MyulA.01097.e

Short chain alcohol dehydrogenase/reductase

CENTER ID: MyulA.01097.e
ORGANISM: Mycobacterium ulcerans Agy99
ASSOCIATED DISEASE: Buruli, Bairnsdale ulcer
CURRENT STATUS: expressed
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyulA.01097.e.A2.GE29768 Mature protein( MyulA.01097.e ) 26 291
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|362242.7.peg.3954
RefSeq: YP_907047.1
UniProt: A0PTA7

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAQRRYFAGK RCLVTGAASG IGRATALRLA EQGAELYLTD RDGDGLAQTV SAARALGAQV PEHRVLDISD YDEVAAFAAD IHASHPSMDV VLNIAGISAW GTVDRLSHEH WSKMVAVNLM GPIHVIETFV PPMVAAGRGG HLVNVSSAAG LVALPWHAAY SASKYGLRGL SEVLRFDLAR HRIGVSVVVP GAVDTPLVDT VEIAGVDRED PQVNRWVNRF SGHAVSPEKA AEKILAGVAR NRYLVYTSAD IRALYAFKRL AWWPYSVAMR QVNVIFTRAL KPKPVALPQR E
NT Sequence
ATGGCGCAGC GGCGGTATTT CGCGGGAAAG CGGTGTCTTG TCACGGGGGC GGCCAGCGGT ATCGGCCGCG CTACCGCTTT GCGGCTTGCT GAGCAGGGCG CCGAACTGTA TTTGACCGAT CGCGACGGTG ACGGTTTGGC GCAGACCGTG TCGGCGGCCC GCGCGCTCGG TGCGCAGGTG CCCGAGCATC GCGTTCTGGA TATCTCCGAC TACGACGAGG TGGCCGCCTT CGCGGCCGAC ATCCACGCCA GCCACCCCAG CATGGACGTG GTGCTCAACA TCGCCGGCAT TTCGGCGTGG GGGACCGTGG ACCGGCTGTC ACACGAACAC TGGAGCAAGA TGGTCGCCGT CAATCTGATG GGTCCGATCC ATGTGATCGA GACTTTCGTC CCGCCGATGG TTGCGGCTGG TCGGGGCGGG CATTTGGTCA ACGTGTCCTC GGCGGCGGGG CTGGTCGCGC TGCCCTGGCA CGCCGCCTAC AGCGCCAGCA AGTACGGTCT GCGCGGACTG TCTGAAGTGC TGCGCTTCGA CCTGGCGCGC CATCGGATCG GTGTGTCGGT CGTGGTGCCG GGTGCGGTGG ACACCCCATT GGTCGATACG GTCGAAATCG CCGGCGTCGA CCGCGAAGAC CCGCAGGTGA ACCGCTGGGT GAACCGCTTC AGCGGCCATG CCGTTTCGCC GGAAAAGGCC GCCGAGAAGA TCCTGGCCGG GGTCGCCAGA AACAGGTATC TGGTCTACAC CTCGGCTGAT ATCCGCGCGC TTTATGCGTT CAAGCGGCTC GCATGGTGGC CGTACAGCGT GGCGATGCGC CAGGTCAACG TGATCTTCAC GCGCGCGCTC AAACCCAAAC CGGTGGCCCT GCCGCAGCGC GAG
Details for MyulA.01097.e.A2.GE29768
HARVESTED ON: 6/29/2010
SEQUENCED ON: 7/7/2010
EXPECTED MW: 31kDa
OBSERVED MW: 34kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL No Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass
PERCENT IDENTITY: 98
PERCENT COVERAGE: 100
Validated AA Sequence
MAHHHHHHMG TLEAQTQGPG SMALRLAEQG AELYLTDRDG DGLAQTVSAA RALGAQVPEH RVLDISDYDE VAAFAADIHA SHPSMDVVLN IAGISAWGTV DRLSHEHWSK MVAVNLMGPI HVIETFVPPM VAAGRGGHLV NVSSAAGLVA LPWHAAYSAS KYGLRGLSEV LRFDLARHRI GVSVVVPGAV DTPLVDTVEI AGVDREDPQV NRWVNRFSGH AVSPEKAAEK ILAGVARNRY LVYTSADIRA LYAFKRLAWW PYSVAMRQXN VIFTRALKPK PXXLPQRE
Validated NT Sequence
attttgttta nctttaagaa ggaganatac catggctcat caccatcacc atcatatggg taccctggaa gctcagaccc agggtcctgg ttcgatggct ttgcggcttg ctgagcaggg cgccgaactg tatttgaccg atcgcgacgg tgacggtttg gcgcagaccg tgtcggcggc ccgcgcgctc ggtgcgcagg tgcccgagca tcgcgttctg gatatctccg actacgacga ggtggccgcc ttcgcggccg acatccacgc cagccacccc agcatggacg tggtgctcaa catcgccggc atttcggcgt gggggaccgt ggaccggctg tcacacgaac actggagcaa gatggtcgcc gtcaatctga tgggtccgat ccatgtgatc gagactttcg tcccgccgat ggttgcggct ggtcggggcg ggcatttggt caacgtgtcc tcggcggcgg ggctggtcgc gctgccctgg cacgccgcct acagcgccag caagtacggt ctgcgcggac tgtctgaagt gctgcgcttc gacctggcgc gccatcggat cggtgtgtcg gtcgtggtgc cgggtgcggt ggacacccca ttggtcgata cggtcgaaat cgccggcgtc gaccgcgaag acccgcaggt gaaccgctgg gtgaaccgct tcagcggcca tgccgtttcg ccggaaaagg ccgccgagaa gatcctggcc ggggtcgcca gaaacaggta tctggtctac acctcggctg atatccgcgc gctttatgcg ttcaagcggc tcgcatggtg gccgtacagc gtggcgatgc gccaggncaa cgtgatcttc acgcgcgcgc tcaaacccaa accggnggnc ctgccgcagc gcgagtaata acagcacgaa cangtnctgc agccaagctt ctcgaggatn cngctgctan caaagnccga aaggaagctg agttggctgc tgccnnngnt
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMALRLAEQG AELYLTDRDG DGLAQTVSAA RALGAQVPEH RVLDISDYDE VAAFAADIHA SHPSMDVVLN IAGISAWGTV DRLSHEHWSK MVAVNLMGPI HVIETFVPPM VAAGRGGHLV NVSSAAGLVA LPWHAAYSAS KYGLRGLSEV LRFDLARHRI GVSVVVPGAV DTPLVDTVEI AGVDREDPQV NRWVNRFSGH AVSPEKAAEK ILAGVARNRY LVYTSADIRA LYAFKRLAWW PYSVAMRQVN VIFTRALKPK PVALPQRE
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggctttgcgg cttgctgagc agggcgccga actgtatttg accgatcgcg acggtgacgg tttggcgcag accgtgtcgg cggcccgcgc gctcggtgcg caggtgcccg agcatcgcgt tctggatatc tccgactacg acgaggtggc cgccttcgcg gccgacatcc acgccagcca ccccagcatg gacgtggtgc tcaacatcgc cggcatttcg gcgtggggga ccgtggaccg gctgtcacac gaacactgga gcaagatggt cgccgtcaat ctgatgggtc cgatccatgt gatcgagact ttcgtcccgc cgatggttgc ggctggtcgg ggcgggcatt tggtcaacgt gtcctcggcg gcggggctgg tcgcgctgcc ctggcacgcc gcctacagcg ccagcaagta cggtctgcgc ggactgtctg aagtgctgcg cttcgacctg gcgcgccatc ggatcggtgt gtcggtcgtg gtgccgggtg cggtggacac cccattggtc gatacggtcg aaatcgccgg cgtcgaccgc gaagacccgc aggtgaaccg ctgggtgaac cgcttcagcg gccatgccgt ttcgccggaa aaggccgccg agaagatcct ggccggggtc gccagaaaca ggtatctggt ctacacctcg gctgatatcc gcgcgcttta tgcgttcaag cggctcgcat ggtggccgta cagcgtggcg atgcgccagg tcaacgtgat cttcacgcgc gcgctcaaac ccaaaccggt ggccctgccg cagcgcgaga aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a