MyulA.01117.b

Acyl-CoA dehydrogenase FadE26

CENTER ID: MyulA.01117.b
ORGANISM: Mycobacterium ulcerans Agy99
ASSOCIATED DISEASE: Buruli, Bairnsdale ulcer
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyulA.01117.b.A1.GE29772 Full length( MyulA.01117.b ) 1 392
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|362242.7.peg.4729
RefSeq: YP_907589.1
UniProt: A0PUU9

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MRISYTPEQE ELRRELRSYF TTLMTPERRE ALSSVQGEYG TGNVYRETVA QMGKNGWLTL NWPKEYGGQD RSPMDSLIFT DEAAIAGAPV PFLTINSVAP TIMAFGTDEQ KKFFLPKIAA GDLHFSIGYS EPGAGTDLAN LRTTAVRDGD DYLINGQKMW TSLIAYADWV CLAVRTNPEA KKHRGISMLV VPTTAEGFSW TPVHTMAGPD TSATYYTDVR VPVTNLIGEE NAGWKLVTNQ LNHERVALVS SAPISMALRE VREWAQNTKD AGGARLIDSE WVQLNLARVH AKVEVLKLIN WELASNDAAP SPADASAAKV FGTELATEAY RLLMEVLGTA ATLRQDSPGA LLRGRVERMH RACLILTFGG GTNEVQRDII GMVALGLPRA NR
NT Sequence
ATGCGCATCA GTTACACACC CGAGCAGGAG GAGCTGCGCC GTGAACTGCG GTCGTATTTC ACCACCCTGA TGACGCCGGA GCGCCGCGAG GCGCTGAGTT CGGTCCAGGG CGAATACGGC ACCGGCAACG TCTACCGCGA GACGGTTGCC CAGATGGGCA AGAACGGGTG GCTCACCCTG AACTGGCCGA AGGAATACGG CGGCCAGGAC CGCTCGCCGA TGGACTCGCT GATCTTCACC GACGAGGCGG CCATCGCCGG TGCCCCGGTG CCGTTCCTGA CGATCAACAG CGTGGCGCCG ACCATCATGG CCTTCGGCAC CGACGAGCAG AAGAAGTTCT TCCTCCCCAA GATCGCCGCC GGGGATCTGC ACTTCTCCAT CGGCTACTCC GAGCCGGGCG CGGGCACCGA CCTGGCCAAC CTGCGAACCA CGGCGGTTCG CGACGGCGAC GACTATTTGA TCAACGGCCA GAAGATGTGG ACCAGCCTGA TCGCCTACGC GGACTGGGTC TGCCTGGCGG TGCGCACCAA CCCGGAGGCC AAGAAACACC GCGGCATCTC CATGCTGGTC GTTCCCACGA CCGCCGAAGG CTTCTCGTGG ACACCGGTGC ACACCATGGC CGGACCCGAC ACCAGTGCGA CCTACTACAC CGACGTGCGG GTGCCGGTGA CCAACCTGAT CGGTGAGGAG AACGCGGGCT GGAAACTGGT GACCAACCAG CTCAACCACG AGCGGGTGGC CCTGGTCTCG TCGGCACCGA TATCCATGGC CCTGCGGGAA GTCCGCGAAT GGGCGCAAAA CACCAAGGAT GCCGGCGGCG CCCGTCTCAT CGACTCGGAG TGGGTGCAGC TGAACCTGGC CCGGGTGCAC GCCAAGGTCG AGGTGCTCAA GCTGATCAAC TGGGAGCTGG CCTCGAACGA CGCCGCCCCG TCACCGGCGG ACGCATCGGC CGCCAAGGTG TTCGGCACCG AACTGGCCAC CGAGGCCTAC CGGCTGCTCA TGGAGGTCCT GGGCACCGCG GCCACCCTGC GTCAGGACTC CCCCGGCGCG TTGCTTCGCG GGCGCGTCGA GCGGATGCAC CGGGCGTGTC TGATCCTGAC CTTCGGCGGC GGCACCAACG AAGTCCAGCG CGACATCATC GGCATGGTCG CCCTGGGACT GCCCCGGGCC AACCGC
Details for MyulA.01117.b.A1.GE29772
HARVESTED ON: 6/29/2010
SEQUENCED ON: 7/7/2010
EXPECTED MW: 45kDa
OBSERVED MW: 45kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: High Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 78
PERCENT COVERAGE: 91
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMRISYTPEQ EELRRELRSY FTTLMTPERR EALSSVQGEY GTGNVYRETV AQMGKNGWLT LNWPKEYGGQ DRSPMDSLIF TDEAAIAGAP VPFLTINSVA PTIMAFGTDE QKKFFLPKIA AGDLHFSIGY SEPGAGTDLA NLRTTAVRDG DDYLINGQKM WTSLIAYADW VCLAVRTNPE AKKHRGISML VVPTTAEXFS WTPVHTMAGP DTSATYYTDV RVPVTXLIGE ENAGWKLVTN QLNHERVXLV SSAXISMXLX EVXEWAQNTK XAXXARLIDX XXGXXXXGPX XXXXVRXFXX IXXXXXXXXD AXXXXXGXXX XXXXXXAXXX XXXXXXGXXX XXXXXXXXXX XXXXXP
Validated NT Sequence
