggattc cgatggcatc gcgcaccgtc tcggaggggt gggtgtggac cagagccggc aacgcaccgg acttgcgccg caacacatca ccaaccgtgg acgtatcggt cctgccgtcg aggcggctgc gcaagaagcc gtacgacgac atccacgcgt cgttgaagat cttcgacatg taaccgcgcc cgccgtcggg cagcagcacg acaaccagcg cgtcgggccc tgcttgttcg gcgacccgca gggcggccac caccgccatc ccgcaggatc cgcccaccag catcgcctct tctcgggcca gccgccgggt catgtcaaag gagtcggant ccgacaccgc gatgatctcg tcgggcaccg cgtggtcgta ngtcgccggc cagaagtcct cgcccacccc ctcgaccagg tagggccgcc cggtcccgcc ggaatacacc gacccctccg ggtcggcgcc gatgacgcgg actttgccgc ccgacacctc cttgaggtag caccccgcgc cggtgatcgt tccaccggtg ccgatgccgg caacgaaatg cgtgatcttg ccatcggtat cggcccagat ctccgggccg gtggtcgcgt agtggctggn ccgggccttc ggggttggcg tactggtcgg gcttccacgc accgtcgatt tctctgacca gccggtcaga gacgctgtag tagctgtccg gatcctccgg cggcaccgcc gtcgggcaca ccaccacctc agcgccgtag gcccgcaaga cgtttcgttt gtcctcgctg accttgtcgg ggcagacgaa tacgcacttg tagccccggt gctgggcgac cagggccaaa cccaccccgg tattgcccga cgtcggctcg acgatggtgc cgccgggtcg cagcgcgcca ctggcctcgg ccgcgtcgat catcttgatc gcgatccggt ccttggagct gccgccaggg ttgagatatt cgaccttcgc cgccaccgtg ccgatcccgg ccggaacgac cgcgttcagg cgaaccagtg gtgtgccgcc gatgagatca ctgatgtgct gggcgatccg catcgaacca ggaccctggg tctgagcttc cagggtaccc a
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMRIAQHISD LIGGTPLVRL NAVVPAGIGT VAAKVEYLNP GGSSKDRIAI KMIDAAEASG ALRPGGTIVE PTSGNTGVGL ALVAQHRGYK CVFVCPDKVS EDKRNVLRAY GAEVVVCPTA VPPEDPDSYY SVSDRLVREI DGAWKPDQYA NPEGPASHYA TTGPEIWADT DGKITHFVAG IGTGGTITGA GCYLKEVSGG KVRVIGADPE GSVYSGGTGR PYLVEGVGED FWPATYDHAV PDEIIAVSDS DSFDMTRRLA REEAMLVGGS CGMAVVAALR VAEQAGPDAL VVVLLPDGGR GYMSKIFNDA WMSSYGFLRS RLDGRTDTST VGDVLRRKSG ALPALVHTHP SETVRDAIGI LREYGVSQMP VVGAEPPVMA GEVAGSVSER ELLSAVFSGK AKLADAVSQH MSPPLKLIGA GELVGVAGKA LRDWDALMVV EEGKPVGVIT RYDLLGFLSE GALR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcggatcgcc cagcacatca gtgatctcat cggcggcaca ccactggttc gcctgaacgc ggtcgttccg gccgggatcg gcacggtggc ggcgaaggtc gaatatctca accctggcgg cagctccaag gaccggatcg cgatcaagat gatcgacgcg gccgaggcca gtggcgcgct gcgacccggc ggcaccatcg tcgagccgac gtcgggcaat accggggtgg