SSGCID: Target Page

MyulA.00951.a

Amidase, AmiC_2

CENTER ID:	MyulA.00951.a
ORGANISM:	Mycobacterium ulcerans Agy99
ASSOCIATED DISEASE:	Buruli, Bairnsdale ulcer
CURRENT STATUS:	expressed
COMMUNITY REQUEST:	False
NIH RISK GROUP:	2
SELECT AGENT:	False
NIH PRIORITY pathogens category:	IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER REFERENCE ID	DOMAIN/REGION DESCRIPTION	INFO	AA START	AA STOP	ORDER MATERIAL
MyulA.00951.a.A1.GE29751	Full length( MyulA.00951.a )		1	497

* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE	REFERENCE ID
BV-BRC:	fig\|362242.7.peg.698
RefSeq:	YP_904703.1
UniProt:	A0PLL3

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.

AA Sequence

MHLDEYMSLD ATGLAELVAT KQVRATELLA LARQRADAVN PRLNAIIRRI DALAEGRAAD PELRGPLAGV PFLIKDLDQE YRGFSSSCGS RSLANDVAKQ HALVTQRFLD AGLVVFGMTN TPEFGAKGIT EPDYWGPARN PWNPQHTPGG SSGGSGAAVA AGIVPLAGAN DGGGSIRIPA ACNGLVGLKL SRGLSPYGPQ TGEPLFGMET QGVVSRTVRD SAAIYDAIVG PNPRAAYQVA LPDTTFVEHI KNRPGALKIG YSASSAINAA PDREAVAAVE AAASLLEDLG HRVEQVDPPY DDAALARDFL TIWFAQLHSQ VAEIRKRIGA RNSDFEADTL AMAELGRSTG VLAPLLALDN RNNYIQSLAS FHESYDYFLT PSLATPPLTV GDTTTSPRLQ TAARLLSKLR AGKVLSLSGI MDDLIQESLG WVPYTQLANL TGRPAISLPL HWTPAGLPLG VQFVGGLGSD GDLLALAAQL EEARPWAHRH PATPPAG

NT Sequence

ATGCATCTTG ACGAGTACAT GTCGCTGGAT GCGACTGGCC TGGCCGAGCT GGTGGCGACC AAGCAAGTCC GCGCTACCGA GCTACTTGCT TTGGCGCGTC AGCGCGCCGA CGCGGTCAAC CCGAGGTTGA ACGCGATCAT CCGTCGTATC GATGCCCTGG CTGAGGGACG GGCCGCGGAT CCGGAGCTAC GTGGACCGCT TGCCGGCGTC CCCTTCCTGA TCAAGGACCT CGATCAGGAA TATCGCGGCT TTTCCAGCTC GTGTGGATCT CGGTCGCTGG CCAACGACGT CGCCAAACAA CACGCCCTGG TCACCCAACG ATTCCTGGAC GCGGGCCTGG TCGTCTTCGG CATGACCAAT ACACCCGAGT TCGGGGCCAA GGGCATCACC GAACCCGATT ACTGGGGGCC GGCCCGCAAC CCGTGGAATC CGCAGCACAC CCCGGGCGGC TCCTCAGGCG GATCGGGGGC CGCGGTTGCC GCCGGGATCG TTCCGCTGGC CGGCGCCAAC GACGGCGGCG GATCCATCCG CATTCCCGCG GCCTGCAACG GGCTGGTCGG GCTCAAGCTC AGCCGGGGAT TGTCGCCCTA TGGCCCGCAG ACCGGCGAGC CGCTGTTCGG CATGGAAACC CAGGGGGTGG TTTCGCGCAC CGTTCGCGAC AGCGCCGCCA TCTACGATGC GATCGTCGGG CCCAACCCAC GCGCCGCCTA CCAGGTCGCC CTTCCCGACA CCACCTTCGT CGAACACATC AAGAACCGTC CGGGCGCGCT CAAGATCGGG TACTCGGCGT CGTCTGCGAT CAACGCCGCG CCTGACCGCG AGGCCGTCGC GGCAGTCGAG GCCGCGGCGA GCCTGCTCGA GGACCTGGGC CACCGGGTGG AGCAAGTCGA TCCTCCCTAC GATGACGCCG CGCTGGCCCG CGATTTCCTG ACCATCTGGT TCGCGCAGCT GCACAGCCAA GTCGCCGAGA TCAGAAAGCG AATCGGCGCC CGCAACAGCG ACTTTGAAGC CGACACCTTG GCCATGGCCG AACTCGGCCG ATCCACCGGT GTGCTGGCTC CGCTGCTTGC GCTGGACAAC CGCAACAACT ACATCCAGTC CCTGGCGAGT TTCCATGAGA GTTACGACTA CTTCCTGACT CCCAGCCTGG CGACTCCGCC ACTGACCGTT GGTGACACGA CGACGTCACC ACGCCTGCAG ACGGCGGCGC GGCTGCTGAG CAAGTTGCGT GCCGGAAAGG TGCTCTCGCT CAGCGGGATC ATGGACGATC TCATTCAAGA GAGCCTGGGC TGGGTGCCCT ACACCCAGCT GGCCAATCTG ACCGGACGAC CCGCCATCAG CCTGCCGCTG CACTGGACAC CGGCCGGCCT GCCGTTGGGT GTGCAGTTCG TCGGAGGCCT GGGCAGCGAT GGCGACCTCT TGGCGCTGGC AGCCCAACTC GAAGAGGCCC GTCCCTGGGC GCACCGGCAT CCGGCGACAC CGCCCGCCGG C

