MyulA.00629.d

Bifunctional enzyme MbtA: salicyl-AMP ligase (SAL-AMP ligase) + salicyl-S-ACP synthetase

CENTER ID: MyulA.00629.d
ORGANISM: Mycobacterium ulcerans Agy99
ASSOCIATED DISEASE: Buruli, Bairnsdale ulcer
CURRENT STATUS: expressed
COMMUNITY REQUEST: True
NIH RISK GROUP: 2
SELECT AGENT: False
NIH PRIORITY
pathogens category:		 IIIC

Ordering Clones & Proteins

If there are materials available for this target, they will be listed below. Materials can be ordered from SSGCID using the button in the "order material" column. Clicking the button will add the material to a virtual cart. You may order multiple materials at a time at no cost to you, as this contract is funded by NIAID. When you are ready to place your order, click the "Place Order" link which will appear in the top right corner of the page after you place your first item in your cart.

Clones*

CENTER
REFERENCE ID		 DOMAIN/REGION
DESCRIPTION		 INFO AA
START		 AA
STOP		 ORDER
MATERIAL
MyulA.00629.d.B2.GE40033 ordered domain 3 537
* SSGCID clones represent un-induced expression constructs which have been verified by sequencing from vector primers. Clones may contain a tag, such as N-term 6xHis. Get sequence information using the button in the "info" column.

External Resources

RESOURCE REFERENCE ID
BV-BRC: fig|362242.7.peg.4245
UniProt: A0PTV5

Sequences

These sequences are the native gene sequence; sequences of constructs derived from these sequences may differ due to codon optimization or other protocols. To find the specific sequence of any material you may have ordered, click on the "info" button next to the name of that material.
AA Sequence
MAGFIPYPPE RVASYRAAGY WTGRSIGSLL RQAAARWPDR VGVVDAGASH TFCELDKLAD RVAAGFVSLG IAPGDRVLLQ LPNSCRFAVA LFGLLRAGAI PVMCLPGHRL AELHHFAEVS GAVGLIVAQH AGGFDYRSMA ERLVAAHPQL RHVIIDGESG PFLPWSSLSE GATPDIDVDT TSPALLLVSG GTTGTPKLIP RTHDDYVYNA TASAQLCRLT ADDVYLVSLP AAHNFPLACP GILGSMAVGA TIVFSTDPSP EAAFTLIERH GVTVTALVPA LAKLWTQACD WEPVIPKSLR LLQVGGSRLE PEDARQAREA LTPGLQQVFG MAEGLLNFTR LEDPPDLLEH TQGRPLCSAD ELRIVDGAGA PVAPGAEGEL LVRGPYTING YFCADRDNER CFDPAGFYRS GDLVRLRDDG YLQVTGRVKD VICRCGETIS AQDLEEQLLS HPAIRSAAAV PLPDPDMGEL ICAAIVFAGP PQTPQTLSGL NDYLEQRGVA AHIRIDRLVA MPSLPTTPVG KIDKKAIASQ ILTTTAT
NT Sequence
gtggccgggt tcattccgta cccacccgaa cgagtggcca gctaccgcgc cgccggttat tggaccggtc gcagcatcgg ctcgctgttg cggcaagcgg ccgcacgctg gcctgaccga gtgggtgtgg tcgacgccgg cgccagccat acgttctgcg aactggacaa actggccgat cgagtcgccg cgggcttcgt ttcgctgggc atcgcaccgg gcgaccgggt gctgctgcag ttacccaatt cgtgccggtt cgcggtcgcc ttgttcggcc tgttgcgcgc cggagccatc ccggtgatgt gcctgcccgg acaccggctg gcagaactgc accacttcgc cgaggtcagc ggtgcggtgg gtttgatcgt cgcgcagcac gccggcggct tcgactaccg gtcgatggcc gagcggttgg tcgccgctca cccccagttg cgccacgtca tcatcgacgg cgagtccggg ccctttctgc cgtggtcgtc gctgtccgaa ggcgccacac ccgacatcga tgtcgacacc acatcgcccg ccttgttact cgtctcaggt ggcaccaccg gcactccgaa actgatcccc cgaacacacg acgactacgt ttacaacgcc accgcgagtg cgcagttgtg ccggctgacc gctgacgacg tgtacctcgt gtcgctgccg gcggcgcaca acttcccgtt ggcatgtccg ggcatactcg gctcgatggc cgtcggcgcc accatcgttt tcagcaccga ccccagcccg gaggccgcct tcaccttgat cgagcgccac ggcgtcaccg tcacggcgct ggtacccgcg ctggccaaac tgtggaccca ggcctgtgat tgggagccgg taatcccgaa gtctttgcgg ctattgcaag ttggtggctc cagattggag cccgaggatg ctcgccaggc acgagaggca ttgaccccgg ggctgcaaca ggtcttcggg atggccgaag gactgctgaa cttcacccgc ctcgaagatc cgccggacct cctcgagcac acccagggcc ggcccctgtg ctccgctgac gaattgcgca ttgtcgacgg cgcgggcgcg ccggtggctc ccggcgcaga gggcgagcta ctggtgcgcg gtccctacac catcaacggc tacttctgcg ccgaccgaga caacgagcgc tgcttcgatc cagccggctt ctatcgcagc ggcgacttgg tccgactccg cgacgacggc tacctgcagg tcaccgggcg cgtcaaagac gtcatctgcc gctgcggtga gaccatctcg gcccaggacc tcgaagaaca gttgctcagc cacccggcga tccgctcggc cgcggcggtg ccactgccgg acccggatat gggcgagctg atctgcgctg caattgtttt cgcggggcca ccgcagacgc cgcagacctt gtcgggactc aatgactatc tcgagcagcg tggcgtggcc gcacatatcc gcatcgatcg gctggtcgcg atgccgtcgc tgccgaccac accggtggga aagatcgaca agaaggcgat cgcgagccag atcctgacaa ccacggcgac ctga
Details for MyulA.00629.d.B2.GE40033
HARVESTED ON: 3/24/2016
SEQUENCED ON: 3/25/2016
EXPECTED MW: 58kDa
OBSERVED MW: 60kDa
ANTIBIOTIC MARKER: ampicillin
COUNT OF EXPRESSION COLONIES: Many (50-100)
TOTAL EXPRESSION LEVEL: Moderate Expression
SOLUBLE EXPRESSION LEVEL Insoluble
EXPRESSION HOST: data unavailable
SEQUENCING RESULT:
PERCENT IDENTITY: 99
PERCENT COVERAGE: 98
Validated AA Sequence
MAHHHHHHMG FIPYPPERVA SYRAAGYWTG RSIGSLLRQA AARWPDRVGV VDAGASHTFC ELDKLADRVA AGFVSLGIAP GDRVLLQLPN SCRFAVALFG LLRAGAIPVM CLPGHRLAEL HHFAEVSGAV GLIVAQHAGG FDYRSMAERL VAAHPQLRHV IIDGESGPFL PWSSLSEGAT PDIDVDTTSP ALLLVSGGTT GTPKLIPRTH DDYVYNATAS AQLCRLTADD VYLVSLPAAH NFPLACPGIL GSMAVGATIV FSTDPSPEAX XTLIERHGVT VTALVPALAK LWTQACDWEP VIPKSLRLLQ VGGSRLEPED ARQAREALTP GLQQVFGMAE GLLNFTRLED PPDLLEHTQG RPLCSADELR IVDGAGAPVA PGAEGELLVR GPYTINGYFC ADRDNERCFD PAGFYRSGDL VRLRDDGYLQ VTGRVKDVIC RCGETISAQD LEEQLLSHPA IRSAAAVPLP DPDMGELICA AIVFAGPPQT PQTLSGLNDY LEQRGVAAHI RIDRLVAMPS LPTTPVGKID KKAIAX
Validated NT Sequence
gnctcgcgat cgccttcttg tcgatctttc ccaccggtgt ggtcggcagc gacggcatcg cgaccagccg atcgatgcgg atatgtgcgg ccacgccacg ctgctcgaga tagtcattga gtcccgacaa ggtctgcggc gtctgcggtg gccccgcgaa aacaattgca gcgcagatca gctcgcccat atccgggtcc ggcagtggca ccgccgcggc cgagcggatc gccgggtggc tgagcaactg ttcttcgagg tcctgggccg agatggtctc accgcagcgg cagatgacgt ctttgacgcg cccggtgacc tgcaggtagc cgtcgtcgcg gagtcggacc aagtcgccgc tgcgatagaa gccggctgga tcgaagcagc gctcgttgtc tcggtcggcg cagaagtagc cgttgatggt gtagggaccg cgcaccagta gctcgccctc tgcgccggga gccaccggcg cgcccgcgcc gtcgacaatg cgcaattcgt cagcggagca caggggccgg ccctgggtgt gctcgaggag gtccggcgga tcttcgaggc gggtgaagtt cagcagtcct tcggccatcc cgaagacctg ttgcagcccc ggggtcaatg cctctcgtgc ctggcgagca tcctcgggct ccaatctgga gccaccaact tgcaatagcc gcaaagactt cgggattacc ggctcccaat cacaggcctg ggtccacagt ttggccagcg cgggtaccag cgccgtgacg gtgacgccgt ggcgctcgat caaggtgann ncggcctccg ggctggggtc ggtgctgaaa acgatggtgg cgccgacggc catcgagccg agtatgcccg gacatgccaa cgggaagttg tgcgccgccg gcagcgacac gaggtacacg tcgtcagcgg tcagccggca caactgcgca ctcgcggtgg cgttgtaaac gtagtcgtcg tgtgttcggg ggatcagttt cggagtgccg gtggtgccac ctgagacgag taacaaggcg ggcgatgtgg tgtcgacatc gatgtcgggt gtggcgcctt cggacagcga cgaccacggc agaaagggcc cggactcgcc gtcgatgatg acgtggcgca actgggggtg agcggcgacc aaccgctcgg ccatcgaccg gtagtcgaag ccgccggcgt gctgcgcgac gatcaaaccc accgcaccgc tgacctcggc gaagtggtgc agttctgcca gccggtgtcc gggcaggcac atcaccggga tggctccggc gcgcaacagg ccgaacaagg cgaccgcgaa ccggcacgaa ttgggtaact gcagcagcac ccggtcgccc ggtgcgatgc ccagcgaaac gaagcccgcg gcgactcgat cggccagttt gtccagttcg cagaacgtat ggctggcgcc ggcgtcgacc acacccactc ggtcaggcca gcgtgcggcc gcttgccgca acagcgagcc gatgctgcga ccggtccaat aaccggcggc gcggtagctg gccactcgtt cgggtgggta cggaatgaac cccatatggt ggtggtggtg gtgagccat
Expected Protein Sequence
MAHHHHHHGF IPYPPERVAS YRAAGYWTGR SIGSLLRQAA ARWPDRVGVV DAGASHTFCE LDKLADRVAA GFVSLGIAPG DRVLLQLPNS CRFAVALFGL LRAGAIPVMC LPGHRLAELH HFAEVSGAVG LIVAQHAGGF DYRSMAERLV AAHPQLRHVI IDGESGPFLP WSSLSEGATP DIDVDTTSPA LLLVSGGTTG TPKLIPRTHD DYVYNATASA QLCRLTADDV YLVSLPAAHN FPLACPGILG SMAVGATIVF STDPSPEAAF TLIERHGVTV TALVPALAKL WTQACDWEPV IPKSLRLLQV GGSRLEPEDA RQAREALTPG LQQVFGMAEG LLNFTRLEDP PDLLEHTQGR PLCSADELRI VDGAGAPVAP GAEGELLVRG PYTINGYFCA DRDNERCFDP AGFYRSGDLV RLRDDGYLQV TGRVKDVICR CGETISAQDL EEQLLSHPAI RSAAAVPLPD PDMGELICAA IVFAGPPQTP QTLSGLNDYL EQRGVAAHIR IDRLVAMPSL PTTPVGKIDK KAIASQILTT TAT
Full NT Sequence (Expression Vector + Insert)
taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac tttaagaagg agatatacca tggctcacca ccaccaccac catgggttca ttccgtaccc acccgaacga gtggccagct accgcgccgc cggttattgg accggtcgca gcatcggctc gctgttgcgg caagcggccg cacgctggcc tgaccgagtg ggtgtggtcg acgccggcgc cagccatacg ttctgcgaac tggacaaact ggccgatcga gtcgccgcgg gcttcgtttc gctgggcatc gcaccgggcg accgggtgct gctgcagtta cccaattcgt gccggttcgc ggtcgccttg ttcggcctgt tgcgcgccgg agccatcccg gtgatgtgcc tgcccggaca ccggctggca gaactgcacc acttcgccga ggtcagcggt gcggtgggtt tgatcgtcgc gcagcacgcc ggcggcttcg actaccggtc gatggccgag cggttggtcg ccgctcaccc ccagttgcgc cacgtcatca tcgacggcga gtccgggccc tttctgccgt ggtcgtcgct gtccgaaggc gccacacccg acatcgatgt cgacaccaca tcgcccgcct tgttactcgt ctcaggtggc accaccggca ctccgaaact gatcccccga acacacgacg actacgttta caacgccacc gcgagtgcgc agttgtgccg gctgaccgct gacgacgtgt acctcgtgtc gctgccggcg gcgcacaact tcccgttggc atgtccgggc atactcggct cgatggccgt cggcgccacc atcgttttca gcaccgaccc cagcccggag gccgccttca ccttgatcga gcgccacggc gtcaccgtca cggcgctggt acccgcgctg gccaaactgt ggacccaggc ctgtgattgg gagccggtaa tcccgaagtc tttgcggcta ttgcaagttg gtggctccag attggagccc gaggatgctc gccaggcacg agaggcattg accccggggc tgcaacaggt cttcgggatg gccgaaggac tgctgaactt cacccgcctc gaagatccgc cggacctcct cgagcacacc cagggccggc ccctgtgctc cgctgacgaa ttgcgcattg tcgacggcgc gggcgcgccg gtggctcccg gcgcagaggg cgagctactg gtgcgcggtc cctacaccat caacggctac ttctgcgccg accgagacaa cgagcgctgc ttcgatccag ccggcttcta tcgcagcggc gacttggtcc gactccgcga cgacggctac ctgcaggtca ccgggcgcgt caaagacgtc atctgccgct gcggtgagac catctcggcc caggacctcg aagaacagtt gctcagccac ccggcgatcc gctcggccgc ggcggtgcca ctgccggacc cggatatggg cgagctgatc tgcgctgcaa ttgttttcgc ggggccaccg cagacgccgc agaccttgtc gggactcaat gactatctcg agcagcgtgg cgtggccgca catatccgca tcgatcggct ggtcgcgatg ccgtcgctgc cgaccacacc ggtgggaaag atcgacaaga aggcgatcgc gagccagatc ctgacaacca cggcgacctg agtaagatag gatccggctg ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactagcat aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat ccggatatcc acaggacggg tgtggtcgcc atgatcgcgt agtcgatagt ggctccaagt agcgaagcga gcaggactgg gcggcggcca aagcggtcgg acagtgctcc gagaacgggt gcgcatagaa attgcatcaa cgcatatagc gctagcagca cgccatagtg actggcgatg ctgtcggaat ggacgatatc ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg agaattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgcagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatat atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa tcgccgtgac gatcagcggt ccagtgatcg aagttaggct ggtaagagcc gcgagcgatc cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt tgaaggctct caagggcatc ggtcgacgct ctcccttatg cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc aaggaatggt gcatgcaagg agatggcgcc caacagtccc ccggccacgg ggcctgccac catacccacg ccgaaacaag cgctcatgag cccgaagtgg cgagcccgat cttccccatc ggtgatgtcg gcgatatagg cgccagcaac cgcacctgtg gcgccggtga tgccggccac gatgcgtccg gcgtagagga tcgagatctc gatcccgcga aat