gtttggccct ggtcgcccag caccggggct acaagtgcgt attcgtctgc cccgacaagg tcagcgagga caaacgaaac gtcttgcggg cctacggcgc tgaggtggtg gtgtgcccga cggcggtgcc gccggaggat ccggacagct actacagcgt ctctgaccgg ctggtcagag aaatcgacgg tgcgtggaag cccgaccagt acgccaaccc cgaaggcccg gccagccact acgcgaccac cggcccggag atctgggccg ataccgatgg caagatcacg catttcgttg ccggcatcgg caccggtgga acgatcaccg gcgcggggtg ctacctcaag gaggtgtcgg gcggcaaagt ccgcgtcatc ggcgccgacc cggaggggtc ggtgtattcc ggcgggaccg ggcggcccta cctggtcgag ggggtgggcg aggacttctg gccggcgacc tacgaccacg cggtgcccga cgagatcatc gcggtgtcgg actccgactc ctttgacatg acccggcggc tggcccgaga agaggcgatg ctggtgggcg gatcctgcgg gatggcggtg gtggccgccc tgcgggtcgc cgaacaagca gggcccgacg cgctggttgt cgtgctgctg cccgacggcg ggcgcggtta catgtcgaag atcttcaacg acgcgtggat gtcgtcgtac ggcttcttgc gcagccgcct cgacggcagg accgatacgt ccacggttgg tgatgtgttg cggcgcaagt ccggtgcgtt gccggctctg gtccacaccc acccctccga gacggtgcgc gatgccatcg gaatcctgcg ggaatatggc gtgtcgcaga tgccggtggt cggcgccgaa ccgccggtga tggccggtga agttgccggc agcgtttccg aacgcgaact gctctcggct gtattctcgg gcaaggccaa gctggccgac gccgtctccc agcacatgag cccgccgctg aagctgatcg gcgccggtga attggtcggt gtggcaggca aggcattgcg ggactgggat gccttgatgg tcgtcgagga gggcaagccg gtcggggtca tcacccggta tgacttgttg ggattccttt ccgaaggggc gttgcggaaa cagcacgaac aagttctgca gccaagcttc tcgaggatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct tatcgatgat aagctgtcaa acatgagaa
Details for MyulA.01147.a.A1.PS01028
PURIFICATION DATe: 4/5/2011
CONCENTRATION: 19.3mg/ml
OBSERVED MW: data unavailable
EXPRESSION LEVEL: Low Expression
PROTEIN PURIFICATION BUFFER: 20 mM HEPES, pH 7.0, 300 mM NaCl, 5% glycerol and 1 mM TCEP
EXPRESSION HOST: data unavailable
VIAL COUNT (approx.): 5
VIAL VOLUME: 100µl
PERCENT IDENTITY: 73
PERCENT COVERAGE: 52
Protocol Notes
notes unavailable
Validated AA Sequence
MRIAQHISDL IGGTPLVRLN AVVPAGIGTV AAKVEYLNPG GSSKDRIAIK MIDAAEASGA LRPGGTIVEP TSGNTGVGLA LVAQHRGYKC VFVCPDKVSE DKRNVLRAYG AEVVVCPTAV PPEDPDSYYS VSDRLVREID GAWKPDQYAN PEGPXQPLRD HRPGDLGRYR WQDHAFRCRH RHRWNDHRRG VLPQGGVGRQ SPRHRRRPGG VGVFRRDRAA LPGRGGGRGL LAGDXRPRGA RRDHRGVGXR LL
Validated NT Sequence
ttaccgcaac gccccttcgg aaaggaatcc caacaagtca taccgggtga tgaccccgac cggcttgccc tcctcgacga ccatcaaggc atcccagtcc cgcaatgcct tgcctgccac accgaccaat tcaccggcgc cgatcagctt cagcggcggg ctcatgtgct gggagacggc gtcggccagc ttggccttgc ccgagaatac agccgagagc agttcgcgtt cggaaacgct gccggcaact tcaccggcca tcaccggcgg ttcggcgccg accaccggca tctgcgacac gccatattcc cgcaggattc cgatggcatc gcgcaccgtc tcggaggggt gggtgtggac cagagccggc aacgcaccgg acttgcgccg caacacatca ccaaccgtgg acgtatcggt cctgccgtcg aggcggctgc gcaagaagcc gtacgacgac atccacgcgt cgttgaagat cttcgacatg taaccgcgcc cgccgtcggg cagcagcacg acaaccagcg cgtcgggccc tgcttgttcg gcgacccgca gggcggccac caccgccatc ccgcaggatc cgcccaccag catcgcctct tctcgggcca gccgccgggt catgtcaaag gagtcggant ccgacaccgc gatgatctcg tcgggcaccg cgtggtcgta ngtcgccggc cagaagtcct cgcccacccc ctcgaccagg tagggccgcc cggtcccgcc ggaatacacc gacccctccg ggtcggcgcc gatgacgcgg actttgccgc ccgacacctc cttgaggtag caccccgcgc cggtgatcgt tccaccggtg ccgatgccgg caacgaaatg cgtgatcttg ccatcggtat cggcccagat ctccgggccg gtggtcgcgt agtggctggn ccgggccttc ggggttggcg tactggtcgg gcttccacgc accgtcgatt tctctgacca gccggtcaga gacgctgtag tagctgtccg gatcctccgg cggcaccgcc gtcgggcaca ccaccacctc agcgccgtag gcccgcaaga cgtttcgttt gtcctcgctg accttgtcgg ggcagacgaa tacgcacttg tagccccggt gctgggcgac cagggccaaa cccaccccgg tattgcccga cgtcggctcg acgatggtgc cgccgggtcg cagcgcgcca ctggcctcgg ccgcgtcgat catcttgatc gcgatccggt ccttggagct gccgccaggg ttgagatatt cgaccttcgc cgccaccgtg ccgatcccgg ccggaacgac cgcgttcagg cgaaccagtg gtgtgccgcc gatgagatca ctgatgtgct gggcgatccg catcgaacca ggaccctggg tctgagcttc cagggtaccc a
Expressed Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMRIAQHISD LIGGTPLVRL NAVVPAGIGT VAAKVEYLNP GGSSKDRIAI KMIDAAEASG ALRPGGTIVE PTSGNTGVGL ALVAQHRGYK CVFVCPDKVS EDKRNVLRAY GAEVVVCPTA VPPEDPDSYY SVSDRLVREI DGAWKPDQYA NPEGPASHYA TTGPEIWADT DGKITHFVAG IGTGGTITGA GCYLKEVSGG KVRVIGADPE GSVYSGGTGR PYLVEGVGED FWPATYDHAV PDEIIAVSDS DSFDMTRRLA REEAMLVGGS CGMAVVAALR VAEQAGPDAL VVVLLPDGGR GYMSKIFNDA WMSSYGFLRS RLDGRTDTST VGDVLRRKSG ALPALVHTHP SETVRDAIGI LREYGVSQMP VVGAEPPVMA GEVAGSVSER ELLSAVFSGK AKLADAVSQH MSPPLKLIGA GELVGVAGKA LRDWDALMVV EEGKPVGVIT RYDLLGFLSE GALR
Full NT Sequence (Expression Vector + Insert)
TTCTTGAAGA CGAAAGGGCC TCGTGATACG CCTATTTTTA TAGGTTAATG TCATGATAAT AATGGTTTCT TAGACGTCAG GTGGCACTTT TCGGGGAAAT GTGCGCGGAA CCCCTATTTG TTTATTTTTC TAAATACATT CAAATATGTA TCCGCTCATG AGACAATAAC CCTGATAAAT GCTTCAATAA TATTGAAAAA GGAAGAGTAT GAGTATTCAA CATTTCCGTG TCGCCCTTAT TCCCTTTTTT GCGGCATTTT GCCTTCCTGT TTTTGCTCAC CCAGAAACGC TGGTGAAAGT AAAAGATGCT GAAGATCAGT TGGGTGCACG AGTGGGTTAC ATCGAACTGG ATCTCAACAG CGGTAAGATC CTTGAGAGTT TTCGCCCCGA AGAACGTTTT CCAATGATGA GCACTTTTAA AGTTCTGCTA TGTGGCGCGG TATTATCCCG TGTTGACGCC GGGCAAGAGC AACTCGGTCG CCGCATACAC TATTCTCAGA ATGACTTGGT TGAGTACTCA CCAGTCACAG AAAAGCATCT TACGGATGGC ATGACAGTAA GAGAATTATG CAGTGCTGCC ATAACCATGA GTGATAACAC TGCGGCCAAC TTACTTCTGA CAACGATCGG AGGACCGAAG GAGCTAACCG CTTTTTTGCA CAACATGGGG GATCATGTAA CTCGCCTTGA TCGTTGGGAA CCGGAGCTGA ATGAAGCCAT ACCAAACGAC GAGCGTGACA CCACGATGCC TGCAGCAATG GCAACAACGT TGCGCAAACT ATTAACTGGC GAACTACTTA CTCTAGCTTC CCGGCAACAA TTAATAGACT GGATGGAGGC GGATAAAGTT GCAGGACCAC TTCTGCGCTC GGCCCTTCCG GCTGGCTGGT TTATTGCTGA TAAATCTGGA GCCGGTGAGC GTGGGTCTCG CGGTATCATT GCAGCACTGG GGCCAGATGG TAAGCCCTCC CGTATCGTAG TTATCTACAC GACGGGGAGT CAGGCAACTA TGGATGAACG AAATAGACAG ATCGCTGAGA TAGGTGCCTC ACTGATTAAG CATTGGTAAC TGTCAGACCA AGTTTACTCA TATATACTTT AGATTGATTT AAAACTTCAT TTTTAATTTA AAAGGATCTA GGTGAAGATC CTTTTTGATA ATCTCATGAC CAAAATCCCT TAACGTGAGT TTTCGTTCCA CTGAGCGTCA GACCCCGTAG AAAAGATCAA AGGATCTTCT TGAGATCCTT TTTTTCTGCG CGTAATCTGC TGCTTGCAAA CAAAAAAACC ACCGCTACCA GCGGTGGTTT GTTTGCCGGA TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA TACTGTCCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC GGTAAGCGGC AGGGTCGGAA CAGGAGAGCG CACGAGGGAG CTTCCAGGGG GAAACGCCTG GTATCTTTAT AGTCCTGTCG GGTTTCGCCA CCTCTGACTT GAGCGTCGAT TTTTGTGATG CTCGTCAGGG GGGCGGAGCC TATGGAAAAA CGCCAGCAAC GCGGCCTTTT TACGGTTCCT GGCCTTTTGC TGGCCTTTTG CTCACATGTT CTTTCCTGCG TTATCCCCTG ATTCTGTGGA TAACCGTATT ACCGCCTTTG AGTGAGCTGA TACCGCTCGC CGCAGCCGAA CGACCGAGCG CAGCGAGTCA GTGAGCGAGG AAGCGGAAGA GCGCCTGATG CGGTATTTTC TCCTTACGCA TCTGTGCGGT ATTTCACACC GCATATATGG TGCACTCTCA GTACAATCTG CTCTGATGCC GCATAGTTAA GCCAGTATAC ACTCCGCTAT CGCTACGTGA CTGGGTCATG GCTGCGCCCC GACACCCGCC AACACCCGCT GACGCGCCCT GACGGGCTTG TCTGCTCCCG GCATCCGCTT ACAGACAAGC TGTGACCGTC TCCGGGAGCT GCATGTGTCA GAGGTTTTCA CCGTCATCAC CGAAACGCGC GAGGCAGCTG CGGTAAAGCT CATCAGCGTG GTCGTGAAGC GATTCACAGA TGTCTGCCTG TTCATCCGCG TCCAGCTCGT TGAGTTTCTC CAGAAGCGTT AATGTCTGGC TTCTGATAAA GCGGGCCATG TTAAGGGCGG TTTTTTCCTG TTTGGTCACT GATGCCTCCG TGTAAGGGGG ATTTCTGTTC ATGGGGGTAA TGATACCGAT GAAACGAGAG AGGATGCTCA CGATACGGGT TACTGATGAT GAACATGCCC GGTTACTGGA ACGTTGTGAG GGTAAACAAC TGGCGGTATG GATGCGGCGG GACCAGAGAA AAATCACTCA GGGTCAATGC CAGCGCTTCG TTAATACAGA TGTAGGTGTT CCACAGGGTA GCCAGCAGCA TCCTGCGATG CAGATCCGGA ACATAATGGT GCAGGGCGCT GACTTCCGCG TTTCCAGACT TTACGAAACA CGGAAACCGA AGACCATTCA TGTTGTTGCT CAGGTCGCAG ACGTTTTGCA GCAGCAGTCG CTTCACGTTC GCTCGCGTAT CGGTGATTCA TTCTGCTAAC CAGTAAGGCA ACCCCGCCAG CCTAGCCGGG TCCTCAACGA CAGGAGCACG ATCATGCGCA CCCGTGGCCA GGACCCAACG CTGCCCGAGA TGCGCCGCGT GCGGCTGCTG GAGATGGCGG ACGCGATGGA TATGTTCTGC CAAGGGTTGG TTTGCGCATT CACAGTTCTC CGCAAGAATT GATTGGCTCC AATTCTTGGA GTGGTGAATC CGTTAGCGAG GTGCCGCCGG CTTCCATTCA GGTCGAGGTG GCCCGGCTCC ATGCACCGCG ACGCAACGCG GGGAGGCAGA CAAGGTATAG GGCGGCGCCT ACAATCCATG CCAACCCGTT CCATGTGCTC GCCGAGGCGG CATAAATCGC CGTGACGATC AGCGGTCCAG TGATCGAAGT TAGGCTGGTA AGAGCCGCGA GCGATCCTTG AAGCTGTCCC TGATGGTCGT CATCTACCTG CCTGGACAGC ATGGCCTGCA ACGCGGGCAT CCCGATGCCG CCGGAAGCGA GAAGAATCAT AATGGGGAAG GCCATCCAGC CTCGCGTCGT GAACGCCAGC AAGACGTAGC CCAGCGCGTC GGCCGTAACA ACACCATTTA AATGGAGTGG TTACAAATGG AGTGGTTAAT TAACAACACC ATTTGTCGAC GCTCTCCCTT ATGCGACTCC TGCATTAGGA AGCAGCCCAG TAGTAGGTTG AGGCCGTTGA GCACCGCCGC CGCAAGGAAT GGTGCATGCA AGGAGATGGC GCCCAACAGT CCCCCGGCCA CGGGGCCTGC CACCATACCC ACGCCGAAAC AAGCGCTCAT GAGCCCGAAG TGGCGAGCCC GATCTTCCCC ATCGGTGATG TCGGCGATAT AGGCGCCAGC AACCGCACCT GTGGCGCCGG TGATGCCGGC CACGATGCGT CCGGCGTAGA GGATCGAGAT CTCGATCCCG CGAAATTAAT ACGACTCACT ATAGGGAGAC CACAACGGTT TCCCTCTAGA AATAATTTTG TTTAACTTTA AGAAGGAGAT ATACCATGGC TCATCACCAT CACCATCATA TGGGTACCCT GGAAGCTCAG ACCCAGGGTC CTGGTTCGAT GCGGATCGCC CAGCACATCA GTGATCTCAT