MyulA.00896.a

Citrate synthase

CENTER ID: MyulA.00896.a
ORGANISM: Mycobacterium ulcerans Agy99
ASSOCIATED DISEASE: Buruli, Bairnsdale ulcer
CURRENT STATUS: soluble
COMMUNITY REQUEST: False
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyulA.00896.a.A1.GE29746 Full length( MyulA.00896.a ) 1 431
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|362242.7.peg.374
RefSeq: YP_904464.1
UniProt: A0PKX4

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MSDTDDTATL KYPGGEIDLQ IAHATEGADG IALGPLLSKT GHTTFDNGFA NTASCKSSIT YIDGDAGILR YRGYPIDQLA EKSTFIEVCY LLIYGELPNT DQLAQFTGRI QRHTMLHEDL KRFFDGFPRN AHPMPVLSSV VNALSAYYQD ALDPMDSEQV ELSTIRLLAK LPTIAAYAYK KSVGQPFLYP ENSLTLVENF LRMTFGFPAE PYHPDPEVVR ALDMLFILHA DHEQNCSTST VRLVGSSRAN LFTSISGGIN ALWGPLHGGA NQAVLEMLEQ IRDSGDDVSE FVRKVKNREA GVKLMGFGHR VYKNYDPRAR IVKEQADKIL SKLGGDDSLL GIAKEVEEAA LTDDYFIERK LYPNVDFYTG LIYRALAFPT RMFTVLFALG RLPGWIAHWR EMHDEGDSKI GRPRQIYTGY TERDYVAMDG R
NT Sequence
GTGTCCGACA CCGACGACAC CGCAACTCTG AAGTACCCGG GGGGCGAGAT CGACCTGCAG ATCGCCCATG CCACCGAGGG TGCTGACGGC ATTGCGCTCG GCCCGCTGCT GTCCAAGACC GGACACACCA CGTTCGACAA CGGCTTCGCC AATACCGCGT CGTGCAAAAG CTCAATCACC TACATCGACG GTGACGCCGG CATTCTGCGC TACCGCGGAT ACCCGATCGA CCAGCTGGCG GAGAAGTCGA CCTTCATCGA GGTCTGCTAC CTGCTGATCT ACGGCGAGTT GCCCAACACC GACCAGCTCG CCCAGTTCAC CGGCCGGATC CAGCGCCACA CCATGCTGCA CGAGGACCTC AAGCGGTTCT TCGACGGTTT CCCCCGCAAC GCCCACCCGA TGCCGGTGCT GTCCAGCGTG GTCAATGCGC TCTCGGCGTA CTACCAGGAC GCGCTGGACC CGATGGACAG CGAGCAGGTT GAGCTCTCGA CGATCCGGTT GCTGGCCAAG CTGCCGACCA TCGCCGCCTA CGCCTACAAG AAGTCGGTTG GCCAGCCGTT CCTCTACCCG GAGAACTCCC TGACGCTGGT CGAGAACTTC TTGCGGATGA CGTTCGGCTT CCCCGCCGAG CCCTACCACC CCGACCCTGA GGTGGTGCGG GCGCTGGACA TGCTGTTCAT CCTGCACGCC GACCACGAGC AGAACTGCTC GACCTCCACG GTGCGGCTGG TGGGTTCCTC CCGGGCCAAC CTGTTCACCT CGATCTCGGG CGGCATCAAC GCCCTCTGGG GCCCGCTGCA CGGCGGTGCC AACCAGGCGG TGCTCGAGAT GCTCGAGCAG ATCCGCGACA GCGGCGACGA CGTGAGCGAG TTCGTCCGCA AGGTGAAGAA CCGCGAAGCC GGCGTCAAGC TGATGGGCTT TGGCCACCGC GTCTACAAGA ACTACGACCC CCGCGCCAGG ATCGTCAAGG AGCAGGCCGA CAAGATCCTG TCCAAGCTCG GCGGCGATGA CTCCCTGCTG GGTATCGCGA AGGAGGTCGA AGAGGCGGCG TTGACCGACG ACTACTTCAT CGAGCGCAAG CTCTACCCCA ACGTCGACTT CTACACCGGC CTGATCTACC GTGCCCTCGC CTTCCCCACC CGGATGTTCA CCGTGCTGTT TGCGCTGGGC CGGCTGCCGG GCTGGATCGC GCACTGGCGC GAAATGCACG ACGAGGGCGA CAGCAAGATC GGGCGTCCGC GCCAGATCTA CACCGGTTAC ACCGAGCGCG ACTACGTCGC GATGGACGGG CGG
Details for MyulA.00896.a.A1.GE29746
HARVESTED ON: 6/29/2010
SEQUENCED ON: 7/7/2010
EXPECTED MW: 50kDa
OBSERVED MW: 50kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Low Expression
EXPRESSION HOST: data unavailable
SEQUENCING RESULT: pass with incomplete coverage
PERCENT IDENTITY: 100
PERCENT COVERAGE: 95
Validated AA Sequence
MVSDTDDTAT LKYPGGEIDL QIAHATEGAD GIALGPLLSK TGHTTFDNGF ANTASCKSSI TYIDGDAGIL RYRGYPIDQL AEKSTFIEVC YLLIYGELPN TDQLAQFTGR IQRHTMLHED LKRFFDGFPR NAHPMPVLSS VVNALSAYYQ DALDPMDSEQ VELSTIRLLA KLPTIAAYAY KKSVGQPFLY PENSLTLVEN FLRMTFGFPA EPYHPDPEVV RALDMLFILH ADHEQNCSTS TVRLVGSSRA NLFTSISGGI NALWGPLHGG ANQAVLEMLE QIRDSGDDVS EFVRKVKNRE AGVKLMGFGH RVYKNYDPRA RIVKEQADKI LSKLGGDDSL LGIAKEVEEA ALTDDYFIER KLYPNVDFYT GLIYRALAFP TRMFTVLFAL GRLPGWIAHW REMHDEGDSK IGRPRQIYTG YTERDYVAMD GR
Validated NT Sequence
attaccgccc gtccatcgcg acgtagtcgc gctcggtgta accggtgtag atctggcgcg gacgcccgat cttgctgtcg ccctcgtcgt gcatttcgcg ccagtgcgcg atccagcccg gcagccggcc cagcgcaaac agcacggtga acatccgggt ggggaaggcg agggcacggt agatcaggcc ggtgtagaag tcgacgttgg ggtagagctt gcgctcgatg aagtagtcgt cggtcaacgc cgcctcttcg acctccttcg cgatacccag cagggagtca tcgccgccga gcttggacag gatcttgtcg gcctgctcct tgacgatcct ggcgcggggg tcgtagttct tgtagacgcg gtggccaaag cccatcagct tgacgccggc ttcgcggttc ttcaccttgc ggacgaactc gctcacgtcg tcgccgctgt cgcggatctg ctcgagcatc tcgagcaccg cctggttggc accgccgtgc agcgggcccc agagggcgtt gatgccgccc gagatcgagg tgaacaggtt ggcccgggag gaacccacca gccgcaccgt ggaggtcgag cagttctgct cgtggtcggc gtgcaggatg aacagcatgt ccagcgcccg caccacctca gggtcggggt ggtagggctc ggcggggaag ccgaacgtca tccgcaagaa gttctcgacc agcgtcaggg agttctccgg gtagaggaac ggctggccaa ccgacttctt gtaggcgtag gcggcgatgg tcggcagctt ggccagcaac cggatcgtcg agagctcaac ctgctcgctg tccatcgggt ccagcgcgtc ctggtagtac gccgagagcg cattgaccac gctggacagc accggcatcg ggtgggcgtt gcgggggaaa ccgtcgaaga accgcttgag gtcctcgtgc agcatggtgt ggcgctggat ccggccggtg aactgggcga gctggtcggt gttgggcaac tcgccgtaga tcagcaggta gcagacctcg atgaaggtcg acttctccgc cagctggtcg atcgggtatc cgcggtagcg cagaatgccg gcgtcaccgt cgatgtaggt gattgagctt ttgcacgacg cggtattggc gaagccgttg tcgaacgtgg tgtgtccggt cttggacagc agcgggccga gcgcaatgcc gtcagcaccc tcggtggcat gggcgatctg caggtcgatc tcgccccccg ggtacttcag agttgcggtg tcgtcggtgt cggacaccat cgaaccagga ccctgggtct gagcttccag
Expected Protein Sequence
MAHHHHHHMG TLEAQTQGPG SMVSDTDDTA TLKYPGGEID LQIAHATEGA DGIALGPLLS KTGHTTFDNG FANTASCKSS ITYIDGDAGI LRYRGYPIDQ LAEKSTFIEV CYLLIYGELP NTDQLAQFTG RIQRHTMLHE DLKRFFDGFP RNAHPMPVLS SVVNALSAYY QDALDPMDSE QVELSTIRLL AKLPTIAAYA YKKSVGQPFL YPENSLTLVE NFLRMTFGFP AEPYHPDPEV VRALDMLFIL HADHEQNCST STVRLVGSSR ANLFTSISGG INALWGPLHG GANQAVLEML EQIRDSGDDV SEFVRKVKNR EAGVKLMGFG HRVYKNYDPR ARIVKEQADK ILSKLGGDDS LLGIAKEVEE AALTDDYFIE RKLYPNVDFY TGLIYRALAF PTRMFTVLFA LGRLPGWIAH WREMHDEGDS KIGRPRQIYT GYTERDYVAM DGR
Full NT Sequence (Expression Vector + Insert)
ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc gcatatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccag tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgt gaacgccagc aagacgtagc ccagcgcgtc ggccgtaaca acaccattta aatggagtgg ttacaaatgg agtggttaat taacaacacc atttgtcgac gctctccctt atgcgactcc tgcattagga agcagcccag tagtaggttg aggccgttga gcaccgccgc cgcaaggaat ggtgcatgca aggagatggc gcccaacagt cccccggcca cggggcctgc caccataccc acgccgaaac aagcgctcat gagcccgaag tggcgagccc gatcttcccc atcggtgatg tcggcgatat aggcgccagc aaccgcacct gtggcgccgg tgatgccggc cacgatgcgt ccggcgtaga ggatcgagat ctcgatcccg cgaaattaat acgactcact atagggagac cacaacggtt tccctctaga aataattttg tttaacttta agaaggagat ataccatggc tcatcaccat caccatcata tgggtaccct ggaagctcag acccagggtc ctggttcgat ggtgtccgac accgacgaca ccgcaactct gaagtacccg gggggcgaga tcgacctgca gatcgcccat gccaccgagg gtgctgacgg cattgcgctc ggcccgctgc tgtccaagac cggacacacc acgttcgaca acggcttcgc caataccgcg tcgtgcaaaa gctcaatcac ctacatcgac ggtgacgccg gcattctgcg ctaccgcgga tacccgatcg accagctggc ggagaagtcg accttcatcg aggtctgcta cctgctgatc tacggcgagt tgcccaacac cgaccagctc gcccagttca ccggccggat ccagcgccac accatgctgc acgaggacct caagcggttc ttcgacggtt tcccccgcaa cgcccacccg atgccggtgc tgtccagcgt ggtcaatgcg ctctcggcgt actaccagga cgcgctggac ccgatggaca gcgagcaggt tgagctctcg acgatccggt tgctggccaa gctgccgacc atcgccgcct acgcctacaa gaagtcggtt ggccagccgt tcctctaccc ggagaactcc ctgacgctgg tcgagaactt cttgcggatg acgttcggct tccccgccga gccctaccac cccgaccctg aggtggtgcg ggcgctggac atgctgttca tcctgcacgc cgaccacgag cagaactgct cgacctccac ggtgcggctg gtgggttcct cccgggccaa cctgttcacc tcgatctcgg gcggcatcaa cgccctctgg ggcccgctgc acggcggtgc caaccaggcg gtgctcgaga tgctcgagca gatccgcgac agcggcgacg acgtgagcga gttcgtccgc aaggtgaaga accgcgaagc cggcgtcaag ctgatgggct ttggccaccg cgtctacaag aactacgacc cccgcgccag gatcgtcaag gagcaggccg acaagatcct gtccaagctc ggcggcgatg actccctgct gggtatcgcg aaggaggtcg aagaggcggc gttgaccgac gactacttca tcgagcgcaa gctctacccc aacgtcgact tctacaccgg cctgatctac cgtgccctcg ccttccccac ccggatgttc accgtgctgt ttgcgctggg ccggctgccg ggctggatcg cgcactggcg cgaaatgcac gacgagggcg acagcaagat cgggcgtccg cgccagatct acaccggtta caccgagcgc gactacgtcg cgatggacgg gcggaaacag cacgaacaag ttctgcagcc aagcttctcg aggatccggc tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat atccggatat ccacaggacg ggtgtggtcg ccatgatcgc gtagtcgata gtggctccaa gtagcgaagc gagcaggact gggcggcggc caaagcggtc ggacagtgct ccgagaacgg gtgcgcatag aaattgcatc aacgcatata gcgctagcag cacgccatag tgactggcga tgctgtcgga atggacgata tcccgcaaga ggcccggcag taccggcata accaagccta tgcctacagc atccagggtg acggtgccga ggatgacgat gagcgcattg ttagatttca tacacggtgc ctgactgcgt tagcaattta actgtgataa actaccgcat taaagcttat cgatgataag ctgtcaaaca tgagaa