Details for MyulA.00951.a.A1.GE29751

HARVESTED ON:	6/29/2010
SEQUENCED ON:	7/7/2010
EXPECTED MW:	54kDa
OBSERVED MW:	55kDa
ANTIBIOTIC MARKER:	ampicillin
COUNT OF EXPRESSION COLONIES:	Many (50-100)
TOTAL EXPRESSION LEVEL:	High Expression
SOLUBLE EXPRESSION LEVEL	No Expression
EXPRESSION HOST:	data unavailable
SEQUENCING RESULT:	pass with incomplete coverage
PERCENT IDENTITY:	96
PERCENT COVERAGE:	56

Validated AA Sequence

Validated NT Sequence

tnnnnnnttt tgtttacttt aagaaggaga nataccatgg ctcatcacca tcaccatcat atgggtaccc tggaagctca gacccagggt cctggttcga tgcatcttga cgagtacatg tcgctggatg cgactggcct ggccgagctg gtggcgacca agcaagtccg cgctaccgag ctacttgctt tggcgcgtca gcgcgccgac gcggtcaacc cgaggttgaa cgcgatcatc cgtcgtatcg atgccctggc tgagggacgg gccgcggatc cggagctacg tggaccgctt gccggcgtcc ccttcctgat caaggacctc gatcaggaat atcgcggctt ttccagctcg tgtggatctc ggtcgctggc caacgacgtc gccaaacaac acgccctggt cacccaacga ttcctggacg cgggcctggt cgtcttcggc atgaccaata cacccgagtt cggggccaag ggcatcaccg aacccgatta ctgggggccg gcccgcaacc cgtggaatcc gcagcacacc ccgggcggct cctcaggcgg atcgggggcc gcggttgccg ccgggatcgt tccgctggcc ggcgccaacg acggcggcgg atccatccgc attcccgcgg cctgcaacgg gctggtcggg ctcaagctca gccggggatt gtcgccctat ggcccgcaga ccggcgagcc gctgttcggc atggaaaccc agggggtggt ttcgcgcacc gttcgcgaca gcgccgccat ctacgatgcg atcgtcgggc ccaacccacg cgccgcctac caggtcgccc ttcccgacac caccttcgtc gaacacatca agaaccgtcc gggcgcgctc aagancgnnn tactcggcgt cgtctgcgat caacgccncg cctgancgcg aggncntcgc ggcagtcnag gccncnnnna gcctgntcga ggacctgggc cnncgggngn nannangnnn atncnnccnt acnatgnnnc cnnnnnnggn ccgnnatttg annatctgnn nnnnnanctg cncannccna nnnnncnann nntcaanaaa gcnnntcggc nccccnnaan ancnannnnn nnnnaannnn nnnngnannn nnnnancnac ngnnnggcng nnnccnnc

Expected Protein Sequence

MAHHHHHHMG TLEAQTQGPG SMHLDEYMSL DATGLAELVA TKQVRATELL ALARQRADAV NPRLNAIIRR IDALAEGRAA DPELRGPLAG VPFLIKDLDQ EYRGFSSSCG SRSLANDVAK QHALVTQRFL DAGLVVFGMT NTPEFGAKGI TEPDYWGPAR NPWNPQHTPG GSSGGSGAAV AAGIVPLAGA NDGGGSIRIP AACNGLVGLK LSRGLSPYGP QTGEPLFGME TQGVVSRTVR DSAAIYDAIV GPNPRAAYQV ALPDTTFVEH IKNRPGALKI GYSASSAINA APDREAVAAV EAAASLLEDL GHRVEQVDPP YDDAALARDF LTIWFAQLHS QVAEIRKRIG ARNSDFEADT LAMAELGRST GVLAPLLALD NRNNYIQSLA SFHESYDYFL TPSLATPPLT VGDTTTSPRL QTAARLLSKL RAGKVLSLSG IMDDLIQESL GWVPYTQLAN LTGRPAISLP LHWTPAGLPL GVQFVGGLGS DGDLLALAAQ LEEARPWAHR HPATPPAG

Full NT Sequence (Expression Vector + Insert)

ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat gcatcttgac gagtacatgt cgctggatgc gactggcctg gccgagctgg tggcgaccaa gcaagtccgc gctaccgagc tacttgcttt ggcgcgtcag cgcgccgacg cggtcaaccc gaggttgaac gcgatcatcc gtcgtatcga tgccctggct gagggacggg ccgcggatcc ggagctacgt ggaccgcttg ccggcgtccc cttcctgatc aaggacctcg atcaggaata tcgcggcttt tccagctcgt gtggatctcg gtcgctggcc aacgacgtcg ccaaacaaca cgccctggtc acccaacgat tcctggacgc gggcctggtc gtcttcggca tgaccaatac acccgagttc ggggccaagg gcatcaccga acccgattac tgggggccgg cccgcaaccc gtggaatccg cagcacaccc cgggcggctc ctcaggcgga tcgggggccg cggttgccgc cgggatcgtt ccgctggccg gcgccaacga cggcggcgga tccatccgca ttcccgcggc ctgcaacggg ctggtcgggc tcaagctcag ccggggattg tcgccctatg gcccgcagac cggcgagccg ctgttcggca tggaaaccca gggggtggtt tcgcgcaccg ttcgcgacag cgccgccatc tacgatgcga tcgtcgggcc caacccacgc gccgcctacc aggtcgccct tcccgacacc accttcgtcg aacacatcaa gaaccgtccg ggcgcgctca agatcgggta ctcggcgtcg tctgcgatca acgccgcgcc tgaccgcgag gccgtcgcgg cagtcgaggc cgcggcgagc ctgctcgagg acctgggcca ccgggtggag caagtcgatc ctccctacga tgacgccgcg ctggcccgcg atttcctgac catctggttc gcgcagctgc acagccaagt cgccgagatc agaaagcgaa tcggcgcccg caacagcgac tttgaagccg acaccttggc catggccgaa ctcggccgat ccaccggtgt gctggctccg ctgcttgcgc tggacaaccg caacaactac atccagtccc tggcgagttt ccatgagagt tacgactact tcctgactcc cagcctggcg actccgccac tgaccgttgg tgacacgacg acgtcaccac gcctgcagac ggcggcgcgg ctgctgagca agttgcgtgc cggaaaggtg ctctcgctca gcgggatcat ggacgatctc attcaagaga gcctgggctg ggtgccctac acccagctgg ccaatctgac cggacgaccc gccatcagcc tgccgctgca ctggacaccg gccggcctgc cgttgggtgt gcagttcgtc ggaggcctgg gcagcgatgg cgacctcttg gcgctggcag cccaactcga agaggcccgt ccctgggcgc accggcatcc ggcgacaccg cccgccggca aacagcacga acaagttctg cagccaagct tctcgaggat ccggctgcta acaaagcccg aaaggaagct gagttggctg ctgccaccgc tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg gatatccaca ggacgggtgt ggtcgccatg atcgcgtagt cgatagtggc tccaagtagc gaagcgagca ggactgggcg gcggccaaag cggtcggaca gtgctccgag aacgggtgcg catagaaatt gcatcaacgc atatagcgct agcagcacgc catagtgact ggcgatgctg tcggaatgga cgatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc aaacatgaga a