CGGCGGCACA CCACTGGTTC GCCTGAACGC GGTCGTTCCG GCCGGGATCG GCACGGTGGC GGCGAAGGTC GAATATCTCA ACCCTGGCGG CAGCTCCAAG GACCGGATCG CGATCAAGAT GATCGACGCG GCCGAGGCCA GTGGCGCGCT GCGACCCGGC GGCACCATCG TCGAGCCGAC GTCGGGCAAT ACCGGGGTGG GTTTGGCCCT GGTCGCCCAG CACCGGGGCT ACAAGTGCGT ATTCGTCTGC CCCGACAAGG TCAGCGAGGA CAAACGAAAC GTCTTGCGGG CCTACGGCGC TGAGGTGGTG GTGTGCCCGA CGGCGGTGCC GCCGGAGGAT CCGGACAGCT ACTACAGCGT CTCTGACCGG CTGGTCAGAG AAATCGACGG TGCGTGGAAG CCCGACCAGT ACGCCAACCC CGAAGGCCCG GCCAGCCACT ACGCGACCAC CGGCCCGGAG ATCTGGGCCG ATACCGATGG CAAGATCACG CATTTCGTTG CCGGCATCGG CACCGGTGGA ACGATCACCG GCGCGGGGTG CTACCTCAAG GAGGTGTCGG GCGGCAAAGT CCGCGTCATC GGCGCCGACC CGGAGGGGTC GGTGTATTCC GGCGGGACCG GGCGGCCCTA CCTGGTCGAG GGGGTGGGCG AGGACTTCTG GCCGGCGACC TACGACCACG CGGTGCCCGA CGAGATCATC GCGGTGTCGG ACTCCGACTC CTTTGACATG ACCCGGCGGC TGGCCCGAGA AGAGGCGATG CTGGTGGGCG GATCCTGCGG GATGGCGGTG GTGGCCGCCC TGCGGGTCGC CGAACAAGCA GGGCCCGACG CGCTGGTTGT CGTGCTGCTG CCCGACGGCG GGCGCGGTTA CATGTCGAAG ATCTTCAACG ACGCGTGGAT GTCGTCGTAC GGCTTCTTGC GCAGCCGCCT CGACGGCAGG ACCGATACGT CCACGGTTGG TGATGTGTTG CGGCGCAAGT CCGGTGCGTT GCCGGCTCTG GTCCACACCC ACCCCTCCGA GACGGTGCGC GATGCCATCG GAATCCTGCG GGAATATGGC GTGTCGCAGA TGCCGGTGGT CGGCGCCGAA CCGCCGGTGA TGGCCGGTGA AGTTGCCGGC AGCGTTTCCG AACGCGAACT GCTCTCGGCT GTATTCTCGG GCAAGGCCAA GCTGGCCGAC GCCGTCTCCC AGCACATGAG CCCGCCGCTG AAGCTGATCG GCGCCGGTGA ATTGGTCGGT GTGGCAGGCA AGGCATTGCG GGACTGGGAT GCCTTGATGG TCGTCGAGGA GGGCAAGCCG GTCGGGGTCA TCACCCGGTA TGACTTGTTG GGATTCCTTT CCGAAGGGGC GTTGCGGAAA CAGCACGAAC AAGTTCTGCA GCCAAGCTTC TCGAGGATCC GGCTGCTAAC AAAGCCCGAA AGGAAGCTGA GTTGGCTGCT GCCACCGCTG AGCAATAACT AGCATAACCC CTTGGGGCCT CTAAACGGGT CTTGAGGGGT TTTTTGCTGA AAGGAGGAAC TATATCCGGA TATCCACAGG ACGGGTGTGG TCGCCATGAT CGCGTAGTCG ATAGTGGCTC CAAGTAGCGA AGCGAGCAGG ACTGGGCGGC GGCCAAAGCG GTCGGACAGT GCTCCGAGAA CGGGTGCGCA TAGAAATTGC ATCAACGCAT ATAGCGCTAG CAGCACGCCA TAGTGACTGG CGATGCTGTC GGAATGGACG ATATCCCGCA AGAGGCCCGG CAGTACCGGC ATAACCAAGC CTATGCCTAC AGCATCCAGG GTGACGGTGC CGAGGATGAC GATGAGCGCA TTGTTAGATT TCATACACGG TGCCTGACTG CGTTAGCAAT TTAACTGTGA TAAACTACCG CATTAAAGCT TATCGATGAT AAGCTGTCAA ACATGAGAA