ttttntttnn ctttaagaag gaganatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatgcgca tcagttacac acccgagcag gaggagctgc gccgtgaact gcggtcgtat ttcaccaccc tgatgacgcc ggagcgccgc gaggcgctga gttcggtcca gggcgaatac ggcaccggca acgtctaccg cgagacggtt gcccagatgg gcaagaacgg gtggctcacc ctgaactggc cgaaggaata cggcggccag gaccgctcgc cgatggactc gctgatcttc accgacgagg cggccatcgc cggtgccccg gtgccgttcc tgacgatcaa cagcgtggcg ccgaccatca tggccttcgg caccgacgag cagaagaagt tcttcctccc caagatcgcc gccggggatc tgcacttctc catcggctac tccgagccgg gcgcgggcac cgacctggcc aacctgcgaa ccacggcggt tcgcgacggc gacgactatt tgatcaacgg ccagaagatg tggaccagcc tgatcgccta cgcggactgg gtctgcctgg cggtgcgcac caacccggag gccaagaaac accgcggcat ctccatgctg gtcgttccca cgaccgccga angcttctcg tggacaccgg tgcacaccat ggccggaccc gacaccagtg cgacctacta caccgacgtg cgggtgccgg tgaccnacct gatcggtgag gagaacgcgg gctggaaact ggtgaccaac cagctcaacc acgagcgggt gnncctggtc tcgtcggcan ngatatccat ggncctgngg gaagtccncg aatgggcnca aaacaccaag gnngccngcn gcgcccgtct catcgactnn ngantgggnn nanctgannn tgggcccngg tgnncnncna nngtcaggtg nttcangntg atcaannggg nnnnctngnn ntnnnacgac gcccnccnnn nnncngnngg ancnnnnnng ncngncnnng nnnnttnggc nncnnaacnn nnncnnnnnn nnnnnnnngg nnnnnncang nnnnnnnnnn gnnnnnnnnc nnncnnnnnn nntnnnnnnn nnnnnccnnc
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMRISYTPEQ EELRRELRSY FTTLMTPERR EALSSVQGEY GTGNVYRETV AQMGKNGWLT LNWPKEYGGQ DRSPMDSLIF TDEAAIAGAP VPFLTINSVA PTIMAFGTDE QKKFFLPKIA AGDLHFSIGY SEPGAGTDLA NLRTTAVRDG DDYLINGQKM WTSLIAYADW VCLAVRTNPE AKKHRGISML VVPTTAEGFS WTPVHTMAGP DTSATYYTDV RVPVTNLIGE ENAGWKLVTN QLNHERVALV SSAPISMALR EVREWAQNTK DAGGARLIDS EWVQLNLARV HAKVEVLKLI NWELASNDAA PSPADASAAK VFGTELATEA YRLLMEVLGT AATLRQDSPG ALLRGRVERM HRACLILTFG GGTNEVQRDI IGMVALGLPR ANR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcgcatcagt tacacacccg agcaggagga gctgcgccgt gaactgcggt cgtatttcac caccctgatg acgccggagc gccgcgaggc gctgagttcg gtccagggcg aatacggcac cggcaacgtc taccgcgaga cggttgccca gatgggcaag aacgggtggc tcaccctgaa ctggccgaag gaatacggcg gccaggaccg ctcgccgatg gactcgctga tcttcaccga cgaggcggcc atcgccggtg ccccggtgcc gttcctgacg atcaacagcg tggcgccgac catcatggcc ttcggcaccg acgagcagaa gaagttcttc ctccccaaga tcgccgccgg ggatctgcac ttctccatcg gctactccga gccgggcgcg ggcaccgacc tggccaacct gcgaaccacg gcggttcgcg acggcgacga ctatttgatc aacggccaga agatgtggac cagcctgatc gcctacgcgg actgggtctg cctggcggtg cgcaccaacc cggaggccaa gaaacaccgc ggcatctcca tgctggtcgt tcccacgacc gccgaaggct tctcgtggac accggtgcac accatggccg gacccgacac cagtgcgacc tactacaccg acgtgcgggt gccggtgacc aacctgatcg gtgaggagaa cgcgggctgg aaactggtga ccaaccagct caaccacgag cgggtggccc tggtctcgtc ggcaccgata tccatggccc tgcgggaagt ccgcgaatgg gcgcaaaaca ccaaggatgc cggcggcgcc cgtctcatcg actcggagtg ggtgcagctg aacctggccc gggtgcacgc caaggtcgag gtgctcaagc tgatcaactg ggagctggcc tcgaacgacg ccgccccgtc accggcggac gcatcggccg ccaaggtgtt cggcaccgaa ctggccaccg aggcctaccg gctgctcatg gaggtcctgg gcaccgcggc caccctgcgt caggactccc ccggcgcgtt gcttcgcggg cgcgtcgagc ggatgcaccg ggcgtgtctg atcctgacct tcggcggcgg caccaacgaa gtccagcgcg acatcatcgg catggtcgcc ctgggactgc cccgggccaa ccgcaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa