SSGCID: Target Page

MyulA.01097.a

Ketoacyl reductase

CENTER ID:	MyulA.01097.a
ORGANISM:	Mycobacterium ulcerans Agy99
ASSOCIATED DISEASE:	Buruli, Bairnsdale ulcer
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyulA.01097.a.A1.GE29764	Full length( MyulA.01097.a )		1	267

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|362242.7.peg.5609
RefSeq:	YP_908231.1
UniProt:	A0PWP1

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MALPPPGKDR AAIVTGASSG IGEEFARILS QRGYQVVLVA RSADRLEALA GRLGSDTHPL PADLSVRSDR AGLVDRVAAL GLVPDILINN AGLSTLGPVA KSVPEQEFNL AEVDVAAVVD LCSRFLPAMV ERGRGAVFNV ASVAGFAPLP GQAAYGAAKA FVLSYTHSLR GELHGSGVSV TALSPGPVDT SFGDVAGFTP QETESLPKIM WKSVDQVAQA GIDGLAAGKA VVVPGLANRI SAELIRVTPP DWMGSIMARI HPAMKRQ

NT Sequence

ATGGCTCTCC CACCACCAGG AAAAGACCGC GCCGCCATCG TCACCGGCGC CTCGTCGGGC ATCGGCGAGG AATTCGCCCG GATCCTTTCC CAGCGTGGCT ACCAGGTGGT GTTGGTGGCC CGCAGTGCCG ACCGCCTCGA AGCCCTCGCG GGACGGTTGG GTTCGGATAC CCACCCGCTG CCCGCCGATC TGTCTGTGCG CAGCGACCGC GCCGGCCTGG TCGACCGGGT GGCGGCGCTG GGCCTGGTGC CCGACATCCT GATCAACAAC GCGGGGCTGT CGACGCTGGG TCCGGTGGCG AAATCGGTTC CCGAACAGGA ATTCAACCTG GCCGAGGTCG ATGTGGCCGC GGTGGTCGAC CTGTGCAGCC GGTTTCTGCC GGCCATGGTC GAACGCGGGC GAGGCGCGGT GTTCAACGTG GCGTCGGTGG CCGGATTCGC CCCCCTGCCC GGGCAGGCGG CCTACGGTGC GGCCAAGGCG TTCGTGTTGT CCTACACCCA CAGCCTGCGC GGCGAACTGC ACGGCAGCGG TGTTTCGGTG ACGGCGCTGA GCCCGGGACC GGTGGACACC AGCTTCGGCG ACGTCGCCGG GTTCACCCCG CAGGAAACCG AGAGCTTGCC CAAGATCATG TGGAAGTCCG TCGATCAGGT GGCGCAGGCC GGCATCGACG GTTTGGCAGC CGGCAAGGCC GTCGTCGTCC CCGGTCTTGC CAACCGCATC TCGGCCGAAC TGATCCGGGT AACGCCACCG GATTGGATGG GCTCGATCAT GGCCCGCATC CACCCCGCCA TGAAGCGCCA G

Details for MyulA.01097.a.A1.GE29764

HARVESTED ON:	6/29/2010
SEQUENCED ON:	7/7/2010
EXPECTED MW:	29kDa
OBSERVED MW:	30kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	Low Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass
PERCENT IDENTITY:	95
PERCENT COVERAGE:	108

Validated AA Sequence

MAHHHHHHMG TLEAQTQGPG SMALPPPGKD RAAIVTGASS GIGEEFARIL SQRGYQVVLV ARSADRLEAL AGRLGSDTHP LPADLSVRSD RAGLVDRVAA LGLVPDILIN NAGLSTLGPV AKSVPEQEFN LAEVDVAAVV DLCSRFLPAM VERGRGAVFN VASVAGFAPL PGQAAYGAAK AFVLSYTHSL RGELHGSGVS VTALSPGPVD TSFGDVAGFT PQETESLPKI MWKSVDQVAX AGIDGLAAGK AVVVPGLANR ISAELIRVTP PDWXGLDHGP HPPRHEAPVI NSTNXFCSXA SRGXXCXXSX XGS

Validated NT Sequence

attttgttnn ntttaagaag gaganatacc atggctcatc accatcacca tcatatgggt accctggaag ctcagaccca gggtcctggt tcgatggctc tcccaccacc aggaaaagac cgcgccgcca tcgtcaccgg cgcctcgtcg ggcatcggcg aggaattcgc ccggatcctt tcccagcgtg gctaccaggt ggtgttggtg gcccgcagtg ccgaccgcct cgaagccctc gcgggacggt tgggttcgga tacccacccg ctgcccgccg atctgtctgt gcgcagcgac cgcgccggcc tggtcgaccg ggtggcggcg ctgggcctgg tgcccgacat cctgatcaac aacgcggggc tgtcgacgct gggtccggtg gcgaaatcgg ttcccgaaca ggaattcaac ctggccgagg tcgatgtggc cgcggtggtc gacctgtgca gccggtttct gccggccatg gtcgaacgcg ggcgaggcgc ggtgttcaac gtggcgtcgg tggccggatt cgcccccctg cccgggcagg cggcctacgg tgcggccaag gcgttcgtgt tgtcctacac ccacagcctg cgcggcgaac tgcacggcag cggtgtttcg gtgacggcgc tgagcccggg accggtggac accagcttcg gcgacgtcgc cgggttcacc ccgcaggaaa ccgagagctt gcccaagatc atgtggaagt ccgtcgatca ggtggcgcan gccggcatcg acggtttggc agccggcaag gccgtcgtcg tccccggtct tgccaaccgc atctcggccg aactgatccg ggtaacgcca ccggattggn atgggctcga tcatggcccg catccacccc gccatgaagc gccagtaata aacagcacna acnagttctg cagccnagct tcncgaggnt ncngctgcta nnaaagcncg anaggaagct gantnggctg ctgcnnncgc tgnnnnnnnn nnnta

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMALPPPGKD RAAIVTGASS GIGEEFARIL SQRGYQVVLV ARSADRLEAL AGRLGSDTHP LPADLSVRSD RAGLVDRVAA LGLVPDILIN NAGLSTLGPV AKSVPEQEFN LAEVDVAAVV DLCSRFLPAM VERGRGAVFN VASVAGFAPL PGQAAYGAAK AFVLSYTHSL RGELHGSGVS VTALSPGPVD TSFGDVAGFT PQETESLPKI MWKSVDQVAQ AGIDGLAAGK AVVVPGLANR ISAELIRVTP PDWMGSIMAR IHPAMKRQ

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggctctccca ccaccaggaa aagaccgcgc cgccatcgtc accggcgcct cgtcgggcat cggcgaggaa ttcgcccgga tcctttccca gcgtggctac caggtggtgt tggtggcccg cagtgccgac cgcctcgaag ccctcgcggg acggttgggt tcggataccc acccgctgcc cgccgatctg tctgtgcgca gcgaccgcgc cggcctggtc gaccgggtgg cggcgctggg cctggtgccc gacatcctga tcaacaacgc ggggctgtcg acgctgggtc cggtggcgaa atcggttccc gaacaggaat tcaacctggc cgaggtcgat gtggccgcgg tggtcgacct gtgcagccgg tttctgccgg ccatggtcga acgcgggcga ggcgcggtgt tcaacgtggc gtcggtggcc ggattcgccc ccctgcccgg gcaggcggcc tacggtgcgg ccaaggcgtt cgtgttgtcc tacacccaca gcctgcgcgg cgaactgcac ggcagcggtg tttcggtgac ggcgctgagc ccgggaccgg tggacaccag cttcggcgac gtcgccgggt tcaccccgca ggaaaccgag agcttgccca agatcatgtg gaagtccgtc gatcaggtgg cgcaggccgg catcgacggt ttggcagccg gcaaggccgt cgtcgtcccc ggtcttgcca accgcatctc ggccgaactg atccgggtaa cgccaccgga ttggatgggc tcgatcatgg cccgcatcca ccccgccatg aagcgccaga aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a